BLASTX nr result
ID: Bupleurum21_contig00027147
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00027147
(537 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003598202.1| 60S ribosomal protein L23 [Medicago truncatu... 61 1e-14
ref|XP_003615225.1| hypothetical protein MTR_5g065390 [Medicago ... 50 6e-09
ref|XP_003617799.1| hypothetical protein MTR_5g095600 [Medicago ... 41 5e-07
ref|XP_003613452.1| hypothetical protein MTR_5g036840 [Medicago ... 46 9e-07
gb|ABD32189.2| Polynucleotidyl transferase, Ribonuclease H fold ... 42 2e-06
>ref|XP_003598202.1| 60S ribosomal protein L23 [Medicago truncatula]
gi|355487250|gb|AES68453.1| 60S ribosomal protein L23
[Medicago truncatula]
Length = 547
Score = 60.8 bits (146), Expect(2) = 1e-14
Identities = 33/110 (30%), Positives = 57/110 (51%)
Frame = +1
Query: 208 AQQNRGNFSTSLVLRNHMGAFVAGKVTCMSGVSTAFEAEASAIFEGLNWLLTMQLNRVVI 387
++QNR F T + +R+ G F+ G EAEA + + + WL ++L+ V +
Sbjct: 75 SEQNR--FGTGMCVRDQRGRFLKAATNWYEGCPPPQEAEAVGLRDAILWLGQLELSNVQL 132
Query: 388 KSDSLLSIQVICRSSDNLLEVGHVLDSCHTILYSRPEFSLSFVKR*ANEI 537
+ D L + I ++N E G ++D C ++L F +SFV+R AN +
Sbjct: 133 ELDCKLVVDSIYDKNNNQAEFGSIIDDCRSLLQQFTNFKISFVRRQANVV 182
Score = 43.5 bits (101), Expect(2) = 1e-14
Identities = 25/66 (37%), Positives = 34/66 (51%)
Frame = +2
Query: 11 NKKVWENKLVTPNIAMNRSAKFFSDWKLAKLSRAVMQIPSKSIVPSVTQRWQPPEEDCLK 190
N KVW+ +L NIA+ + + W+ A+ S A Q+ P T WQPP LK
Sbjct: 11 NDKVWDGQLQPSNIAVQLARELLFQWQAARNS-ATTQVQQS---PQDTIAWQPPAAGFLK 66
Query: 191 LNVDAS 208
NVDA+
Sbjct: 67 CNVDAA 72
>ref|XP_003615225.1| hypothetical protein MTR_5g065390 [Medicago truncatula]
gi|355516560|gb|AES98183.1| hypothetical protein
MTR_5g065390 [Medicago truncatula]
Length = 365
Score = 50.4 bits (119), Expect(2) = 6e-09
Identities = 25/93 (26%), Positives = 46/93 (49%)
Frame = +1
Query: 232 STSLVLRNHMGAFVAGKVTCMSGVSTAFEAEASAIFEGLNWLLTMQLNRVVIKSDSLLSI 411
S + LR+ G FV K+ +GV +A E +F + WL M++N + + DS ++
Sbjct: 269 SVGICLRDDEGIFVLAKIVSFAGVYSAEVGETLGLFHTIQWLSDMKMNNIDFEVDSKITK 328
Query: 412 QVICRSSDNLLEVGHVLDSCHTILYSRPEFSLS 510
+++ E GH++ ++ +S F LS
Sbjct: 329 DAFTSRREDIFEFGHIVVCSFSLYFSFQIFQLS 361
Score = 34.7 bits (78), Expect(2) = 6e-09
Identities = 23/85 (27%), Positives = 35/85 (41%), Gaps = 19/85 (22%)
Frame = +2
Query: 11 NKKVWENKLVTPNIAMNRSAKFFSDWKLAKLSRAVMQ------------------IPSKS 136
N KVWE+ T + + R+ DW+LA R MQ +P+
Sbjct: 176 NLKVWEDVTETWAVVIERARVLLDDWELANGLRTSMQPAPLGLQQQSDAGASSVVVPAPF 235
Query: 137 IVPSVTQ-RWQPPEEDCLKLNVDAS 208
+ ++ Q WQ P LK N+D +
Sbjct: 236 VTIALHQINWQSPASSRLKCNIDTT 260
>ref|XP_003617799.1| hypothetical protein MTR_5g095600 [Medicago truncatula]
gi|355519134|gb|AET00758.1| hypothetical protein
MTR_5g095600 [Medicago truncatula]
Length = 192
Score = 41.2 bits (95), Expect(2) = 5e-07
Identities = 23/71 (32%), Positives = 39/71 (54%)
Frame = +1
Query: 325 ASAIFEGLNWLLTMQLNRVVIKSDSLLSIQVICRSSDNLLEVGHVLDSCHTILYSRPEFS 504
A + E + WL + L++V I+ D L + I S + E G++L C ++L P F
Sbjct: 120 AIGLKEAIIWLGELGLSKVSIELDCKLVVDDIGDRSVSQSEFGNLLSLCRSLLIQYPNFE 179
Query: 505 LSFVKR*ANEI 537
++FV+R AN +
Sbjct: 180 INFVRRQANYV 190
Score = 37.4 bits (85), Expect(2) = 5e-07
Identities = 20/69 (28%), Positives = 38/69 (55%), Gaps = 3/69 (4%)
Frame = +2
Query: 11 NKKVWENKLVTPNIAMNRSAKFFSDW---KLAKLSRAVMQIPSKSIVPSVTQRWQPPEED 181
N+KVW +++ P+I++ + +W ++ K + AV QI + + W+PP +
Sbjct: 50 NEKVWHDEVKEPHISIQLARDTLREWLSVRVNKATPAVQQIVTDT-------NWKPPAKG 102
Query: 182 CLKLNVDAS 208
+K NVDA+
Sbjct: 103 YVKCNVDAA 111
>ref|XP_003613452.1| hypothetical protein MTR_5g036840 [Medicago truncatula]
gi|355514787|gb|AES96410.1| hypothetical protein
MTR_5g036840 [Medicago truncatula]
Length = 261
Score = 46.2 bits (108), Expect(2) = 9e-07
Identities = 23/83 (27%), Positives = 42/83 (50%)
Frame = +1
Query: 232 STSLVLRNHMGAFVAGKVTCMSGVSTAFEAEASAIFEGLNWLLTMQLNRVVIKSDSLLSI 411
S + +R+ G+FV K + EA + L WL MQL+ V ++DS +++
Sbjct: 94 SIGICIRDSEGSFVLSKTITSLCIVLVDVGEALGLHSALQWLSDMQLDNVDFETDSKVTV 153
Query: 412 QVICRSSDNLLEVGHVLDSCHTI 480
V + ++L E G ++ SC ++
Sbjct: 154 DVFLSTRNDLSEFGSIISSCRSL 176
Score = 31.6 bits (70), Expect(2) = 9e-07
Identities = 18/77 (23%), Positives = 30/77 (38%), Gaps = 11/77 (14%)
Frame = +2
Query: 11 NKKVWENKLVTPNIAMNRSAKFFSDWKLAKLSRAVMQIPSKSIVPSVTQR---------- 160
N +VW++ + + R+ + W+LA + V + R
Sbjct: 9 NLRVWDDVTERSPVVVERARNMITYWQLANAPDVLASTSPPQHVMAAHMRASSSHQHTRI 68
Query: 161 -WQPPEEDCLKLNVDAS 208
WQPP D K N+DA+
Sbjct: 69 LWQPPTSDRYKCNIDAA 85
>gb|ABD32189.2| Polynucleotidyl transferase, Ribonuclease H fold [Medicago
truncatula]
Length = 612
Score = 42.0 bits (97), Expect(2) = 2e-06
Identities = 23/106 (21%), Positives = 46/106 (43%), Gaps = 1/106 (0%)
Frame = +1
Query: 217 NRGNFSTSLVLRNHMGAFVAGKVTCMSGVSTAFEAEASAIFEGLNWLLTMQLNRVVIKSD 396
++ + +R+ GA+V K V EA ++ L WL MQ + + D
Sbjct: 472 SKNRIGIGICVRDDDGAYVLAKTISFDVVHPVRVGEALGLYHALEWLSDMQFDNMDFAMD 531
Query: 397 SLLSIQVICRSSDNLLEVGHVLDSCHTILYSR-PEFSLSFVKR*AN 531
S ++ D++ E GH++ + ++ + + F++R AN
Sbjct: 532 SKITYDAFNSQKDDISEFGHIISARQSLFSTHFTNSRVEFIRRQAN 577
Score = 34.7 bits (78), Expect(2) = 2e-06
Identities = 26/95 (27%), Positives = 41/95 (43%), Gaps = 30/95 (31%)
Frame = +2
Query: 11 NKKVWENKLVTPNIAMNRSAKFFSDWKLAKLSRAV-------MQIPS------------- 130
N KVWENK + + ++R+ +W+LA +R+ +Q PS
Sbjct: 375 NLKVWENKNESCAMTVDRARVMMEEWQLATATRSAPTHQHTHLQNPSAATAQQPVVHLQQ 434
Query: 131 ----------KSIVPSVTQRWQPPEEDCLKLNVDA 205
+S+VPS++ W+ P K NVDA
Sbjct: 435 SHVASAVSAQQSLVPSIS--WERPSRGRFKCNVDA 467