BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00025547
         (1070 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002279531.1| PREDICTED: cytochrome P450 86B1 [Vitis vinif...   395   e-108
emb|CAN80536.1| hypothetical protein VITISV_035975 [Vitis vinifera]   395   e-108
ref|XP_002279670.1| PREDICTED: cytochrome P450 86B1 [Vitis vinif...   394   e-107
ref|XP_002310605.1| cytochrome P450 [Populus trichocarpa] gi|222...   394   e-107
ref|XP_002275905.1| PREDICTED: cytochrome P450 86B1 [Vitis vinif...   389   e-106

>ref|XP_002279531.1| PREDICTED: cytochrome P450 86B1 [Vitis vinifera]
          Length = 507

 Score =  395 bits (1016), Expect = e-108
 Identities = 189/334 (56%), Positives = 238/334 (71%), Gaps = 1/334 (0%)
 Frame = -1

Query: 1070 LSFDTTCMFVTGYDPMSLSTEFPHVPFSKAMDEAEDGIFARHIMPQSMWKLLRWLNIGSE 891
            L+FDTTCM VTG+D   LS EFP VPF+ A+++ E+ +F RH +P+++W+LLRWL IG E
Sbjct: 175  LTFDTTCMLVTGFDTKCLSIEFPEVPFATALEDVEEAVFYRHALPETLWRLLRWLGIGKE 234

Query: 890  KKMKEAQKTFDRVLYTYISRKRIELRSKEVKSGEDKEKEGVXXXXXXXXXXXXXXXLKND 711
            KK+++  KT D  +  YIS KR EL     K  ED++   +                K D
Sbjct: 235  KKLRKGWKTLDHTIAEYISMKREELSKGITKLREDEDGADLLTSYMTEDNAIGL---KCD 291

Query: 710  DKFVRDIVLNHMLAGRDTTSSALTWFLWLVMTNPHVEANIREELKSKIPENEAQK-RRIF 534
             KF+RD +LN M+AGRDTTSSALTWF WLV  NP VE+ IREE+++ IPE E +K R IF
Sbjct: 292  GKFLRDTILNFMIAGRDTTSSALTWFFWLVSKNPLVESKIREEIETAIPEKEDRKGRHIF 351

Query: 533  SEEELRQVPYLHGALSEALRLYPPVPFQHKEATKSDILPSGHHVHPNLRVMFPLYSMGRM 354
              E L ++ YLH A+ EALRLYPPVPFQHK   + D+LPSGH V P +++ FP+Y+MGRM
Sbjct: 352  KTEGLNKLVYLHAAMCEALRLYPPVPFQHKAPIQPDVLPSGHKVDPKMKIFFPVYAMGRM 411

Query: 353  ADVWGKDCWEYKPERWISEDGSIKHVPSHKFVSFNAGPRTCLGKNVAYSQMKAVAAALIH 174
              +WGKDC E+KPERWISE G IKH PS+KF SFNAGPRTC+GK VA++QMKAVAA +IH
Sbjct: 412  TAIWGKDCLEFKPERWISELGKIKHEPSYKFFSFNAGPRTCIGKEVAFTQMKAVAATIIH 471

Query: 173  NYSFELEEGHVVTPTLSLILYMKHGLKVRVKNRW 72
            NY  E+ +GH V P +S+ILYMK G KVRV  RW
Sbjct: 472  NYHVEMVKGHHVAPNVSIILYMKRGFKVRVSKRW 505


>emb|CAN80536.1| hypothetical protein VITISV_035975 [Vitis vinifera]
          Length = 471

 Score =  395 bits (1016), Expect = e-108
 Identities = 189/334 (56%), Positives = 238/334 (71%), Gaps = 1/334 (0%)
 Frame = -1

Query: 1070 LSFDTTCMFVTGYDPMSLSTEFPHVPFSKAMDEAEDGIFARHIMPQSMWKLLRWLNIGSE 891
            L+FDTTCM VTG+D   LS EFP VPF+ A+++ E+ +F RH +P+++W+LLRWL IG E
Sbjct: 139  LTFDTTCMLVTGFDTKCLSIEFPEVPFATALEDVEEAVFYRHALPETLWRLLRWLGIGKE 198

Query: 890  KKMKEAQKTFDRVLYTYISRKRIELRSKEVKSGEDKEKEGVXXXXXXXXXXXXXXXLKND 711
            KK+++  KT D  +  YIS KR EL     K  ED++   +                K D
Sbjct: 199  KKLRKGWKTLDHTIAEYISMKREELSKGITKLREDEDGADLLTSYMTEDNAIGL---KCD 255

Query: 710  DKFVRDIVLNHMLAGRDTTSSALTWFLWLVMTNPHVEANIREELKSKIPENEAQK-RRIF 534
             KF+RD +LN M+AGRDTTSSALTWF WLV  NP VE+ IREE+++ IPE E +K R IF
Sbjct: 256  GKFLRDTILNFMIAGRDTTSSALTWFFWLVSKNPLVESKIREEIETAIPEKEDRKGRHIF 315

Query: 533  SEEELRQVPYLHGALSEALRLYPPVPFQHKEATKSDILPSGHHVHPNLRVMFPLYSMGRM 354
              E L ++ YLH A+ EALRLYPPVPFQHK   + D+LPSGH V P +++ FP+Y+MGRM
Sbjct: 316  KTEGLNKLVYLHAAMCEALRLYPPVPFQHKAPIQPDVLPSGHKVDPKMKIFFPVYAMGRM 375

Query: 353  ADVWGKDCWEYKPERWISEDGSIKHVPSHKFVSFNAGPRTCLGKNVAYSQMKAVAAALIH 174
              +WGKDC E+KPERWISE G IKH PS+KF SFNAGPRTC+GK VA++QMKAVAA +IH
Sbjct: 376  TAIWGKDCLEFKPERWISELGKIKHEPSYKFFSFNAGPRTCIGKEVAFTQMKAVAATIIH 435

Query: 173  NYSFELEEGHVVTPTLSLILYMKHGLKVRVKNRW 72
            NY  E+ +GH V P +S+ILYMK G KVRV  RW
Sbjct: 436  NYHVEMVKGHHVAPNVSIILYMKRGFKVRVSKRW 469


>ref|XP_002279670.1| PREDICTED: cytochrome P450 86B1 [Vitis vinifera]
          Length = 502

 Score =  394 bits (1012), Expect = e-107
 Identities = 189/332 (56%), Positives = 238/332 (71%)
 Frame = -1

Query: 1067 SFDTTCMFVTGYDPMSLSTEFPHVPFSKAMDEAEDGIFARHIMPQSMWKLLRWLNIGSEK 888
            +FDTTC  +TG+D   LS EFP V F+KAMD+ E+ IF RH++P+S+WKL RWL IG EK
Sbjct: 176  TFDTTCKLITGFDTKCLSIEFPRVLFAKAMDDVEEAIFFRHVVPESLWKLQRWLGIGEEK 235

Query: 887  KMKEAQKTFDRVLYTYISRKRIELRSKEVKSGEDKEKEGVXXXXXXXXXXXXXXXLKNDD 708
            K+ +  +T D V+  YIS K  EL     K  ED+E   +                   +
Sbjct: 236  KLSKGWETIDNVIAEYISMKHEELSKGIAKLQEDEEGTDLLTSYMNEDSTMEL----KSN 291

Query: 707  KFVRDIVLNHMLAGRDTTSSALTWFLWLVMTNPHVEANIREELKSKIPENEAQKRRIFSE 528
            KF+RD ++N +LAGRDTTS+ALTWF WLV  NP VEA IREELK  IPE + QK  IF  
Sbjct: 292  KFLRDTIVNFLLAGRDTTSAALTWFFWLVSKNPLVEAKIREELKETIPEKD-QKWHIFRT 350

Query: 527  EELRQVPYLHGALSEALRLYPPVPFQHKEATKSDILPSGHHVHPNLRVMFPLYSMGRMAD 348
            EEL ++ YLHGAL E LRLYPPVPFQHK   ++D+LPSGH VHP ++++F LY+MGRM  
Sbjct: 351  EELNKLVYLHGALCETLRLYPPVPFQHKAPHQADVLPSGHRVHPKMKILFSLYAMGRMTT 410

Query: 347  VWGKDCWEYKPERWISEDGSIKHVPSHKFVSFNAGPRTCLGKNVAYSQMKAVAAALIHNY 168
            +WGKDC+E+KPERWI+E G IK+ PS+KF +FNAGPRTCLGK VA++QMKAVAAA+IHNY
Sbjct: 411  IWGKDCFEFKPERWITEQGGIKYEPSYKFFAFNAGPRTCLGKGVAFTQMKAVAAAIIHNY 470

Query: 167  SFELEEGHVVTPTLSLILYMKHGLKVRVKNRW 72
            + +L +GH V P +S+IL+MKH L VRV  RW
Sbjct: 471  NVQLVQGHPVAPNVSIILHMKHELMVRVTKRW 502


>ref|XP_002310605.1| cytochrome P450 [Populus trichocarpa] gi|222853508|gb|EEE91055.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 505

 Score =  394 bits (1011), Expect = e-107
 Identities = 193/332 (58%), Positives = 240/332 (72%)
 Frame = -1

Query: 1067 SFDTTCMFVTGYDPMSLSTEFPHVPFSKAMDEAEDGIFARHIMPQSMWKLLRWLNIGSEK 888
            +FD T + VTGYDP  LS EFP V FSKAMD+AE+ IF RH+ P+   KL RWL  G E+
Sbjct: 177  TFDITSILVTGYDPGCLSIEFPQVEFSKAMDDAEEAIFYRHLWPEIFLKLQRWLGFGQEQ 236

Query: 887  KMKEAQKTFDRVLYTYISRKRIELRSKEVKSGEDKEKEGVXXXXXXXXXXXXXXXLKNDD 708
            K+KEA +T D ++  YISRKR +L SK+  + ED++  GV                K DD
Sbjct: 237  KLKEAWQTLDHMVAEYISRKREKL-SKKTTTIEDED--GVDLLTSYMSHPEIMGL-KTDD 292

Query: 707  KFVRDIVLNHMLAGRDTTSSALTWFLWLVMTNPHVEANIREELKSKIPENEAQKRRIFSE 528
            KF+RD ++N  LAGRDTTSSALTWF WLV  NP VE+ IR+ELK+ +P  E +  R+FS 
Sbjct: 293  KFLRDTIVNFFLAGRDTTSSALTWFFWLVSQNPQVESKIRQELKATMPAEERENWRLFST 352

Query: 527  EELRQVPYLHGALSEALRLYPPVPFQHKEATKSDILPSGHHVHPNLRVMFPLYSMGRMAD 348
            +EL  + YLHGAL E LRLYPPVPFQHKE  KSD+LPSGH V PN+ V+F +Y MGRM  
Sbjct: 353  QELSNLVYLHGALCETLRLYPPVPFQHKEPLKSDVLPSGHRVGPNMMVLFSVYVMGRMTS 412

Query: 347  VWGKDCWEYKPERWISEDGSIKHVPSHKFVSFNAGPRTCLGKNVAYSQMKAVAAALIHNY 168
            +WG DC E+KPERWIS+ G IKH PS+KF++FNAGPRTCLG+ VA++QMKAVAAA+IHNY
Sbjct: 413  IWGPDCLEFKPERWISDSGKIKHEPSYKFLAFNAGPRTCLGRGVAFTQMKAVAAAIIHNY 472

Query: 167  SFELEEGHVVTPTLSLILYMKHGLKVRVKNRW 72
               + EGH V P+ S+IL+MKHGLKV +  +W
Sbjct: 473  QVHVVEGHPVAPSSSIILHMKHGLKVSLSRKW 504


>ref|XP_002275905.1| PREDICTED: cytochrome P450 86B1 [Vitis vinifera]
          Length = 507

 Score =  389 bits (999), Expect = e-106
 Identities = 190/334 (56%), Positives = 237/334 (70%), Gaps = 1/334 (0%)
 Frame = -1

Query: 1070 LSFDTTCMFVTGYDPMSLSTEFPHVPFSKAMDEAEDGIFARHIMPQSMWKLLRWLNIGSE 891
            L+FDTTCM VTG+D   LS E P VPF+ A+++AE+ I  RH++P+++W+L RWL IG E
Sbjct: 176  LTFDTTCMLVTGFDSKCLSIELPEVPFATALEDAEEAILHRHVLPETLWRLQRWLGIGKE 235

Query: 890  KKMKEAQKTFDRVLYTYISRKRIELRSKEVKSGEDKEKEGVXXXXXXXXXXXXXXXLKND 711
            KK ++A +T D  +  YIS+KR EL    +   E  E++GV                K D
Sbjct: 236  KKHQKAWETLDHTIAEYISKKRDELSKGIINLQE--EEDGVDLLTSYMSEDITMGL-KCD 292

Query: 710  DKFVRDIVLNHMLAGRDTTSSALTWFLWLVMTNPHVEANIREELKSKIPENEAQK-RRIF 534
             KF+RD +LN M+AGRDTTSSALTWF WLV  NP VE+ IREEL+S IPE E +K R  F
Sbjct: 293  HKFLRDTILNFMIAGRDTTSSALTWFFWLVSKNPLVESKIREELESAIPEKEDKKGRHTF 352

Query: 533  SEEELRQVPYLHGALSEALRLYPPVPFQHKEATKSDILPSGHHVHPNLRVMFPLYSMGRM 354
              E L ++ YLH AL E LRLYPPVPFQHK   + D+LPSGH V P ++++F LY+MGRM
Sbjct: 353  KTEALNKLVYLHAALCETLRLYPPVPFQHKAPLQPDMLPSGHRVDPKMKILFSLYAMGRM 412

Query: 353  ADVWGKDCWEYKPERWISEDGSIKHVPSHKFVSFNAGPRTCLGKNVAYSQMKAVAAALIH 174
              +WG DC E+KPERWISE G IKH PS+KF +FNAGPRTCLGK VA++QMKAVAA +IH
Sbjct: 413  TSIWGTDCLEFKPERWISERGRIKHEPSYKFFAFNAGPRTCLGKEVAFTQMKAVAATIIH 472

Query: 173  NYSFELEEGHVVTPTLSLILYMKHGLKVRVKNRW 72
            NY  ++ EGH V P +S+ILYMKHGL VRV  RW
Sbjct: 473  NYHVQVVEGHHVAPNVSIILYMKHGLMVRVSKRW 506