BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00028203
         (1300 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002279067.1| PREDICTED: uncharacterized protein LOC100259...   624   e-176
ref|XP_002516816.1| conserved hypothetical protein [Ricinus comm...   615   e-174
ref|XP_003550090.1| PREDICTED: MHD domain-containing death-induc...   610   e-172
ref|NP_850004.1| clathrin adaptor complexes medium subunit famil...   600   e-169
ref|NP_973495.1| clathrin adaptor complexes medium subunit famil...   600   e-169

>ref|XP_002279067.1| PREDICTED: uncharacterized protein LOC100259662 [Vitis vinifera]
            gi|297736956|emb|CBI26157.3| unnamed protein product
            [Vitis vinifera]
          Length = 627

 Score =  624 bits (1609), Expect = e-176
 Identities = 311/433 (71%), Positives = 358/433 (82%), Gaps = 3/433 (0%)
 Frame = +3

Query: 9    AFMVAHAIGDIITGDSLEPEVVVNVGPSVGGLLDSLTGSIGIXXXXXXXXXXXXXXXXXG 188
            A MVAHAIGD+ITGD +EPEVVV+  PSVGGLLDSLTGSIGI                  
Sbjct: 176  ACMVAHAIGDVITGDVVEPEVVVSASPSVGGLLDSLTGSIGISGISTRTKPVAAPVAAST 235

Query: 189  TSVT---GALSADVPKTGARLLDKDALRTYISIAMPFGTPLDLNYSNVSAIKSNGFLSTD 359
            TS T   GA+++D PK G+R LDKDALRT+I+ +MPFGTPLDL+YSN+ AIK NGF S+D
Sbjct: 236  TSSTAVIGAVTSDAPKFGSRPLDKDALRTFINSSMPFGTPLDLSYSNIFAIKVNGFSSSD 295

Query: 360  LPPADRKQPAWKPYMYKGKQRILFTIHETVNAAMYDRDEIPDNISISGQVNCRAELEGLP 539
            LP  D KQPAWKPY+YKGKQR+LFTIHETV+AAMYDRDEIPD+ISISGQVNCRAELEGLP
Sbjct: 296  LPLPDLKQPAWKPYLYKGKQRMLFTIHETVHAAMYDRDEIPDSISISGQVNCRAELEGLP 355

Query: 540  DVLFPLTGLDTARVEVLSFHPCAQVPEQGGDKQAVMFTPPLGNFTLMRYQAFCGIGPPIK 719
            DV FPLTGL+ A +EVLSFHPCAQVPEQG DKQAVMF+PPLGNF LM YQAFCG+GPP+K
Sbjct: 356  DVSFPLTGLNKAGIEVLSFHPCAQVPEQGVDKQAVMFSPPLGNFVLMHYQAFCGLGPPVK 415

Query: 720  GFYQLSMVSENEGAFLFKLRLMEGYKAPLTMEVCSVTMPFPRRRVIHFDGTPSVGTVSYT 899
            GFYQLSMVSE+EGAFLFKL LMEGYKAPLTME C+VTMPFPRRRV+ FDGTPS+GTVS T
Sbjct: 416  GFYQLSMVSEDEGAFLFKLCLMEGYKAPLTMEFCTVTMPFPRRRVVSFDGTPSIGTVSTT 475

Query: 900  DHSVEWKILTSGRGVSGKSIEATFPGSVKFAPWQQKSGSSGSRNGXXXXXXXXXXXXXXX 1079
            +H VEWKI+T GRG++G+SIEATFPG++KFAPWQ +   S SR+                
Sbjct: 476  EHLVEWKIITGGRGLTGRSIEATFPGTIKFAPWQIQRLPS-SRSFLGADEDSDFETDSTN 534

Query: 1080 NMVNVEDFVMEKMNKDLKSVDLEEPFCWQAYNYAKISFKMVGASLSGMSIETKSINIFPP 1259
            NMVNVE+F+MEKM+KDL   DLEEPFCWQAYNYAK++FK+VGASLSGMSI+ KS++I+P 
Sbjct: 535  NMVNVEEFLMEKMSKDLPPADLEEPFCWQAYNYAKVTFKIVGASLSGMSIDPKSVSIYPA 594

Query: 1260 IKAPVEFSTQVIS 1298
            +KAPVEFS+QV S
Sbjct: 595  VKAPVEFSSQVTS 607


>ref|XP_002516816.1| conserved hypothetical protein [Ricinus communis]
            gi|223543904|gb|EEF45430.1| conserved hypothetical
            protein [Ricinus communis]
          Length = 632

 Score =  615 bits (1587), Expect = e-174
 Identities = 303/434 (69%), Positives = 356/434 (82%), Gaps = 4/434 (0%)
 Frame = +3

Query: 9    AFMVAHAIGDIITGDSLEPEVVVNVGPSVGGLLDSLTGSIGIXXXXXXXXXXXXXXXXX- 185
            AFMVA AIGDII+GD ++PEVVV+V PSVGGLLDSLTGSIGI                  
Sbjct: 181  AFMVALAIGDIISGDVVDPEVVVSVSPSVGGLLDSLTGSIGISGISSRAKPVAAPVASAT 240

Query: 186  --GTSVTGALSADVPKTGARLLDKDALRTYISIAMPFGTPLDLNYSNVSAIKSNGFLSTD 359
               T+ TG++++D PK G+RLLDK+ALR +IS AMPFGTPLDLN+SNV AIK+NGF S+D
Sbjct: 241  PFSTAATGSITSDAPKIGSRLLDKEALRNFISSAMPFGTPLDLNFSNVFAIKANGFSSSD 300

Query: 360  LPPADRKQPAWKPYMYKGKQRILFTIHETVNAAMYDRDEIPDNISISGQVNCRAELEGLP 539
            LPP+D KQPAWKPY+YKGKQRILFT+HETV+AAMYDRDEI D ISISGQ+NCRAELEGLP
Sbjct: 301  LPPSDLKQPAWKPYLYKGKQRILFTVHETVHAAMYDRDEISDTISISGQINCRAELEGLP 360

Query: 540  DVLFPLTGLDTARVEVLSFHPCAQVPEQGGDKQAVMFTPPLGNFTLMRYQAFCGIGPPIK 719
            DV  PLTGL+ A VEVLSFHPCAQ PEQ  DKQA+MF+PPLGNF L+RYQA C IGPPI 
Sbjct: 361  DVSLPLTGLNKAHVEVLSFHPCAQAPEQAADKQAMMFSPPLGNFVLVRYQANCAIGPPIM 420

Query: 720  GFYQLSMVSENEGAFLFKLRLMEGYKAPLTMEVCSVTMPFPRRRVIHFDGTPSVGTVSYT 899
            GFYQLSMVSE+EGAFLFKLR+MEGYKAPLTME C+VTMPFPRR+V+ FDGTPS+G VS T
Sbjct: 421  GFYQLSMVSEDEGAFLFKLRIMEGYKAPLTMEFCNVTMPFPRRKVVSFDGTPSLGAVSTT 480

Query: 900  DHSVEWKILTSGRGVSGKSIEATFPGSVKFAPWQ-QKSGSSGSRNGXXXXXXXXXXXXXX 1076
            +HS+EWKI+ SGR ++GKSIEATFPG+++FAPWQ Q+  SS + +G              
Sbjct: 481  EHSIEWKIIPSGRSLTGKSIEATFPGTIRFAPWQTQRLPSSKTGSGDMSDGDSDVEAEST 540

Query: 1077 XNMVNVEDFVMEKMNKDLKSVDLEEPFCWQAYNYAKISFKMVGASLSGMSIETKSINIFP 1256
             NMVNVE+F+M+KM+KDL  VDLEEPFCWQAYNYAK+SFK+ GAS+SGMSI+ KS++I+P
Sbjct: 541  NNMVNVEEFLMDKMSKDLPPVDLEEPFCWQAYNYAKVSFKITGASVSGMSIDPKSVSIYP 600

Query: 1257 PIKAPVEFSTQVIS 1298
             +KAPVE STQV S
Sbjct: 601  AVKAPVELSTQVTS 614


>ref|XP_003550090.1| PREDICTED: MHD domain-containing death-inducing protein-like [Glycine
            max]
          Length = 627

 Score =  610 bits (1572), Expect = e-172
 Identities = 297/431 (68%), Positives = 353/431 (81%), Gaps = 1/431 (0%)
 Frame = +3

Query: 9    AFMVAHAIGDIITGDSLEPEVVVNVGPSVGGLLDSLTGSIGIXXXXXXXXXXXXXXXXXG 188
            AFM+AHAIGDIITGD++EPEV+V+  PSVGGL DSLTGSIGI                  
Sbjct: 178  AFMIAHAIGDIITGDTVEPEVIVSAAPSVGGLFDSLTGSIGISSRAKPVAPPVASSSPSS 237

Query: 189  TSVTGALSADVPKTGARLLDKDALRTYISIAMPFGTPLDLNYSNVSAIKSNGFLSTDLPP 368
             +V G+++AD PK G+RLLDKDALRT+IS +MPFGTPLDLNYSN+  IK+NGF +TDLPP
Sbjct: 238  AAVPGSVTADAPKMGSRLLDKDALRTFISSSMPFGTPLDLNYSNIITIKTNGFSATDLPP 297

Query: 369  ADRKQPAWKPYMYKGKQRILFTIHETVNAAMYDRDEIPDNISISGQVNCRAELEGLPDVL 548
            AD+KQPAWKPY+YKGKQR+LFTIHE ++AA+YDRDEIPD IS+SGQ+NCRA+LEGLPDV 
Sbjct: 298  ADQKQPAWKPYLYKGKQRMLFTIHEIIHAALYDRDEIPDTISVSGQINCRADLEGLPDVS 357

Query: 549  FPLTGLDTARVEVLSFHPCAQVPEQGGDKQAVMFTPPLGNFTLMRYQAFCGIGPPIKGFY 728
            F L GL+TA +EVLS+HPCAQV +QG DKQ VMF+PPLGNF LMRYQA   +GPPIKGFY
Sbjct: 358  FHLAGLNTANLEVLSYHPCAQVSDQGLDKQGVMFSPPLGNFVLMRYQAAYALGPPIKGFY 417

Query: 729  QLSMVSENEGAFLFKLRLMEGYKAPLTMEVCSVTMPFPRRRVIHFDGTPSVGTVSYTDHS 908
            QLSMVSE++GAFLFKL LMEGYKAPLTME C+VTMPFPRRR++  DGTPSVGTVS ++HS
Sbjct: 418  QLSMVSEDKGAFLFKLHLMEGYKAPLTMEFCTVTMPFPRRRIVSLDGTPSVGTVSTSEHS 477

Query: 909  VEWKILTSGRGVSGKSIEATFPGSVKFAPWQ-QKSGSSGSRNGXXXXXXXXXXXXXXXNM 1085
            VEWKI+TSGRG++GKSIE TFPG+VKFAPWQ Q+  SS S  G               NM
Sbjct: 478  VEWKIVTSGRGLTGKSIEVTFPGTVKFAPWQTQRLSSSRSSFGITADEDSDNEAENASNM 537

Query: 1086 VNVEDFVMEKMNKDLKSVDLEEPFCWQAYNYAKISFKMVGASLSGMSIETKSINIFPPIK 1265
            VN E+ +M KMNK L  VDLEEPFCWQAYNYAK+SFK+VGAS+SG++++ KS++I+P +K
Sbjct: 538  VN-EEHLMGKMNKGLPPVDLEEPFCWQAYNYAKVSFKIVGASVSGVAVDPKSVSIYPAVK 596

Query: 1266 APVEFSTQVIS 1298
            AP+EFSTQV S
Sbjct: 597  APMEFSTQVTS 607


>ref|NP_850004.1| clathrin adaptor complexes medium subunit family protein [Arabidopsis
            thaliana] gi|18087614|gb|AAL58937.1|AF462850_1
            At2g20790/F5H14.24 [Arabidopsis thaliana]
            gi|19699154|gb|AAL90943.1| At2g20790/F5H14.24
            [Arabidopsis thaliana] gi|330251979|gb|AEC07073.1|
            clathrin adaptor complexes medium subunit family protein
            [Arabidopsis thaliana]
          Length = 613

 Score =  600 bits (1547), Expect = e-169
 Identities = 286/430 (66%), Positives = 345/430 (80%)
 Frame = +3

Query: 9    AFMVAHAIGDIITGDSLEPEVVVNVGPSVGGLLDSLTGSIGIXXXXXXXXXXXXXXXXXG 188
            AFMVAHA GDII+GD++EPEVVV+V PSVGGL DSLTGSIGI                 G
Sbjct: 174  AFMVAHAFGDIISGDTVEPEVVVSVSPSVGGLFDSLTGSIGISSRAKPVAAPVASSNPSG 233

Query: 189  TSVTGALSADVPKTGARLLDKDALRTYISIAMPFGTPLDLNYSNVSAIKSNGFLSTDLPP 368
             ++TGA ++D PK G+RLLD+D LR +I+ AMPFGTPLDL+ SN+SA+K+NGF S D PP
Sbjct: 234  AAITGATASDAPKAGSRLLDRDLLRNFIATAMPFGTPLDLSLSNISAMKANGFSSADPPP 293

Query: 369  ADRKQPAWKPYMYKGKQRILFTIHETVNAAMYDRDEIPDNISISGQVNCRAELEGLPDVL 548
             + KQPAWKPY+YKGKQR+LFTIHETV+AAMYDRDEIPDN+S++GQ+NCRAELEGLPDV 
Sbjct: 294  QELKQPAWKPYLYKGKQRLLFTIHETVSAAMYDRDEIPDNVSVAGQINCRAELEGLPDVS 353

Query: 549  FPLTGLDTARVEVLSFHPCAQVPEQGGDKQAVMFTPPLGNFTLMRYQAFCGIGPPIKGFY 728
            FPL GL TA +E +SFHPCAQVP  G DKQ ++F PPLGNF LMRYQA CG+GPP+KGFY
Sbjct: 354  FPLAGLSTAHIEAISFHPCAQVPAHGIDKQNIVFQPPLGNFVLMRYQAGCGLGPPVKGFY 413

Query: 729  QLSMVSENEGAFLFKLRLMEGYKAPLTMEVCSVTMPFPRRRVIHFDGTPSVGTVSYTDHS 908
            QLSMVSE+EGAFLFK+ LMEGYKAPL+ME C++TMPFPRRR++ FDGTPS GTV  T+HS
Sbjct: 414  QLSMVSEDEGAFLFKVHLMEGYKAPLSMEFCTITMPFPRRRIVAFDGTPSAGTVLTTEHS 473

Query: 909  VEWKILTSGRGVSGKSIEATFPGSVKFAPWQQKSGSSGSRNGXXXXXXXXXXXXXXXNMV 1088
            VEW+IL SGR +SGKS+EATFPG++KF+P Q +    G                   N+V
Sbjct: 474  VEWRILGSGRSLSGKSLEATFPGTIKFSPLQSRRKGDGD--------DEESEDESAENVV 525

Query: 1089 NVEDFVMEKMNKDLKSVDLEEPFCWQAYNYAKISFKMVGASLSGMSIETKSINIFPPIKA 1268
            NVEDF+++KMNKDL + +LEEPFCWQAY+YAK+SFK+VGAS+S MSI+TKS+NI+P  K+
Sbjct: 526  NVEDFLVQKMNKDLPAAELEEPFCWQAYDYAKVSFKIVGASVSRMSIDTKSVNIYPTTKS 585

Query: 1269 PVEFSTQVIS 1298
            PVEFS QV S
Sbjct: 586  PVEFSAQVTS 595


>ref|NP_973495.1| clathrin adaptor complexes medium subunit family protein [Arabidopsis
            thaliana] gi|4454469|gb|AAD20916.1| unknown protein
            [Arabidopsis thaliana] gi|330251977|gb|AEC07071.1|
            clathrin adaptor complexes medium subunit family protein
            [Arabidopsis thaliana]
          Length = 475

 Score =  600 bits (1547), Expect = e-169
 Identities = 286/430 (66%), Positives = 345/430 (80%)
 Frame = +3

Query: 9    AFMVAHAIGDIITGDSLEPEVVVNVGPSVGGLLDSLTGSIGIXXXXXXXXXXXXXXXXXG 188
            AFMVAHA GDII+GD++EPEVVV+V PSVGGL DSLTGSIGI                 G
Sbjct: 36   AFMVAHAFGDIISGDTVEPEVVVSVSPSVGGLFDSLTGSIGISSRAKPVAAPVASSNPSG 95

Query: 189  TSVTGALSADVPKTGARLLDKDALRTYISIAMPFGTPLDLNYSNVSAIKSNGFLSTDLPP 368
             ++TGA ++D PK G+RLLD+D LR +I+ AMPFGTPLDL+ SN+SA+K+NGF S D PP
Sbjct: 96   AAITGATASDAPKAGSRLLDRDLLRNFIATAMPFGTPLDLSLSNISAMKANGFSSADPPP 155

Query: 369  ADRKQPAWKPYMYKGKQRILFTIHETVNAAMYDRDEIPDNISISGQVNCRAELEGLPDVL 548
             + KQPAWKPY+YKGKQR+LFTIHETV+AAMYDRDEIPDN+S++GQ+NCRAELEGLPDV 
Sbjct: 156  QELKQPAWKPYLYKGKQRLLFTIHETVSAAMYDRDEIPDNVSVAGQINCRAELEGLPDVS 215

Query: 549  FPLTGLDTARVEVLSFHPCAQVPEQGGDKQAVMFTPPLGNFTLMRYQAFCGIGPPIKGFY 728
            FPL GL TA +E +SFHPCAQVP  G DKQ ++F PPLGNF LMRYQA CG+GPP+KGFY
Sbjct: 216  FPLAGLSTAHIEAISFHPCAQVPAHGIDKQNIVFQPPLGNFVLMRYQAGCGLGPPVKGFY 275

Query: 729  QLSMVSENEGAFLFKLRLMEGYKAPLTMEVCSVTMPFPRRRVIHFDGTPSVGTVSYTDHS 908
            QLSMVSE+EGAFLFK+ LMEGYKAPL+ME C++TMPFPRRR++ FDGTPS GTV  T+HS
Sbjct: 276  QLSMVSEDEGAFLFKVHLMEGYKAPLSMEFCTITMPFPRRRIVAFDGTPSAGTVLTTEHS 335

Query: 909  VEWKILTSGRGVSGKSIEATFPGSVKFAPWQQKSGSSGSRNGXXXXXXXXXXXXXXXNMV 1088
            VEW+IL SGR +SGKS+EATFPG++KF+P Q +    G                   N+V
Sbjct: 336  VEWRILGSGRSLSGKSLEATFPGTIKFSPLQSRRKGDGD--------DEESEDESAENVV 387

Query: 1089 NVEDFVMEKMNKDLKSVDLEEPFCWQAYNYAKISFKMVGASLSGMSIETKSINIFPPIKA 1268
            NVEDF+++KMNKDL + +LEEPFCWQAY+YAK+SFK+VGAS+S MSI+TKS+NI+P  K+
Sbjct: 388  NVEDFLVQKMNKDLPAAELEEPFCWQAYDYAKVSFKIVGASVSRMSIDTKSVNIYPTTKS 447

Query: 1269 PVEFSTQVIS 1298
            PVEFS QV S
Sbjct: 448  PVEFSAQVTS 457