BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00027487
         (1204 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004160432.1| PREDICTED: probable protein phosphatase 2C 8...   330   e-120
ref|XP_004137326.1| PREDICTED: probable protein phosphatase 2C 8...   328   e-119
ref|XP_002277457.1| PREDICTED: probable protein phosphatase 2C 8...   333   e-119
ref|XP_003620354.1| hypothetical protein MTR_6g081850 [Medicago ...   326   e-116
ref|NP_564045.1| putative protein phosphatase 2C 8 [Arabidopsis ...   311   e-114

>ref|XP_004160432.1| PREDICTED: probable protein phosphatase 2C 8-like [Cucumis sativus]
          Length = 348

 Score =  330 bits (846), Expect(2) = e-120
 Identities = 161/207 (77%), Positives = 179/207 (86%)
 Frame = +1

Query: 394  LDVKAAKRAIFDGFRKTDESLLQESAAGGWMDGATAVCVWVLGQTVFVANIGDAKAVIAR 573
            LDVKA K+AI DGFRKTDESLLQES+AGGW DGATAVCVWVLGQTVFVAN+GDAKAV+AR
Sbjct: 140  LDVKATKKAILDGFRKTDESLLQESSAGGWQDGATAVCVWVLGQTVFVANVGDAKAVVAR 199

Query: 574  XXXXXXXXXXXXXXXLLKAIVLTREHKAIYPQERIRIQKAGGTVSSNGRLQGRLEVSRAF 753
                            LKAIVLTREHKAIYPQER RIQKAGG V SNGRLQGRLEVSRAF
Sbjct: 200  SLTTDKSTTNSNGGSSLKAIVLTREHKAIYPQERARIQKAGGVVGSNGRLQGRLEVSRAF 259

Query: 754  GDRHFKKVGVVATPDIHSFSLTERDKFIILGCDGLWGVFGASDAVEFVHKLLKEGLSVAA 933
            GDR FKK+GV+ATPDIHSF LT+R+ FIILGCDGLWGVFG SDAV+FV KLLK+GLSVA+
Sbjct: 260  GDRQFKKLGVIATPDIHSFELTDREHFIILGCDGLWGVFGPSDAVDFVQKLLKDGLSVAS 319

Query: 934  VTRRLVREAVRERHCKDNCTAVVIIFK 1014
            ++RRLVREA+RER CKDNCTA+V++F+
Sbjct: 320  ISRRLVREAIRERRCKDNCTAIVVVFR 346



 Score =  128 bits (321), Expect(2) = e-120
 Identities = 66/133 (49%), Positives = 84/133 (63%), Gaps = 15/133 (11%)
 Frame = +3

Query: 39  SAKQMEGKSNSSISNPKKRDSDFNSSDSIVKKSKADCKDDENTDQNM------------- 179
           +AK +  +  S +    KR +D   +D  VKK K     DE   +N+             
Sbjct: 4   TAKSILEEETSKLEPKSKRGADVEDADVTVKKFKTQAAADETGARNLEAVNGDEELKVIK 63

Query: 180 --YNFVIEADAAEDKGSRHAMEDKCVVIPDASFDFPGTLRCAHYAVYDGHGGQLAAEYAQ 353
               FVIEADAAEDKGSRH MED  V++ DAS DFPG LRCAH+A+YDGHGG+LAAE+AQ
Sbjct: 64  RDVEFVIEADAAEDKGSRHTMEDAWVLLLDASLDFPGKLRCAHFAIYDGHGGRLAAEHAQ 123

Query: 354 RHLHQNILSAGLP 392
           +HL +N+LS+GLP
Sbjct: 124 KHLQKNVLSSGLP 136


>ref|XP_004137326.1| PREDICTED: probable protein phosphatase 2C 8-like [Cucumis sativus]
          Length = 348

 Score =  328 bits (842), Expect(2) = e-119
 Identities = 160/207 (77%), Positives = 178/207 (85%)
 Frame = +1

Query: 394  LDVKAAKRAIFDGFRKTDESLLQESAAGGWMDGATAVCVWVLGQTVFVANIGDAKAVIAR 573
            LDVKA K+AI DGFRKTDESLLQES+AGGW DGATAVCVWVLGQTVFVAN+GDAKAV+AR
Sbjct: 140  LDVKATKKAILDGFRKTDESLLQESSAGGWQDGATAVCVWVLGQTVFVANVGDAKAVVAR 199

Query: 574  XXXXXXXXXXXXXXXLLKAIVLTREHKAIYPQERIRIQKAGGTVSSNGRLQGRLEVSRAF 753
                            LKAIVLTREHKAIYPQER RIQKAGG V SNGRLQGRLEVSRAF
Sbjct: 200  SLTTDKSTTNSNGGSSLKAIVLTREHKAIYPQERARIQKAGGVVGSNGRLQGRLEVSRAF 259

Query: 754  GDRHFKKVGVVATPDIHSFSLTERDKFIILGCDGLWGVFGASDAVEFVHKLLKEGLSVAA 933
            GDR FKK+GV+ TPDIHSF LT+R+ FIILGCDGLWGVFG SDAV+FV KLLK+GLSVA+
Sbjct: 260  GDRQFKKLGVIVTPDIHSFELTDREHFIILGCDGLWGVFGPSDAVDFVQKLLKDGLSVAS 319

Query: 934  VTRRLVREAVRERHCKDNCTAVVIIFK 1014
            ++RRLVREA+RER CKDNCTA+V++F+
Sbjct: 320  ISRRLVREAIRERRCKDNCTAIVVVFR 346



 Score =  128 bits (321), Expect(2) = e-119
 Identities = 66/133 (49%), Positives = 84/133 (63%), Gaps = 15/133 (11%)
 Frame = +3

Query: 39  SAKQMEGKSNSSISNPKKRDSDFNSSDSIVKKSKADCKDDENTDQNM------------- 179
           +AK +  +  S +    KR +D   +D  VKK K     DE   +N+             
Sbjct: 4   TAKSILEEETSKLEPKSKRGADVEDADVTVKKFKTQAAADETGARNLEAVNGDEELKVIK 63

Query: 180 --YNFVIEADAAEDKGSRHAMEDKCVVIPDASFDFPGTLRCAHYAVYDGHGGQLAAEYAQ 353
               FVIEADAAEDKGSRH MED  V++ DAS DFPG LRCAH+A+YDGHGG+LAAE+AQ
Sbjct: 64  RDVEFVIEADAAEDKGSRHTMEDAWVLLLDASLDFPGKLRCAHFAIYDGHGGRLAAEHAQ 123

Query: 354 RHLHQNILSAGLP 392
           +HL +N+LS+GLP
Sbjct: 124 KHLQKNVLSSGLP 136


>ref|XP_002277457.1| PREDICTED: probable protein phosphatase 2C 8 [Vitis vinifera]
            gi|297734261|emb|CBI15508.3| unnamed protein product
            [Vitis vinifera]
          Length = 378

 Score =  333 bits (853), Expect(2) = e-119
 Identities = 165/207 (79%), Positives = 179/207 (86%)
 Frame = +1

Query: 394  LDVKAAKRAIFDGFRKTDESLLQESAAGGWMDGATAVCVWVLGQTVFVANIGDAKAVIAR 573
            LDVKAAK+AI DGFR+TDESLLQES AGGW DG+TAVCVW+LGQ VFVANIGDAKAV+AR
Sbjct: 170  LDVKAAKKAILDGFRRTDESLLQESLAGGWQDGSTAVCVWILGQKVFVANIGDAKAVVAR 229

Query: 574  XXXXXXXXXXXXXXXLLKAIVLTREHKAIYPQERIRIQKAGGTVSSNGRLQGRLEVSRAF 753
                            LKAIVLTREHKAIYPQER RIQKAGG+VSSNGRLQGRLEVSRAF
Sbjct: 230  SSTTDGSENQSDGVSQLKAIVLTREHKAIYPQERARIQKAGGSVSSNGRLQGRLEVSRAF 289

Query: 754  GDRHFKKVGVVATPDIHSFSLTERDKFIILGCDGLWGVFGASDAVEFVHKLLKEGLSVAA 933
            GDR FKKVGVVATPD+HSF LTER+ FIILGCDGLWGVFG SDAV+FVHK+LKEGL VA 
Sbjct: 290  GDRQFKKVGVVATPDVHSFDLTEREHFIILGCDGLWGVFGPSDAVDFVHKMLKEGLPVAT 349

Query: 934  VTRRLVREAVRERHCKDNCTAVVIIFK 1014
            V+RRLVREAVRER CKDNCTA+VI+F+
Sbjct: 350  VSRRLVREAVRERRCKDNCTAIVIVFR 376



 Score =  122 bits (306), Expect(2) = e-119
 Identities = 59/102 (57%), Positives = 78/102 (76%)
 Frame = +3

Query: 87  KKRDSDFNSSDSIVKKSKADCKDDENTDQNMYNFVIEADAAEDKGSRHAMEDKCVVIPDA 266
           K   S+  ++DS V +     ++ ++  +   +F IEADAAEDKGSRH+MED  VV+PDA
Sbjct: 67  KPEVSNQGNNDSQVCEQNGCSQEKQSARKE--HFEIEADAAEDKGSRHSMEDAWVVLPDA 124

Query: 267 SFDFPGTLRCAHYAVYDGHGGQLAAEYAQRHLHQNILSAGLP 392
           S +FPG LRCAH+A+YDGHGG+LAA+YAQ+HLH N+LSAGLP
Sbjct: 125 SLEFPGKLRCAHFAIYDGHGGRLAAQYAQKHLHANVLSAGLP 166


>ref|XP_003620354.1| hypothetical protein MTR_6g081850 [Medicago truncatula]
            gi|355495369|gb|AES76572.1| hypothetical protein
            MTR_6g081850 [Medicago truncatula]
          Length = 321

 Score =  326 bits (835), Expect(2) = e-116
 Identities = 162/208 (77%), Positives = 176/208 (84%)
 Frame = +1

Query: 394  LDVKAAKRAIFDGFRKTDESLLQESAAGGWMDGATAVCVWVLGQTVFVANIGDAKAVIAR 573
            LDVK AKR+I DGFRKTDESLLQ SA GGW DGATAVCVWVLGQ VF+AN+GDAKAV+AR
Sbjct: 113  LDVKTAKRSILDGFRKTDESLLQASAEGGWQDGATAVCVWVLGQKVFIANVGDAKAVLAR 172

Query: 574  XXXXXXXXXXXXXXXLLKAIVLTREHKAIYPQERIRIQKAGGTVSSNGRLQGRLEVSRAF 753
                            LKAIVLTREHK I+PQER RIQKAGG VSSNGRLQGRLEVSRAF
Sbjct: 173  STIPDGSKDNSDGVPALKAIVLTREHKPIFPQERARIQKAGGIVSSNGRLQGRLEVSRAF 232

Query: 754  GDRHFKKVGVVATPDIHSFSLTERDKFIILGCDGLWGVFGASDAVEFVHKLLKEGLSVAA 933
            GDR FKKVG+VATPDIHSF LT+RD+FIILGCDGLWGVFG SDAV+FV KLLKEGLSV  
Sbjct: 233  GDRQFKKVGLVATPDIHSFDLTDRDRFIILGCDGLWGVFGPSDAVDFVQKLLKEGLSVTT 292

Query: 934  VTRRLVREAVRERHCKDNCTAVVIIFKR 1017
            V+RRLV+EAVRER CKDNCTA+VI+FK+
Sbjct: 293  VSRRLVKEAVRERRCKDNCTAIVIVFKQ 320



 Score =  120 bits (300), Expect(2) = e-116
 Identities = 56/101 (55%), Positives = 71/101 (70%)
 Frame = +3

Query: 90  KRDSDFNSSDSIVKKSKADCKDDENTDQNMYNFVIEADAAEDKGSRHAMEDKCVVIPDAS 269
           KR+SD  + D I    K   +  +        F+IEADAAEDKG R  MED+ VV+ DA 
Sbjct: 9   KRESDSENKDEISASKKLRVESSKENGSKEVTFLIEADAAEDKGLRQTMEDEWVVLIDAV 68

Query: 270 FDFPGTLRCAHYAVYDGHGGQLAAEYAQRHLHQNILSAGLP 392
            D+PG LRCAH+A+YDGHGG+LAAEYAQ+HLH N+L++GLP
Sbjct: 69  KDYPGNLRCAHFAIYDGHGGRLAAEYAQKHLHGNVLASGLP 109


>ref|NP_564045.1| putative protein phosphatase 2C 8 [Arabidopsis thaliana]
            gi|226739232|sp|Q9LMT1.2|P2C08_ARATH RecName:
            Full=Probable protein phosphatase 2C 8; Short=AtPP2C08
            gi|109946401|gb|ABG48379.1| At1g18030 [Arabidopsis
            thaliana] gi|332191544|gb|AEE29665.1| putative protein
            phosphatase 2C 8 [Arabidopsis thaliana]
          Length = 351

 Score =  311 bits (798), Expect(2) = e-114
 Identities = 155/208 (74%), Positives = 172/208 (82%)
 Frame = +1

Query: 394  LDVKAAKRAIFDGFRKTDESLLQESAAGGWMDGATAVCVWVLGQTVFVANIGDAKAVIAR 573
            LDVK AK+AI +GFRKTDE LLQ+S +GGW DGATAVCVW+L Q VFVANIGDAKAV+AR
Sbjct: 143  LDVKVAKKAILEGFRKTDELLLQKSVSGGWQDGATAVCVWILDQKVFVANIGDAKAVLAR 202

Query: 574  XXXXXXXXXXXXXXXLLKAIVLTREHKAIYPQERIRIQKAGGTVSSNGRLQGRLEVSRAF 753
                            LKAIVLTREHKAIYPQER RIQK+GG +SSNGRLQGRLEVSRAF
Sbjct: 203  SSTTNELGNHTEAGNPLKAIVLTREHKAIYPQERSRIQKSGGVISSNGRLQGRLEVSRAF 262

Query: 754  GDRHFKKVGVVATPDIHSFSLTERDKFIILGCDGLWGVFGASDAVEFVHKLLKEGLSVAA 933
            GDRHFKK GV ATPDIH+F LTER+ F+ILGCDGLW VFG SDAV FV KLLKEGL V+ 
Sbjct: 263  GDRHFKKFGVSATPDIHAFELTERENFMILGCDGLWEVFGPSDAVGFVQKLLKEGLHVST 322

Query: 934  VTRRLVREAVRERHCKDNCTAVVIIFKR 1017
            V+RRLV+EAV+ER CKDNCTA+VI+FKR
Sbjct: 323  VSRRLVKEAVKERRCKDNCTAIVIVFKR 350



 Score =  127 bits (318), Expect(2) = e-114
 Identities = 60/126 (47%), Positives = 84/126 (66%), Gaps = 3/126 (2%)
 Frame = +3

Query: 24  PKNKKSAKQMEGKSN-SSISNPKKRDSDFNSSDSIVKKSKADCKDDENT--DQNMYNFVI 194
           P NK +A     +   S +   KK +      +++      + ++D+ +   +    F++
Sbjct: 14  PNNKHAADSFSSEDLVSPVKKAKKSEEVSGGGEAVAAVGNREAEEDKPSFVSEEKKEFLV 73

Query: 195 EADAAEDKGSRHAMEDKCVVIPDASFDFPGTLRCAHYAVYDGHGGQLAAEYAQRHLHQNI 374
           EAD AEDKG+RH MED  VV+PDAS DFPGTLRCAH+A+YDGHGG+LAAE+A++HLH N+
Sbjct: 74  EADVAEDKGARHTMEDVWVVLPDASLDFPGTLRCAHFAIYDGHGGRLAAEFAKKHLHLNV 133

Query: 375 LSAGLP 392
           LSAGLP
Sbjct: 134 LSAGLP 139