BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00022854
         (1016 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   300   4e-79
ref|XP_003521174.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   268   2e-69
ref|XP_002308030.1| predicted protein [Populus trichocarpa] gi|2...   255   1e-65
ref|XP_003553411.1| PREDICTED: LOW QUALITY PROTEIN: cytoplasmic ...   238   1e-60
ref|XP_002867047.1| hypothetical protein ARALYDRAFT_328161 [Arab...   234   3e-59

>ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Vitis vinifera]
            gi|297739656|emb|CBI29838.3| unnamed protein product
            [Vitis vinifera]
          Length = 468

 Score =  300 bits (768), Expect = 4e-79
 Identities = 153/224 (68%), Positives = 178/224 (79%), Gaps = 3/224 (1%)
 Frame = -3

Query: 1014 IAATVKGQGYSLAADIQYVDARWQIPVVLPLRDCLIQELNMLCRLDSLKTLDLYSGSSTG 835
            I+ATVKGQGYSL ADIQYVDARW+IPVVLPLRDCL QELNMLCRLD LKT+++ +   +G
Sbjct: 248  ISATVKGQGYSLPADIQYVDARWEIPVVLPLRDCLAQELNMLCRLDGLKTMEVLNSPHSG 307

Query: 834  VNGLISSFVKLLQEENPSRESTIVRTAGKLTPFHFNKIPEEGNDYNLQLASHRRRKKFNL 655
            +NGL++SFV LLQEENPSRE TIVRTAGKLTPFHFN+IP E +D N+ LAS RR+KKFNL
Sbjct: 308  INGLVASFVTLLQEENPSRECTIVRTAGKLTPFHFNQIP-ESDDSNVHLASRRRQKKFNL 366

Query: 654  KSEEHLPPDSFCPICNSPLKKH---NFSSLPFENGHASPETFRSKCCSSCQFQILPKEPL 484
            K  E +PP+S+CPICNSPL K    N SSL  +  +A  + F + CC SCQFQILPKEP+
Sbjct: 367  KPNESVPPESYCPICNSPLSKSDHINLSSLGSDQTNA--DIFGATCCPSCQFQILPKEPV 424

Query: 483  HMKHFYSLLPQPITDRVIDGSCSNLRSIREQIQDCLLSDSEDGT 352
             M+HFYSLLPQ    +  D S S  R +REQIQD LLSDSEDGT
Sbjct: 425  SMEHFYSLLPQQFVVQAKDRSFSMQRQLREQIQDFLLSDSEDGT 468


>ref|XP_003521174.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Glycine max]
          Length = 458

 Score =  268 bits (684), Expect = 2e-69
 Identities = 134/221 (60%), Positives = 163/221 (73%)
 Frame = -3

Query: 1014 IAATVKGQGYSLAADIQYVDARWQIPVVLPLRDCLIQELNMLCRLDSLKTLDLYSGSSTG 835
            I+ATVKGQGYSL ADIQYVDARW++PVVLPLRDC  QE+NMLC LD LKT+ L +   + 
Sbjct: 239  ISATVKGQGYSLPADIQYVDARWEVPVVLPLRDCFAQEINMLCHLDGLKTVKLSTDLCSS 298

Query: 834  VNGLISSFVKLLQEENPSRESTIVRTAGKLTPFHFNKIPEEGNDYNLQLASHRRRKKFNL 655
            +NGL+SSFV LLQEENPSRESTIVRTAGKLTPF FN+IPE   D N+ LA+ RR+K++NL
Sbjct: 299  INGLVSSFVALLQEENPSRESTIVRTAGKLTPFQFNRIPEI-IDGNVPLATRRRQKRYNL 357

Query: 654  KSEEHLPPDSFCPICNSPLKKHNFSSLPFENGHASPETFRSKCCSSCQFQILPKEPLHMK 475
            KS E +  +SFCP+CNSPL K   +     + H S + F + CCSSC+FQILP + + M+
Sbjct: 358  KSNESVSSESFCPLCNSPLDKSEITDWSNIDSHRSSDIFYNTCCSSCRFQILPSDSMAME 417

Query: 474  HFYSLLPQPITDRVIDGSCSNLRSIREQIQDCLLSDSEDGT 352
             FY  LPQ +  R    +  NL  +REQIQD LLSD ED T
Sbjct: 418  QFYMDLPQSVVGRAKQANNGNLSLLREQIQDYLLSDGEDET 458


>ref|XP_002308030.1| predicted protein [Populus trichocarpa] gi|222854006|gb|EEE91553.1|
            predicted protein [Populus trichocarpa]
          Length = 443

 Score =  255 bits (651), Expect = 1e-65
 Identities = 128/207 (61%), Positives = 158/207 (76%), Gaps = 1/207 (0%)
 Frame = -3

Query: 1014 IAATVKGQGYSLAADIQYVDARWQIPVVLPLRDCLIQELNMLCRLDSLKTLDLYSGSSTG 835
            +AATVKGQGYSL+ADIQYVDARW+IPVVLPLRDC  QELN+LC+LD LKTL  +  + +G
Sbjct: 240  LAATVKGQGYSLSADIQYVDARWEIPVVLPLRDCTSQELNLLCQLDGLKTLQPFDSTPSG 299

Query: 834  VNGLISSFVKLLQEENPSRESTIVRTAGKLTPFHFNKIPEEGNDYNLQLASHRRRKKFNL 655
            +NGLISSFV +LQEEN SRE TI+RTAGKLTPF FN+IPE  N  N+ LA  RR+K++NL
Sbjct: 300  INGLISSFVNVLQEENSSRECTILRTAGKLTPFEFNRIPETDN-CNVPLAIRRRQKRYNL 358

Query: 654  KSEEHLPPDSFCPICNSPLKKHNFSSLPFEN-GHASPETFRSKCCSSCQFQILPKEPLHM 478
            K++E +  + FCPICN+PLK  NF+SL   +        F + CCSSCQFQI+PK+P  M
Sbjct: 359  KTKESISSELFCPICNAPLK--NFNSLNLSSLKSCQSSRFNAACCSSCQFQIIPKDPSSM 416

Query: 477  KHFYSLLPQPITDRVIDGSCSNLRSIR 397
             +F SLLPQ + DR   G+CSNL  +R
Sbjct: 417  GNFCSLLPQQVVDRAKHGNCSNLSLLR 443


>ref|XP_003553411.1| PREDICTED: LOW QUALITY PROTEIN: cytoplasmic tRNA 2-thiolation protein
            2-like, partial [Glycine max]
          Length = 389

 Score =  238 bits (608), Expect = 1e-60
 Identities = 119/206 (57%), Positives = 147/206 (71%)
 Frame = -3

Query: 1014 IAATVKGQGYSLAADIQYVDARWQIPVVLPLRDCLIQELNMLCRLDSLKTLDLYSGSSTG 835
            I+ATVKGQGYSL ADIQYVDARW++PVVLPLRDC  QE+NMLCRLD LKT+ L +   + 
Sbjct: 185  ISATVKGQGYSLPADIQYVDARWEVPVVLPLRDCFAQEINMLCRLDGLKTVKLSTDLCSS 244

Query: 834  VNGLISSFVKLLQEENPSRESTIVRTAGKLTPFHFNKIPEEGNDYNLQLASHRRRKKFNL 655
            +NGL+SSFV LLQEENPSRES+IVRTAGKLTPF FN+IPE   D N+ L + R +K++NL
Sbjct: 245  INGLVSSFVALLQEENPSRESSIVRTAGKLTPFQFNRIPEI-IDSNVPLTTRRCQKRYNL 303

Query: 654  KSEEHLPPDSFCPICNSPLKKHNFSSLPFENGHASPETFRSKCCSSCQFQILPKEPLHMK 475
            KS E +  +SFCP+CNSPL K         + H S + F + CCSSC+FQILP +   M+
Sbjct: 304  KSNESVSSESFCPLCNSPLDKSEIVDWSNIDSHRSSDIFYNTCCSSCRFQILPSDSTTME 363

Query: 474  HFYSLLPQPITDRVIDGSCSNLRSIR 397
             FY   PQ +  R    +  NL  +R
Sbjct: 364  QFYMDFPQSVVSRPKQANNGNLSLLR 389


>ref|XP_002867047.1| hypothetical protein ARALYDRAFT_328161 [Arabidopsis lyrata subsp.
            lyrata] gi|297312883|gb|EFH43306.1| hypothetical protein
            ARALYDRAFT_328161 [Arabidopsis lyrata subsp. lyrata]
          Length = 459

 Score =  234 bits (597), Expect = 3e-59
 Identities = 117/220 (53%), Positives = 159/220 (72%), Gaps = 1/220 (0%)
 Frame = -3

Query: 1014 IAATVKGQGYSLAADIQYVDARWQIPVVLPLRDCLIQELNMLCRLDSLKTLDLYSGSSTG 835
            ++ATVKG+GYSL+ADIQ+VDARW++P+VLPLRDC+  E+  LC LD LKT++L   S  G
Sbjct: 240  LSATVKGRGYSLSADIQHVDARWKVPIVLPLRDCVRLEITRLCHLDGLKTVELACRSQCG 299

Query: 834  VNGLISSFVKLLQEENPSRESTIVRTAGKLTPFHFNKIPEEGNDYNLQLASHRRRKKFNL 655
            +N L+SSFV LLQEENPSRE TIVRTA KLTPF+FNKIPE  +D N+ +A+ RR K+FNL
Sbjct: 300  INDLVSSFVALLQEENPSRECTIVRTAAKLTPFYFNKIPET-DDSNVPMATQRRLKRFNL 358

Query: 654  KSEEHLPPDSFCPICNSPLKKHNFSSL-PFENGHASPETFRSKCCSSCQFQILPKEPLHM 478
            K +  +  ++FCPICN PL + + S L  FE G  + +   + CCSSC+FQILP++  ++
Sbjct: 359  KYDGSMTTEAFCPICNGPLNRSDSSDLDTFEEGQEA-DVLYAACCSSCRFQILPEDGSYL 417

Query: 477  KHFYSLLPQPITDRVIDGSCSNLRSIREQIQDCLLSDSED 358
            + F S LP  +  +V      +   +RE+I+DCLL D E+
Sbjct: 418  EQFSSFLPDHMISQVKHQKVDSQAHLREKIKDCLLLDDEE 457