BLASTX nr result
ID: Bupleurum21_contig00027540
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00027540
(1504 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002264839.2| PREDICTED: probable serine/threonine-protein... 619 e-175
ref|XP_002533444.1| eif2alpha kinase, putative [Ricinus communis... 579 e-163
ref|XP_003540698.1| PREDICTED: probable serine/threonine-protein... 557 e-156
ref|XP_002876502.1| kinase family protein [Arabidopsis lyrata su... 555 e-156
ref|NP_191500.2| non-specific serine/threonine protein kinase [A... 553 e-155
>ref|XP_002264839.2| PREDICTED: probable serine/threonine-protein kinase GCN2-like [Vitis
vinifera]
Length = 1244
Score = 619 bits (1596), Expect = e-175
Identities = 332/514 (64%), Positives = 377/514 (73%), Gaps = 13/514 (2%)
Frame = +1
Query: 1 DGRGHEYGTFEKTTGPDVMPL-IQVTRQGPLRNLSGKAALRTATSKLEILDEKSEDESES 177
DG H YG K + PL +Q T QG L + T KL+ L+E+ ED+SES
Sbjct: 221 DGSKHGYGIEGKKLDKNTKPLTMQNTNQGQLPS---------PTVKLDALEEEIEDDSES 271
Query: 178 I---GTCTDQSDESDGDATASEDTIAD-----EHLSETYNDLKS-SEESVSLASILHDQS 330
I G+ +E G+ T + D + E D++S + ES+S S++HDQ+
Sbjct: 272 ISFFGSSRSLREELAGNVTTEKQENKDFSPEEDTAEEDDGDIESDASESLSYVSVIHDQT 331
Query: 331 SQTVARDLVLAHLLRVVCAPKGPLADALPEITTELYNLGIVSREVQDYALKPAPLFDRTF 510
S TV +DL++ HLLR+ CA KG LADALPEITTELYNLGI S V+D A KP+ F++TF
Sbjct: 332 SHTVEKDLLMVHLLRLACASKGGLADALPEITTELYNLGIFSEPVRDLATKPSSFFNKTF 391
Query: 511 NHVFGQHMVSSKNTNFWMAASDIREQNSSTTPSSRYLNDFEELQPLGHGGFGHVVLCKNK 690
+HVF QH+VSS+ + FW SD Q++S PSSRYLNDFEELQPLGHGGFGHVVLCKNK
Sbjct: 392 DHVFRQHVVSSRISQFWKPPSDFGGQSTSL-PSSRYLNDFEELQPLGHGGFGHVVLCKNK 450
Query: 691 LDGRQYAMKKIRLKDKSPPLNDRIVREVATLSRLQHQHVVRYYQAWFEAGVFGH-DNGPW 867
LDGRQYA+KKIRLKDKSPP+ DRI+REVATLSRLQHQHVVRYYQAWFE GV G + W
Sbjct: 451 LDGRQYAVKKIRLKDKSPPVYDRILREVATLSRLQHQHVVRYYQAWFETGVSGSFGDTTW 510
Query: 868 GSESARSSGFSYIDASSTDFTEND-KLESTYLYIQMEYCPRTLRQMFESYDHFDKELAWH 1044
GS + SS FSY ASS D ++ KLESTYLYIQMEYCPRTLRQMFESY HFDKELAWH
Sbjct: 511 GSMTPASSSFSYKGASSADVNVHENKLESTYLYIQMEYCPRTLRQMFESYSHFDKELAWH 570
Query: 1045 LSRQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGXXXXXXXXXXXXXXXXXX- 1221
L RQIVEGL HIHGQGIIHRDLTPNNIFFDARNDIKIGDFG
Sbjct: 571 LFRQIVEGLVHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLDQDPGVPPD 630
Query: 1222 TPGVSVDGTGRVGTYFYTAPEIEQGWLKINEKADMYSFGIVFLELWHPFSTAMERHIVLS 1401
T GVSVD TG+VGTYFYTAPEIEQGW KI+EKADMYS G+VF ELWHPF TAMER IVL+
Sbjct: 631 TTGVSVDRTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGVVFFELWHPFGTAMERSIVLN 690
Query: 1402 DLKQKGELPSDWVAEFPEQAILLRRLMSPSPSDR 1503
DLKQKGELPS WVAEFPEQA LL+ LMSPSPSDR
Sbjct: 691 DLKQKGELPSSWVAEFPEQASLLQHLMSPSPSDR 724
>ref|XP_002533444.1| eif2alpha kinase, putative [Ricinus communis]
gi|223526706|gb|EEF28940.1| eif2alpha kinase, putative
[Ricinus communis]
Length = 1162
Score = 579 bits (1493), Expect = e-163
Identities = 312/518 (60%), Positives = 367/518 (70%), Gaps = 17/518 (3%)
Frame = +1
Query: 1 DGRGHEYGTFEKTTGPDVMPL-IQVTRQGPLRNLSGKAALRTATSKLEILDEKSEDESES 177
DG Y EK PL +Q +QGPL + +KL+ L+E++E++++S
Sbjct: 184 DGSKAGYEVQEKKLDKVTKPLMLQDPKQGPLVSPG---------AKLDTLEEETEEDNKS 234
Query: 178 IGTCTDQS---DESDGDATASEDTIADEHLSETYNDLKSSE--ESVSLASILHDQSSQTV 342
I T + +S + + + E T +E +E + SE E S AS+ H + ++T+
Sbjct: 235 ISTDSSRSLTEESVENEMGGKEVTSTEESGAEDDDAELESEPWELPSSASLGHHEVTRTI 294
Query: 343 ARDLVLAHLLRVVCAPKGPLADALPEITTELYNLGIVSREVQDYALKPAPLFDRTFNHVF 522
+DL++ H+LR+ CA KG ADALP+IT EL NLG+ S D A KP+ +F+ TF+HVF
Sbjct: 295 EKDLIMVHMLRLACASKGVSADALPQITRELCNLGVFSEGACDLACKPSSIFNETFDHVF 354
Query: 523 GQHMVSSKNTNFWMAASDIREQNSSTTPSSRYLNDFEELQPLGHGGFGHVVLCKNKLDGR 702
QHMVSSK + FW SD+ N+S P+SRYLNDFEELQPLGHGGFGHVVLCKNKLDGR
Sbjct: 355 HQHMVSSKVSQFWKPTSDLGGSNTSL-PNSRYLNDFEELQPLGHGGFGHVVLCKNKLDGR 413
Query: 703 QYAMKKIRLKDKSPPLNDRIVREVATLSRLQHQHVVRYYQAWFEAGVFGH-DNGPWGSES 879
QYA+KKIRLKDKS P+NDRI+REVATLSRLQH HVVRYYQAWFE GV G + W +
Sbjct: 414 QYAVKKIRLKDKSLPVNDRILREVATLSRLQHLHVVRYYQAWFETGVVGSFGDTSWDYST 473
Query: 880 ARSSGFSYIDASST---------DFTENDKLESTYLYIQMEYCPRTLRQMFESYDHFDKE 1032
A SS SY ASST D ++ KL+STYLYIQMEYCPRTLRQ+FESY HFDKE
Sbjct: 474 AASSTISYHGASSTISYHGASSADIGQDVKLDSTYLYIQMEYCPRTLRQVFESYKHFDKE 533
Query: 1033 LAWHLSRQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGXXXXXXXXXXXXXXX 1212
L WH RQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFG
Sbjct: 534 LVWHQFRQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGLAKFLKLEQLDHDAT 593
Query: 1213 XXX-TPGVSVDGTGRVGTYFYTAPEIEQGWLKINEKADMYSFGIVFLELWHPFSTAMERH 1389
T GVS DGTG+VGTYFYTAPEIEQGW KI+EK DMYS G+VF ELWHPF TAMERH
Sbjct: 594 LPTDTSGVSADGTGQVGTYFYTAPEIEQGWPKIDEKVDMYSLGVVFFELWHPFGTAMERH 653
Query: 1390 IVLSDLKQKGELPSDWVAEFPEQAILLRRLMSPSPSDR 1503
I+LSDLKQKGELPS WVA+FPEQA LLR+LMSPSPSDR
Sbjct: 654 IILSDLKQKGELPSSWVAQFPEQASLLRQLMSPSPSDR 691
>ref|XP_003540698.1| PREDICTED: probable serine/threonine-protein kinase GCN2-like
[Glycine max]
Length = 1222
Score = 557 bits (1435), Expect = e-156
Identities = 294/469 (62%), Positives = 346/469 (73%), Gaps = 11/469 (2%)
Frame = +1
Query: 130 SKLEILDEKSEDESESIG-TCTDQSDESDGDATASEDT----IADEHLSETYNDLKSSEE 294
+KL+ + E SED + S+ T + +S D +E I DE+ +E + SE
Sbjct: 247 AKLDTVGEVSEDSNNSLSLTSSSRSLVEDFVGNKNEGEKEYFIVDEYTTEHNEGINESES 306
Query: 295 SVSLAS--ILHDQSSQTVARDLVLAHLLRVVCAPKGPLADALPEITTELYNLGIVSREVQ 468
S SL+S + H Q SQTV +DL++ H+LR+VCA KG AD LP++ TEL NLGI+S +
Sbjct: 307 SESLSSESLPHHQPSQTVEKDLIMVHMLRLVCASKGTFADCLPQVVTELCNLGIISDSAR 366
Query: 469 DYALKPAPLFDRTFNHVFGQHMVSSKNTNFWMAASDIREQNSSTTP-SSRYLNDFEELQP 645
D A +P +F++TFN VF +H+ SS+ + FW DI S+T P SRYLNDFEEL+P
Sbjct: 367 DMASEPPSIFNKTFNRVFQKHLASSRISQFWKP--DIG--GSNTVPHGSRYLNDFEELRP 422
Query: 646 LGHGGFGHVVLCKNKLDGRQYAMKKIRLKDKSPPLNDRIVREVATLSRLQHQHVVRYYQA 825
LGHGGFGHVVLCKNKLDGRQYA+KKIRLKDKS P DRI+REVATLSRLQHQHVVRYYQA
Sbjct: 423 LGHGGFGHVVLCKNKLDGRQYAVKKIRLKDKSMP--DRILREVATLSRLQHQHVVRYYQA 480
Query: 826 WFEAGVF-GHDNGPWGSESARSSGFSYIDASSTD-FTENDKLESTYLYIQMEYCPRTLRQ 999
WFE GV + + WGS++ SS FSY A+S D ++LESTYLYIQMEYCPRTLRQ
Sbjct: 481 WFETGVSDSYGDSTWGSKTTVSSTFSYKAATSNDALGHENQLESTYLYIQMEYCPRTLRQ 540
Query: 1000 MFESYDHFDKELAWHLSRQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGXXXX 1179
+FESY+HFDKELAWHL RQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFG
Sbjct: 541 VFESYNHFDKELAWHLFRQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFGLAKF 600
Query: 1180 XXXXXXXXXXXXXX-TPGVSVDGTGRVGTYFYTAPEIEQGWLKINEKADMYSFGIVFLEL 1356
GVS+DGTG+VGTYFYTAPEIEQGW KI+EKADMYS G+VF EL
Sbjct: 601 LKLEQLDQDLGHPADATGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGVVFFEL 660
Query: 1357 WHPFSTAMERHIVLSDLKQKGELPSDWVAEFPEQAILLRRLMSPSPSDR 1503
WHPF T MERH++LSDLKQK E+P WV EFPEQ LLR+LMSP+PSDR
Sbjct: 661 WHPFGTGMERHVILSDLKQKREVPHTWVVEFPEQESLLRQLMSPAPSDR 709
>ref|XP_002876502.1| kinase family protein [Arabidopsis lyrata subsp. lyrata]
gi|297322340|gb|EFH52761.1| kinase family protein
[Arabidopsis lyrata subsp. lyrata]
Length = 1242
Score = 555 bits (1431), Expect = e-156
Identities = 295/473 (62%), Positives = 338/473 (71%), Gaps = 9/473 (1%)
Frame = +1
Query: 112 ALRTATSKLEILDEKSEDESESIGTCTDQSDESDGDATASEDTIADEHLSE-TYNDLKSS 288
AL T+KL + E + D++ SI + D ++ ++ + +L + T D S+
Sbjct: 250 ALPAPTAKLNTVQEDNVDDT-SISSFDSSKSTDDVESGLFQNEKKESNLQDDTAEDDSSN 308
Query: 289 EESVSLAS-----ILHDQSSQTVARDLVLAHLLRVVCAPKGPLADALPEITTELYNLGIV 453
ES SL S + DQ Q +DL++ HLLRV C +GPLADALP+IT EL+ LGI+
Sbjct: 309 SESESLGSWSSDSLSQDQVPQISKKDLLMVHLLRVACTSRGPLADALPQITDELHQLGIL 368
Query: 454 SREVQDYALKPAPLFDRTFNHVFGQHMVSSKNTNFWMAASDIREQNSSTTPSSRYLNDFE 633
S E D A K +P F+RTF H F Q+MVS+ FW SD E N+S PSSRYLNDFE
Sbjct: 369 SEEALDLASKSSPDFNRTFEHAFNQNMVSTSVPQFWEPPSDSGEPNASL-PSSRYLNDFE 427
Query: 634 ELQPLGHGGFGHVVLCKNKLDGRQYAMKKIRLKDKSPPLNDRIVREVATLSRLQHQHVVR 813
EL+PLG GGFGHVVLCKNKLDGRQYA+KKIRLK+K P+N RIVREVATLSRLQHQHVVR
Sbjct: 428 ELKPLGQGGFGHVVLCKNKLDGRQYAVKKIRLKEKEIPVNSRIVREVATLSRLQHQHVVR 487
Query: 814 YYQAWFEAGVFGHDNGP-WGSESARSSGFSYIDASSTDFTENDK-LESTYLYIQMEYCPR 987
YYQAWFE GV G WGS++A SS FSY A ST+ E D LESTYLYIQMEYCPR
Sbjct: 488 YYQAWFETGVVDPFAGANWGSKTAGSSMFSYSGAVSTEIPEQDNNLESTYLYIQMEYCPR 547
Query: 988 TLRQMFESYDHFDKELAWHLSRQIVEGLAHIHGQGIIHRDLTPNNIFFDARNDIKIGDFG 1167
TLRQ+FESY+HFDK+ AWHL RQIVEGLAHIHGQGIIHRD TPNNIFFDARNDIKIGDFG
Sbjct: 548 TLRQVFESYNHFDKDFAWHLIRQIVEGLAHIHGQGIIHRDFTPNNIFFDARNDIKIGDFG 607
Query: 1168 XXXXXXXXXXXXXXXXXX-TPGVSVDGTGRVGTYFYTAPEIEQGWLKINEKADMYSFGIV 1344
G VD TG+ GTYFYTAPEIEQ W KI+EKADMYS G+V
Sbjct: 608 LAKFLKLEQLDQDGGFSTDVAGSGVDSTGQAGTYFYTAPEIEQDWPKIDEKADMYSLGVV 667
Query: 1345 FLELWHPFSTAMERHIVLSDLKQKGELPSDWVAEFPEQAILLRRLMSPSPSDR 1503
F ELWHPF TAMERH++L+DLK KGELP WV EFPEQA LLRRLMSPSPSDR
Sbjct: 668 FFELWHPFGTAMERHVILTDLKLKGELPLKWVNEFPEQASLLRRLMSPSPSDR 720
>ref|NP_191500.2| non-specific serine/threonine protein kinase [Arabidopsis thaliana]
gi|68052253|sp|Q9LX30.2|GCN2_ARATH RecName: Full=Probable
serine/threonine-protein kinase GCN2
gi|24940154|emb|CAD30860.1| GCN2 homologue [Arabidopsis
thaliana] gi|332646397|gb|AEE79918.1| non-specific
serine/threonine protein kinase [Arabidopsis thaliana]
Length = 1241
Score = 553 bits (1425), Expect = e-155
Identities = 297/495 (60%), Positives = 346/495 (69%), Gaps = 16/495 (3%)
Frame = +1
Query: 67 QVTRQGPLRNLSG-------KAALRTATSKLEILDEKSEDESESIGTCTDQSDESDGDAT 225
++ Q P +NL + AL +KL + E++ D++ SI + D ++
Sbjct: 227 RILHQKPDKNLKRFEDHAKEEVALPAPIAKLNTVQEENVDDT-SISSFDSSKSTDDVESG 285
Query: 226 ASEDTIADEHLSE-TYNDLKSSEESVSLAS-----ILHDQSSQTVARDLVLAHLLRVVCA 387
++ + +L + T D ++ ES SL S + DQ Q +DL++ HLLRV C
Sbjct: 286 LFQNEKKESNLQDDTAEDDSTNSESESLGSWSSDSLAQDQVPQISKKDLLMVHLLRVACT 345
Query: 388 PKGPLADALPEITTELYNLGIVSREVQDYALKPAPLFDRTFNHVFGQHMVSSKNTNFWMA 567
+GPLADALP+IT EL+ LGI+S EV D A K +P F+RTF H F Q+M S+ FW
Sbjct: 346 SRGPLADALPQITDELHELGILSEEVLDLASKSSPDFNRTFEHAFNQNMASTSVPQFWEP 405
Query: 568 ASDIREQNSSTTPSSRYLNDFEELQPLGHGGFGHVVLCKNKLDGRQYAMKKIRLKDKSPP 747
SD E N+S PSSRYLNDFEEL+PLG GGFGHVVLCKNKLDGRQYA+KKIRLKDK P
Sbjct: 406 PSDSCEPNASL-PSSRYLNDFEELKPLGQGGFGHVVLCKNKLDGRQYAVKKIRLKDKEIP 464
Query: 748 LNDRIVREVATLSRLQHQHVVRYYQAWFEAGVFGHDNGP-WGSESARSSGFSYIDASSTD 924
+N RIVREVATLSRLQHQHVVRYYQAWFE GV G WGS++A SS FSY A ST+
Sbjct: 465 VNSRIVREVATLSRLQHQHVVRYYQAWFETGVVDPFAGANWGSKTAGSSMFSYSGAVSTE 524
Query: 925 FTEND-KLESTYLYIQMEYCPRTLRQMFESYDHFDKELAWHLSRQIVEGLAHIHGQGIIH 1101
E D LESTYLYIQMEYCPRTLRQ+FESY+HFDK+ AWHL RQIVEGLAHIHGQGIIH
Sbjct: 525 IPEQDNNLESTYLYIQMEYCPRTLRQVFESYNHFDKDFAWHLIRQIVEGLAHIHGQGIIH 584
Query: 1102 RDLTPNNIFFDARNDIKIGDFG-XXXXXXXXXXXXXXXXXXTPGVSVDGTGRVGTYFYTA 1278
RD TPNNIFFDARNDIKIGDFG G VD TG+ GTYFYTA
Sbjct: 585 RDFTPNNIFFDARNDIKIGDFGLAKFLKLEQLDQDGGFSTDVAGSGVDSTGQAGTYFYTA 644
Query: 1279 PEIEQGWLKINEKADMYSFGIVFLELWHPFSTAMERHIVLSDLKQKGELPSDWVAEFPEQ 1458
PEIEQ W KI+EKADMYS G+VF ELWHPF TAMERH++L++LK KGELP WV EFPEQ
Sbjct: 645 PEIEQDWPKIDEKADMYSLGVVFFELWHPFGTAMERHVILTNLKLKGELPLKWVNEFPEQ 704
Query: 1459 AILLRRLMSPSPSDR 1503
A LLRRLMSPSPSDR
Sbjct: 705 ASLLRRLMSPSPSDR 719