BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00022921
         (1423 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003589174.1| Trimethylguanosine synthase [Medicago trunca...   362   1e-97
emb|CBI19510.3| unnamed protein product [Vitis vinifera]              355   1e-95
ref|XP_002282801.1| PREDICTED: uncharacterized protein LOC100262...   355   1e-95
dbj|BAJ90926.1| predicted protein [Hordeum vulgare subsp. vulgare]    354   4e-95
ref|NP_973978.2| S-adenosylmethionine-dependent methyltransferas...   352   1e-94

>ref|XP_003589174.1| Trimethylguanosine synthase [Medicago truncatula]
            gi|355478222|gb|AES59425.1| Trimethylguanosine synthase
            [Medicago truncatula]
          Length = 701

 Score =  362 bits (929), Expect = 1e-97
 Identities = 196/416 (47%), Positives = 249/416 (59%), Gaps = 37/416 (8%)
 Frame = +2

Query: 83   FGDWKTYWDSYYCRCYFFNIRTQECTWDPPAGMEHLIYLDMSIESKVIGXXXXXXXXXXX 262
            FGDW   WD++Y R YF+N++T   TWDPP GMEHL +          G           
Sbjct: 293  FGDWMVLWDTFYGRRYFYNVKTDTSTWDPPPGMEHLAF----------GGCTELDDSETL 342

Query: 263  XSSQNSHTLDSVDS------QYNLNSMKHD-------------------TSQGQKLNE-T 364
             SS+   T  S+          NL+  +H+                    S+ Q L+  +
Sbjct: 343  KSSEECETQSSIKQPEETLVDENLSGNQHEEYSAEIGVAAGNLVSDIATNSEDQFLHHPS 402

Query: 365  VEGHELTA--GNFDNSLITSTVRRIAEPLNE----SHEVKQSSVQSSIADQMEVTVDNXX 526
             E  E T+  G      +++T+  +    N+    + EV  +  +  + D +E+   +  
Sbjct: 403  DENLERTSCNGGVSRCSVSNTLDHVVSSNNKCSQATSEVDHTPTEYMVIDTLELDSKSDP 462

Query: 527  XXXXXXXXXXXSISADK-----EDLLYQEFGEELSPIINKYWWQRYQLFSKFDCGILMDE 691
                            K     EDL +Q+  E  S  I KYW QRY LFS+FD G+ MDE
Sbjct: 463  FMSKQEKKVKRKHRRKKLYYETEDLEFQKMPEAYSATIEKYWCQRYILFSRFDDGVKMDE 522

Query: 692  EGWFSVTPELIAKHHASRCGSGTIVDCFTGVGGNAIQFALRCNHVIAIDIDPKKIDYAHH 871
            EGWFSVTPE+IA H ASRC  GT++DCFTG GGNAIQFA RC HV+AIDIDP KIDYA H
Sbjct: 523  EGWFSVTPEIIAHHQASRCAGGTLIDCFTGAGGNAIQFAQRCRHVVAIDIDPLKIDYARH 582

Query: 872  NAAIYGVDERVDFIRGDSFLLASNLKADTVFLSPPWGGPAYSKVKAFDIKTMLKPYDGYF 1051
            NAAIY VD+++DFI GD F+LA  LKADTVFLSPPWGGP YSKV  +D+KTML+P+DG+ 
Sbjct: 583  NAAIYRVDDQIDFIAGDFFVLAPKLKADTVFLSPPWGGPDYSKVVTYDMKTMLRPHDGHT 642

Query: 1052 LFTVAKKISPRIVMFLPRNVNLNQLAELSLSVSPPWSLEVEQNFLNGRLKAITAYF 1219
            LF VA++I+ R+ MFLPRNVN NQLAELSLS SPPWS+EVE+  LNG+LKA+TAYF
Sbjct: 643  LFNVAQEIASRVAMFLPRNVNFNQLAELSLSASPPWSIEVEKVHLNGKLKAVTAYF 698


>emb|CBI19510.3| unnamed protein product [Vitis vinifera]
          Length = 683

 Score =  355 bits (912), Expect = 1e-95
 Identities = 167/229 (72%), Positives = 194/229 (84%)
 Frame = +2

Query: 563  ISADKEDLLYQEFGEELSPIINKYWWQRYQLFSKFDCGILMDEEGWFSVTPELIAKHHAS 742
            +S + E   +Q   E+ S  I+KYWWQRY LFSK+D GI MD+EGWFSVTPE+IA+HHAS
Sbjct: 452  LSNENEGFEFQGLLEDSSADISKYWWQRYLLFSKYDDGIKMDKEGWFSVTPEIIARHHAS 511

Query: 743  RCGSGTIVDCFTGVGGNAIQFALRCNHVIAIDIDPKKIDYAHHNAAIYGVDERVDFIRGD 922
            RCGSG IVDCFTGVGGNAIQFA R  HVIAIDIDPKKI+YA HNAAIYGVD+R+DFI+GD
Sbjct: 512  RCGSGIIVDCFTGVGGNAIQFAQRSKHVIAIDIDPKKIEYAQHNAAIYGVDDRIDFIKGD 571

Query: 923  SFLLASNLKADTVFLSPPWGGPAYSKVKAFDIKTMLKPYDGYFLFTVAKKISPRIVMFLP 1102
            SFLLAS LKADTVFLSPPWGGP Y+KV+ +DIKTMLKP+DG+FLF   KK++ R+VMFLP
Sbjct: 572  SFLLASTLKADTVFLSPPWGGPDYAKVETYDIKTMLKPHDGFFLFNTVKKVASRVVMFLP 631

Query: 1103 RNVNLNQLAELSLSVSPPWSLEVEQNFLNGRLKAITAYFCDPSVHDTSS 1249
            RNV++NQLAELSLS  PPW LEVE+NFLNG+LKA+TAYF D S+    S
Sbjct: 632  RNVDVNQLAELSLSADPPWFLEVEKNFLNGKLKAVTAYFSDTSIQKNIS 680



 Score = 60.8 bits (146), Expect = 8e-07
 Identities = 23/37 (62%), Positives = 28/37 (75%)
 Frame = +2

Query: 83  FGDWKTYWDSYYCRCYFFNIRTQECTWDPPAGMEHLI 193
           FGDW+ YWDS+Y R YF+NI+T E TW  P GME L+
Sbjct: 215 FGDWRVYWDSFYMRNYFYNIKTHESTWFQPPGMEFLL 251


>ref|XP_002282801.1| PREDICTED: uncharacterized protein LOC100262697 [Vitis vinifera]
          Length = 780

 Score =  355 bits (912), Expect = 1e-95
 Identities = 167/229 (72%), Positives = 194/229 (84%)
 Frame = +2

Query: 563  ISADKEDLLYQEFGEELSPIINKYWWQRYQLFSKFDCGILMDEEGWFSVTPELIAKHHAS 742
            +S + E   +Q   E+ S  I+KYWWQRY LFSK+D GI MD+EGWFSVTPE+IA+HHAS
Sbjct: 549  LSNENEGFEFQGLLEDSSADISKYWWQRYLLFSKYDDGIKMDKEGWFSVTPEIIARHHAS 608

Query: 743  RCGSGTIVDCFTGVGGNAIQFALRCNHVIAIDIDPKKIDYAHHNAAIYGVDERVDFIRGD 922
            RCGSG IVDCFTGVGGNAIQFA R  HVIAIDIDPKKI+YA HNAAIYGVD+R+DFI+GD
Sbjct: 609  RCGSGIIVDCFTGVGGNAIQFAQRSKHVIAIDIDPKKIEYAQHNAAIYGVDDRIDFIKGD 668

Query: 923  SFLLASNLKADTVFLSPPWGGPAYSKVKAFDIKTMLKPYDGYFLFTVAKKISPRIVMFLP 1102
            SFLLAS LKADTVFLSPPWGGP Y+KV+ +DIKTMLKP+DG+FLF   KK++ R+VMFLP
Sbjct: 669  SFLLASTLKADTVFLSPPWGGPDYAKVETYDIKTMLKPHDGFFLFNTVKKVASRVVMFLP 728

Query: 1103 RNVNLNQLAELSLSVSPPWSLEVEQNFLNGRLKAITAYFCDPSVHDTSS 1249
            RNV++NQLAELSLS  PPW LEVE+NFLNG+LKA+TAYF D S+    S
Sbjct: 729  RNVDVNQLAELSLSADPPWFLEVEKNFLNGKLKAVTAYFSDTSIQKNIS 777



 Score = 60.8 bits (146), Expect = 8e-07
 Identities = 23/37 (62%), Positives = 28/37 (75%)
 Frame = +2

Query: 83  FGDWKTYWDSYYCRCYFFNIRTQECTWDPPAGMEHLI 193
           FGDW+ YWDS+Y R YF+NI+T E TW  P GME L+
Sbjct: 312 FGDWRVYWDSFYMRNYFYNIKTHESTWFQPPGMEFLL 348


>dbj|BAJ90926.1| predicted protein [Hordeum vulgare subsp. vulgare]
          Length = 690

 Score =  354 bits (908), Expect = 4e-95
 Identities = 181/382 (47%), Positives = 235/382 (61%), Gaps = 1/382 (0%)
 Frame = +2

Query: 77   YDFGDWKTYWDSYYCRCYFFNIRTQECTWDPPAGMEHLIYLDMSIESKVIGXXXXXXXXX 256
            Y+ GDW+  WD +Y R Y++NI+TQE TWDPP G+E  +    +  S+ I          
Sbjct: 316  YECGDWQVLWDQFYSRYYYYNIQTQESTWDPPQGLEDFVSYCSTYSSQGIDLQYGANASP 375

Query: 257  XXXSSQNSHTLDSVDSQYNLNSMKHDT-SQGQKLNETVEGHELTAGNFDNSLITSTVRRI 433
                     T D      +L+  +HD  ++ Q L++  E           S+  S +  I
Sbjct: 376  R---DNGETTFDQAGDGSHLDEQRHDLYNEAQSLSDDKE-----------SIYPSVIATI 421

Query: 434  AEPLNESHEVKQSSVQSSIADQMEVTVDNXXXXXXXXXXXXXSISADKEDLLYQEFGEEL 613
             E  +  +    SSV  ++    E T                 +   +     Q+  E +
Sbjct: 422  DEVQDGENRQNDSSVAEALEVNQEATTTKKKKR----------VRRSQSSHSCQDLAENI 471

Query: 614  SPIINKYWWQRYQLFSKFDCGILMDEEGWFSVTPELIAKHHASRCGSGTIVDCFTGVGGN 793
            S  I KYW QRY LFS FD GI MDEEGWFSVTPE IAKHHASR G+G ++DCFTGVGGN
Sbjct: 472  SNDIAKYWNQRYSLFSLFDSGIKMDEEGWFSVTPEPIAKHHASRVGAGILIDCFTGVGGN 531

Query: 794  AIQFALRCNHVIAIDIDPKKIDYAHHNAAIYGVDERVDFIRGDSFLLASNLKADTVFLSP 973
             IQFA +CNHV A+DIDP+KID A HNA +YGV++ +DFI GD   +A +LK +TVF+SP
Sbjct: 532  TIQFAAKCNHVFAVDIDPQKIDCAQHNATVYGVNDHIDFIIGDFIRIAPHLKGETVFMSP 591

Query: 974  PWGGPAYSKVKAFDIKTMLKPYDGYFLFTVAKKISPRIVMFLPRNVNLNQLAELSLSVSP 1153
            PWGGP Y+KV  +DIKTMLKP DGY LF VA  I+ R+VMFLPRN +L+QLA++ LSV P
Sbjct: 592  PWGGPDYAKVDVYDIKTMLKPCDGYHLFKVATAIASRVVMFLPRNSDLDQLADMCLSVDP 651

Query: 1154 PWSLEVEQNFLNGRLKAITAYF 1219
            PW++EVE+N+LNG+LKAITAYF
Sbjct: 652  PWAVEVEKNYLNGKLKAITAYF 673


>ref|NP_973978.2| S-adenosylmethionine-dependent methyltransferase domain-containing
            protein [Arabidopsis thaliana]
            gi|332193981|gb|AEE32102.1|
            S-adenosylmethionine-dependent methyltransferase
            domain-containing protein [Arabidopsis thaliana]
          Length = 538

 Score =  352 bits (904), Expect = 1e-94
 Identities = 194/406 (47%), Positives = 245/406 (60%), Gaps = 16/406 (3%)
 Frame = +2

Query: 50   SNTVDIKCIYDFGDWKTYWDSYYCRCYFFNIRTQECTWDPPAGMEHLIYLDMS------- 208
            S T+D    +D   WK YWDS+Y R YF+N +TQE  W+PP GMEHL Y D S       
Sbjct: 158  SETIDS---HDSSVWKVYWDSFYGRSYFYNFKTQESKWEPPLGMEHLAYSDESHNLSELV 214

Query: 209  -------IESKVIGXXXXXXXXXXXXS-SQNSHTLDSVDSQYNLNSMKHDTSQG-QKLNE 361
                   +   V+G               Q+    ++ +   +L     +TS G Q L+ 
Sbjct: 215  IEKHHDDLSGTVLGDDVPFDKADDLGGVCQSQLEAEATEEVNSLIDTYQETSIGNQSLDI 274

Query: 362  TVEGHELTAGNFDNSLITSTVRRIAEPLNESHEVKQSSVQSSIADQMEVTVDNXXXXXXX 541
            T  G E T      + + S+VR+  +    S   K+  + S    +M+            
Sbjct: 275  TSLGEEGTG-----AYVVSSVRKAKKESRRSR-AKKKLLNSYTGTEMKGVP--------- 319

Query: 542  XXXXXXSISADKEDLLYQEFGEELSPIINKYWWQRYQLFSKFDCGILMDEEGWFSVTPEL 721
                                 EE SPI+ KYW QRY LFS+FD GI MDEEGWFSVTPEL
Sbjct: 320  ---------------------EEYSPILGKYWCQRYLLFSRFDEGIKMDEEGWFSVTPEL 358

Query: 722  IAKHHASRCGSGTIVDCFTGVGGNAIQFALRCNHVIAIDIDPKKIDYAHHNAAIYGVDER 901
            IAKHHA+RC  G ++DCFTGVGGNAIQFA R ++VIAID+DPKK+D A HNAAIYGV ++
Sbjct: 359  IAKHHATRCNEGIVIDCFTGVGGNAIQFASRSHYVIAIDLDPKKLDLAKHNAAIYGVADK 418

Query: 902  VDFIRGDSFLLASNLKADTVFLSPPWGGPAYSKVKAFDIKTMLKPYDGYFLFTVAKKISP 1081
            +DF++GD F LA NLKA TVFLSPPWGGP Y K   +D+KTML+P DG  LF  A  I+ 
Sbjct: 419  IDFVKGDFFDLAHNLKAGTVFLSPPWGGPDYLKASTYDMKTMLRPRDGDALFKAAMNIAS 478

Query: 1082 RIVMFLPRNVNLNQLAELSLSVSPPWSLEVEQNFLNGRLKAITAYF 1219
             I+MFLPRNV++NQLAEL+LS SPPWSLEVE+N+LNG+LKA+TAY+
Sbjct: 479  TIIMFLPRNVDINQLAELALSTSPPWSLEVEKNYLNGKLKAVTAYY 524