BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00026732
         (1080 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002532719.1| ubiquitin-protein ligase, putative [Ricinus ...   152   9e-48
ref|XP_002297705.1| predicted protein [Populus trichocarpa] gi|2...   130   1e-41
ref|XP_003534732.1| PREDICTED: F-box protein CPR30-like [Glycine...   143   5e-39
ref|XP_003610528.1| F-box family protein [Medicago truncatula] g...   132   3e-37
ref|XP_003546182.1| PREDICTED: F-box protein CPR30-like [Glycine...   133   4e-37

>ref|XP_002532719.1| ubiquitin-protein ligase, putative [Ricinus communis]
           gi|223527546|gb|EEF29668.1| ubiquitin-protein ligase,
           putative [Ricinus communis]
          Length = 389

 Score =  152 bits (385), Expect(2) = 9e-48
 Identities = 93/216 (43%), Positives = 126/216 (58%), Gaps = 18/216 (8%)
 Frame = +1

Query: 154 MSDVPPELITDIFTRLPVKSLLRFRSVSKPYRALIDSPKFIKSHLNQSLKSKSHQSIVVS 333
           MS++P E+I +I  RL  K LL  R VSK +R LIDSP FI  HLN S++S  + SI++ 
Sbjct: 1   MSNLPLEMIAEILCRLTAKKLLCCRCVSKRWRTLIDSPTFIYLHLNHSIESPCNLSIILK 60

Query: 334 LNDDTDQLYSIDF------ESLDKPTLLTQLGTCVIGSFNGLLCI-KYGHRITLFNPETR 492
               + +LYS+ F      + LD P +    G  ++GS NGLLCI      I L+NP  R
Sbjct: 61  ----SSELYSLSFDLLDNIQPLDHPLMCYNHGVKILGSCNGLLCICNIVDDIALWNPSIR 116

Query: 493 MHRTVP-------MFYTIRPCDFSVYGFGYDHVNDDYKVVWI---GRVENGS-TGEFMVY 639
            HR VP        ++ +  C  SV+GFGYD  NDDYK+V I   G V+  S   E  V+
Sbjct: 117 AHRVVPYLPVELKRYFGMCSCRVSVFGFGYDLSNDDYKLVRIAQFGGVDRKSFESEVKVF 176

Query: 640 SLKSDSWRKIADFPYYLCSKRVHNVFVNGALHWLVT 747
           SL+ +SWR+IAD PY +     + ++ NGALHWLV+
Sbjct: 177 SLRKNSWRRIADMPYCVLYPGENGIYANGALHWLVS 212



 Score = 65.1 bits (157), Expect(2) = 9e-48
 Identities = 32/69 (46%), Positives = 47/69 (68%)
 Frame = +2

Query: 869  AFDLATEEYRMVPQPEFFGMSVLMSLGVLEGKLCINTNFYMKGMDCMGIWVMEQYGVKES 1048
            A DL  E+Y +VP+PEF  M+  M +GVL+G  C++   Y +  + + +WVME+Y VKES
Sbjct: 225  ALDLGVEDYHVVPKPEFVDMNCNMGVGVLQG--CLSLLAYARS-ERVDVWVMEEYMVKES 281

Query: 1049 WTKLLSVEK 1075
            W+KL SV +
Sbjct: 282  WSKLFSVAR 290


>ref|XP_002297705.1| predicted protein [Populus trichocarpa] gi|222844963|gb|EEE82510.1|
           predicted protein [Populus trichocarpa]
          Length = 408

 Score =  130 bits (328), Expect(2) = 1e-41
 Identities = 88/224 (39%), Positives = 120/224 (53%), Gaps = 30/224 (13%)
 Frame = +1

Query: 169 PELITDIFTRLPVKSLLRFRSVSKPYRALIDSPKFIKSHLNQSLKSKSHQSIVV--SLND 342
           PE+  DI +RLPVKSL RFR VSK +   IDSP FI +HL +S ++ +H ++++  + N 
Sbjct: 5   PEITADILSRLPVKSLKRFRCVSKSWCKEIDSPYFINTHLKRSSQAHTHLNLILRDATNL 64

Query: 343 DTDQLYSIDFESLD---KPTLLTQLGTCVIGSFNGLLC-IKYGHRITLFNPETRMHRTVP 510
            T  L S DF S++    P       T V+GS NGLL  +     I L+NP TR  + +P
Sbjct: 65  CTVDLDSPDFTSIELKNNPLKSDDCATEVMGSCNGLLALLNSDFSIALYNPSTREKKMIP 124

Query: 511 MFYTIRPCD---------FSVYGFGYDHVNDDYKVVWIGRVENGSTGEFM-----VYSLK 648
           +     P D         F+ YGFG+D +N+DYKVV        S   F      VYSLK
Sbjct: 125 VSPLELPNDLDDSKVSSLFNFYGFGHDPINEDYKVVRFIHFYGDSPDGFFHCEVKVYSLK 184

Query: 649 SDSWRKIADFPYYL----------CSKRVHNVFVNGALHWLVTV 750
           S+SW++I D+PY L            +R + VF N A+HW  TV
Sbjct: 185 SNSWKRIDDYPYDLRFILPPDYHPRCRRGYGVFANSAVHWKATV 228



 Score = 66.2 bits (160), Expect(2) = 1e-41
 Identities = 32/67 (47%), Positives = 46/67 (68%)
 Frame = +2

Query: 869  AFDLATEEYRMVPQPEFFGMSVLMSLGVLEGKLCINTNFYMKGMDCMGIWVMEQYGVKES 1048
            AFDL  EE++++PQP++      M++GVL G LC+  N   K ++   IWVM++YGVKES
Sbjct: 242  AFDLGAEEFKIIPQPDYSSNEHEMNVGVLGGCLCVFCNKNCKQVE---IWVMKEYGVKES 298

Query: 1049 WTKLLSV 1069
            WT L +V
Sbjct: 299  WTHLCTV 305


>ref|XP_003534732.1| PREDICTED: F-box protein CPR30-like [Glycine max]
          Length = 392

 Score =  143 bits (361), Expect(2) = 5e-39
 Identities = 85/220 (38%), Positives = 123/220 (55%), Gaps = 20/220 (9%)
 Frame = +1

Query: 154 MSD-VPPELITDIFTRLPVKSLLRFRSVSKPYRALIDSPKFIKSHLNQSLKSKSHQSIVV 330
           MSD +P E++TDI +RLP KSLLRFRS SK +++LIDS  F   HL++SL   S+ ++++
Sbjct: 1   MSDHLPREVVTDILSRLPAKSLLRFRSTSKSWKSLIDSQHFNSVHLSRSLSLTSNTTLIL 60

Query: 331 SLNDDTDQLYSIDFESLDKPTLLTQLGTC------VIGSFNGLLCI-KYGHRITLFNPET 489
            L+ D   LY  +F +LD P  L     C      ++GS NGLLCI      I  +NP  
Sbjct: 61  RLDSD---LYQTNFPTLDPPLFLNHPLMCYSNNITLLGSCNGLLCISNVADDIAFWNPSL 117

Query: 490 RMHRTVPMFYTIRP--------CDFSVYGFGYDHVNDDYKVV----WIGRVENGSTGEFM 633
           R HR +P     R             VYGFG+DH + DYK+V    ++   +     +  
Sbjct: 118 RQHRILPSLPLPRRRLHPDTTLFAARVYGFGFDHTSPDYKLVRISYFVDLQDRSFDSQVK 177

Query: 634 VYSLKSDSWRKIADFPYYLCSKRVHNVFVNGALHWLVTVK 753
           +Y+L++++W+ +   PY LC  R   VFV  +LHW+VT K
Sbjct: 178 LYTLRANAWKTLPSMPYALCCARTMGVFVGNSLHWVVTRK 217



 Score = 45.1 bits (105), Expect(2) = 5e-39
 Identities = 24/71 (33%), Positives = 39/71 (54%), Gaps = 2/71 (2%)
 Frame = +2

Query: 869  AFDLATEEYRMVPQPEFFGMS--VLMSLGVLEGKLCINTNFYMKGMDCMGIWVMEQYGVK 1042
            AFDL  E +  +P P+  G+     + + +L   LC+  NF+   MD   +WVM +Y   
Sbjct: 228  AFDLTHEIFTELPLPDTGGVGGGFEIDVALLGDSLCMTVNFHNSKMD---VWVMREYNRG 284

Query: 1043 ESWTKLLSVEK 1075
            +SW KL ++E+
Sbjct: 285  DSWCKLFTLEE 295


>ref|XP_003610528.1| F-box family protein [Medicago truncatula]
           gi|355511583|gb|AES92725.1| F-box family protein
           [Medicago truncatula]
          Length = 392

 Score =  132 bits (332), Expect(2) = 3e-37
 Identities = 85/220 (38%), Positives = 113/220 (51%), Gaps = 22/220 (10%)
 Frame = +1

Query: 154 MSDVPPELITDIFTRLPVKSLLRFRSVSKPYRALIDSPKFIKSHLNQSLKSKSHQSIVVS 333
           M+D+P E+ T+I +R+P K LLR RS  K +R LIDS  FI  HL     SKS  S+++ 
Sbjct: 1   MADLPTEVTTEILSRVPAKPLLRLRSTCKWWRNLIDSTDFIFLHL-----SKSRDSVIIL 55

Query: 334 LNDDTDQLYSIDFESLDKPTLLTQLGTC------VIGSFNGLLCI-KYGHRITLFNPETR 492
                 +LY +D  S+D+   L     C      V+GS NGLLCI      I  +NP  R
Sbjct: 56  RQH--SRLYELDLNSMDRVKELDHPLMCYSNRIKVLGSCNGLLCICNIADDIAFWNPTIR 113

Query: 493 MHRTVPMFYTIRP-----------CDFSVYGFGYDHVNDDYKVVWIGRVENGSTGEF--- 630
            HR +P    IR                VYGFGYD   DDYK+V I    +     F   
Sbjct: 114 KHRIIPSEPLIRKETNENNTITTLLAAHVYGFGYDSATDDYKLVSISYFVDLHNRSFDSH 173

Query: 631 -MVYSLKSDSWRKIADFPYYLCSKRVHNVFVNGALHWLVT 747
             +Y++++D W+ +   PY LC  R   VFV+GALHW+VT
Sbjct: 174 VKIYTMRTDVWKTLPSMPYALCCARTMGVFVSGALHWVVT 213



 Score = 50.4 bits (119), Expect(2) = 3e-37
 Identities = 27/70 (38%), Positives = 37/70 (52%)
 Frame = +2

Query: 869  AFDLATEEYRMVPQPEFFGMSVLMSLGVLEGKLCINTNFYMKGMDCMGIWVMEQYGVKES 1048
            AFDL  E +R V  P        M + +L G LCI  N   +G D   +WVM +YG  +S
Sbjct: 226  AFDLRFEVFREVALPGTVDGKFDMDVALLRGMLCIIEN---RGSDGFDVWVMREYGSHDS 282

Query: 1049 WTKLLSVEKP 1078
            W K+ +V +P
Sbjct: 283  WCKMFTVGQP 292


>ref|XP_003546182.1| PREDICTED: F-box protein CPR30-like [Glycine max]
          Length = 394

 Score =  133 bits (334), Expect(2) = 4e-37
 Identities = 82/224 (36%), Positives = 121/224 (54%), Gaps = 24/224 (10%)
 Frame = +1

Query: 154 MSD-VPPELITDIFTRLPVKSLLRFRSVSKPYRALIDSPKFIKSHLNQSLKSKSHQSIVV 330
           MSD +P E++T+I +RLPV+SLLRFRS SK +++LIDS      HL +SL   S+ S+++
Sbjct: 1   MSDHLPREVLTEILSRLPVRSLLRFRSTSKSWKSLIDSQHLNWLHLTRSLTLTSNTSLIL 60

Query: 331 SLNDDTDQLYSIDFESLDKPTLLTQLGTC------VIGSFNGLLCI-KYGHRITLFNPET 489
            ++ D   LY  +F +LD P  L     C      ++GS NGLLCI      I  +NP  
Sbjct: 61  RVDSD---LYQTNFPTLDPPVSLNHPLMCYSNSITLLGSCNGLLCISNVADDIAFWNPSL 117

Query: 490 RMHRTVP------------MFYTIRPCDFSVYGFGYDHVNDDYKVV----WIGRVENGST 621
           R HR +P              +  R C     GFG+DH   DYK+V    ++   +    
Sbjct: 118 RQHRILPYLPVPRRRHPDTTLFAARVC-----GFGFDHKTRDYKLVRISYFVDLHDRSFD 172

Query: 622 GEFMVYSLKSDSWRKIADFPYYLCSKRVHNVFVNGALHWLVTVK 753
            +  +Y+L++++W+ +   PY LC  R   VFV  +LHW+VT K
Sbjct: 173 SQVKLYTLRANAWKTLPSLPYALCCARTMGVFVGNSLHWVVTRK 216



 Score = 48.9 bits (115), Expect(2) = 4e-37
 Identities = 24/71 (33%), Positives = 42/71 (59%), Gaps = 2/71 (2%)
 Frame = +2

Query: 869  AFDLATEEYRMVPQPEFFGMS--VLMSLGVLEGKLCINTNFYMKGMDCMGIWVMEQYGVK 1042
            AFDL  + +R +P P+  G+     + L +L G LC+  NF+   +D   +WVM +Y  +
Sbjct: 227  AFDLTHDIFRELPLPDTGGVDGGFEIDLALLGGSLCMTVNFHKTRID---VWVMREYNRR 283

Query: 1043 ESWTKLLSVEK 1075
            +SW K+ ++E+
Sbjct: 284  DSWCKVFTLEE 294