BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00024326
         (1626 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002522369.1| conserved hypothetical protein [Ricinus comm...   651   0.0  
ref|XP_002310174.1| predicted protein [Populus trichocarpa] gi|2...   644   0.0  
ref|XP_002281443.1| PREDICTED: uncharacterized membrane protein ...   633   e-179
gb|ABR16110.1| unknown [Picea sitchensis]                             405   e-110
ref|XP_002526947.1| conserved hypothetical protein [Ricinus comm...   381   e-103

>ref|XP_002522369.1| conserved hypothetical protein [Ricinus communis]
            gi|223538447|gb|EEF40053.1| conserved hypothetical
            protein [Ricinus communis]
          Length = 550

 Score =  651 bits (1680), Expect = 0.0
 Identities = 312/482 (64%), Positives = 385/482 (79%), Gaps = 3/482 (0%)
 Frame = -1

Query: 1626 RTKKYGPYMKILLICLVPIPIAIWTXXXXXXXXXXXXXXGFVWPVMETFRAISKKDIPLY 1447
            +TKK+GPYMK LLI LVP+PI +WT              GF+WPVMETFRAIS +  P+ 
Sbjct: 68   KTKKFGPYMKCLLILLVPVPIVLWTVIGVAGSAIMGAGYGFIWPVMETFRAISMEG-PIN 126

Query: 1446 IRLHKCFTDGTWTQVWGACTIVRDFSDFSFHTYFSFMDGLLDSKGEE-PIELKVLQLPGC 1270
            ++L +CFT+GTWT VWGACT+VRDF+DFSFH+YFS MDGLL SK EE PIELK+ Q+PGC
Sbjct: 127  MKLIRCFTEGTWTCVWGACTVVRDFADFSFHSYFSVMDGLLKSKDEEEPIELKIAQVPGC 186

Query: 1269 LLVTILGIVVNIPLISFITLYKSPILLLKGWHQLLRDLIGREGPFLETVCVPFAGLWILL 1090
            +L   L I+V++P+I+ I +YK+PILL KGWH+L++DLIGR GPF ETVC+PFAG WILL
Sbjct: 187  ILAACLAILVDVPVITIIVVYKAPILLFKGWHRLIQDLIGRAGPFFETVCIPFAGFWILL 246

Query: 1089 WPVLVLFAVFAGILSSILFGCYSAVVAYQESSTRRGLMYVVAAASVYDEYTNDLLYLREG 910
            WP++VL A  AGI+SS  FGCY+AVVAYQE+ST+RGL+YVVA+ASV+DEYTNDLLYL++G
Sbjct: 247  WPIVVLLATLAGIVSSFGFGCYAAVVAYQENSTKRGLLYVVASASVFDEYTNDLLYLQQG 306

Query: 909  SCYPRPKYRKNADSAASLLPVLGLRQQLESIYADEPLIGSSVN-VQKVTAVVIWDGFFEA 733
            SC+PRPKYRK   S +SLLPV+ L++QL+S Y  EP I    +    +   VIWD FF+A
Sbjct: 307  SCFPRPKYRKQVSSTSSLLPVMRLQEQLDSAYVGEPFIRPPTDKANTLKTAVIWDSFFKA 366

Query: 732  CESIGKELLHIGAIHSDDIDGWKKSKNKIVNIGIPAYAFLECFLSSIKNGCTGFLMRDNV 553
             E IGKELL   AI   D+D W+ SK+ I+N GIPAYAFLECFL SIK+  +GF++RD+V
Sbjct: 367  FEDIGKELLSNEAIGLSDLDAWRNSKSNIINTGIPAYAFLECFLHSIKSSSSGFILRDDV 426

Query: 552  EITSVNRPEGRLFDWLYDPMCVMKEQIKNLNLQETEELYFYKLCLYSGDTRRVESWQNGG 373
            EITSVNRPEGR+FDWLY+PMC+MKEQIK+L+L +TEELYFYK CLY GD  R+ESW N G
Sbjct: 427  EITSVNRPEGRVFDWLYEPMCIMKEQIKSLSLNKTEELYFYKHCLYGGDAARIESWLNDG 486

Query: 372  IAPQDEIRRAQLEGISRRLQGFCLTISRLPTAHRRFDEVVKAIEQ-SRQTEGYGAADDDI 196
            I P D+IRRAQLEG+SRRLQGFCLT+SRLPT+ RRF EVVKAIEQ ++++   G  D D+
Sbjct: 487  IPPDDKIRRAQLEGLSRRLQGFCLTLSRLPTSRRRFFEVVKAIEQDAKESNTLGDEDVDL 546

Query: 195  EA 190
            EA
Sbjct: 547  EA 548


>ref|XP_002310174.1| predicted protein [Populus trichocarpa] gi|222853077|gb|EEE90624.1|
            predicted protein [Populus trichocarpa]
          Length = 548

 Score =  644 bits (1662), Expect = 0.0
 Identities = 305/480 (63%), Positives = 384/480 (80%), Gaps = 1/480 (0%)
 Frame = -1

Query: 1626 RTKKYGPYMKILLICLVPIPIAIWTXXXXXXXXXXXXXXGFVWPVMETFRAISKKDIPLY 1447
            ++K++GPYMK LLI LVP+P+A+WT              GF+WPVMETFRAISK+   + 
Sbjct: 68   KSKRFGPYMKCLLILLVPLPVALWTVVGVVGSAIMGAMYGFIWPVMETFRAISKEG-SIC 126

Query: 1446 IRLHKCFTDGTWTQVWGACTIVRDFSDFSFHTYFSFMDGLLDSKGEEPIELKVLQLPGCL 1267
            ++L +CFTDGTW+ V GACT+VRDF DFSFH+YFS MD LL SKGEEPIELKV Q+PGC+
Sbjct: 127  MKLIRCFTDGTWSCVRGACTVVRDFVDFSFHSYFSVMDELLASKGEEPIELKVAQIPGCI 186

Query: 1266 LVTILGIVVNIPLISFITLYKSPILLLKGWHQLLRDLIGREGPFLETVCVPFAGLWILLW 1087
            L  ILGIVV++ +I+ I L K+P++LLKGWH+L++DLIGREGPFLETVCVPFA LWILLW
Sbjct: 187  LAAILGIVVDVHVITMIVLLKAPLMLLKGWHRLIQDLIGREGPFLETVCVPFASLWILLW 246

Query: 1086 PVLVLFAVFAGILSSILFGCYSAVVAYQESSTRRGLMYVVAAASVYDEYTNDLLYLREGS 907
            P++VL A+ AGI+SS+ FGC++AVVAYQE+ST+RGL+YV+A+AS++DEYTNDLLYL+EGS
Sbjct: 247  PIMVLLAIAAGIISSLGFGCFAAVVAYQENSTKRGLLYVIASASIFDEYTNDLLYLQEGS 306

Query: 906  CYPRPKYRKNADSAASLLPVLGLRQQLESIYADEPLIGSSVN-VQKVTAVVIWDGFFEAC 730
            C+PRP+YRK A S++SLLPV GL ++ E+ Y  E L+ ++ +    + A +IWD FF+  
Sbjct: 307  CFPRPRYRKGATSSSSLLPVKGLHERFEAAYVGEQLVTTTTDKATSLKAAMIWDSFFKTF 366

Query: 729  ESIGKELLHIGAIHSDDIDGWKKSKNKIVNIGIPAYAFLECFLSSIKNGCTGFLMRDNVE 550
            E IGKELL +GAI   D+D W  SK+KI++ GIPAYAFLECFL S K+G  G ++RD VE
Sbjct: 367  EDIGKELLRMGAIGMPDLDAWADSKSKIIDNGIPAYAFLECFLRSFKHGSYGLILRDKVE 426

Query: 549  ITSVNRPEGRLFDWLYDPMCVMKEQIKNLNLQETEELYFYKLCLYSGDTRRVESWQNGGI 370
            IT +NRPEGR+FDWLY+PMC+MKEQI +L L E+EELYFYK CLY GD  R+++W NGG 
Sbjct: 427  ITRLNRPEGRIFDWLYEPMCIMKEQISSLKLDESEELYFYKHCLYGGDENRIKTWLNGGT 486

Query: 369  APQDEIRRAQLEGISRRLQGFCLTISRLPTAHRRFDEVVKAIEQSRQTEGYGAADDDIEA 190
             P DEI+RAQLEGISRRLQGFCLT+SRLPT+ RRF EVVKAIEQ  +     A  DD+EA
Sbjct: 487  PPSDEIKRAQLEGISRRLQGFCLTLSRLPTSRRRFCEVVKAIEQEAKNSSILAGGDDLEA 546


>ref|XP_002281443.1| PREDICTED: uncharacterized membrane protein At3g27390-like [Vitis
            vinifera]
          Length = 547

 Score =  633 bits (1632), Expect = e-179
 Identities = 300/478 (62%), Positives = 379/478 (79%), Gaps = 3/478 (0%)
 Frame = -1

Query: 1626 RTKKYGPYMKILLICLVPIPIAIWTXXXXXXXXXXXXXXGFVWPVMETFRAISKKDIPLY 1447
            RTKK+ PY+K LLI +VPIP+A+WT              GF+ PVM+TF+A+S + + L 
Sbjct: 68   RTKKFDPYLKCLLILMVPIPVALWTVVGVVGSVIMGIGYGFICPVMDTFKAVSMEGVSLR 127

Query: 1446 IRLHKCFTDGTWTQVWGACTIVRDFSDFSFHTYFSFMDGLLDSKGEEPIELKVLQLPGCL 1267
             +L +CFTDGTW+ VWGACTIVRDF+DFSFH+YFS MDGLL+SKGE+PIELKV ++PGC+
Sbjct: 128  TKLIRCFTDGTWSNVWGACTIVRDFADFSFHSYFSVMDGLLESKGEKPIELKVFEIPGCI 187

Query: 1266 LVTILGIVVNIPLISFITLYKSPILLLKGWHQLLRDLIGREGPFLETVCVPFAGLWILLW 1087
            L  ILG++V++P I+ I LYKSPILL KGW+QLL DLIGREGPF ET+CVPFAGL ILLW
Sbjct: 188  LCAILGVLVDVPFITLIVLYKSPILLFKGWNQLLHDLIGREGPFFETICVPFAGLLILLW 247

Query: 1086 PVLVLFAVFAGILSSILFGCYSAVVAYQESSTRRGLMYVVAAASVYDEYTNDLLYLREGS 907
            P++V+ A  AG++SS  FGC +AV+AYQESS +RGL+Y+VA+ S++DE TND LYLREGS
Sbjct: 248  PIVVVLAALAGVISSFFFGCCAAVIAYQESSIKRGLLYIVASMSLFDESTNDFLYLREGS 307

Query: 906  CYPRPKYRKNADSAASLLPVLGLRQQLESIYADEPLI-GSSVNVQKVTAVVIWDGFFEAC 730
            C+PRP+Y K    + +L+ V GL +QL   +A+EPL+  +S   + ++AV+IWD +F+AC
Sbjct: 308  CFPRPRYCKQLTYSPTLVSVNGLNEQL---HANEPLVRTASEKKRALSAVMIWDNYFKAC 364

Query: 729  ESIGKELLHIGAIHSDDIDGWKKSKNKIVNIGIPAYAFLECFLSSIKNGCTGFLMRDNVE 550
            E  GKELL  GA+   D++ W+ SKNKI+NIGIPAY+FLECFL SIK+G  GFLMRDNVE
Sbjct: 365  EHTGKELLRAGAVGMPDLETWRNSKNKIINIGIPAYSFLECFLHSIKSGSPGFLMRDNVE 424

Query: 549  ITSVNRPEGRLFDWLYDPMCVMKEQIKNLNLQETEELYFYKLCLYSGDTRRVESWQNGGI 370
            +TS+NRPEGR+FDW+++PM +MKEQ+K LNL ETEELY YK  LY GD  R+E+WQNGG 
Sbjct: 425  LTSLNRPEGRVFDWMFEPMSIMKEQLKRLNLVETEELYLYKFSLYHGDRERMEAWQNGGF 484

Query: 369  APQDEIRRAQLEGISRRLQGFCLTISRLPTAHRRFDEVVKAIEQS--RQTEGYGAADD 202
             P+DEIRRAQLEGISRRLQGFCLT+SRLPT+ R FD VVK IEQ   R  +G+ A DD
Sbjct: 485  PPEDEIRRAQLEGISRRLQGFCLTLSRLPTSRRWFDGVVKEIEQEARRGLDGFDALDD 542


>gb|ABR16110.1| unknown [Picea sitchensis]
          Length = 605

 Score =  405 bits (1042), Expect = e-110
 Identities = 212/474 (44%), Positives = 303/474 (63%), Gaps = 15/474 (3%)
 Frame = -1

Query: 1626 RTKKYGPYMKILLICLVPIPIAIWTXXXXXXXXXXXXXXGFVWPVMETFRAISKKDIPLY 1447
            R K+ GP +K +L   +P+P+ +W               G   P++ TF AI ++    +
Sbjct: 68   RAKRLGPNLKAVLCIGLPVPLILWPLVGLTGSILVGLGYGLFTPLVATFEAIGEEHENKF 127

Query: 1446 IRLHKCFTDGTWTQVWGACTIVRDFSDFSFHTYFSFMDGLLDS--KGEEPIELKVLQLPG 1273
            +    C   GTW+ V G+ T+VRD +D  FH+YFS MD L +   K   P+++K+L+LPG
Sbjct: 128  V---DCILKGTWSTVKGSFTVVRDVTDTCFHSYFSLMDDLREQPPKNGSPLDIKLLELPG 184

Query: 1272 CLLVTILGIVVNIPLISFITLYKSPILLLKGWHQLLRDLIGREGPFLETVCVPFAGLWIL 1093
             LLV +LG++V++P+I  I L+KSP +LLKGWH+L+ DLIGREGPF+ETVCVPFAGL IL
Sbjct: 185  SLLVGLLGVIVDVPMIVLIALWKSPYMLLKGWHRLVHDLIGREGPFMETVCVPFAGLAIL 244

Query: 1092 LWPVLVLFAVFAGILSSILFGCYSAVVAYQESSTRRGLMYVVAAASVYDEYTNDLLYLRE 913
            LWP++V+ AV +  +SS   G Y+AVV YQESS + GL YVV+A +++DEY ND LY+RE
Sbjct: 245  LWPLVVIAAVLSAFVSSFFLGFYAAVVVYQESSIKLGLAYVVSALALFDEYANDFLYMRE 304

Query: 912  GSCYPRPKYR-KNADSAASL-----LPVLGLRQQLESIYADEPLIGS------SVNVQKV 769
            GSC+PRPKYR +N   + +L     L   G   +  SI     + G         ++Q++
Sbjct: 305  GSCFPRPKYRSQNMPLSGTLSRRYSLSKEGSNGKSGSISPGNEMPGPQRSKSLKKSIQEM 364

Query: 768  TAVVIWDGFFEACESIGKELLHIGAIHSDDIDGWK-KSKNKIVNIGIPAYAFLECFLSSI 592
              + +WD  FE+ E  G+ L+    I   DI+ W   +K KI+++G+PAY+ L+  L S 
Sbjct: 365  KTMQMWDHLFESYERYGRNLVGDNIIKLSDIEEWNHTAKCKILSVGLPAYSILQNLLCSA 424

Query: 591  KNGCTGFLMRDNVEITSVNRPEGRLFDWLYDPMCVMKEQIKNLNLQETEELYFYKLCLYS 412
            K+   GF   D  EIT++NR   RLF+W ++P+ ++KEQIK   L+E+EELY  KL L S
Sbjct: 425  KHDSAGFRFTDGTEITAMNRTGDRLFEWFFEPLMIIKEQIKAEQLEESEELYLSKLVLLS 484

Query: 411  GDTRRVESWQNGGIAPQDEIRRAQLEGISRRLQGFCLTISRLPTAHRRFDEVVK 250
            G   R+++WQNGGI P D++RRA+LE +SRRLQG   +IS +PT  RRF  VV+
Sbjct: 485  GFPDRMKAWQNGGIPPPDDVRRAELEALSRRLQGIAASISIMPTFRRRFQNVVR 538


>ref|XP_002526947.1| conserved hypothetical protein [Ricinus communis]
            gi|223533699|gb|EEF35434.1| conserved hypothetical
            protein [Ricinus communis]
          Length = 582

 Score =  381 bits (979), Expect = e-103
 Identities = 204/467 (43%), Positives = 287/467 (61%), Gaps = 4/467 (0%)
 Frame = -1

Query: 1626 RTKKYGPYMKILLICLVPIPIAIWTXXXXXXXXXXXXXXGFVWPVMETFRAISKKDIPLY 1447
            R K+ GP +KI+L  L+P P+ +W               GF+ PV  TF A+ +    + 
Sbjct: 68   RAKQLGPVLKIVLCILLPAPLILWLAVGIVGSIIGGILYGFLSPVFATFDAVGEGKSNV- 126

Query: 1446 IRLHKCFTDGTWTQVWGACTIVRDFSDFSFHTYFSFMDGLLDSKGEEP--IELKVLQLPG 1273
              L+ CF DGTW  +  +CTIVRDF+DF +H+YFS+MD L   +  +    E+++L LPG
Sbjct: 127  --LYHCFYDGTWDTIQKSCTIVRDFADFCYHSYFSYMDDLQQQQPPDRKYYEIRLLYLPG 184

Query: 1272 CLLVTILGIVVNIPLISFITLYKSPILLLKGWHQLLRDLIGREGPFLETVCVPFAGLWIL 1093
             L+  +LG +++ PLISFI + KSP +L KGWH+L  DLIGREGPFLET+CVPFAGL IL
Sbjct: 185  ALIAGLLGFILDFPLISFIAICKSPYMLFKGWHRLFHDLIGREGPFLETICVPFAGLAIL 244

Query: 1092 LWPVLVLFAVFAGILSSILFGCYSAVVAYQESSTRRGLMYVVAAASVYDEYTNDLLYLRE 913
            LWP+ V+ AV   ++SSI  G Y+ V+ YQESS   GL Y+VA+ ++YDEY+ND L + E
Sbjct: 245  LWPLAVVGAVLGSMISSIFLGAYAGVIVYQESSFWYGLCYIVASWALYDEYSNDTLDMPE 304

Query: 912  GSCYPRPKYRKNADSAASLLPVLGLRQQLESIYADEPLIGSSVNVQKVTAVVIWDGFFEA 733
            GSC+PRPKYRKNA+ + S        +     Y  +PL      +  +    + +G F+ 
Sbjct: 305  GSCFPRPKYRKNAELSKSTSRASSFSRPSSFKYRLDPL----PPMVDLKPFELLNGLFKE 360

Query: 732  CESIGKELLHIGAIHSDDIDGWKKSK-NKIVNIGIPAYAFLECFLSSIKNGCTGFLMRDN 556
            C+  G+  L  G I + DI+  K +K   +VNIG+PAY  L+  L S K    G L+ DN
Sbjct: 361  CQKHGENFLSEGLITAQDIEDAKSNKGGTVVNIGLPAYCLLQTLLRSAKANSEGILLSDN 420

Query: 555  -VEITSVNRPEGRLFDWLYDPMCVMKEQIKNLNLQETEELYFYKLCLYSGDTRRVESWQN 379
              EITS NRP+   FDW ++P  VMK+QIK  NL E EE Y  KL L + D  +++S  +
Sbjct: 421  HTEITSYNRPKDAFFDWFFNPFLVMKDQIKAQNLSEEEEKYLCKLVLLNDDFIKLKS-AS 479

Query: 378  GGIAPQDEIRRAQLEGISRRLQGFCLTISRLPTAHRRFDEVVKAIEQ 238
             G AP  E +RA+L+ ++RRL+G   ++SR PT+ R F  +VK + +
Sbjct: 480  IGPAPDSERKRAELDALARRLRGLTKSVSRYPTSRRHFQNLVKTLSE 526