BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00024797
         (1310 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002283306.1| PREDICTED: putative E3 ubiquitin-protein lig...   451   e-124
emb|CAN71223.1| hypothetical protein VITISV_011730 [Vitis vinifera]   451   e-124
emb|CBI16457.3| unnamed protein product [Vitis vinifera]              441   e-121
ref|XP_002304021.1| predicted protein [Populus trichocarpa] gi|2...   415   e-113
gb|ACJ02343.1| U-box domain-containing protein [Helianthus annuus]    340   5e-91

>ref|XP_002283306.1| PREDICTED: putative E3 ubiquitin-protein ligase LIN-1-like [Vitis
            vinifera]
          Length = 1148

 Score =  451 bits (1161), Expect = e-124
 Identities = 237/433 (54%), Positives = 305/433 (70%), Gaps = 3/433 (0%)
 Frame = +3

Query: 21   NPMENHSWSFHQDRSGNSDYFVERPSFLCIPEDFICPLSGLLFEEPVTLETGQTFETEAI 200
            +P + +  S  +D  GN  YF E   F  IP+DFICPL+G LFE+PVT+ETGQTFE  AI
Sbjct: 643  DPNQTNMESSAKDLHGNCQYFNEGSFFSSIPQDFICPLTGRLFEDPVTIETGQTFERHAI 702

Query: 201  RNWFYKGHRTCPISGVTLEYQAVPLTNFILKRVVDTWKFEHCKTILGFASQLAGNTEEHS 380
            R WF +G+R CP++G  LE   VPLTNFILKRV+D WK E+C+ +L FAS++ G++ EH 
Sbjct: 703  REWFNQGNRNCPVTGKALEGLGVPLTNFILKRVIDGWKSENCRHLLAFASKVEGSSGEHG 762

Query: 381  -NFKDEAAVCIFEHLLTFFSRDERIKKAKLLISLGGLQFLVQRFKCGKLEEKTVIAALLC 557
               KDE A+   E  L+  S++E++  AK LISLGGLQFL +RF+ G LEEKT +AAL+C
Sbjct: 763  VEPKDETAIYALEQFLSGSSKEEKLTNAKHLISLGGLQFLTRRFELGNLEEKTCVAALMC 822

Query: 558  QCIEADSGCRNYIARNIEKICFIELLHCKQAKSIANAVSLLTELICLNRRRDVCSFLRGF 737
             CIEAD  C+N IA+ I+K C +ELLH KQAKS  NAV LLTELIC++R +DV  FL  F
Sbjct: 823  SCIEADYRCKNEIAKYIKKPCLLELLHSKQAKSRTNAVLLLTELICMHRWKDVTLFLSSF 882

Query: 738  ENEEMVNTMHVILIYLQSCPSEQRPXXXXXXXXXXXXXAPKKYSIYREEAIDAITVALES 917
            +NE +++ MHV+L+YLQS   EQRP              P+KYSIYREEA+DAI VALE 
Sbjct: 883  QNEGIMSAMHVLLVYLQSSSPEQRPLVAVLLLHLDLLVEPRKYSIYREEAVDAIVVALEG 942

Query: 918  SLTDDKIREKCCHALLVLGGRFSLSGKVMVEDWILKKAGFIDCHELELVDSEE-GIIPLN 1094
            SLTD+ +REKCC ALL+L G FS SG V  E WILK AG +D H+L   ++EE G++   
Sbjct: 943  SLTDENVREKCCRALLILTGHFSFSGDVPTEKWILKPAGPMDSHDLSSCNNEENGLLVDG 1002

Query: 1095 SIPLGNDDEVADEEWLRILSASLL-DGRKSFLETMNMCLGSGQLKLVRTCLTTIAWLSSS 1271
            +I L + +E A EEW R LSA LL +G+KSFLE ++ CLGS   +LVR CLTT+AWLSS+
Sbjct: 1003 TISL-DAEEQAKEEWFRNLSAVLLGNGQKSFLEAISKCLGSDSSELVRVCLTTVAWLSSA 1061

Query: 1272 VASLHVTDFTQSS 1310
            ++SL   +F  S+
Sbjct: 1062 LSSLSDAEFQLSA 1074


>emb|CAN71223.1| hypothetical protein VITISV_011730 [Vitis vinifera]
          Length = 1148

 Score =  451 bits (1159), Expect = e-124
 Identities = 237/433 (54%), Positives = 304/433 (70%), Gaps = 3/433 (0%)
 Frame = +3

Query: 21   NPMENHSWSFHQDRSGNSDYFVERPSFLCIPEDFICPLSGLLFEEPVTLETGQTFETEAI 200
            +P + +  S  +D  GN  YF E   F  IP+DFICPL+G LFE+PVT+ETGQTFE  AI
Sbjct: 643  DPNQTNMESSAKDLHGNCQYFNEGSFFSSIPQDFICPLTGRLFEDPVTIETGQTFERHAI 702

Query: 201  RNWFYKGHRTCPISGVTLEYQAVPLTNFILKRVVDTWKFEHCKTILGFASQLAGNTEEHS 380
            R WF +G+R CP++G  LE   VPLTNFILKRV+D WK E+C+ +L FAS++ G++ EH 
Sbjct: 703  REWFNQGNRNCPVTGKALEGLGVPLTNFILKRVIDGWKSENCRHLLAFASKVEGSSGEHG 762

Query: 381  -NFKDEAAVCIFEHLLTFFSRDERIKKAKLLISLGGLQFLVQRFKCGKLEEKTVIAALLC 557
               KDE A+   E  L+  S++E++  AK LISLGGLQFL +RF+ G LEEKT +AAL+C
Sbjct: 763  VEPKDETAIYALEQFLSGSSKEEKLTNAKHLISLGGLQFLTRRFELGNLEEKTCVAALMC 822

Query: 558  QCIEADSGCRNYIARNIEKICFIELLHCKQAKSIANAVSLLTELICLNRRRDVCSFLRGF 737
             CIEAD  C+N IA+ I+K C +ELLH KQAKS  NAV LLTELIC++R +DV  FL  F
Sbjct: 823  SCIEADYRCKNEIAKYIKKPCLLELLHSKQAKSRTNAVLLLTELICMHRWKDVTLFLSSF 882

Query: 738  ENEEMVNTMHVILIYLQSCPSEQRPXXXXXXXXXXXXXAPKKYSIYREEAIDAITVALES 917
            +NE +++ MHV+L+YLQS   EQRP              P KYSIYREEA+DAI VALE 
Sbjct: 883  QNEGIMSAMHVLLVYLQSSSPEQRPLVAVLLLHLDLLVEPXKYSIYREEAVDAIVVALEG 942

Query: 918  SLTDDKIREKCCHALLVLGGRFSLSGKVMVEDWILKKAGFIDCHELELVDSEE-GIIPLN 1094
            SLTD+ +REKCC ALL+L G FS SG V  E WILK AG +D H+L   ++EE G++   
Sbjct: 943  SLTDENVREKCCRALLILTGHFSFSGDVPTEKWILKPAGPMDSHDLSSCNNEENGLLVDG 1002

Query: 1095 SIPLGNDDEVADEEWLRILSASLL-DGRKSFLETMNMCLGSGQLKLVRTCLTTIAWLSSS 1271
            +I L + +E A EEW R LSA LL +G+KSFLE ++ CLGS   +LVR CLTT+AWLSS+
Sbjct: 1003 TISL-DAEEQAKEEWFRNLSAVLLGNGQKSFLEAISKCLGSDSXELVRVCLTTVAWLSSA 1061

Query: 1272 VASLHVTDFTQSS 1310
            ++SL   +F  S+
Sbjct: 1062 LSSLSDAEFQLSA 1074


>emb|CBI16457.3| unnamed protein product [Vitis vinifera]
          Length = 1084

 Score =  441 bits (1133), Expect = e-121
 Identities = 230/410 (56%), Positives = 294/410 (71%), Gaps = 3/410 (0%)
 Frame = +3

Query: 90   RPSFLCIPEDFICPLSGLLFEEPVTLETGQTFETEAIRNWFYKGHRTCPISGVTLEYQAV 269
            R  F  IP+DFICPL+G LFE+PVT+ETGQTFE  AIR WF +G+R CP++G  LE   V
Sbjct: 602  RSFFSSIPQDFICPLTGRLFEDPVTIETGQTFERHAIREWFNQGNRNCPVTGKALEGLGV 661

Query: 270  PLTNFILKRVVDTWKFEHCKTILGFASQLAGNTEEHS-NFKDEAAVCIFEHLLTFFSRDE 446
            PLTNFILKRV+D WK E+C+ +L FAS++ G++ EH    KDE A+   E  L+  S++E
Sbjct: 662  PLTNFILKRVIDGWKSENCRHLLAFASKVEGSSGEHGVEPKDETAIYALEQFLSGSSKEE 721

Query: 447  RIKKAKLLISLGGLQFLVQRFKCGKLEEKTVIAALLCQCIEADSGCRNYIARNIEKICFI 626
            ++  AK LISLGGLQFL +RF+ G LEEKT +AAL+C CIEAD  C+N IA+ I+K C +
Sbjct: 722  KLTNAKHLISLGGLQFLTRRFELGNLEEKTCVAALMCSCIEADYRCKNEIAKYIKKPCLL 781

Query: 627  ELLHCKQAKSIANAVSLLTELICLNRRRDVCSFLRGFENEEMVNTMHVILIYLQSCPSEQ 806
            ELLH KQAKS  NAV LLTELIC++R +DV  FL  F+NE +++ MHV+L+YLQS   EQ
Sbjct: 782  ELLHSKQAKSRTNAVLLLTELICMHRWKDVTLFLSSFQNEGIMSAMHVLLVYLQSSSPEQ 841

Query: 807  RPXXXXXXXXXXXXXAPKKYSIYREEAIDAITVALESSLTDDKIREKCCHALLVLGGRFS 986
            RP              P+KYSIYREEA+DAI VALE SLTD+ +REKCC ALL+L G FS
Sbjct: 842  RPLVAVLLLHLDLLVEPRKYSIYREEAVDAIVVALEGSLTDENVREKCCRALLILTGHFS 901

Query: 987  LSGKVMVEDWILKKAGFIDCHELELVDSEE-GIIPLNSIPLGNDDEVADEEWLRILSASL 1163
             SG V  E WILK AG +D H+L   ++EE G++   +I L + +E A EEW R LSA L
Sbjct: 902  FSGDVPTEKWILKPAGPMDSHDLSSCNNEENGLLVDGTISL-DAEEQAKEEWFRNLSAVL 960

Query: 1164 L-DGRKSFLETMNMCLGSGQLKLVRTCLTTIAWLSSSVASLHVTDFTQSS 1310
            L +G+KSFLE ++ CLGS   +LVR CLTT+AWLSS+++SL   +F  S+
Sbjct: 961  LGNGQKSFLEAISKCLGSDSSELVRVCLTTVAWLSSALSSLSDAEFQLSA 1010


>ref|XP_002304021.1| predicted protein [Populus trichocarpa] gi|222841453|gb|EEE79000.1|
            predicted protein [Populus trichocarpa]
          Length = 1159

 Score =  415 bits (1066), Expect = e-113
 Identities = 226/428 (52%), Positives = 294/428 (68%), Gaps = 2/428 (0%)
 Frame = +3

Query: 6    DALYSNPMENHSWSFHQDRSGNSDYFVERPSFLCIPEDFICPLSGLLFEEPVTLETGQTF 185
            D L+     N S S  +  +G+ + F E   F  IP+DF+CPL+  LFE+PVTLETGQTF
Sbjct: 654  DLLHPKKAINASASI-EGLNGSHESFDEGSIFESIPQDFVCPLTRQLFEDPVTLETGQTF 712

Query: 186  ETEAIRNWFYKGHRTCPISGVTLEYQAVPLTNFILKRVVDTWKFEHCKTILGFASQLAGN 365
            E EAIR WF +G+RTCP++G TLE   +PLTNFILKR++D+WK E C  +L FASQ+  N
Sbjct: 713  EREAIRKWFNQGNRTCPLTGKTLECPTIPLTNFILKRMIDSWKLERCNHLLSFASQIFKN 772

Query: 366  TEEH-SNFKDEAAVCIFEHLLTFFSRDERIKKAKLLISLGGLQFLVQRFKCGKLEEKTVI 542
            +E + S  ++E A+ I E LL   SR+ER+  AK LISLG L+FL++RF+ G LEEKT++
Sbjct: 773  SEAYDSRQRNEDALFILEKLLASSSREERLTNAKHLISLGVLEFLIKRFEFGSLEEKTLV 832

Query: 543  AALLCQCIEADSGCRNYIARNIEKICFIELLHCKQAKSIANAVSLLTELICLNRRRDVCS 722
            AALL  CIEA+S CRN+IA  I+K C  ELLH  Q+KS  N V LL EL+CL+RR+ V  
Sbjct: 833  AALLSCCIEAESSCRNHIAIKIDKQCLFELLHGNQSKSARNVVGLLIELVCLSRRKGVTQ 892

Query: 723  FLRGFENEEMVNTMHVILIYLQSCPSEQRPXXXXXXXXXXXXXAPKKYSIYREEAIDAIT 902
            F+ G  +E +V+ M ++L+YLQS P+E+ P              P+KYSIYR+EA+DAI+
Sbjct: 893  FISGLPSETIVHAMDILLVYLQSSPAEE-PLVAVLILHLDLLVEPRKYSIYRKEAVDAIS 951

Query: 903  VALESSLTDDKIREKCCHALLVLGGRFSLSGKVMVEDWILKKAGFIDCHELELVDSEEGI 1082
            +ALESSLTD+K+RE+ C AL VLGG FS SG    E WILK+AGF   HE   V+S E  
Sbjct: 952  MALESSLTDEKVREQSCRALNVLGGIFSASGNSSTESWILKQAGFDKNHE---VNSREDN 1008

Query: 1083 IPLNSIPLGNDDEVADEEWLRILSASLL-DGRKSFLETMNMCLGSGQLKLVRTCLTTIAW 1259
            + L+      D+E + EEWLR LS SLL +G+ S LET++ CL SG L LVR CLTTIAW
Sbjct: 1009 LLLDDPLSPEDEEESSEEWLRNLSESLLANGKMSILETISKCLSSGILDLVRACLTTIAW 1068

Query: 1260 LSSSVASL 1283
            LS  ++ L
Sbjct: 1069 LSCGISLL 1076


>gb|ACJ02343.1| U-box domain-containing protein [Helianthus annuus]
          Length = 1050

 Score =  340 bits (872), Expect = 5e-91
 Identities = 174/326 (53%), Positives = 228/326 (69%)
 Frame = +3

Query: 99   FLCIPEDFICPLSGLLFEEPVTLETGQTFETEAIRNWFYKGHRTCPISGVTLEYQAVPLT 278
            FL IPED++CPL+G +FE+PVTLETGQT+E  AI  WF KG++TCP++G TLEYQ VP T
Sbjct: 622  FLNIPEDYMCPLTGHIFEDPVTLETGQTYERVAIMEWFSKGNKTCPVTGKTLEYQTVPFT 681

Query: 279  NFILKRVVDTWKFEHCKTILGFASQLAGNTEEHSNFKDEAAVCIFEHLLTFFSRDERIKK 458
            N ILKR++D+WK +H + +L  AS+  G+  EH  +K EAAV I E LLT F R+E    
Sbjct: 682  NSILKRLIDSWKSKHSREMLSSASRPMGSPREHE-YKAEAAVFILEQLLTVFGREENTAN 740

Query: 459  AKLLISLGGLQFLVQRFKCGKLEEKTVIAALLCQCIEADSGCRNYIARNIEKICFIELLH 638
            AK L++LGGLQFL+QRF+ G L+EKT +AALL  CIEADS CRN++AR ++K   +ELLH
Sbjct: 741  AKHLLALGGLQFLIQRFQYGNLDEKTRVAALLSMCIEADSSCRNHVARYVDKEGVLELLH 800

Query: 639  CKQAKSIANAVSLLTELICLNRRRDVCSFLRGFENEEMVNTMHVILIYLQSCPSEQRPXX 818
            CK+ KS +NAV LL +LICLNRR+DV  FL G + E +            SC  EQ+P  
Sbjct: 801  CKEVKSRSNAVFLLFDLICLNRRKDVRFFLSGLQKEVI------------SCSLEQKPLV 848

Query: 819  XXXXXXXXXXXAPKKYSIYREEAIDAITVALESSLTDDKIREKCCHALLVLGGRFSLSGK 998
                         +KYSIYREEA+D IT AL++SL D+K+RE CC ALL++G R SLSG 
Sbjct: 849  AVLLLHLDLLVDQQKYSIYREEAVDTITSALDTSLFDEKVRETCCRALLIMGARISLSGT 908

Query: 999  VMVEDWILKKAGFIDCHELELVDSEE 1076
            V+ +D+ L+KAGF +  EL+++   E
Sbjct: 909  VITQDYNLQKAGFYNRPELDVIIQPE 934