BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00022779
         (1197 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002267695.2| PREDICTED: folylpolyglutamate synthase-like ...   308   e-100
emb|CAN76202.1| hypothetical protein VITISV_018995 [Vitis vinifera]   279   4e-91
ref|XP_002513590.1| tetrahydrofolylpolyglutamate synthase, putat...   265   2e-86
ref|XP_003544122.1| PREDICTED: LOW QUALITY PROTEIN: folylpolyglu...   263   1e-85
ref|XP_004149457.1| PREDICTED: folylpolyglutamate synthase-like ...   264   5e-85

>ref|XP_002267695.2| PREDICTED: folylpolyglutamate synthase-like [Vitis vinifera]
            gi|302143688|emb|CBI22549.3| unnamed protein product
            [Vitis vinifera]
          Length = 536

 Score =  308 bits (789), Expect(2) = e-100
 Identities = 163/291 (56%), Positives = 210/291 (72%)
 Frame = -2

Query: 1046 LGGPFLPEIESILRAKASSMGSPVVSVSDPGNKSIIRGISDVGGKPCQCCDIVLEVERDY 867
            LGGPFLP IE I   KASSM SPVVS S PGN+S ++G+S   GKP Q CDIV+EVERD+
Sbjct: 237  LGGPFLPHIEHIFLDKASSMCSPVVSASGPGNRSAVKGVSKSNGKPFQSCDIVIEVERDF 296

Query: 866  KLFFQMPNVHLCMLGPHQLKNXXXXXXXXXXLRDQGWSISDESIRAGLEQTKLLGRSQFL 687
            KLF ++ +V L MLG HQL+N          LRD+GW ISDESI AGLE   LLGRSQFL
Sbjct: 297  KLFIELFDVKLQMLGIHQLQNAATATCVALCLRDKGWRISDESIHAGLEHAYLLGRSQFL 356

Query: 686  TSEETETLGLQEAKVLLDGAHTKESAKALADTIQMTFPKVRLVLVLAMASDKDHLSFAKE 507
            TS E ETLGL  A ++LDGAHTKESAKAL DTIQMTFP+ RL L++AMASDKDH++FA+E
Sbjct: 357  TSTEAETLGLPGATIMLDGAHTKESAKALVDTIQMTFPEARLALIVAMASDKDHMAFARE 416

Query: 506  LLSVRQLEAVILTEVSIGGDNFRSTASSSLKDSWIQASKDVGVEIFDFGLTDCISFSEYE 327
             LS  QLEAV LTEV+I G   R+T++S L+D WIQASK++G+     G+ +     E +
Sbjct: 417  FLSGGQLEAVFLTEVNIAGAKSRTTSASMLRDCWIQASKELGINTLHDGMEEYQKLFENQ 476

Query: 326  ANYIRKKQEYGKLALVESSFQDSIKIVDQMLRTRTKDKTDNMVVVTGSLHI 174
            +     + ++  +   E+S   S+++ +Q+LR RT+D+T +++V+TGSLHI
Sbjct: 477  SFCSAGESKHKTILAAENSLLVSLRVGNQILRARTRDQT-SIIVITGSLHI 526



 Score = 83.6 bits (205), Expect(2) = e-100
 Identities = 44/51 (86%), Positives = 48/51 (94%)
 Frame = -1

Query: 1197 GARDATNILSSSMLAAAVITTIGEEHLAALGGSLESIAIAKSGIIKRNRPV 1045
            GARDATNI+SSS LAAAVITTIGEEHLAALGGSLESIA+AKSGIIK+  P+
Sbjct: 185  GARDATNIISSSGLAAAVITTIGEEHLAALGGSLESIAMAKSGIIKQGCPL 235


>emb|CAN76202.1| hypothetical protein VITISV_018995 [Vitis vinifera]
          Length = 637

 Score =  279 bits (714), Expect(2) = 4e-91
 Identities = 154/291 (52%), Positives = 197/291 (67%)
 Frame = -2

Query: 1046 LGGPFLPEIESILRAKASSMGSPVVSVSDPGNKSIIRGISDVGGKPCQCCDIVLEVERDY 867
            LGGPFLP IE I   KASSM SPVVS S PGN+S ++G+S   GKP Q CDIV+EVERD+
Sbjct: 237  LGGPFLPHIEHIFLDKASSMCSPVVSASGPGNRSAVKGVSKSNGKPFQSCDIVIEVERDF 296

Query: 866  KLFFQMPNVHLCMLGPHQLKNXXXXXXXXXXLRDQGWSISDESIRAGLEQTKLLGRSQFL 687
            KLF ++ +V L MLG HQL+N          LRD+GW ISDESI AGLE   LLGRSQFL
Sbjct: 297  KLFIELFDVKLQMLGIHQLQNAATATCVALCLRDKGWRISDESIHAGLEHAYLLGRSQFL 356

Query: 686  TSEETETLGLQEAKVLLDGAHTKESAKALADTIQMTFPKVRLVLVLAMASDKDHLSFAKE 507
            TS E ETLGL  A ++LDGAHTKESAKAL DTIQMTFP+ RL L++AMAS          
Sbjct: 357  TSTEAETLGLPGATIMLDGAHTKESAKALVDTIQMTFPEARLALIVAMASG--------- 407

Query: 506  LLSVRQLEAVILTEVSIGGDNFRSTASSSLKDSWIQASKDVGVEIFDFGLTDCISFSEYE 327
                 QLEAV LTEV+I G N R+T++S L+D WIQASK++G+     G+ +     E +
Sbjct: 408  -----QLEAVFLTEVNIAGANSRTTSASMLRDCWIQASKELGINTLHDGMEEYQKLFENQ 462

Query: 326  ANYIRKKQEYGKLALVESSFQDSIKIVDQMLRTRTKDKTDNMVVVTGSLHI 174
            +     + ++  +   E+S   S+++ +Q+LR R +D+T  ++V+TGSLHI
Sbjct: 463  SFCSAGESKHKTILAAENSLXVSLRVGNQILRARXRDQT-XIIVITGSLHI 512



 Score = 83.6 bits (205), Expect(2) = 4e-91
 Identities = 44/51 (86%), Positives = 48/51 (94%)
 Frame = -1

Query: 1197 GARDATNILSSSMLAAAVITTIGEEHLAALGGSLESIAIAKSGIIKRNRPV 1045
            GARDATNI+SSS LAAAVITTIGEEHLAALGGSLESIA+AKSGIIK+  P+
Sbjct: 185  GARDATNIISSSGLAAAVITTIGEEHLAALGGSLESIAMAKSGIIKQGCPL 235


>ref|XP_002513590.1| tetrahydrofolylpolyglutamate synthase, putative [Ricinus communis]
            gi|223547498|gb|EEF48993.1| tetrahydrofolylpolyglutamate
            synthase, putative [Ricinus communis]
          Length = 552

 Score =  265 bits (677), Expect(2) = 2e-86
 Identities = 145/293 (49%), Positives = 200/293 (68%), Gaps = 2/293 (0%)
 Frame = -2

Query: 1046 LGGPFLPEIESILRAKASSMGSPVVSVSDPGNKSIIRGISDVGGKPCQCCDIVLEVERDY 867
            LGGPFLP+IE ILR KAS M +PVVS SD G ++ I+G+S   G+ CQ CDI + V++  
Sbjct: 253  LGGPFLPDIERILRDKASLMLAPVVSASDSGIRTTIKGLSMFDGRHCQLCDIKIRVKKAL 312

Query: 866  KLFFQMPNVHLCMLGPHQLKNXXXXXXXXXXLRDQGWSISDESIRAGLEQTKLLGRSQFL 687
             LF ++ ++ + MLG HQL+N          LRDQGW ISD SIR GLE T LLGRSQFL
Sbjct: 313  NLFIELSDLEMRMLGSHQLQNAATAACAALCLRDQGWKISDASIRLGLENTCLLGRSQFL 372

Query: 686  TSEETETLGLQEAKVLLDGAHTKESAKALADTIQMTFPKVRLVLVLAMASDKDHLSFAKE 507
             S++ E LG+  A +LLDGAHTKESAKAL DT+++TFP+ RL L++AMASDKDHL+FA E
Sbjct: 373  ASKDAEVLGVPGATILLDGAHTKESAKALVDTVRLTFPEARLALIVAMASDKDHLAFAGE 432

Query: 506  LLSVRQLEAVILTEVSIGGDNFRSTASSSLKDSWIQASKDVGVEIFDFGLTDCISFSEYE 327
             L  ++LEAV LTE  I G   R+TA+  L+D W+QA +++G+     G+ +     E E
Sbjct: 433  FLLDKKLEAVFLTEAEIAGGKSRTTAAPLLRDYWVQACEELGINTLHDGMAEYRELLENE 492

Query: 326  ANYIRKKQEY-GKLAL-VESSFQDSIKIVDQMLRTRTKDKTDNMVVVTGSLHI 174
              +I  ++   GK+ L   +S + S+++  + LR RT +++ +++VVTGSLHI
Sbjct: 493  --FIHSERHLGGKIILATNNSLEISLRMASEFLRGRTGNQS-SVLVVTGSLHI 542



 Score = 82.0 bits (201), Expect(2) = 2e-86
 Identities = 41/51 (80%), Positives = 46/51 (90%)
 Frame = -1

Query: 1197 GARDATNILSSSMLAAAVITTIGEEHLAALGGSLESIAIAKSGIIKRNRPV 1045
            GARDATNIL SS LA ++ITTIGEEHL ALGGSLESIA+AK+GIIKR RP+
Sbjct: 201  GARDATNILCSSQLATSIITTIGEEHLMALGGSLESIAMAKAGIIKRGRPL 251


>ref|XP_003544122.1| PREDICTED: LOW QUALITY PROTEIN: folylpolyglutamate synthase-like
            [Glycine max]
          Length = 536

 Score =  263 bits (671), Expect(2) = 1e-85
 Identities = 146/292 (50%), Positives = 194/292 (66%), Gaps = 1/292 (0%)
 Frame = -2

Query: 1046 LGGPFLPEIESILRAKASSMGSPVVSVSDPGNKSIIRGISDVGGKPCQCCDIVLEVERDY 867
            LGGPF+P IE I+R KA +M SPVVS  D GNK  ++  S + G+PCQ CDI+++V +D 
Sbjct: 237  LGGPFVPHIERIIRDKAVAMNSPVVSAYDTGNKCTMKSFSILNGRPCQICDILIQVVKDM 296

Query: 866  KLFFQMPNVHLCMLGPHQLKNXXXXXXXXXXLRDQGWSISDESIRAGLEQTKLLGRSQFL 687
            K+  ++ +V L MLG HQL+N          LR+ GWSISD+SIR GLE T LLGRSQFL
Sbjct: 297  KMSCKLHDVKLQMLGDHQLQNAATATCVALVLRNLGWSISDKSIRYGLEDTYLLGRSQFL 356

Query: 686  TSEETETLGLQEAKVLLDGAHTKESAKALADTIQMTFPKVRLVLVLAMASDKDHLSFAKE 507
            TSEE E LGL  + VLLDGAHTKESAKAL +TI+M FPK +LV V+AMASDKDH+ FA+E
Sbjct: 357  TSEEAEVLGLTGSTVLLDGAHTKESAKALMNTIRMAFPKAQLVFVVAMASDKDHVGFARE 416

Query: 506  LLSVRQLEAVILTEVSIGGDNFRSTASSSLKDSWIQASKDVGVEIFDFGLTDCISFSEYE 327
            +LS   +E V+LTE +I G   R+T +S L+DSWI+AS ++G+ I   G+ +     + +
Sbjct: 417  ILSGVHVETVVLTEAAIAGGVTRTTPASLLRDSWIKASGELGIGIVHDGMAEYSELLKEQ 476

Query: 326  ANYIRKKQEYGKLAL-VESSFQDSIKIVDQMLRTRTKDKTDNMVVVTGSLHI 174
                      GK  L  ES  +  ++  +Q+L  R  +K   ++V TGSLHI
Sbjct: 477  PVGSESNLGDGKTVLATESCLKGCLRTANQILSRRGDEK--RVIVFTGSLHI 526



 Score = 81.6 bits (200), Expect(2) = 1e-85
 Identities = 40/51 (78%), Positives = 49/51 (96%)
 Frame = -1

Query: 1197 GARDATNILSSSMLAAAVITTIGEEHLAALGGSLESIAIAKSGIIKRNRPV 1045
            GARDATNI+SSS L A+VITT+GEEHLAALGGSLE+IA+AK+GIIK++RP+
Sbjct: 185  GARDATNIISSSGLVASVITTVGEEHLAALGGSLETIAMAKAGIIKQDRPL 235


>ref|XP_004149457.1| PREDICTED: folylpolyglutamate synthase-like [Cucumis sativus]
            gi|449521387|ref|XP_004167711.1| PREDICTED:
            folylpolyglutamate synthase-like [Cucumis sativus]
          Length = 548

 Score =  264 bits (674), Expect(2) = 5e-85
 Identities = 146/291 (50%), Positives = 196/291 (67%), Gaps = 1/291 (0%)
 Frame = -2

Query: 1046 LGGPFLPEIESILRAKASSMGSPVVSVSDPGNKSIIRGISDVGGKPCQCCDIVLEVERDY 867
            LGGPFLP IE ILR KA SM SPV+S SDPGN+S I+G++ + G   QCCDIV++++ + 
Sbjct: 260  LGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNE- 318

Query: 866  KLFFQMPNVHLCMLGPHQLKNXXXXXXXXXXLRDQGWSISDESIRAGLEQTKLLGRSQFL 687
              F ++ +V+L MLGPHQL+N          LR+ GW ISD SIR+GLEQT L+GRS FL
Sbjct: 319  --FIELLDVNLRMLGPHQLQNAATATCVILTLRNLGWRISDASIRSGLEQTFLIGRSHFL 376

Query: 686  TSEETETLGLQEAKVLLDGAHTKESAKALADTIQMTFPKVRLVLVLAMASDKDHLSFAKE 507
             + E E LGL  A +LLDGAHTK+SAKAL DTIQM FP+ +L LV+AMASDK+H+ FA+E
Sbjct: 377  AAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLALVVAMASDKNHVGFARE 436

Query: 506  LLSVRQLEAVILTEVSIGGDNFRSTASSSLKDSWIQASKDVGVEIFDFGLTDCISFSEYE 327
             L   +LE+V+LTE  IGG   R+T+++ L+D WIQAS ++G+          IS    +
Sbjct: 437  FLQGGKLESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIP---------ISLETKD 487

Query: 326  AN-YIRKKQEYGKLALVESSFQDSIKIVDQMLRTRTKDKTDNMVVVTGSLH 177
            A  +   K     +   E+S   +IKI  ++L+ RTK +   +VVV+GSLH
Sbjct: 488  AEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGR-QGLVVVSGSLH 537



 Score = 78.6 bits (192), Expect(2) = 5e-85
 Identities = 41/50 (82%), Positives = 45/50 (90%)
 Frame = -1

Query: 1197 GARDATNILSSSMLAAAVITTIGEEHLAALGGSLESIAIAKSGIIKRNRP 1048
            GARDATNI+ SS LAAAVIT+IGEEH+AALGGSLESIA AK+GIIKR  P
Sbjct: 208  GARDATNIICSSELAAAVITSIGEEHVAALGGSLESIATAKAGIIKRGCP 257