BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00022920
         (1826 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFK79026.1| cytochrome P450 CYP82T1 [Bupleurum chinense]          1018   0.0  
ref|XP_002323130.1| cytochrome P450 [Populus trichocarpa] gi|222...   538   e-150
ref|XP_003635525.1| PREDICTED: cytochrome P450 82C4-like [Vitis ...   532   e-148
ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinif...   525   e-146
ref|XP_002274740.1| PREDICTED: cytochrome P450 82C4 [Vitis vinif...   523   e-146

>gb|AFK79026.1| cytochrome P450 CYP82T1 [Bupleurum chinense]
          Length = 526

 Score = 1018 bits (2632), Expect = 0.0
 Identities = 502/526 (95%), Positives = 514/526 (97%)
 Frame = -3

Query: 1797 MDAVGLLQLIAGFVALVLFYTLWSSCTRNHDEKKKTQAPKPNGAWPIIGHIPLLASTKPA 1618
            MDA+GLLQLIAGFVALV FYTLWSS T+NHDEKKKTQAPKPNGAWPIIGHIPLLASTKPA
Sbjct: 1    MDALGLLQLIAGFVALVSFYTLWSSRTKNHDEKKKTQAPKPNGAWPIIGHIPLLASTKPA 60

Query: 1617 WKILADMADKCGPVFRIQLGWQNAVVVSSKEAVMQIFTTNDNNFMTRPTSLALKYMGYNG 1438
             KIL DMADK GPVFRIQLGWQNAVVVSSKEAVMQIFTTNDNNFMTRPTSL LKYMGYNG
Sbjct: 61   CKILGDMADKYGPVFRIQLGWQNAVVVSSKEAVMQIFTTNDNNFMTRPTSLTLKYMGYNG 120

Query: 1437 AFFAFSPYSTLYREMRKVSIFEVLSNSRMELLKPLRASEMTTCIKELYSLCCKNGIEVPV 1258
            AFFAF+PYSTLYREMRK SIFEVLSNSRMELLKPLRASEMTTCIKELYSLCCKNGI VPV
Sbjct: 121  AFFAFAPYSTLYREMRKASIFEVLSNSRMELLKPLRASEMTTCIKELYSLCCKNGIVVPV 180

Query: 1257 KLNIEKWVQQVIINLVIQMLARKRYSSIGEGETEVESRRFKKAFEEFFILAGAFDLSDVI 1078
            KLNIEKWVQQVIINL++QMLARKRYSSIGEGET+VESRRFKKAFEEFFILAGAF+LSDVI
Sbjct: 181  KLNIEKWVQQVIINLMLQMLARKRYSSIGEGETQVESRRFKKAFEEFFILAGAFELSDVI 240

Query: 1077 PFIEWMDLQGNRRAMIRTAKELDVFMSSWIDEHKKSREQDQLKEDRDFIDVMISLFPEPD 898
            PFIEWMDLQGNRRAMIRTAKELDVFMSSWIDEHKKSREQDQLKEDRDFIDVMISLFPEP 
Sbjct: 241  PFIEWMDLQGNRRAMIRTAKELDVFMSSWIDEHKKSREQDQLKEDRDFIDVMISLFPEPH 300

Query: 897  AFVHGYKSTEVIKATVMSVIMGGSDAPAITLTWALSLLLNNKNVLEKAQQELDDHIGKER 718
            A VHGYKST+VIKATVMSVIMGGSDAPAITLTWALSLLLNN NVLEKAQQELDDHIGK+R
Sbjct: 301  ASVHGYKSTDVIKATVMSVIMGGSDAPAITLTWALSLLLNNNNVLEKAQQELDDHIGKDR 360

Query: 717  WVEESDIRHLVYLQAILKETLRLYPGGPLGVPRKAKEDCTVAGYHVPKGTQLWVNIWKLH 538
            WVEESDIRHLVYLQAILKETLRLYPGGPLG+PRKAKEDCTVAGYHVPKGTQLWVNIWKLH
Sbjct: 361  WVEESDIRHLVYLQAILKETLRLYPGGPLGIPRKAKEDCTVAGYHVPKGTQLWVNIWKLH 420

Query: 537  RDSESWTDPYEFQPERFLTSHAGVDVRGQQFEYIPYSSGRRSCPGITASMQMMQLTLARL 358
            RDSE+WT PYEFQPERFLTSHAGVDVRGQQFEYIPYSSGRRSCPGITASMQMMQLTLARL
Sbjct: 421  RDSETWTAPYEFQPERFLTSHAGVDVRGQQFEYIPYSSGRRSCPGITASMQMMQLTLARL 480

Query: 357  LQGFNLVSPTNEPVDMTEAAGISLHRKYPFEVVLTPRLPCKLYQMN 220
            LQGFNLVSPTNEPVDMTEAAGIS+HRKYPFEVVLTPRLPCKLYQMN
Sbjct: 481  LQGFNLVSPTNEPVDMTEAAGISMHRKYPFEVVLTPRLPCKLYQMN 526


>ref|XP_002323130.1| cytochrome P450 [Populus trichocarpa] gi|222867760|gb|EEF04891.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 499

 Score =  538 bits (1386), Expect = e-150
 Identities = 265/504 (52%), Positives = 360/504 (71%), Gaps = 1/504 (0%)
 Frame = -3

Query: 1734 LWSSCTRNHDEKKKTQAPKPNGAWPIIGHIPLLASTKPAWKILADMADKCGPVFRIQLGW 1555
            +W   + +H   K    P+P+GAWP+IGH+ +L S  P ++IL D+A K GPVF I+LG 
Sbjct: 3    IWRILSTSHKRNKTLPPPEPSGAWPLIGHLRILNSQIPFFRILGDLAVKHGPVFSIRLGM 62

Query: 1554 QNAVVVSSKEAVMQIFTTNDNNFMTRPTSLALKYMGYNGAFFAFSPYSTLYREMRKVSIF 1375
            +  +V+SS E+V + F TND  F+ RP+  A KYMGY+ AFF F PY   + EMRK++  
Sbjct: 63   RRTLVISSWESVKECFKTNDRKFLNRPSFAASKYMGYDDAFFGFHPYGEYWLEMRKIATQ 122

Query: 1374 EVLSNSRMELLKPLRASEMTTCIKELYSLCCKNGIEVPVKLNIEKWVQQVIINLVIQMLA 1195
            E+LSN R+ELLK +R SE+ TCIKEL++ C    + V    ++ +W   V+ N++ +++A
Sbjct: 123  ELLSNRRLELLKHVRVSEIETCIKELHTTCSNGSVLV----DMSQWFSCVVANVMFRLIA 178

Query: 1194 RKRYSSIGEGETEVESRRFKKAFEEFFILAGAFDLSDVIPFIEWMDLQGNRRAMIRTAKE 1015
             KRY S G G+   +S  F +   EFF L G   +SD+IPF EWMDLQG+ ++M R AKE
Sbjct: 179  GKRYCS-GIGK---DSGAFGRLVREFFYLGGVLVISDLIPFTEWMDLQGHVKSMKRVAKE 234

Query: 1014 LDVFMSSWIDEHKKSREQDQL-KEDRDFIDVMISLFPEPDAFVHGYKSTEVIKATVMSVI 838
            LD  +S W+ EH + RE+ ++ KE++DF+DVM+      D  + GYK   ++KAT +++I
Sbjct: 235  LDHVVSGWLVEHLQRREEGRVRKEEKDFMDVMLESLAVGDDPIFGYKRETIVKATALNLI 294

Query: 837  MGGSDAPAITLTWALSLLLNNKNVLEKAQQELDDHIGKERWVEESDIRHLVYLQAILKET 658
            + G+D  ++TLTWALSLLLN+  VL++AQ+E+D H+G  RWVEESDI++LVYLQAI+KET
Sbjct: 295  LAGTDTTSVTLTWALSLLLNHTEVLKRAQKEIDVHVGTTRWVEESDIKNLVYLQAIVKET 354

Query: 657  LRLYPGGPLGVPRKAKEDCTVAGYHVPKGTQLWVNIWKLHRDSESWTDPYEFQPERFLTS 478
            LRLYP GPL VPR++ EDC V GY VP+GTQL VN WKLHRD+  W +PYEF PERFLTS
Sbjct: 355  LRLYPPGPLLVPRESLEDCYVDGYLVPRGTQLLVNAWKLHRDARIWENPYEFHPERFLTS 414

Query: 477  HAGVDVRGQQFEYIPYSSGRRSCPGITASMQMMQLTLARLLQGFNLVSPTNEPVDMTEAA 298
            H   DVRGQQFEY+P+ SGRR CPGI++S+QM+ LTL+RLLQGFN  +P N  VDM+E  
Sbjct: 415  HGSTDVRGQQFEYVPFGSGRRLCPGISSSLQMLHLTLSRLLQGFNFSTPMNAQVDMSEGL 474

Query: 297  GISLHRKYPFEVVLTPRLPCKLYQ 226
            G++L +  P EVVLTPRL  ++YQ
Sbjct: 475  GLTLPKATPLEVVLTPRLENEIYQ 498


>ref|XP_003635525.1| PREDICTED: cytochrome P450 82C4-like [Vitis vinifera]
          Length = 530

 Score =  532 bits (1370), Expect = e-148
 Identities = 266/531 (50%), Positives = 373/531 (70%)
 Frame = -3

Query: 1821 THTDTLLSMDAVGLLQLIAGFVALVLFYTLWSSCTRNHDEKKKTQAPKPNGAWPIIGHIP 1642
            + T+    M+ +  L  IAGF+ L+L Y +W   +  H  K ++ AP+P+GAWP +GH+ 
Sbjct: 5    SQTEAQFIMNLLSHLLAIAGFMGLLLLYNVWRVRSFAHGGKGRS-APEPSGAWPFVGHLH 63

Query: 1641 LLASTKPAWKILADMADKCGPVFRIQLGWQNAVVVSSKEAVMQIFTTNDNNFMTRPTSLA 1462
            LL+   P ++ LA MADK GPVF I+LG   A+VVSS EAV +  TTND  F +RP+S A
Sbjct: 64   LLSGPTPIFRTLAAMADKHGPVFMIRLGMHRALVVSSHEAVKECLTTNDKAFASRPSSSA 123

Query: 1461 LKYMGYNGAFFAFSPYSTLYREMRKVSIFEVLSNSRMELLKPLRASEMTTCIKELYSLCC 1282
             K +GYN A F F+PY  L+REMRK+S+ E+LSN  +  LK +  SE+  CI++LYSL  
Sbjct: 124  GKLLGYNYAGFGFAPYGPLWREMRKLSVTELLSNRPLNELKHVLVSELDVCIRDLYSLGK 183

Query: 1281 KNGIEVPVKLNIEKWVQQVIINLVIQMLARKRYSSIGEGETEVESRRFKKAFEEFFILAG 1102
            +     P+K+ + +W++Q+  N+V++M+A KRY   G    E E+R      ++F  L G
Sbjct: 184  ETNWVNPIKVAMSEWLEQLTFNVVLRMVAGKRYFGNGVHGNE-EARHAIAVIKKFIFLTG 242

Query: 1101 AFDLSDVIPFIEWMDLQGNRRAMIRTAKELDVFMSSWIDEHKKSREQDQLKEDRDFIDVM 922
             F  SD IPF+EWMDLQG+  +M R A++LD F+  W++EH    + D     +DFIDVM
Sbjct: 243  VFVASDAIPFVEWMDLQGHLGSMKRVAEQLDPFVEGWVEEHVTKLKSDPSSR-QDFIDVM 301

Query: 921  ISLFPEPDAFVHGYKSTEVIKATVMSVIMGGSDAPAITLTWALSLLLNNKNVLEKAQQEL 742
            +S+  +   F  G++   VIKATVM++I+GGS+  +I   W LS LLNN++ L++AQ+EL
Sbjct: 302  LSVLKDNSMF--GHRRETVIKATVMTLIVGGSETTSIASIWILSALLNNRHTLKRAQEEL 359

Query: 741  DDHIGKERWVEESDIRHLVYLQAILKETLRLYPGGPLGVPRKAKEDCTVAGYHVPKGTQL 562
            D  +G+ RWVEESDI++L+YLQA +KETLRLYP GPL VP +A +DC+V GYH+PKGT+L
Sbjct: 360  DLKVGRGRWVEESDIQNLIYLQAAVKETLRLYPPGPLLVPHEAIQDCSVCGYHIPKGTRL 419

Query: 561  WVNIWKLHRDSESWTDPYEFQPERFLTSHAGVDVRGQQFEYIPYSSGRRSCPGITASMQM 382
             VN+WKLHRD ++W+DP EFQPERFLT+HA ++V GQ  E IP+SSGRRSCPGI  ++Q+
Sbjct: 420  LVNVWKLHRDPDAWSDPEEFQPERFLTTHANLNVFGQHSELIPFSSGRRSCPGIALALQI 479

Query: 381  MQLTLARLLQGFNLVSPTNEPVDMTEAAGISLHRKYPFEVVLTPRLPCKLY 229
            + LT+ARLLQG+++ +P N PVDMTE  G+++ ++ P EV+LTPRLP  LY
Sbjct: 480  LHLTVARLLQGYDMTTPLNAPVDMTEGIGLAMPKETPLEVMLTPRLPSLLY 530


>ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinifera]
            gi|302142392|emb|CBI19595.3| unnamed protein product
            [Vitis vinifera]
          Length = 527

 Score =  525 bits (1352), Expect = e-146
 Identities = 270/522 (51%), Positives = 363/522 (69%), Gaps = 5/522 (0%)
 Frame = -3

Query: 1779 LQLIAGFVALVLFYTLWSSCTRNHDEK--KKTQAPKPNGAWPIIGHIPLLAS-TKPAWKI 1609
            L L A FV L L Y  ++   R    K  K   AP+P GAWPIIGH+ LL    +  ++ 
Sbjct: 5    LHLPAFFVFLSLIYVFYAMLGRKKIIKSSKSRDAPEPGGAWPIIGHLHLLGGGDQLLYRT 64

Query: 1608 LADMADKCGPVFRIQLGWQNAVVVSSKEAVMQIFTTNDNNFMTRPTSLALKYMGYNGAFF 1429
            L  MADK GP F I+LG + A VVSS E   + FT ND    TRPT++A K+MGYN A F
Sbjct: 65   LGAMADKYGPAFNIRLGSRRAFVVSSWEVAKECFTINDKALATRPTTVAAKHMGYNYAVF 124

Query: 1428 AFSPYSTLYREMRKVSIFEVLSNSRMELLKPLRASEMTTCIKELYSLCCKNGIEVPVKLN 1249
             F+PYS  +REMRK++  E+LSN R+E+LK +R SE+   I+ELY L  KN    P+ + 
Sbjct: 125  GFAPYSPFWREMRKIATLELLSNRRLEILKHVRTSEVDMGIRELYGLWVKNSSR-PLLVE 183

Query: 1248 IEKWVQQVIINLVIQMLARKRYSSIGEGETEVESRRFKKAFEEFFILAGAFDLSDVIPFI 1069
            + +W++ + +N++++M+A KRY          E+RR +KA  +FF L G F +SD +PF+
Sbjct: 184  LNRWLEDMTLNVIVRMVAGKRYFGAAAASDSSEARRCQKAISQFFRLIGIFVVSDALPFL 243

Query: 1068 EWMDLQGNRRAMIRTAKELDVFMSSWIDEHKKSREQDQLKED--RDFIDVMISLFPEPDA 895
             W+DLQG+ RAM  TAKELD  +  W++EH++ R    +K +  +DFIDVM+SL  E   
Sbjct: 244  WWLDLQGHERAMKTTAKELDSILEGWLEEHRQRRVSSLIKAEGEQDFIDVMLSLQEEGRL 303

Query: 894  FVHGYKSTEVIKATVMSVIMGGSDAPAITLTWALSLLLNNKNVLEKAQQELDDHIGKERW 715
                Y S   IK+T +++I+GGSD  A TLTWA+SLLLNN++ L+KAQ+ELD  +G ER 
Sbjct: 304  SGFQYDSETSIKSTCLALILGGSDTTAGTLTWAISLLLNNRHALKKAQEELDLCVGMERQ 363

Query: 714  VEESDIRHLVYLQAILKETLRLYPGGPLGVPRKAKEDCTVAGYHVPKGTQLWVNIWKLHR 535
            VEESD+++LVYLQAI+KETLRLYP GPL  PR+A +DCTVAGY+VP GT+L VNIWKL R
Sbjct: 364  VEESDVKNLVYLQAIIKETLRLYPAGPLLGPREALDDCTVAGYNVPAGTRLIVNIWKLQR 423

Query: 534  DSESWTDPYEFQPERFLTSHAGVDVRGQQFEYIPYSSGRRSCPGITASMQMMQLTLARLL 355
            D   WT+P  FQPERFL +HA VDV+GQQFE +P+ SGRRSCPG++ ++Q++ LTLARLL
Sbjct: 424  DPSVWTNPCAFQPERFLNAHADVDVKGQQFELMPFGSGRRSCPGVSFALQVLHLTLARLL 483

Query: 354  QGFNLVSPTNEPVDMTEAAGISLHRKYPFEVVLTPRLPCKLY 229
              F L +P ++PVDMTE++G+++ +  P EV+LTPRL  KLY
Sbjct: 484  HAFELSTPVDQPVDMTESSGLTIPKATPLEVLLTPRLNSKLY 525


>ref|XP_002274740.1| PREDICTED: cytochrome P450 82C4 [Vitis vinifera]
          Length = 518

 Score =  523 bits (1347), Expect = e-146
 Identities = 269/523 (51%), Positives = 361/523 (69%)
 Frame = -3

Query: 1797 MDAVGLLQLIAGFVALVLFYTLWSSCTRNHDEKKKTQAPKPNGAWPIIGHIPLLASTKPA 1618
            MD    L  I+G + LVL Y +W   T     K ++ AP+P+GAWP +GH+PLL    P 
Sbjct: 1    MDTPSQLVAISGILGLVLLYGVWRVMTLAGKSKGRS-APEPSGAWPFLGHLPLLRGQTPI 59

Query: 1617 WKILADMADKCGPVFRIQLGWQNAVVVSSKEAVMQIFTTNDNNFMTRPTSLALKYMGYNG 1438
            ++ L  MADK GPVF I+LG   A+VVSS+EAV + FTTND  F +RP+S A K +GYN 
Sbjct: 60   FRTLGAMADKHGPVFMIRLGVHRALVVSSREAVKECFTTNDKVFASRPSSSAGKLLGYNY 119

Query: 1437 AFFAFSPYSTLYREMRKVSIFEVLSNSRMELLKPLRASEMTTCIKELYSLCCKNGIEVPV 1258
            A F F+PY  L+REMRK+S+ E+LS  R++ LK ++ SE+   IK+LYSL   +    PV
Sbjct: 120  AGFGFAPYGALWREMRKLSMMEILSARRLDALKHVQISELDLSIKDLYSLGKGSDWVHPV 179

Query: 1257 KLNIEKWVQQVIINLVIQMLARKRYSSIGEGETEVESRRFKKAFEEFFILAGAFDLSDVI 1078
            K+ + +W Q +  N+V++M+A KRY +   G    E+RR     +EF  L+GAF LSD I
Sbjct: 180  KVVMSEWFQHLSFNIVLKMIAGKRYFNTS-GHGIEEARRAIATIQEFLSLSGAFVLSDAI 238

Query: 1077 PFIEWMDLQGNRRAMIRTAKELDVFMSSWIDEHKKSREQDQLKEDRDFIDVMISLFPEPD 898
            P +EWMDLQG   +M R AKE+D  +  W++EH+  R   +    +DFIDVM+S+  +  
Sbjct: 239  PGVEWMDLQGYLGSMKRVAKEVDSLVGGWVEEHE-IRLNSEGSRMQDFIDVMLSVLEDTS 297

Query: 897  AFVHGYKSTEVIKATVMSVIMGGSDAPAITLTWALSLLLNNKNVLEKAQQELDDHIGKER 718
             F H  ++  VIKAT++ +I+GG++  A T TW LS LLNNK+ L++AQ+ELD  +G+ R
Sbjct: 298  MFGHSRET--VIKATIVILIVGGTETVATTSTWLLSALLNNKHALKRAQEELDLKVGRGR 355

Query: 717  WVEESDIRHLVYLQAILKETLRLYPGGPLGVPRKAKEDCTVAGYHVPKGTQLWVNIWKLH 538
            WVEESDI +L+YLQA++KETLRLY   PL VP +A EDC VAGYH+PKGT+L+VN WKLH
Sbjct: 356  WVEESDIPNLLYLQAVIKETLRLYTAAPLSVPHEAMEDCHVAGYHIPKGTRLFVNAWKLH 415

Query: 537  RDSESWTDPYEFQPERFLTSHAGVDVRGQQFEYIPYSSGRRSCPGITASMQMMQLTLARL 358
            RD   W+DP +FQPERFLTSHA +DV GQ FE IP+ SGRRSCPGIT +++++ L + RL
Sbjct: 416  RDPSVWSDPEDFQPERFLTSHADLDVLGQHFELIPFGSGRRSCPGITMALKLLPLVIGRL 475

Query: 357  LQGFNLVSPTNEPVDMTEAAGISLHRKYPFEVVLTPRLPCKLY 229
            LQGF+L +P N PVDM E   I+L +  P EV+LTPRLP + Y
Sbjct: 476  LQGFDLSTPLNAPVDMREGLSITLAKLTPLEVILTPRLPSQFY 518