BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00025559
         (748 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAN82223.1| hypothetical protein VITISV_011871 [Vitis vinifera]   290   2e-76
ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   290   3e-76
ref|XP_002308030.1| predicted protein [Populus trichocarpa] gi|2...   267   2e-69
ref|XP_003521174.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   263   4e-68
ref|NP_567993.1| cytoplasmic tRNA 2-thiolation protein 2 [Arabid...   260   2e-67

>emb|CAN82223.1| hypothetical protein VITISV_011871 [Vitis vinifera]
          Length = 747

 Score =  290 bits (742), Expect = 2e-76
 Identities = 158/248 (63%), Positives = 179/248 (72%), Gaps = 15/248 (6%)
 Frame = -1

Query: 700 MSCNGTGCDSGCYKDEQD------KELRNGVLPRVTE------HRICLKCKXXXXXXXXX 557
           M+C    C SGC++D+ D      K+L N  L   ++      H +CLKCK         
Sbjct: 1   MACGAGNCQSGCFRDDDDSVVTEKKKLTNRTLSSGSDIDLPNHHNLCLKCKVNETLASSG 60

Query: 556 XXXXXSN---FCADCFRTNLFGKFKFAVTSNAMISPSDNVLVAFSGGVSSRVALQFVHEM 386
                 +   FC DCFR NLFGKFK AVTSNAMISP+DNVLVAFSGG SSRVALQFVHEM
Sbjct: 61  TVSVDGDIGRFCFDCFRGNLFGKFKLAVTSNAMISPTDNVLVAFSGGPSSRVALQFVHEM 120

Query: 385 QHKAQKNFDASRDRSLPVFGVGVAFIDELAIHPVPLHEFDESIEAMKLIVDGLAPPRKAF 206
           QHKAQKNFDASRDRSLPVFGVGVAF+DE A+  +P  EFD++I+ MK IV  LAPP KAF
Sbjct: 121 QHKAQKNFDASRDRSLPVFGVGVAFVDESAVSRIPSDEFDKAIQDMKSIVSNLAPPXKAF 180

Query: 205 HVVPIESIFPLDFNGRKDNLKELLSAVSDKTGKEDLLVQLRMLSLQKFALENGYTKLVLG 26
           HVVPIES++    NGR D L+ELL+AV D TGKEDLL  LRMLSLQK A ENGYTKL+LG
Sbjct: 181 HVVPIESVY--SSNGR-DRLEELLNAVKDVTGKEDLLQHLRMLSLQKNAYENGYTKLLLG 237

Query: 25  SCTSRIAC 2
           SCTSRIAC
Sbjct: 238 SCTSRIAC 245


>ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Vitis vinifera]
           gi|297739656|emb|CBI29838.3| unnamed protein product
           [Vitis vinifera]
          Length = 468

 Score =  290 bits (741), Expect = 3e-76
 Identities = 158/248 (63%), Positives = 179/248 (72%), Gaps = 15/248 (6%)
 Frame = -1

Query: 700 MSCNGTGCDSGCYKDEQD------KELRNGVLPRVTE------HRICLKCKXXXXXXXXX 557
           M+C    C SGC++D+ D      K+L N  L   ++      H +CLKCK         
Sbjct: 1   MACGAGNCQSGCFRDDDDSVVTEKKKLTNRTLSSGSDIDLPNHHNLCLKCKVNETLASSG 60

Query: 556 XXXXXSN---FCADCFRTNLFGKFKFAVTSNAMISPSDNVLVAFSGGVSSRVALQFVHEM 386
                 +   FC DCFR NLFGKFK AVTSNAMISP+DNVLVAFSGG SSRVALQFVHEM
Sbjct: 61  TVSVDGDIGRFCFDCFRGNLFGKFKLAVTSNAMISPTDNVLVAFSGGPSSRVALQFVHEM 120

Query: 385 QHKAQKNFDASRDRSLPVFGVGVAFIDELAIHPVPLHEFDESIEAMKLIVDGLAPPRKAF 206
           QHKAQKNFDASRDRSLPVFGVGVAF+DE A+  +P  EFD++I+ MK IV  LAPP KAF
Sbjct: 121 QHKAQKNFDASRDRSLPVFGVGVAFVDESAVSRIPSDEFDKAIQDMKSIVSNLAPPDKAF 180

Query: 205 HVVPIESIFPLDFNGRKDNLKELLSAVSDKTGKEDLLVQLRMLSLQKFALENGYTKLVLG 26
           HVVPIES++    NGR D L+ELL+AV D TGKEDLL  LRMLSLQK A ENGYTKL+LG
Sbjct: 181 HVVPIESVY--SSNGR-DRLEELLNAVKDVTGKEDLLQHLRMLSLQKNAYENGYTKLLLG 237

Query: 25  SCTSRIAC 2
           SCTSRIAC
Sbjct: 238 SCTSRIAC 245


>ref|XP_002308030.1| predicted protein [Populus trichocarpa] gi|222854006|gb|EEE91553.1|
           predicted protein [Populus trichocarpa]
          Length = 443

 Score =  267 bits (683), Expect = 2e-69
 Identities = 136/237 (57%), Positives = 168/237 (70%), Gaps = 4/237 (1%)
 Frame = -1

Query: 700 MSCNGTGCDSGCYKDEQDKELRNGVLPRVT----EHRICLKCKXXXXXXXXXXXXXXSNF 533
           MSCN +GC S CYK+           P +      + +CLKCK              + F
Sbjct: 1   MSCNSSGCQSNCYKNGDQVSDSPPPPPTLAAANNSNNLCLKCKTNSPITPGSLSGDEARF 60

Query: 532 CADCFRTNLFGKFKFAVTSNAMISPSDNVLVAFSGGVSSRVALQFVHEMQHKAQKNFDAS 353
           CADCFR+N++GKF+ AVTS AMISPSDNVLVAFSGG SSRVALQFVH +Q +AQKNF+AS
Sbjct: 61  CADCFRSNIYGKFRLAVTSQAMISPSDNVLVAFSGGPSSRVALQFVHGLQDRAQKNFEAS 120

Query: 352 RDRSLPVFGVGVAFIDELAIHPVPLHEFDESIEAMKLIVDGLAPPRKAFHVVPIESIFPL 173
           +D+SLPVFGVGVAFIDE A++PV   + D +I+ M+ +V  LAPP K  HVVPIE+I+  
Sbjct: 121 KDKSLPVFGVGVAFIDESAVYPVSSDKVDGAIQDMRSVVSNLAPPTKQLHVVPIENIYAS 180

Query: 172 DFNGRKDNLKELLSAVSDKTGKEDLLVQLRMLSLQKFALENGYTKLVLGSCTSRIAC 2
           D +  KD L +LL AV+D TGKEDLL+ LRML+LQ  A +NGY +L+LGSCTSRIAC
Sbjct: 181 DSSDGKDRLTKLLDAVNDATGKEDLLLHLRMLALQMVASQNGYNRLILGSCTSRIAC 237


>ref|XP_003521174.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Glycine
           max]
          Length = 458

 Score =  263 bits (671), Expect = 4e-68
 Identities = 131/237 (55%), Positives = 170/237 (71%), Gaps = 4/237 (1%)
 Frame = -1

Query: 700 MSCNGTGCDSG-CYKDEQ---DKELRNGVLPRVTEHRICLKCKXXXXXXXXXXXXXXSNF 533
           M+CNG+GC SG CYKDE     +  ++          +C+KCK                F
Sbjct: 1   MACNGSGCQSGGCYKDEDATCKQPPKSEEADSKNPSNVCIKCKLNDAVSGYGGIDDG-RF 59

Query: 532 CADCFRTNLFGKFKFAVTSNAMISPSDNVLVAFSGGVSSRVALQFVHEMQHKAQKNFDAS 353
           CADCF+TNLFGKF+FAVTSNAMI+P+D VLVAFSGG SSRVALQFVH+MQ +AQ+NFDAS
Sbjct: 60  CADCFKTNLFGKFRFAVTSNAMITPTDKVLVAFSGGPSSRVALQFVHDMQERAQRNFDAS 119

Query: 352 RDRSLPVFGVGVAFIDELAIHPVPLHEFDESIEAMKLIVDGLAPPRKAFHVVPIESIFPL 173
           RDRSLPVFGVGV FIDE A+ P+P +E +E++E +  +V  LAPPRK  H+VPIE+++  
Sbjct: 120 RDRSLPVFGVGVVFIDESAVLPIPSNEMEEAVEVVSEVVSSLAPPRKELHIVPIETVYSS 179

Query: 172 DFNGRKDNLKELLSAVSDKTGKEDLLVQLRMLSLQKFALENGYTKLVLGSCTSRIAC 2
           D    K+ + ++++ V D TG+ED+L+ LRML+LQK A E GY +++LGSC SRIAC
Sbjct: 180 DSGDGKERITKVVNTVIDPTGREDVLLCLRMLALQKVASEFGYNRIILGSCISRIAC 236


>ref|NP_567993.1| cytoplasmic tRNA 2-thiolation protein 2 [Arabidopsis thaliana]
           gi|332278212|sp|O65628.3|CTU2_ARATH RecName:
           Full=Cytoplasmic tRNA 2-thiolation protein 2
           gi|115646788|gb|ABJ17118.1| At4g35910 [Arabidopsis
           thaliana] gi|332661188|gb|AEE86588.1| cytoplasmic tRNA
           2-thiolation protein 2 [Arabidopsis thaliana]
          Length = 458

 Score =  260 bits (664), Expect = 2e-67
 Identities = 136/235 (57%), Positives = 165/235 (70%), Gaps = 3/235 (1%)
 Frame = -1

Query: 700 MSCNGTGCDSGCYKDEQD---KELRNGVLPRVTEHRICLKCKXXXXXXXXXXXXXXSNFC 530
           M+CN +GC+SGCY  E+D   K + + V        +C+KCK                FC
Sbjct: 1   MACNSSGCESGCYDREKDNGSKIVDDAVSGGGNHESVCVKCKCNAPMTFGDGGFDDGRFC 60

Query: 529 ADCFRTNLFGKFKFAVTSNAMISPSDNVLVAFSGGVSSRVALQFVHEMQHKAQKNFDASR 350
           ADCFR N+FGKF+ AVTS+AMI+PSDNVLVAFSGG SSRV+LQFVHE+Q KA KN++ASR
Sbjct: 61  ADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQFVHELQIKALKNYEASR 120

Query: 349 DRSLPVFGVGVAFIDELAIHPVPLHEFDESIEAMKLIVDGLAPPRKAFHVVPIESIFPLD 170
           DRSLPVFGVGVAF+DE A  P    E  ++IE ++  V  L+PP K  HVVP+ESIF  D
Sbjct: 121 DRSLPVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSPPAKDLHVVPVESIFGSD 180

Query: 169 FNGRKDNLKELLSAVSDKTGKEDLLVQLRMLSLQKFALENGYTKLVLGSCTSRIA 5
               +D L +LL +V D TGKEDLL+ L+MLSLQK A ENGY +LVLGSCTSRIA
Sbjct: 181 SLDARDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKVAAENGYNRLVLGSCTSRIA 235