BLASTX nr result
ID: Bupleurum21_contig00028276
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00028276
(962 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004158101.1| PREDICTED: reticuline oxidase-like protein-l... 364 2e-98
ref|XP_004135722.1| PREDICTED: reticuline oxidase-like protein-l... 364 2e-98
ref|XP_002299045.1| predicted protein [Populus trichocarpa] gi|2... 354 2e-95
ref|XP_003637115.1| Reticuline oxidase [Medicago truncatula] gi|... 352 6e-95
ref|XP_002523149.1| Reticuline oxidase precursor, putative [Rici... 350 4e-94
>ref|XP_004158101.1| PREDICTED: reticuline oxidase-like protein-like, partial [Cucumis
sativus]
Length = 513
Score = 364 bits (934), Expect = 2e-98
Identities = 182/330 (55%), Positives = 242/330 (73%), Gaps = 10/330 (3%)
Frame = +3
Query: 3 NVNGEILDRKSMGEDLFWAIRGGIASSFGVIVAWKLKLVVVPEIVTIFSVAKTLEQGATD 182
+VNG++ DRKSMGED+FWAIRG +S+G+++AWK+KL+ VP IVT+F++A+TLEQ ATD
Sbjct: 176 DVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWKIKLLPVPPIVTVFTIARTLEQNATD 235
Query: 183 LVHQWQYVAPKIDRDLYIRVQIKAVNL--NQDGSKRTILVSFESLFLGDADRLLSIMRIS 356
++H+WQYV+ K D L+IR+ + +N +Q G+KRTI +F SLFLG + L+ IM+ +
Sbjct: 236 IIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFNSLFLGKIEELVPIMQKT 295
Query: 357 FPELGLVKDDCREVSWVKSTLWFAGNSAFRFGESVELLLN-RSLIPYLYYKAKSDYVQEP 533
FPELGL K+DC E+SW++S L+FAG F G+ + +LL+ R L P ++KAKSDYV EP
Sbjct: 296 FPELGLTKEDCIEMSWIESVLYFAG---FSRGQPLNVLLDRRPLTPKRFFKAKSDYVNEP 352
Query: 534 MPKKAFEMLWNKYFELEAGAANILMTPYGGIMDDYSASELPFPHRAGNLYMIYSGVSWQA 713
+PK E +W + E EA AA ++++PYGGIMD S SE+PFPHRAGNLY I V W
Sbjct: 353 IPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISESEIPFPHRAGNLYKIQHLVYWD- 411
Query: 714 NTSNGEQ--SKRLNWLRALYSDLAPFVSKNPRASYVNYNDLDLGTG-----SLTYEEASQ 872
GE + +NW+R LYS +APFVSKNPRA+Y+NY DLD+GT + +Y EAS
Sbjct: 412 --EEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDLDIGTNEKNGRNTSYNEASV 469
Query: 873 WGLKYFGVNFKRLVKVKTMVDPDNFFKHEQ 962
WG+KYF NFKRLV VKT VDP NFFK+EQ
Sbjct: 470 WGIKYFKGNFKRLVSVKTKVDPSNFFKNEQ 499
>ref|XP_004135722.1| PREDICTED: reticuline oxidase-like protein-like [Cucumis sativus]
Length = 543
Score = 364 bits (934), Expect = 2e-98
Identities = 182/330 (55%), Positives = 242/330 (73%), Gaps = 10/330 (3%)
Frame = +3
Query: 3 NVNGEILDRKSMGEDLFWAIRGGIASSFGVIVAWKLKLVVVPEIVTIFSVAKTLEQGATD 182
+VNG++ DRKSMGED+FWAIRG +S+G+++AWK+KL+ VP IVT+F++A+TLEQ ATD
Sbjct: 206 DVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWKIKLLPVPPIVTVFTIARTLEQNATD 265
Query: 183 LVHQWQYVAPKIDRDLYIRVQIKAVNL--NQDGSKRTILVSFESLFLGDADRLLSIMRIS 356
++H+WQYV+ K D L+IR+ + +N +Q G+KRTI +F SLFLG + L+ IM+ +
Sbjct: 266 IIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFNSLFLGKIEELVPIMQKT 325
Query: 357 FPELGLVKDDCREVSWVKSTLWFAGNSAFRFGESVELLLN-RSLIPYLYYKAKSDYVQEP 533
FPELGL K+DC E+SW++S L+FAG F G+ + +LL+ R L P ++KAKSDYV EP
Sbjct: 326 FPELGLTKEDCIEMSWIESVLYFAG---FSRGQPLNVLLDRRPLTPKRFFKAKSDYVNEP 382
Query: 534 MPKKAFEMLWNKYFELEAGAANILMTPYGGIMDDYSASELPFPHRAGNLYMIYSGVSWQA 713
+PK E +W + E EA AA ++++PYGGIMD S SE+PFPHRAGNLY I V W
Sbjct: 383 IPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISESEIPFPHRAGNLYKIQHLVYWD- 441
Query: 714 NTSNGEQ--SKRLNWLRALYSDLAPFVSKNPRASYVNYNDLDLGTG-----SLTYEEASQ 872
GE + +NW+R LYS +APFVSKNPRA+Y+NY DLD+GT + +Y EAS
Sbjct: 442 --EEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDLDIGTNEKNGRNTSYNEASV 499
Query: 873 WGLKYFGVNFKRLVKVKTMVDPDNFFKHEQ 962
WG+KYF NFKRLV VKT VDP NFFK+EQ
Sbjct: 500 WGIKYFKGNFKRLVSVKTKVDPSNFFKNEQ 529
>ref|XP_002299045.1| predicted protein [Populus trichocarpa] gi|222846303|gb|EEE83850.1|
predicted protein [Populus trichocarpa]
Length = 533
Score = 354 bits (908), Expect = 2e-95
Identities = 179/321 (55%), Positives = 232/321 (72%), Gaps = 4/321 (1%)
Frame = +3
Query: 12 GEILDRKSMGEDLFWAIRGGIASSFGVIVAWKLKLVVVPEIVTIFSVAKTLEQGATDLVH 191
G ILDR SMGEDLFWAIRGG SFGV+VAWK+ LV VP VT+FSV++TLEQ AT L+H
Sbjct: 210 GRILDRASMGEDLFWAIRGGGGQSFGVVVAWKISLVEVPSTVTMFSVSRTLEQNATKLLH 269
Query: 192 QWQYVAPKIDRDLYIRVQIKAVNLNQDGSKRTILVSFESLFLGDADRLLSIMRISFPELG 371
+WQYVA + DL I VQ+ VN +Q+G+ TI +F SLFLG+ D+LL +M+ SFPELG
Sbjct: 270 RWQYVANTLPEDLVIDVQVTRVNSSQEGN-TTIQATFFSLFLGEVDQLLPVMQESFPELG 328
Query: 372 LVKDDCREVSWVKSTLWFAGNSAFRFGESVELLLNRSLIPYLYYKAKSDYVQEPMPKKAF 551
LVKDDC E+SW++S + G F S+++LLNR+ +KAKSDYV+EPMP+ AF
Sbjct: 329 LVKDDCFEMSWIESVFYTGG---FTSNASLDVLLNRTPRSIPRFKAKSDYVKEPMPEIAF 385
Query: 552 EMLWNKYFELEAGAANILMTPYGGIMDDYSASELPFPHRAGNLYMIYSGVSWQANTSNGE 731
E +W ++FE + A +++ PYGG MD+ S S PFPHRAGNLY++ S VSW+ +
Sbjct: 386 EGIWERFFEEDIEAPTLILIPYGGKMDEISESSTPFPHRAGNLYVLVSSVSWREESKEAS 445
Query: 732 QSKRLNWLRALYSDLAPFVSKNPRASYVNYNDLDLG----TGSLTYEEASQWGLKYFGVN 899
+ + + W+R LYS L +VSKNPR +YVNY DLDLG TG+ +Y++AS WG KYF N
Sbjct: 446 R-RHMAWIRRLYSYLTKYVSKNPREAYVNYRDLDLGINNLTGTTSYKQASIWGRKYFKNN 504
Query: 900 FKRLVKVKTMVDPDNFFKHEQ 962
F RLV+VKT VDP NFF++EQ
Sbjct: 505 FDRLVRVKTEVDPTNFFRNEQ 525
>ref|XP_003637115.1| Reticuline oxidase [Medicago truncatula] gi|355503050|gb|AES84253.1|
Reticuline oxidase [Medicago truncatula]
Length = 576
Score = 352 bits (904), Expect = 6e-95
Identities = 177/325 (54%), Positives = 232/325 (71%), Gaps = 5/325 (1%)
Frame = +3
Query: 3 NVNGEILDRKSMGEDLFWAIRGGIASSFGVIVAWKLKLVVVPEIVTIFSVAKTLEQGATD 182
+VNG +LDR+SMGEDLFWAIRGG +SFGVI+AWK+KLV VP VTIF V++TLE AT
Sbjct: 216 DVNGRVLDRESMGEDLFWAIRGGGGASFGVIIAWKVKLVHVPSTVTIFRVSRTLEHNATK 275
Query: 183 LVHQWQYVAPKIDRDLYIRVQIKAVNLNQDGSKRTILVSFESLFLGDADRLLSIMRISFP 362
L+ +WQ VA K+D DL IRV ++ VN + K TI +FESLFLG D L+ +M FP
Sbjct: 276 LIQKWQLVANKLDEDLNIRVILERVNTSTQTRKLTIKATFESLFLGGVDGLIPLMEEEFP 335
Query: 363 ELGLVKDDCREVSWVKSTLWFAG-NSAFRFGESVELLLNRSLIPYLYYKAKSDYVQEPMP 539
ELGLV++DC E+SW++S ++ G S F + +E+LLNR+ L++KAKSDYV++P+P
Sbjct: 336 ELGLVREDCTEMSWIESVIYHGGFTSDFTSDQPLEVLLNRTHSGVLFFKAKSDYVRDPIP 395
Query: 540 KKAFEMLWNKYFELEAGAANILMTPYGGIMDDYSASELPFPHRAGNLYMIYSGVSWQANT 719
E LW ++E EA +A ++ TPYGGIMD+ S SE+PFPHRAGN+Y I V W+
Sbjct: 396 DIGLEGLWPMFYEDEAKSAVLIFTPYGGIMDEISESEIPFPHRAGNIYQIQHLVFWKEEG 455
Query: 720 SNGEQSKRLNWLRALYSDLAPFVSKNPRASYVNYNDLDLG----TGSLTYEEASQWGLKY 887
E+ + +NW+R LY + PFVSK+PR +YVNY DLD+G G +Y++AS WG+KY
Sbjct: 456 DKVEK-RHINWIRKLYIYMEPFVSKSPRGAYVNYRDLDIGVNNNNGYTSYKKASIWGVKY 514
Query: 888 FGVNFKRLVKVKTMVDPDNFFKHEQ 962
F NFKRL KVKT VDP NFF+ EQ
Sbjct: 515 FKNNFKRLAKVKTKVDPLNFFRSEQ 539
>ref|XP_002523149.1| Reticuline oxidase precursor, putative [Ricinus communis]
gi|223537556|gb|EEF39180.1| Reticuline oxidase precursor,
putative [Ricinus communis]
Length = 469
Score = 350 bits (897), Expect = 4e-94
Identities = 170/323 (52%), Positives = 230/323 (71%), Gaps = 3/323 (0%)
Frame = +3
Query: 3 NVNGEILDRKSMGEDLFWAIRGGIASSFGVIVAWKLKLVVVPEIVTIFSVAKTLEQGATD 182
+VNG +LDR SMGEDLFWAIRGG ++FG++++WK+ LV VP VT+F+V KTL+Q AT
Sbjct: 144 DVNGRVLDRASMGEDLFWAIRGGGGNTFGIVISWKINLVPVPATVTVFTVEKTLKQNATQ 203
Query: 183 LVHQWQYVAPKIDRDLYIRVQIKAVNLNQDGSKRTILVSFESLFLGDADRLLSIMRISFP 362
LV+ WQY+A K+ DL+IRV I+ VN K T+ +F SLFLG DRLL +M+ SFP
Sbjct: 204 LVNGWQYIAEKLHEDLFIRVIIERVNSTSQQGKTTVRAAFNSLFLGGVDRLLPLMQESFP 263
Query: 363 ELGLVKDDCREVSWVKSTLWFAGNSAFRFGESVELLLNRSLIPYLYYKAKSDYVQEPMPK 542
ELGLV++DC +SW++S L+FAG S +++LL+R+ +KAKSDYV+EPMP+
Sbjct: 264 ELGLVREDCINMSWIESILYFAGFS----NSPLDILLSRTQPSVRNFKAKSDYVKEPMPE 319
Query: 543 KAFEMLWNKYFELEAGAANILMTPYGGIMDDYSASELPFPHRAGNLYMIYSGVSWQANTS 722
A E +W + E++ GA ++ +PYGG M + S S +PFPHRAGNLY I W
Sbjct: 320 TALEGIWERLSEMDVGAGQLIFSPYGGRMSEISESSIPFPHRAGNLYKIQHLAYWDEEGI 379
Query: 723 NGEQSKRLNWLRALYSDLAPFVSKNPRASYVNYNDLDLG---TGSLTYEEASQWGLKYFG 893
+ K ++W+R LYS LAP+VSKNPRA+Y+NY DLD+G G+ +Y++AS WG+KYF
Sbjct: 380 VATR-KHISWIRRLYSFLAPYVSKNPRAAYINYRDLDIGINNLGNTSYKQASIWGIKYFK 438
Query: 894 VNFKRLVKVKTMVDPDNFFKHEQ 962
+NF RLV VKT VDP NFF++EQ
Sbjct: 439 INFDRLVHVKTTVDPANFFRNEQ 461