BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00027279
         (1822 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABD33126.2| RNA-directed DNA polymerase (Reverse transcriptas...   394   e-119
ref|XP_003635424.1| PREDICTED: LOW QUALITY PROTEIN: putative rib...   343   e-118
emb|CCA66040.1| hypothetical protein [Beta vulgaris subsp. vulga...   353   e-114
emb|CCA66050.1| hypothetical protein [Beta vulgaris subsp. vulga...   330   e-108
pir||T00833 RNA-directed DNA polymerase homolog T13L16.7 - Arabi...   331   e-106

>gb|ABD33126.2| RNA-directed DNA polymerase (Reverse transcriptase) [Medicago
            truncatula]
          Length = 653

 Score =  394 bits (1013), Expect(2) = e-119
 Identities = 188/409 (45%), Positives = 266/409 (65%)
 Frame = -1

Query: 1822 GNEGEVALKLDISKAYDRVSWRYLRHRLKVMGFCNTWASWMMMCVKSVSYNFYLNGSSIG 1643
            G +GE ALK+DISK YDRV W YL   ++ MGF   W  WM M V  VSY+  +N   +G
Sbjct: 158  GIKGEAALKIDISKTYDRVDWNYLFSIMEKMGFNPIWIKWMRMSVTMVSYHVLVNDDRVG 217

Query: 1642 PIVPKRGLRQGDPLSPYLFLLCVEGLSSAIDQANREGSLHGCCISPTAPTISHLLFADDS 1463
            PI P RGLRQGDPLSPYLF+LC EG+S+ I QA R   LHG  +   APT+SHLLFADDS
Sbjct: 218  PIYPGRGLRQGDPLSPYLFILCAEGMSTLIKQAERNNILHGVKVCKRAPTVSHLLFADDS 277

Query: 1462 FLFFRGTVEEANNIRALLFNYARCSGQSVNFQKSGVFFSANVRRDKQQQISSILGVFNDI 1283
            FLFFR    E   ++ +L  YA  S Q +N QKS ++FS NV   K+  +S++L V   I
Sbjct: 278  FLFFRANENETRALKDILDTYANASDQLINMQKSEIYFSRNVPVTKKNTLSNMLWVSEGI 337

Query: 1282 SSSKYLGLPSLVGRSKKRVFGYLKERVSKRIQSWQSKSISRAGKTVLIRNVAQAIPAFTM 1103
               KYLGLPS++GRSKK +F Y+K+R+  RI    SK +S+AGK VLI++VAQAIP++ M
Sbjct: 338  GIRKYLGLPSMIGRSKKSIFNYIKDRIWNRISGLSSKMLSQAGKEVLIKSVAQAIPSYCM 397

Query: 1102 SCFLLPKSLCQEIERKFNNYWWSSGKAGSKGLNWLSWKNMGLPKKKGGLGFRDLYGFNIA 923
            S FLLP S+  +IE+  N++WW     G  G+ W+SW+ + + K+ GG+GFR ++GF++A
Sbjct: 398  SVFLLPHSIADDIEKMLNSFWWGMKNEGRGGIRWMSWERLTMRKEWGGMGFRHIHGFDLA 457

Query: 922  LLGKHCWNFMSNPLSLVARLFKARYYPSVHVLKATAGTGASFIWKGIWTAKEELKKGFRW 743
            + GK CWNF++NP ++V+R+FKA+Y+ + + L A+ G   S++W+ IW+++  LK G++W
Sbjct: 458  MSGKQCWNFLTNPDAMVSRIFKAKYFTNENFLGASLGHNPSYVWRSIWSSRIFLKTGYQW 517

Query: 742  VLGNGNDIVATKDLWLRNKKDYGVEQSHFYEGRSELVSSLINSNSKSWN 596
             +G+G+                           +  VS LI++ ++SWN
Sbjct: 518  RIGDGS--------------------------TTATVSHLIDAQTRSWN 540



 Score = 64.3 bits (155), Expect(2) = e-119
 Identities = 30/83 (36%), Positives = 45/83 (54%)
 Frame = -3

Query: 587 NDRVAWTGTSNGVYSAKSGYQFWFDRMVGTGDVPQNQGWSKVWKLDIPHKVKIFVWRFCR 408
           ND + W     GVYS K+ Y+   D ++   +       +K+W L IP KVK F+WR  R
Sbjct: 567 NDAIIWRFDKKGVYSVKNAYRVCVDVLINRDEWKVEGDRNKLWSLPIPPKVKHFMWRLGR 626

Query: 407 NNVPVRMRLSSKGISLPLSCPMC 339
           + +P R RL+ KG+    +C +C
Sbjct: 627 DCLPNRQRLNCKGVVCTSNCVIC 649


>ref|XP_003635424.1| PREDICTED: LOW QUALITY PROTEIN: putative ribonuclease H protein
            At1g65750-like [Vitis vinifera]
          Length = 820

 Score =  343 bits (880), Expect(2) = e-118
 Identities = 166/376 (44%), Positives = 232/376 (61%)
 Frame = -1

Query: 1822 GNEGEVALKLDISKAYDRVSWRYLRHRLKVMGFCNTWASWMMMCVKSVSYNFYLNGSSIG 1643
            G  G  A K+DISKAYD++ W YL   L+ MGF + W  WM M V +VSY     G  +G
Sbjct: 34   GRIGYAAFKIDISKAYDKLEWNYLLSVLEAMGFSSKWLEWMRMRVCTVSYKVVFGGELLG 93

Query: 1642 PIVPKRGLRQGDPLSPYLFLLCVEGLSSAIDQANREGSLHGCCISPTAPTISHLLFADDS 1463
            PI P RGLRQ DPLSPYLF+L  EGLS+ + Q  R G LHGC ++  A T+ HL FADD 
Sbjct: 94   PIYPTRGLRQEDPLSPYLFILAAEGLSALLKQGERCGVLHGCSVARGASTVLHLFFADDL 153

Query: 1462 FLFFRGTVEEANNIRALLFNYARCSGQSVNFQKSGVFFSANVRRDKQQQISSILGVFNDI 1283
            +LFF+ T  E+ +++ +L  Y   SGQ +N  KS + FS N     ++ I SIL V    
Sbjct: 154  YLFFKATESESRSLKQILLRYQNLSGQEINLNKSALTFSRNTDDVVKRGICSILQVEEXA 213

Query: 1282 SSSKYLGLPSLVGRSKKRVFGYLKERVSKRIQSWQSKSISRAGKTVLIRNVAQAIPAFTM 1103
                YLG+P++VG++K+++F +++ +V  RIQ+W  + +SRAGK + ++ VAQ+IP + M
Sbjct: 214  DPGIYLGMPAVVGKNKRQLFEFVRRKVWNRIQNWNGRRLSRAGKEICLKTVAQSIPTYVM 273

Query: 1102 SCFLLPKSLCQEIERKFNNYWWSSGKAGSKGLNWLSWKNMGLPKKKGGLGFRDLYGFNIA 923
               LLPK LC++IE   N ++W S     + + W+SW  M   KK GGLGFR L  FN+A
Sbjct: 274  QLLLLPKDLCRDIESMMNGFFWDSNPQ-CRSIRWMSWGKMCKQKKDGGLGFRKLXDFNLA 332

Query: 922  LLGKHCWNFMSNPLSLVARLFKARYYPSVHVLKATAGTGASFIWKGIWTAKEELKKGFRW 743
            LL K  W F+ NP SLV R+F+ARY+ +   L A  G+  S++W+ I  A+  LK+G  W
Sbjct: 333  LLAKQGWRFLRNPDSLVTRIFQARYFRNSSFLNAELGSNPSYMWRSILAAQGLLKRGCYW 392

Query: 742  VLGNGNDIVATKDLWL 695
             + +G  +    D WL
Sbjct: 393  SIASGTKVQVWGDSWL 408



 Score =  111 bits (277), Expect(2) = e-118
 Identities = 61/222 (27%), Positives = 99/222 (44%), Gaps = 4/222 (1%)
 Frame = -3

Query: 686  EGLWGGAESFL*GSF*VGXXXXXXXXXXXXXEVNDRVAWTGTSNGVYSAKSGYQ----FW 519
            EGLW   E F+   F                   D+++W+  + G Y+A+SGY     F 
Sbjct: 435  EGLW--REDFIRDKFMARDADLILSIPLPMSSREDQISWSFDARGEYTARSGYGALRCFR 492

Query: 518  FDRMVGTGDVPQNQGWSKVWKLDIPHKVKIFVWRFCRNNVPVRMRLSSKGISLPLSCPMC 339
                +   DV  N  W+++WK+  P K+  F WR  RN +P R  L+ + +  P+ CP+C
Sbjct: 493  QSTALVASDVDSNFVWAQLWKVTAPPKILNFAWRAARNCLPTRFALTIRHVDTPMCCPIC 552

Query: 338  DLDIEDMLHLFFKCDFAMECWNHVNLFYDMSNEVSATEWLLNKLSEASSDELVKICTVIW 159
              ++E  LH   +C  A + W+   L     N  S  +WL    +        K   V W
Sbjct: 553  RSELETTLHALVECVAARDVWDESGLAMLQGNFGSFVDWLATMFAYCDFVVFAKYLAVCW 612

Query: 158  GLWFWRNKKVWDNKAVPASFAMDESFRSISSWREARKKVKSS 33
            GLW+ RN  VW+ +   +   ++  F  + SW  A + + ++
Sbjct: 613  GLWWRRNDVVWNGRIWHSQQVVNGCFTMLESWFHANETLATA 654


>emb|CCA66040.1| hypothetical protein [Beta vulgaris subsp. vulgaris]
          Length = 1362

 Score =  353 bits (907), Expect(2) = e-114
 Identities = 177/406 (43%), Positives = 244/406 (60%)
 Frame = -1

Query: 1813 GEVALKLDISKAYDRVSWRYLRHRLKVMGFCNTWASWMMMCVKSVSYNFYLNGSSIGPIV 1634
            G  ALKLD+SKAYDRV W +L   +K MGFC+ W   +M C+ SVS+ F +NG   G + 
Sbjct: 572  GVCALKLDMSKAYDRVEWCFLERVMKKMGFCDGWIDRVMACISSVSFTFNVNGVVEGSLS 631

Query: 1633 PKRGLRQGDPLSPYLFLLCVEGLSSAIDQANREGSLHGCCISPTAPTISHLLFADDSFLF 1454
            P RGLRQGDP+SPYLFLLC +  S+ + +A  E  +HG  I   AP +SHL FADDS LF
Sbjct: 632  PSRGLRQGDPISPYLFLLCADAFSTLLSKAASEKKIHGAQICRGAPVVSHLFFADDSILF 691

Query: 1453 FRGTVEEANNIRALLFNYARCSGQSVNFQKSGVFFSANVRRDKQQQISSILGVFNDISSS 1274
             + +V+E + +  ++  Y R SGQ VN  K+ V FS +V R+++  I ++LGV       
Sbjct: 692  TKASVQECSMVADIISKYERASGQQVNLSKTEVVFSRSVDRERRSAIVNVLGVKEVDRQE 751

Query: 1273 KYLGLPSLVGRSKKRVFGYLKERVSKRIQSWQSKSISRAGKTVLIRNVAQAIPAFTMSCF 1094
            KYLGLP+++GRSKK  F  +KER+ K++Q W+ K +SR GK VLI++VAQAIP + MS F
Sbjct: 752  KYLGLPTIIGRSKKVTFACIKERIWKKLQGWKEKLLSRPGKEVLIKSVAQAIPTYMMSVF 811

Query: 1093 LLPKSLCQEIERKFNNYWWSSGKAGSKGLNWLSWKNMGLPKKKGGLGFRDLYGFNIALLG 914
             LP  L  EI      +WW S     K ++W SW  +  PK  GGLGFRDL+ FN +LL 
Sbjct: 812  SLPSGLIDEIHSLLARFWWGSSDTNRK-MHWHSWDTLCYPKSMGGLGFRDLHCFNQSLLA 870

Query: 913  KHCWNFMSNPLSLVARLFKARYYPSVHVLKATAGTGASFIWKGIWTAKEELKKGFRWVLG 734
            K  W   +   +L+ RL +ARY+ S  +L+A  G   SF W+ IW +K  L +G +W +G
Sbjct: 871  KQAWRLCTGDQTLLYRLLQARYFKSSELLEARRGYNPSFTWRSIWGSKSLLLEGLKWCVG 930

Query: 733  NGNDIVATKDLWLRNKKDYGVEQSHFYEGRSELVSSLINSNSKSWN 596
            +G  I   +D W+  +  + V            V  LI+    +WN
Sbjct: 931  SGERIRVWEDAWILGEGAHMVPTPQADSNLDLKVCDLIDVARGAWN 976



 Score = 87.4 bits (215), Expect(2) = e-114
 Identities = 52/188 (27%), Positives = 90/188 (47%), Gaps = 11/188 (5%)
 Frame = -3

Query: 587  NDRVAWTGTSNGVYSAKSGYQFWFDRM--VGTGDVPQNQG----WSKVWKLDIPHKVKIF 426
            +D   W  + NG++S +S Y  W  R+  V T  +   +     W +VW+L  P K+  F
Sbjct: 1003 DDHRYWWPSRNGIFSVRSCY--WLGRLGPVRTWQLQHGERETELWRRVWQLQGPPKLSHF 1060

Query: 425  VWRFCRNNVPVRMRLSSKGISLPLSCPMCDLDIEDMLHLFFKCDFAMECWN-----HVNL 261
            +WR C+ ++ V+ RL S+ IS+  +C +C    E + H  F C FA   W       + +
Sbjct: 1061 LWRACKGSLAVKGRLFSRHISVDATCSVCGDPDESINHALFDCTFARAIWQVSGFASLMM 1120

Query: 260  FYDMSNEVSATEWLLNKLSEASSDELVKICTVIWGLWFWRNKKVWDNKAVPASFAMDESF 81
               +S+     EWL      A+ +E   +C+ +W  WF RNK +++N+   A        
Sbjct: 1121 NAPLSSFSERLEWL---AKHATKEEFRTMCSFMWAGWFCRNKLIFENELSDAPLVAKRFS 1177

Query: 80   RSISSWRE 57
            + ++ + E
Sbjct: 1178 KLVADYCE 1185


>emb|CCA66050.1| hypothetical protein [Beta vulgaris subsp. vulgaris]
          Length = 1357

 Score =  330 bits (846), Expect(2) = e-108
 Identities = 172/407 (42%), Positives = 245/407 (60%)
 Frame = -1

Query: 1816 EGEVALKLDISKAYDRVSWRYLRHRLKVMGFCNTWASWMMMCVKSVSYNFYLNGSSIGPI 1637
            +G +A+KLD+SKAYDRV W +LR  L  MGF   W + +M CV +VSY+F +NG   G +
Sbjct: 575  KGLMAMKLDMSKAYDRVEWGFLRKLLLTMGFDGRWVNLVMSCVATVSYSFIINGRVCGSV 634

Query: 1636 VPKRGLRQGDPLSPYLFLLCVEGLSSAIDQANREGSLHGCCISPTAPTISHLLFADDSFL 1457
             P RGLRQGDPLSP+LF+L  +  S  + Q      +HG   S   P ISHLLFADDS L
Sbjct: 635  TPSRGLRQGDPLSPFLFILVADAFSQMVKQKVVSKEIHGAKASRNGPEISHLLFADDSLL 694

Query: 1456 FFRGTVEEANNIRALLFNYARCSGQSVNFQKSGVFFSANVRRDKQQQISSILGVFNDISS 1277
            F R T +E   I  +L  Y   SGQ +N++KS V FS  V  +K++++ ++L +      
Sbjct: 695  FTRATRQECLTIVDILNKYEAASGQKINYEKSEVSFSRGVSCEKKEELITLLHMRQVDRH 754

Query: 1276 SKYLGLPSLVGRSKKRVFGYLKERVSKRIQSWQSKSISRAGKTVLIRNVAQAIPAFTMSC 1097
             KYLG+P+L GRSKK +F  L +R+ K+++ W+ K +SRAGK VLI+ V QA+P + M  
Sbjct: 755  QKYLGIPALCGRSKKVLFRELLDRMWKKLRGWKEKLLSRAGKEVLIKAVIQALPTYLMGV 814

Query: 1096 FLLPKSLCQEIERKFNNYWWSSGKAGSKGLNWLSWKNMGLPKKKGGLGFRDLYGFNIALL 917
            + LP ++ QEI      +WW  GK   + ++WLSW+ M  PK  GG+GF+DL  FN ALL
Sbjct: 815  YKLPVAVIQEIHSAMARFWW-GGKGDERKMHWLSWEKMCKPKCMGGMGFKDLAVFNDALL 873

Query: 916  GKHCWNFMSNPLSLVARLFKARYYPSVHVLKATAGTGASFIWKGIWTAKEELKKGFRWVL 737
            GK  W  + N  SL++R+  A+YYP   V  A  G   S+ W+ IW AK  + +G  W +
Sbjct: 874  GKQVWRLLHNKESLLSRVMSAKYYPHGDVRYARLGYSHSYSWRSIWGAKSLVLEGLIWRV 933

Query: 736  GNGNDIVATKDLWLRNKKDYGVEQSHFYEGRSELVSSLINSNSKSWN 596
            G+G  I      W+ +++   ++ +   EG  E+V  L++   K WN
Sbjct: 934  GDGTKIDIWSAPWVGDEEGRFIKSAR-VEG-LEVVGDLMDVERKEWN 978



 Score = 92.0 bits (227), Expect(2) = e-108
 Identities = 50/199 (25%), Positives = 98/199 (49%), Gaps = 3/199 (1%)
 Frame = -3

Query: 590  VNDRVAWTGTSNGVYSAKSGYQFWFDRMVGTGDVPQN--QGWSKVWKLDIPHKVKIFVWR 417
            + D + W  + +G YS K+ Y      M+G G    +  + W+ +W L++  KV+ F+WR
Sbjct: 1004 LQDELTWAYSKDGTYSVKTAY------MLGKGGNLDDFHRVWNILWSLNVSPKVRHFLWR 1057

Query: 416  FCRNNVPVRMRLSSKGISLPLSCPMCDLDIEDMLHLFFKCDFAMECWNHVNLFYDMSN-E 240
             C +++PVR  L  + +     CP C  + E   HLF++C  +++ W  +  +  +   E
Sbjct: 1058 ACTSSLPVRKVLQRRHLIDEAGCPCCAREDETQFHLFYRCPMSLKLWEELGSYILLPGIE 1117

Query: 239  VSATEWLLNKLSEASSDELVKICTVIWGLWFWRNKKVWDNKAVPASFAMDESFRSISSWR 60
              A    L + S+  +  + K C ++W +W  RN++V+++ + PA+       R +  + 
Sbjct: 1118 DEAMCDTLVRWSQMDAKVVQKGCYILWNVWVERNRRVFEHTSQPATVVGQRIMRQVEDFN 1177

Query: 59   EARKKVKSSEPTGGVKMSS 3
                K+      GG++ S+
Sbjct: 1178 NYAVKI-----YGGMRSSA 1191


>pir||T00833 RNA-directed DNA polymerase homolog T13L16.7 - Arabidopsis thaliana
            (fragment)
          Length = 1365

 Score =  331 bits (848), Expect(2) = e-106
 Identities = 161/404 (39%), Positives = 241/404 (59%)
 Frame = -1

Query: 1807 VALKLDISKAYDRVSWRYLRHRLKVMGFCNTWASWMMMCVKSVSYNFYLNGSSIGPIVPK 1628
            +A K D+SKAYDRV W +L   +  +GF N W SW+M CV SVSY+  +NG   G I+P 
Sbjct: 570  MAFKTDMSKAYDRVEWPFLETMMTALGFNNKWISWIMNCVTSVSYSVLINGQPYGHIIPT 629

Query: 1627 RGLRQGDPLSPYLFLLCVEGLSSAIDQANREGSLHGCCISPTAPTISHLLFADDSFLFFR 1448
            RG+RQGDPLSP LF+LC E L   +++A + G + G        +++HLLFADD+ L  +
Sbjct: 630  RGIRQGDPLSPALFVLCTEALIHILNKAEQAGKITGIQFQDKKVSVNHLLFADDTLLMCK 689

Query: 1447 GTVEEANNIRALLFNYARCSGQSVNFQKSGVFFSANVRRDKQQQISSILGVFNDISSSKY 1268
             T +E   +   L  Y + SGQ +N  KS + F  NV    +  I S  G+  +  + KY
Sbjct: 690  ATKQECEELMQCLSQYGQLSGQMINLNKSAITFGKNVDIQIKDWIKSRSGISLEGGTGKY 749

Query: 1267 LGLPSLVGRSKKRVFGYLKERVSKRIQSWQSKSISRAGKTVLIRNVAQAIPAFTMSCFLL 1088
            LGLP  +  SK+ +FG++KE++  R+  W +K++S+ GK VL++++A A+P + MSCF L
Sbjct: 750  LGLPECLSGSKRDLFGFIKEKLQSRLTGWYAKTLSQGGKEVLLKSIALALPVYVMSCFKL 809

Query: 1087 PKSLCQEIERKFNNYWWSSGKAGSKGLNWLSWKNMGLPKKKGGLGFRDLYGFNIALLGKH 908
            PK+LCQ++     ++WW+S +   K ++WLSW+ + LPK +GG GF+DL  FN ALL K 
Sbjct: 810  PKNLCQKLTTVMMDFWWNSMQQKRK-IHWLSWQRLTLPKDQGGFGFKDLQCFNQALLAKQ 868

Query: 907  CWNFMSNPLSLVARLFKARYYPSVHVLKATAGTGASFIWKGIWTAKEELKKGFRWVLGNG 728
             W  +    SL +R+F++RY+ +   L AT G+  S+ W+ I   +E L +G R V+GNG
Sbjct: 869  AWRVLQEKGSLFSRVFQSRYFSNSDFLSATRGSRPSYAWRSILFGRELLMQGLRTVIGNG 928

Query: 727  NDIVATKDLWLRNKKDYGVEQSHFYEGRSELVSSLINSNSKSWN 596
                   D WL +  +        +      VS LI+  S++WN
Sbjct: 929  QKTFVWTDKWLHDGSNRRPLNRRRFINVDLKVSQLIDPTSRNWN 972



 Score = 84.0 bits (206), Expect(2) = e-106
 Identities = 52/197 (26%), Positives = 94/197 (47%), Gaps = 12/197 (6%)
 Frame = -3

Query: 584  DRVAWTGTSNGVYSAKSGYQFW----FDRMVGTGDVPQ--NQGWSKVWKLDIPHKVKIFV 423
            D   W  + NG+YS K+GY+F       R+     V    N  + K+W L    K++IF+
Sbjct: 999  DSFCWLHSHNGLYSVKTGYEFLSKQVHHRLYQEAKVKPSVNSLFDKIWNLHTAPKIRIFL 1058

Query: 422  WRFCRNNVPVRMRLSSKGISLPLSCPMCDLDIEDMLHLFFKCDFAMECWNHVNLF---YD 252
            W+     +PV  RL ++GI     C MCD + E + H+ F+C  A + W   +L     +
Sbjct: 1059 WKALHGAIPVEDRLRTRGIRSDDGCLMCDTENETINHILFECPLARQVWAITHLSSAGSE 1118

Query: 251  MSNEVSATEWLLNKLSEASS-DELVKICT--VIWGLWFWRNKKVWDNKAVPASFAMDESF 81
             SN V      L  L++ +     ++  +  ++W LW  RN  +++ K    +  +D+++
Sbjct: 1119 FSNSVYTNMSRLIDLTQQNDLPHHLRFVSPWILWFLWKNRNALLFEGKGSITTTLVDKAY 1178

Query: 80   RSISSWREARKKVKSSE 30
             +   W  A+  +++ E
Sbjct: 1179 EAYHEWFSAQTHMQNDE 1195