BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00026991
         (1067 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002510498.1| cytochrome P450, putative [Ricinus communis]...   296   4e-78
emb|CBI19823.3| unnamed protein product [Vitis vinifera]              284   2e-74
ref|XP_002280472.1| PREDICTED: cytochrome P450 83B1 [Vitis vinif...   282   9e-74
ref|XP_002278300.1| PREDICTED: cytochrome P450 83B1 [Vitis vinif...   281   3e-73
ref|XP_002510500.1| cytochrome P450, putative [Ricinus communis]...   280   3e-73

>ref|XP_002510498.1| cytochrome P450, putative [Ricinus communis]
            gi|223551199|gb|EEF52685.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 496

 Score =  296 bits (759), Expect = 4e-78
 Identities = 159/318 (50%), Positives = 215/318 (67%), Gaps = 3/318 (0%)
 Frame = +1

Query: 121  MEVVLILLAISLPLYMIVKYCRNYYGHRSNXXXXXXXXXXXXIGNMHQFDSTNTHIYLFQ 300
            M ++  LL   + L  ++K       HR+             IGN+ Q D++N   +L+Q
Sbjct: 1    MALLFFLLLFPILLLFLLKK------HRATPHLPPGPKGFPFIGNLLQLDNSNIQKHLWQ 54

Query: 301  LSKKYGPLMSLQLGSVRTLVVSSASAAKQVFKYHDLCFSSRPVLVGTQKVSYNGIDVVFA 480
            LSKKYGP+MSL+LG   TL+VSSA  A++V K  DL F SRP L G QK+SYNG+D+ FA
Sbjct: 55   LSKKYGPIMSLRLGFKPTLIVSSAKMAREVLKTQDLEFCSRPALTGQQKLSYNGLDLAFA 114

Query: 481  PYDEYWRNMRKICTLHLFSAKRSQSFRPIREEEVARMVKKIRDGAVDDSNIVNLSKTLMT 660
            PYD+YWR MRKI  +HLF++ R QSFRPIRE+EV+RM++ +   A  DS  VNL++ +M 
Sbjct: 115  PYDDYWREMRKIGVVHLFNSNRVQSFRPIREDEVSRMIRNVSKLA-SDSKPVNLTEEMMA 173

Query: 661  LTSSIIFRITFGKRYDNGDEKDDMSGRFHWLLHETQANFVSFFFADYFPVIGPLIDRLSG 840
            LTS+ I R+ FGKRY +G  +   + R H LL+ETQA F +FFF+DYFP +G ++D+LSG
Sbjct: 174  LTSAAICRVAFGKRYKDGGNE---AKRLHQLLNETQALFAAFFFSDYFPYVGWIVDKLSG 230

Query: 841  ACARLEKSFNELDAFYQQLIDEHLHASSTVSAQDCSILDILLQMRTDSS---DFTFDHVK 1011
              +RLE +F+E D FYQ+LIDEHL     +   D + LD+LLQ++ D S     TFDH+K
Sbjct: 231  LLSRLETNFHEFDIFYQELIDEHLDPEREMPEHD-NFLDVLLQIQKDRSIKIQLTFDHIK 289

Query: 1012 ALLMNVIVAASDTSAAAV 1065
            A+LMN+ VA +DTSAAAV
Sbjct: 290  AILMNIFVAGTDTSAAAV 307


>emb|CBI19823.3| unnamed protein product [Vitis vinifera]
          Length = 912

 Score =  284 bits (727), Expect = 2e-74
 Identities = 162/362 (44%), Positives = 233/362 (64%), Gaps = 7/362 (1%)
 Frame = +1

Query: 1    AISCFLLIPSSLSAFSIFFIHTCHHNSTYILLLHRVGIKKMEVVLILLAISLPLYMIVKY 180
            +I  + + P +L+  +I     C+     I L H         VL+ + ++ PL ++  +
Sbjct: 379  SIDGYEIPPKTLTMKAIIVTSPCN-----IALTHSSEDLHTMTVLLFVILAFPLLLLFLH 433

Query: 181  CRNYYGHRSNXXXXXXXXXXXX---IGNMHQFDSTNTHIYLFQLSKKYGPLMSLQLGSVR 351
             +    HR N               IGN+HQ D++  H YL+QLSK+YG LMSL+LG + 
Sbjct: 434  RK----HRKNGGLLHLPPGPPGLPFIGNLHQMDNSARHRYLWQLSKQYGSLMSLRLGFIP 489

Query: 352  TLVVSSASAAKQVFKYHDLCFSSRPVLVGTQKVSYNGIDVVFAPYDEYWRNMRKICTLHL 531
            T+VVSSA  AK+V K HDL F+SRP L+G Q++SYN +D+ F+PY++YWR MRKIC LHL
Sbjct: 490  TIVVSSARIAKEVMKTHDLEFASRPSLIGPQRLSYNCLDLAFSPYNDYWREMRKICVLHL 549

Query: 532  FSAKRSQSFRPIREEEVARMVKKIRDGAVDDSNIVNLSKTLMTLTSSIIFRITFGKRY-D 708
            F+ KR QS+ PIRE EV++M++KI   A   S ++NLS+TLM LTS+II R+ FGKRY D
Sbjct: 550  FTLKRVQSYTPIREYEVSQMIEKISKLA-SASKLINLSETLMFLTSTIICRVAFGKRYED 608

Query: 709  NGDEKDDMSGRFHWLLHETQANFVSFFFADYFPVIGPLIDRLSGACARLEKSFNELDAFY 888
             G E+     RFH LL++ QA   SFFF+D+FP+IG  +D+L+G  ARLEK+F ++D FY
Sbjct: 609  EGFER----SRFHGLLNDAQAMLGSFFFSDHFPLIG-WLDKLTGLTARLEKTFRDMDLFY 663

Query: 889  QQLIDEHLHASSTVSAQDCSILDILLQMRTDSS---DFTFDHVKALLMNVIVAASDTSAA 1059
            Q++I++HL        Q+  I D+L+ ++ D+S   D T+DH+K +LMN+ V  +DT AA
Sbjct: 664  QEIIEDHLKPDRKKQEQE-DITDVLIGLQKDNSFAIDITWDHIKGVLMNIFVGGTDTGAA 722

Query: 1060 AV 1065
             V
Sbjct: 723  TV 724



 Score =  275 bits (704), Expect = 1e-71
 Identities = 148/319 (46%), Positives = 217/319 (68%), Gaps = 7/319 (2%)
 Frame = +1

Query: 130  VLILLAISLPLYMIVKYCRNYYGHRSNXXXXXXXXXXXX---IGNMHQFDSTNTHIYLFQ 300
            VL+ + ++ PL ++  + +    HR N               IGN+HQ D++  H YL+Q
Sbjct: 3    VLLFVILAFPLLLLFLHRK----HRKNGGLLHLPPGPPGLPVIGNLHQMDNSAPHRYLWQ 58

Query: 301  LSKKYGPLMSLQLGSVRTLVVSSASAAKQVFKYHDLCFSSRPVLVGTQKVSYNGIDVVFA 480
            LSK+YGPLMSL+LG + T+VVSSA  AK+V K HDL F+SRP L+G +++SYN +D+ F+
Sbjct: 59   LSKQYGPLMSLRLGFIPTIVVSSARIAKEVMKTHDLKFASRPSLIGPRRLSYNCLDLAFS 118

Query: 481  PYDEYWRNMRKICTLHLFSAKRSQSFRPIREEEVARMVKKIRDGAVDDSNIVNLSKTLMT 660
            PY++YWR MRKIC LHLF+ KR QS+ PIRE EV++M++KI   A   S ++NLS+T+M 
Sbjct: 119  PYNDYWREMRKICVLHLFTLKRVQSYTPIREYEVSQMIEKISKLA-SASKLINLSETVMF 177

Query: 661  LTSSIIFRITFGKRY-DNGDEKDDMSGRFHWLLHETQANFVSFFFADYFPVIGPLIDRLS 837
            LT +II R++FGKRY D G E    + RFH LL++ QA   SFFF+D+FP++G  +D+L+
Sbjct: 178  LTITIICRVSFGKRYEDEGCE----TSRFHGLLNDAQAMLGSFFFSDHFPLMG-WLDKLT 232

Query: 838  GACARLEKSFNELDAFYQQLIDEHLHASSTVSAQDCSILDILLQMRTDSS---DFTFDHV 1008
            G  ARLEK+  ++D FYQ++I++HL        Q+  I D+L++++ D+S   D T+DH+
Sbjct: 233  GLTARLEKTLRDMDLFYQEIIEDHLKPDRKKQEQE-DITDVLIELQKDNSFAIDITWDHI 291

Query: 1009 KALLMNVIVAASDTSAAAV 1065
            K +LMN+ V  +D   A V
Sbjct: 292  KGVLMNIFVGGTDAGTATV 310


>ref|XP_002280472.1| PREDICTED: cytochrome P450 83B1 [Vitis vinifera]
            gi|147832399|emb|CAN64422.1| hypothetical protein
            VITISV_032274 [Vitis vinifera]
          Length = 498

 Score =  282 bits (722), Expect = 9e-74
 Identities = 154/319 (48%), Positives = 218/319 (68%), Gaps = 7/319 (2%)
 Frame = +1

Query: 130  VLILLAISLPLYMIVKYCRNYYGHRSNXXXXXXXXXXXX---IGNMHQFDSTNTHIYLFQ 300
            VL+ + ++ PL ++  + +    HR N               IGN+HQ D++  H YL+Q
Sbjct: 3    VLLFVILAFPLLLLFLHRK----HRKNGGLLHLPPGPPGLPFIGNLHQMDNSARHRYLWQ 58

Query: 301  LSKKYGPLMSLQLGSVRTLVVSSASAAKQVFKYHDLCFSSRPVLVGTQKVSYNGIDVVFA 480
            LSK+YG LMSL+LG + T+VVSSA  AK+V K HDL F+SRP L+G Q++SYN +D+ F+
Sbjct: 59   LSKQYGSLMSLRLGFIPTIVVSSARIAKEVMKTHDLEFASRPSLIGPQRLSYNCLDLAFS 118

Query: 481  PYDEYWRNMRKICTLHLFSAKRSQSFRPIREEEVARMVKKIRDGAVDDSNIVNLSKTLMT 660
            PY++YWR MRKIC LHLF+ KR QS+ PIRE EV++M++KI   A   S ++NLS+TLM 
Sbjct: 119  PYNDYWREMRKICVLHLFTLKRVQSYTPIREYEVSQMIEKISKLA-SASKLINLSETLMF 177

Query: 661  LTSSIIFRITFGKRY-DNGDEKDDMSGRFHWLLHETQANFVSFFFADYFPVIGPLIDRLS 837
            LTS+II R+ FGKRY D G E+     RFH LL++ QA   SFFF+D+FP+IG  +D+L+
Sbjct: 178  LTSTIICRVAFGKRYEDEGFER----SRFHGLLNDAQAMLGSFFFSDHFPLIG-WLDKLT 232

Query: 838  GACARLEKSFNELDAFYQQLIDEHLHASSTVSAQDCSILDILLQMRTDSS---DFTFDHV 1008
            G  ARLEK+F ++D FYQ++I++HL        Q+  I D+L+ ++ D+S   D T+DH+
Sbjct: 233  GLTARLEKTFRDMDLFYQEIIEDHLKPDRKKQEQE-DITDVLIGLQKDNSFAIDITWDHI 291

Query: 1009 KALLMNVIVAASDTSAAAV 1065
            K +LMN+ V  +DT AA V
Sbjct: 292  KGVLMNIFVGGTDTGAATV 310


>ref|XP_002278300.1| PREDICTED: cytochrome P450 83B1 [Vitis vinifera]
          Length = 495

 Score =  281 bits (718), Expect = 3e-73
 Identities = 157/316 (49%), Positives = 217/316 (68%), Gaps = 3/316 (0%)
 Frame = +1

Query: 127  VVLILLAISLPLYMIVKYCRNYYGHRSNXXXXXXXXXXXXIGNMHQFDSTNTHIYLFQLS 306
            ++L+L+ ++LPL+++    RN    R              IGN+ Q D +  HIYL++LS
Sbjct: 2    MILLLILLALPLFLLF-LLRN----RRRTPLPPGPPGLPLIGNLLQLDKSAPHIYLWRLS 56

Query: 307  KKYGPLMSLQLGSVRTLVVSSASAAKQVFKYHDLCFSSRPVLVGTQKVSYNGIDVVFAPY 486
            K+YGPLM L+LG V TLVVSSA  AK+V K HDL FS RP L+G +K+SYNG+DV F+PY
Sbjct: 57   KQYGPLMILRLGFVPTLVVSSARMAKEVMKTHDLEFSGRPSLLGLRKLSYNGLDVAFSPY 116

Query: 487  DEYWRNMRKICTLHLFSAKRSQSFRPIREEEVARMVKKIRDGAVDDSNIVNLSKTLMTLT 666
            ++YWR MRKIC LHLF++KR+QSFRPIRE+EV  M+KKI   A   S + NLS+ L++LT
Sbjct: 117  NDYWREMRKICVLHLFNSKRAQSFRPIREDEVLEMIKKISQFA-SASKLTNLSEILISLT 175

Query: 667  SSIIFRITFGKRYDNGDEKDDMSGRFHWLLHETQANFVSFFFADYFPVIGPLIDRLSGAC 846
            S+II R+ F KRYD  DE  + S RF  L+ E QA    F+F+DYFP++G  +D+L+G  
Sbjct: 176  STIICRVAFSKRYD--DEGYERS-RFQKLVGEGQAVVGGFYFSDYFPLMG-WVDKLTGMI 231

Query: 847  ARLEKSFNELDAFYQQLIDEHLHASSTVSAQDCSILDILLQM---RTDSSDFTFDHVKAL 1017
            A  +K+F E D FYQ++IDEHL  +     ++  I D+LL++   R  + D TFDH+KA+
Sbjct: 232  ALADKNFKEFDLFYQEIIDEHLDPNRPEPEKE-DITDVLLKLQKNRLFTIDLTFDHIKAV 290

Query: 1018 LMNVIVAASDTSAAAV 1065
            LMN+ +A +DTSAA +
Sbjct: 291  LMNIFLAGTDTSAATL 306


>ref|XP_002510500.1| cytochrome P450, putative [Ricinus communis]
            gi|223551201|gb|EEF52687.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 497

 Score =  280 bits (717), Expect = 3e-73
 Identities = 159/313 (50%), Positives = 207/313 (66%), Gaps = 3/313 (0%)
 Frame = +1

Query: 136  ILLAISLPLYMIVKYCRNYYGHRSNXXXXXXXXXXXXIGNMHQFDSTNTHIYLFQLSKKY 315
            I L ++LP+++   +       R N            IGN+HQFD +    YL++LS+KY
Sbjct: 5    IFLVLALPVFL--SFLLQKLKTRRNSSLPPGPKGLPLIGNLHQFDQSAPQNYLWKLSQKY 62

Query: 316  GPLMSLQLGSVRTLVVSSASAAKQVFKYHDLCFSSRPVLVGTQKVSYNGIDVVFAPYDEY 495
            GPLMSL+LGSV  LVVSSA  AK + K +DL F SRP ++G QK+SYNG+D+ FAPY+ Y
Sbjct: 63   GPLMSLRLGSVPILVVSSAKMAKDILKTYDLTFCSRPPVLGQQKLSYNGLDLAFAPYNSY 122

Query: 496  WRNMRKICTLHLFSAKRSQSFRPIREEEVARMVKKIRDGAVDDSNIVNLSKTLMTLTSSI 675
            WR MRKIC +HLF++ + QSFRPIRE EV+ M++KI   A   +  V+LS+ +M+LTS+I
Sbjct: 123  WREMRKICVVHLFNSNKVQSFRPIREFEVSHMLEKISKLAA-ATKPVDLSEAMMSLTSTI 181

Query: 676  IFRITFGKRYDNGDEKDDMSGRFHWLLHETQANFVSFFFADYFPVIGPLIDRLSGACARL 855
            I R+ FGKRY   +E+     RF  LL ETQA F SFF +DYFP +G  +DRL+G   RL
Sbjct: 182  ICRVAFGKRY---EEEGIERTRFQALLEETQALFTSFFVSDYFPFLG-FVDRLTGMNRRL 237

Query: 856  EKSFNELDAFYQQLIDEHLHASSTVSAQDCSILDILLQMRTDSS---DFTFDHVKALLMN 1026
            EK+F E D FY Q+I EHL  S     Q+  ILD+LLQ+  D S     T DH+KA+LMN
Sbjct: 238  EKNFKEFDIFYNQIIQEHLDPSRPKPEQE-DILDVLLQIWKDRSFKAHLTPDHIKAILMN 296

Query: 1027 VIVAASDTSAAAV 1065
            V V  +DTSAAAV
Sbjct: 297  VFVGGTDTSAAAV 309