BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00022705
         (819 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002312025.1| predicted protein [Populus trichocarpa] gi|2...   261   5e-84
emb|CBI31488.3| unnamed protein product [Vitis vinifera]              276   6e-84
ref|XP_003591952.1| RNA pseudourine synthase [Medicago truncatul...   248   2e-83
ref|XP_002276664.1| PREDICTED: RNA pseudourine synthase 5-like [...   274   2e-83
ref|XP_003556222.1| PREDICTED: RNA pseudourine synthase 5-like [...   247   4e-82

>ref|XP_002312025.1| predicted protein [Populus trichocarpa] gi|222851845|gb|EEE89392.1|
           predicted protein [Populus trichocarpa]
          Length = 377

 Score =  261 bits (666), Expect(2) = 5e-84
 Identities = 131/187 (70%), Positives = 158/187 (84%)
 Frame = -3

Query: 817 LIALNKPSGLQVLPGGLFQHRTVLTQLQWYARKQISLVARQETNPVPVHRLGRGTSGILL 638
           ++ALNKP GLQVLPGGLFQ RTVL QLQW A K  S +A Q ++PVPVHRLGRGTSGILL
Sbjct: 112 MVALNKPPGLQVLPGGLFQQRTVLMQLQWRASKGSSSLAIQGSHPVPVHRLGRGTSGILL 171

Query: 637 CAKTKLAKSRISAYFADGTSVVGSSRIGDTDISKTGKISKTYRALASGIIGENEVIINQP 458
           CAKTK+AK+ ++AYFADGTS++G S   + + SK  KISK YRAL +GI+ ++++II QP
Sbjct: 172 CAKTKVAKTSLAAYFADGTSLIGDSSNANMEQSKRRKISKIYRALVNGILNQDKIIIKQP 231

Query: 457 IGMLQYPGVAKGLYVASPSGKLALSKVCVLERNMQCDRTLVEVEIQSGRPHQIRIHLSFI 278
           IG ++YPGVAKGLYVASPSGK ALSKV VLER+ Q ++TLV+VEI+SGRPHQIRIHLSFI
Sbjct: 232 IGTMRYPGVAKGLYVASPSGKPALSKVEVLERDSQLNQTLVQVEIESGRPHQIRIHLSFI 291

Query: 277 GHPLIVD 257
           GHPL+ D
Sbjct: 292 GHPLLGD 298



 Score = 77.4 bits (189), Expect(2) = 5e-84
 Identities = 34/54 (62%), Positives = 41/54 (75%), Gaps = 1/54 (1%)
 Frame = -2

Query: 266 DSGYRRPQNPVPGDCGYHLHAHRLVFSHPL-TSQITEVVAPLPSILQTQEESRE 108
           D GY RP  PVPGDCGY+LHAH+LV SHP    Q+ ++ APLPSIL+TQ E+ E
Sbjct: 322 DGGYERPAKPVPGDCGYYLHAHQLVLSHPTKNEQVIKITAPLPSILRTQAETEE 375


>emb|CBI31488.3| unnamed protein product [Vitis vinifera]
          Length = 347

 Score =  276 bits (705), Expect(2) = 6e-84
 Identities = 140/187 (74%), Positives = 160/187 (85%)
 Frame = -3

Query: 817 LIALNKPSGLQVLPGGLFQHRTVLTQLQWYARKQISLVARQETNPVPVHRLGRGTSGILL 638
           +IALNKPSGLQVLPGGLFQ RTVLTQLQW A KQ SL A QE++ VPVHRLGRGTSGILL
Sbjct: 102 VIALNKPSGLQVLPGGLFQQRTVLTQLQWRANKQNSLPACQESHSVPVHRLGRGTSGILL 161

Query: 637 CAKTKLAKSRISAYFADGTSVVGSSRIGDTDISKTGKISKTYRALASGIIGENEVIINQP 458
           CAKTKLAK+R++AYFADGTSVVG +R  D       KISK YRAL +GI+ ++E++I QP
Sbjct: 162 CAKTKLAKTRLAAYFADGTSVVGGNRDADMKSRTMRKISKIYRALVNGILDKDEIVIEQP 221

Query: 457 IGMLQYPGVAKGLYVASPSGKLALSKVCVLERNMQCDRTLVEVEIQSGRPHQIRIHLSFI 278
           IGM+QYPGVAKGLYVASPSGK ALSKV +LER++Q D  +V+VEIQSGRPHQIRIHLSFI
Sbjct: 222 IGMVQYPGVAKGLYVASPSGKPALSKVSILERDVQGDHMIVQVEIQSGRPHQIRIHLSFI 281

Query: 277 GHPLIVD 257
           GHPL+ D
Sbjct: 282 GHPLLGD 288



 Score = 62.0 bits (149), Expect(2) = 6e-84
 Identities = 24/33 (72%), Positives = 28/33 (84%)
 Frame = -2

Query: 266 DSGYRRPQNPVPGDCGYHLHAHRLVFSHPLTSQ 168
           D GY+RP  PVPGDCGYHLHAH+LV SHP T++
Sbjct: 312 DGGYQRPVKPVPGDCGYHLHAHQLVLSHPTTNE 344


>ref|XP_003591952.1| RNA pseudourine synthase [Medicago truncatula]
           gi|358349060|ref|XP_003638558.1| RNA pseudourine
           synthase [Medicago truncatula]
           gi|355481000|gb|AES62203.1| RNA pseudourine synthase
           [Medicago truncatula] gi|355504493|gb|AES85696.1| RNA
           pseudourine synthase [Medicago truncatula]
          Length = 371

 Score =  248 bits (632), Expect(2) = 2e-83
 Identities = 131/188 (69%), Positives = 156/188 (82%), Gaps = 1/188 (0%)
 Frame = -3

Query: 817 LIALNKPSGLQVLPGGLFQHRTVLTQLQWYARKQISLVARQETNPVPVHRLGRGTSGILL 638
           +IALNKPSGLQVLPGGLFQ RTVLTQLQW    + ++ A Q+ +PVPVHRLGRGTSGILL
Sbjct: 104 IIALNKPSGLQVLPGGLFQQRTVLTQLQWETNNKSTIEAHQKPHPVPVHRLGRGTSGILL 163

Query: 637 CAKTKLAKSRISAYFADGTSVVGSSRIGDTDISKTGKISKTYRALASGIIGENEVIINQP 458
           CAKTKLA++R+++YFADGTS +     G T+  + GKI+K YRAL SGII  ++V INQP
Sbjct: 164 CAKTKLARARLASYFADGTSRIEGK--GHTN-QELGKIAKIYRALVSGIINNDQVTINQP 220

Query: 457 IGMLQYPGVAKGLYVASPSGKLALSKVCVLERNMQCDRTLVE-VEIQSGRPHQIRIHLSF 281
           IGM++YPGVAKGLYVAS SGK ALS V VLERN++ + TLV+ V+IQSGRPHQIRIHLSF
Sbjct: 221 IGMVKYPGVAKGLYVASQSGKPALSVVHVLERNVKENSTLVQVVKIQSGRPHQIRIHLSF 280

Query: 280 IGHPLIVD 257
           IGHPL+ D
Sbjct: 281 IGHPLLGD 288



 Score = 88.6 bits (218), Expect(2) = 2e-83
 Identities = 37/51 (72%), Positives = 43/51 (84%)
 Frame = -2

Query: 266 DSGYRRPQNPVPGDCGYHLHAHRLVFSHPLTSQITEVVAPLPSILQTQEES 114
           D GY RP  PVPGDCGYHLHAHRL  SHP+T++I E++APLPSILQT EE+
Sbjct: 312 DGGYTRPSKPVPGDCGYHLHAHRLFLSHPITNEIIEMIAPLPSILQTHEEA 362


>ref|XP_002276664.1| PREDICTED: RNA pseudourine synthase 5-like [Vitis vinifera]
          Length = 348

 Score =  274 bits (700), Expect(2) = 2e-83
 Identities = 141/188 (75%), Positives = 163/188 (86%), Gaps = 1/188 (0%)
 Frame = -3

Query: 817 LIALNKPSGLQVLPGGLFQHRTVLTQLQWYARKQISLVARQETNPVPVHRLGRGTSGILL 638
           +IALNKPSGLQVLPGGLFQ RTVLTQLQW A KQ SL A QE++ VPVHRLGRGTSGILL
Sbjct: 102 VIALNKPSGLQVLPGGLFQQRTVLTQLQWRANKQNSLPACQESHSVPVHRLGRGTSGILL 161

Query: 637 CAKTKLAKSRISAYFADGTSVVGSSRIGDTDISKT-GKISKTYRALASGIIGENEVIINQ 461
           CAKTKLAK+R++AYFADGTSVVG +R+     S+T  KISK YRAL +GI+ ++E++I Q
Sbjct: 162 CAKTKLAKTRLAAYFADGTSVVGGNRLDADMKSRTMRKISKIYRALVNGILDKDEIVIEQ 221

Query: 460 PIGMLQYPGVAKGLYVASPSGKLALSKVCVLERNMQCDRTLVEVEIQSGRPHQIRIHLSF 281
           PIGM+QYPGVAKGLYVASPSGK ALSKV +LER++Q D  +V+VEIQSGRPHQIRIHLSF
Sbjct: 222 PIGMVQYPGVAKGLYVASPSGKPALSKVSILERDVQGDHMIVQVEIQSGRPHQIRIHLSF 281

Query: 280 IGHPLIVD 257
           IGHPL+ D
Sbjct: 282 IGHPLLGD 289



 Score = 62.0 bits (149), Expect(2) = 2e-83
 Identities = 24/33 (72%), Positives = 28/33 (84%)
 Frame = -2

Query: 266 DSGYRRPQNPVPGDCGYHLHAHRLVFSHPLTSQ 168
           D GY+RP  PVPGDCGYHLHAH+LV SHP T++
Sbjct: 313 DGGYQRPVKPVPGDCGYHLHAHQLVLSHPTTNE 345


>ref|XP_003556222.1| PREDICTED: RNA pseudourine synthase 5-like [Glycine max]
          Length = 371

 Score =  247 bits (630), Expect(2) = 4e-82
 Identities = 128/187 (68%), Positives = 154/187 (82%)
 Frame = -3

Query: 817 LIALNKPSGLQVLPGGLFQHRTVLTQLQWYARKQISLVARQETNPVPVHRLGRGTSGILL 638
           +IALNKPSGLQVLPGGL+Q RT+LTQLQW A  Q +    +  +PVPVHRLGRGTSGILL
Sbjct: 104 MIALNKPSGLQVLPGGLYQQRTILTQLQWEANNQGTCEMHKRLHPVPVHRLGRGTSGILL 163

Query: 637 CAKTKLAKSRISAYFADGTSVVGSSRIGDTDISKTGKISKTYRALASGIIGENEVIINQP 458
           CAKTKLA++R++++FADGTS VG  R  DT   + GKI+K YRAL SGI+  ++V INQP
Sbjct: 164 CAKTKLARARLASHFADGTSHVGGKR--DTK-QELGKIAKMYRALVSGIVENDKVTINQP 220

Query: 457 IGMLQYPGVAKGLYVASPSGKLALSKVCVLERNMQCDRTLVEVEIQSGRPHQIRIHLSFI 278
           IG+++YPGVAKGLYVAS SGK ALS V +LE N+Q + TLV+V+IQSGRPHQIRIHLSFI
Sbjct: 221 IGIVKYPGVAKGLYVASESGKPALSVVDILETNIQENSTLVQVKIQSGRPHQIRIHLSFI 280

Query: 277 GHPLIVD 257
           GHPL+ D
Sbjct: 281 GHPLLGD 287



 Score = 84.7 bits (208), Expect(2) = 4e-82
 Identities = 34/53 (64%), Positives = 44/53 (83%)
 Frame = -2

Query: 266 DSGYRRPQNPVPGDCGYHLHAHRLVFSHPLTSQITEVVAPLPSILQTQEESRE 108
           D GY+RP  PVPGDCGY+LHAH+LV SHP T+++ E++APLPS L T EE++E
Sbjct: 311 DGGYQRPTKPVPGDCGYYLHAHKLVLSHPTTNELIEIIAPLPSALHTAEEAKE 363