BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00025804
         (1401 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI22917.3| unnamed protein product [Vitis vinifera]              515   e-144
ref|XP_003529542.1| PREDICTED: AMSH-like ubiquitin thiolesterase...   505   e-141
ref|XP_003550553.1| PREDICTED: AMSH-like ubiquitin thiolesterase...   499   e-139
ref|XP_002316018.1| predicted protein [Populus trichocarpa] gi|2...   490   e-136
ref|XP_003609726.1| STAM-binding protein [Medicago truncatula] g...   484   e-134

>emb|CBI22917.3| unnamed protein product [Vitis vinifera]
          Length = 516

 Score =  515 bits (1327), Expect = e-144
 Identities = 265/405 (65%), Positives = 306/405 (75%), Gaps = 3/405 (0%)
 Frame = -3

Query: 1399 SQQYIIEGTNITPYASKPSSLEWPLDRKSSLSIENKPSSSMAHLSSWKQNNSNIQAWRSN 1220
            SQQ  I+    TPY S+ SS   P+++K   S +NK + S A   SWK  N + Q   SN
Sbjct: 115  SQQQQIDVLERTPYDSEISSQWPPVNKKPFPSFDNKQAVSRAPQISWKYKNDHTQVLSSN 174

Query: 1219 --SINMQFQNLSVNLPRPKNETLSRHSFLGQTGLQSQWQGPAAEMKVHYPKYTDLASNET 1046
               ++ QFQ LS++LP PK ETLSRHSFLG  GL+ QW GP+AE+K+ YP  TDL S E 
Sbjct: 175  PMQVDKQFQKLSLSLPLPKKETLSRHSFLGPNGLRGQWLGPSAEIKIQYPSNTDLTSTEN 234

Query: 1045 SSSNQTILYDHGAVNGLPSTVNGS-SAMDSVLSLDDGRWSNPAENFFPPINDGVNNHLPL 869
              ++Q   YD   +    S + G  S M+SVLSLDDGRW  P+E       +  +++ PL
Sbjct: 235  LGTSQDEQYDLATIKD--SDLGGDRSPMESVLSLDDGRWLCPSEESPQFTIEERDDNFPL 292

Query: 868  GIIRQPSPPPVLAQVQTEHIPISPSRVADPRPGPVKPFQGGTPSSESYQHLHIPIKLMED 689
            G IRQPSPPPVLAQ+Q +  PI PS+VADPRPGP    Q G PSS SYQHLHIP+ +MED
Sbjct: 293  GNIRQPSPPPVLAQIQQDCHPIPPSKVADPRPGPATS-QHGMPSSNSYQHLHIPVSMMED 351

Query: 688  FLRLAQENTAKNLETCGVLAGSLRNRVFHITTLIIPKQKATSDSCQTLNEEELFEVQDKS 509
            FLRLA  NT KNLETCGVLAGSL+NRVFHITTLIIPKQ++TSDSCQTLNEEE+FEVQDK 
Sbjct: 352  FLRLALANTKKNLETCGVLAGSLKNRVFHITTLIIPKQESTSDSCQTLNEEEIFEVQDKL 411

Query: 508  SLFQLGWIHTHPSQTCFMSSVDLHTHYSYQVMLPEAIAIVMAPTDESSPHGIFHLSDPSG 329
            SLF LGWIHTHPSQTCFMSSVDLHTHYSYQ+MLPEAIAIVMAPTD SSPHGIFHLSDP+G
Sbjct: 412  SLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPHGIFHLSDPAG 471

Query: 328  INVMRNCQQRGFHPHEEPEDGSPIYEHCSHVYINQNLSCDVVDLR 194
            ++V+RNCQQRGFHPHEE  DGSPIYEHCSHVY+N  L  DVVDLR
Sbjct: 472  VSVIRNCQQRGFHPHEECPDGSPIYEHCSHVYMNPKLKFDVVDLR 516


>ref|XP_003529542.1| PREDICTED: AMSH-like ubiquitin thiolesterase 3-like [Glycine max]
          Length = 513

 Score =  505 bits (1301), Expect = e-141
 Identities = 260/399 (65%), Positives = 303/399 (75%), Gaps = 4/399 (1%)
 Frame = -3

Query: 1378 GTNITPYASKPSSLEWP-LDRKSSLSIEN-KPSSSMAHLSSWKQNNSNIQAWRSNSINMQ 1205
            G N    +S  SSLEWP + ++   S++  +P++  +  SSW  N  N+ +  S  I+ Q
Sbjct: 117  GFNKALQSSVNSSLEWPAVSKQVRTSLKYLQPTAGFSSHSSW--NYDNMLSLDSRPIDKQ 174

Query: 1204 FQNLSVNLPRPKNETLSRHSFLGQTGLQSQWQGPAAEMKVHYPKYTDLASNETSSSNQTI 1025
            FQ LS++LP P  ETLSRHSFLG  GL+ QW GP+AE+KV YP  +DL   + SS NQ  
Sbjct: 175  FQKLSLSLPPPNKETLSRHSFLGPNGLRGQWLGPSAEIKVQYPSSSDLTHAKDSSLNQAG 234

Query: 1024 LYDHGAV-NGLPSTVNGSSAMDSVLSLDDGRWSNPA-ENFFPPINDGVNNHLPLGIIRQP 851
            LYD GA+ +G    V  +S MDSVLSLDDG W  PA E+  P + +   + L L  I+QP
Sbjct: 235  LYDLGAIKDGDQGPV--TSTMDSVLSLDDGSWLRPAVESCSPVVTESREDPLQLLNIKQP 292

Query: 850  SPPPVLAQVQTEHIPISPSRVADPRPGPVKPFQGGTPSSESYQHLHIPIKLMEDFLRLAQ 671
             PPPVLAQV  EH PI PS+VADPRPGP K        S +YQHLHIP+K+MEDFLRLA 
Sbjct: 293  LPPPVLAQVYPEHAPIPPSKVADPRPGPAKSSHDSGLGSTTYQHLHIPVKMMEDFLRLAS 352

Query: 670  ENTAKNLETCGVLAGSLRNRVFHITTLIIPKQKATSDSCQTLNEEELFEVQDKSSLFQLG 491
            ENT KNLETCGVLAGSL+ RVFHITTLIIPKQ++TSDSCQTLNEEE+FEVQD  SLF LG
Sbjct: 353  ENTRKNLETCGVLAGSLKKRVFHITTLIIPKQESTSDSCQTLNEEEIFEVQDSLSLFPLG 412

Query: 490  WIHTHPSQTCFMSSVDLHTHYSYQVMLPEAIAIVMAPTDESSPHGIFHLSDPSGINVMRN 311
            WIHTHPSQTCFMSSVDLHTHYSYQ+MLPEAIAIVMAPTD +SPHGIFHLSDP G++V+RN
Sbjct: 413  WIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDTTSPHGIFHLSDPGGVSVIRN 472

Query: 310  CQQRGFHPHEEPEDGSPIYEHCSHVYINQNLSCDVVDLR 194
            CQQRGFHPHEEPEDG+PIYEHCSHVY+N NL  DVVDLR
Sbjct: 473  CQQRGFHPHEEPEDGTPIYEHCSHVYMNANLKFDVVDLR 511


>ref|XP_003550553.1| PREDICTED: AMSH-like ubiquitin thiolesterase 3-like [Glycine max]
          Length = 504

 Score =  499 bits (1285), Expect = e-139
 Identities = 253/398 (63%), Positives = 299/398 (75%), Gaps = 3/398 (0%)
 Frame = -3

Query: 1378 GTNITPYASKPSSLEWPLDRKSSLSIENKPSSSMAHLSSWKQNNS-NIQAWRSNSINMQF 1202
            G N    +S  SSL+WP       ++  +P++  +  SSW  +N  ++ +W    I+ QF
Sbjct: 117  GFNKALQSSVNSSLQWP-------AVNKQPAAGFSSQSSWNYDNMLSLDSW---PIDKQF 166

Query: 1201 QNLSVNLPRPKNETLSRHSFLGQTGLQSQWQGPAAEMKVHYPKYTDLASNETSSSNQTIL 1022
            Q LSV+LP P  ETLSRHSFLG  GL+ QW GP+AE+KV YP   +L   + SS NQ  L
Sbjct: 167  QKLSVSLPPPNKETLSRHSFLGPNGLRGQWLGPSAEIKVQYPSSGELTQAKDSSPNQAGL 226

Query: 1021 YDHGAV-NGLPSTVNGSSAMDSVLSLDDGRWSNPA-ENFFPPINDGVNNHLPLGIIRQPS 848
            YD  A+ +G    V  +S MDSVLSLDDGRW  PA E+  P + +   + L L  I+QP 
Sbjct: 227  YDLVAIKDGDQGPV--TSTMDSVLSLDDGRWLRPAVESCSPVVTESREDPLQLLNIKQPL 284

Query: 847  PPPVLAQVQTEHIPISPSRVADPRPGPVKPFQGGTPSSESYQHLHIPIKLMEDFLRLAQE 668
            PPP+LAQV  E +PI PS+VADPRPGP K        + +YQHLHIP+K+ME+FLRLA E
Sbjct: 285  PPPILAQVYPERVPIPPSKVADPRPGPAKSSHDSRLDATTYQHLHIPVKMMEEFLRLASE 344

Query: 667  NTAKNLETCGVLAGSLRNRVFHITTLIIPKQKATSDSCQTLNEEELFEVQDKSSLFQLGW 488
            NT KNLETCGVLAGSL+ RVFHITTLIIPKQ++TSDSCQTLNEEE+FEVQD  SLF LGW
Sbjct: 345  NTRKNLETCGVLAGSLKKRVFHITTLIIPKQESTSDSCQTLNEEEIFEVQDSLSLFPLGW 404

Query: 487  IHTHPSQTCFMSSVDLHTHYSYQVMLPEAIAIVMAPTDESSPHGIFHLSDPSGINVMRNC 308
            IHTHPSQTCFMSSVDLHTHYSYQ+MLPEAIAIVMAPTD +SPHGIFHLSDP G++V+RNC
Sbjct: 405  IHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDTTSPHGIFHLSDPGGVSVIRNC 464

Query: 307  QQRGFHPHEEPEDGSPIYEHCSHVYINQNLSCDVVDLR 194
            QQRGFHPHEEPEDG+PIYEHCSHVY+N NL  DVVDLR
Sbjct: 465  QQRGFHPHEEPEDGTPIYEHCSHVYMNANLKFDVVDLR 502


>ref|XP_002316018.1| predicted protein [Populus trichocarpa] gi|222865058|gb|EEF02189.1|
            predicted protein [Populus trichocarpa]
          Length = 470

 Score =  490 bits (1262), Expect = e-136
 Identities = 245/347 (70%), Positives = 270/347 (77%), Gaps = 1/347 (0%)
 Frame = -3

Query: 1231 WRSNSINMQFQNLSVNLPRPKNETLSRHSFLGQTGLQSQWQGPAAEMKVHYPKYTDLASN 1052
            W   + N     LSV+LP PK ETLSRHSFLG  G + QW GP+AE+KV YP   DL S 
Sbjct: 125  WPPANKNSYSSKLSVSLPLPKQETLSRHSFLGPNGFRGQWSGPSAEIKVQYPSNADLTST 184

Query: 1051 ETSSSNQTILYDHGAVNGLPSTVNGSSAMDSVLSLDDGRWSNPAENFFPP-INDGVNNHL 875
            E SS NQ   YD  AV    S     S M+SVLSLDDGRW   AE+  P  IN+   +  
Sbjct: 185  ENSSLNQAGQYDAMAVKDANSAAV-ESTMESVLSLDDGRWPRLAEDTCPTLINEAREDPF 243

Query: 874  PLGIIRQPSPPPVLAQVQTEHIPISPSRVADPRPGPVKPFQGGTPSSESYQHLHIPIKLM 695
                IRQPSPPPVLA+VQ E  PI  S+VADPRPGP KP   G PSS SYQHLH+P+ LM
Sbjct: 244  QFVGIRQPSPPPVLAKVQQESTPIPSSKVADPRPGPAKPSDDGLPSSNSYQHLHVPVNLM 303

Query: 694  EDFLRLAQENTAKNLETCGVLAGSLRNRVFHITTLIIPKQKATSDSCQTLNEEELFEVQD 515
            EDFLRLA+ NT KNLETCGVLAGSL+N+VFHITTLIIPKQ++TSDSCQTLNEEE+FEVQD
Sbjct: 304  EDFLRLARANTEKNLETCGVLAGSLKNKVFHITTLIIPKQESTSDSCQTLNEEEIFEVQD 363

Query: 514  KSSLFQLGWIHTHPSQTCFMSSVDLHTHYSYQVMLPEAIAIVMAPTDESSPHGIFHLSDP 335
            K SLF LGWIHTHPSQTCFMSSVDLHTHYSYQ+MLPEAIAIVMAPTDESSPHGIFHLSDP
Sbjct: 364  KLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPHGIFHLSDP 423

Query: 334  SGINVMRNCQQRGFHPHEEPEDGSPIYEHCSHVYINQNLSCDVVDLR 194
            SG++V+RNCQQRGFHPHEE  DGSPIYEHCSHVY+N  +  DVVDLR
Sbjct: 424  SGVSVIRNCQQRGFHPHEESLDGSPIYEHCSHVYMNSIMKFDVVDLR 470


>ref|XP_003609726.1| STAM-binding protein [Medicago truncatula]
            gi|355510781|gb|AES91923.1| STAM-binding protein
            [Medicago truncatula]
          Length = 509

 Score =  484 bits (1246), Expect = e-134
 Identities = 247/399 (61%), Positives = 297/399 (74%), Gaps = 4/399 (1%)
 Frame = -3

Query: 1378 GTNITPYASKPSSLEWP-LDRKSSLSIENKPSSSMAHLSSWKQNNSNIQAWRSNSINMQF 1202
            G ++   +S  SSLEWP ++++ + S++ K S+ +   SSWK NN+   ++ S  I+ QF
Sbjct: 117  GFDMALQSSANSSLEWPAVNKRYNSSMDFKQSTGLGSQSSWKPNNT--LSYNSMPIDKQF 174

Query: 1201 QNLSVNLPRPKNETLSRHSFLGQTGLQSQWQGPAAEMKVHYPKYTDLASNETSSSNQTIL 1022
            Q LS+N+P P  ETLSRHSFLG  GL+ QW GP+AE+KV YP   DL   + SS   T+ 
Sbjct: 175  QKLSLNVPLPNKETLSRHSFLGPNGLRGQWLGPSAEIKVQYPSSNDLTHAKDSS---TVK 231

Query: 1021 YDHGAVNGLPSTVNG--SSAMDSVLSLDDGRWSNP-AENFFPPINDGVNNHLPLGIIRQP 851
            YD   + G+     G  +S MDS+LSLDDGRW +P AE+  P + +   + L    I+QP
Sbjct: 232  YD---LAGIKDGDQGPLTSTMDSILSLDDGRWLHPTAESCSPVVTEPREDFLQSLNIKQP 288

Query: 850  SPPPVLAQVQTEHIPISPSRVADPRPGPVKPFQGGTPSSESYQHLHIPIKLMEDFLRLAQ 671
             PPPVLAQV  E   I  S+VADPRPGP K          ++QHLHIP+K+MEDFLRLA 
Sbjct: 289  LPPPVLAQVHPERACIPHSKVADPRPGPAKSSHDSGHGPTTFQHLHIPVKMMEDFLRLAS 348

Query: 670  ENTAKNLETCGVLAGSLRNRVFHITTLIIPKQKATSDSCQTLNEEELFEVQDKSSLFQLG 491
             NT KNLETCGVLAGSL+NRVF ITTLIIPKQ++TSDSCQTLNEEE+FEVQD  SLF LG
Sbjct: 349  VNTRKNLETCGVLAGSLKNRVFQITTLIIPKQESTSDSCQTLNEEEIFEVQDSLSLFSLG 408

Query: 490  WIHTHPSQTCFMSSVDLHTHYSYQVMLPEAIAIVMAPTDESSPHGIFHLSDPSGINVMRN 311
            WIHTHPSQTCFMSSVDLHTHYSYQ+MLPEAIAIVMAPTD  SPHGIFHLSDP G++V+RN
Sbjct: 409  WIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDTESPHGIFHLSDPGGVSVIRN 468

Query: 310  CQQRGFHPHEEPEDGSPIYEHCSHVYINQNLSCDVVDLR 194
            CQ+RGFHPHEEP DGSPIYEHCSHVY+N N+  DV+DLR
Sbjct: 469  CQERGFHPHEEPSDGSPIYEHCSHVYMNANMKFDVLDLR 507