BLASTX nr result
ID: Bupleurum21_contig00022641
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00022641
(1328 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAN60309.1| hypothetical protein VITISV_015004 [Vitis vinifera] 501 e-139
ref|XP_002283502.1| PREDICTED: isoflavone 2'-hydroxylase-like [V... 501 e-139
ref|XP_003632506.1| PREDICTED: LOW QUALITY PROTEIN: isoflavone 2... 496 e-138
ref|XP_002279038.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis ... 496 e-138
emb|CAN72520.1| hypothetical protein VITISV_040350 [Vitis vinifera] 495 e-138
>emb|CAN60309.1| hypothetical protein VITISV_015004 [Vitis vinifera]
Length = 990
Score = 501 bits (1291), Expect = e-139
Identities = 245/428 (57%), Positives = 320/428 (74%), Gaps = 8/428 (1%)
Frame = +1
Query: 67 LPPSPLTKLPIIGHLHLIRSPIHRTLETFSKTIGPIFSLNFGSRLXXXXXXXXXXXXCFT 246
LPPSP PI GHLHL++ P+HRTL S+ GPI SL FGSR CFT
Sbjct: 515 LPPSP-PGFPIXGHLHLLKGPLHRTLHRLSERHGPIVSLRFGSRPVIVVSSPSAVEECFT 573
Query: 247 INDVVLANRPRHLIGKYIGYNWSIMVTASYGDHWRNLRRVSTLEIFSTTRLNSFVSIRQD 426
NDV+ ANRP+ ++GKYIGY+++++ A YGDHWRNLRR+S +EIF++ RLN F+ IR+D
Sbjct: 574 KNDVIFANRPKFVMGKYIGYDYTVVSLAPYGDHWRNLRRLSAVEIFASNRLNLFLGIRRD 633
Query: 427 EVAQLLLSISR---KGNGEVVLKHKLNELSFNIIMRMIAGKRYYGDVVDDEEDAKHVLGI 597
E+ QLLL +SR + +V LK +EL NI MRM+AGKR+YGD + D E+A+ I
Sbjct: 634 EIKQLLLRLSRNSVENFAKVELKSMFSELLLNITMRMVAGKRFYGDNMKDVEEAREFREI 693
Query: 598 MEEVLSMAAASYPGDFIPFLRWIDYKNYEKRLRVLSKKLDKVLERIIEEHRCD-----ES 762
+E+L S PGDF P L+WIDY+ Y KR L KK+D L+ +++E R + E+
Sbjct: 694 SKEILEFXGTSNPGDFXPILQWIDYQGYNKRALRLGKKMDVFLQGLLDECRSNKRSDLEN 753
Query: 763 RNSMVAHLLSLQESQPDYYSDVTIKGLMVVLIFAGSDTSTVTLEWAMSLLLNHPQVLKRA 942
RN+M+ HLLSLQES+P+YY+D IKGL+V + G+DT+ VT+EWAMSLLLNHP+VLK+A
Sbjct: 754 RNTMIDHLLSLQESEPEYYTDEIIKGLIVAMQVGGADTTAVTIEWAMSLLLNHPEVLKKA 813
Query: 943 REEIDTVIGQDRLVVESDLSKLNYLQNIIWETLRLFPATPLLLPHQASADCKIGGYDIPA 1122
R+E+DT IG D L+ E+DL KL YLQ+II E+LRLFP+TPLL+PH ++ DCK+GG+D+P
Sbjct: 814 RDELDTHIGHDCLIDETDLPKLQYLQSIISESLRLFPSTPLLVPHFSTEDCKLGGFDVPG 873
Query: 1123 GTLLLVNAWSIHRDPKVWDDPTSFKPERFEGIEIETHKLMPFGMGRRSCPGYGLAQRVLG 1302
GT+LLVNAW++HRDPK+W+DPTSFKPERFE E ET+KL+PFG+GRR+CPG GLA RV+G
Sbjct: 874 GTMLLVNAWALHRDPKLWNDPTSFKPERFETGESETYKLLPFGVGRRACPGIGLANRVMG 933
Query: 1303 LVLASLIQ 1326
L L SLIQ
Sbjct: 934 LTLGSLIQ 941
Score = 493 bits (1268), Expect = e-137
Identities = 243/434 (55%), Positives = 322/434 (74%), Gaps = 9/434 (2%)
Frame = +1
Query: 52 RSSKQLPPSPLTKLPIIGHLHLIRSPIHRTLETFSKTIGPIFSLNFGSRLXXXXXXXXXX 231
R LPPSP +PI+GHLHL++ P HR L S+T GPIFSL FGS+L
Sbjct: 27 RGHXNLPPSP-PAVPILGHLHLLKGPFHRALHHLSETYGPIFSLRFGSQLVVVISSSSAV 85
Query: 232 XXCFTINDVVLANRPRHLIGKYIGYNWSIMVTASYGDHWRNLRRVSTLEIFSTTRLNSFV 411
CFT NDV+ ANRPR ++ +Y+GY ++ +V++ YG+HWRNLRR+ LEIFS+ RLN F+
Sbjct: 86 EECFTKNDVIFANRPRLMVSEYLGYKYTSIVSSPYGEHWRNLRRLCALEIFSSNRLNMFL 145
Query: 412 SIRQDEVAQLLLSI---SRKGNGEVVLKHKLNELSFNIIMRMIAGKRYYGDVVDDEEDAK 582
IR+DE+ LL + SR +V LK +EL+FNII RM+AGKRYYG+ D EE AK
Sbjct: 146 GIRKDEIKHLLRRLGGDSRDNFAKVELKSLFSELTFNIITRMVAGKRYYGEGSDFEE-AK 204
Query: 583 HVLGIMEEVLSMAAASYPGDFIPFLRWIDYKNYEKRLRVLSKKLDKVLERIIEEHRCDES 762
H I+ + ++AAS PGDF+P LRW+DY YEK++ S++LD +L+ +I+EHR +
Sbjct: 205 HFREIIRKSFLLSAASNPGDFLPILRWMDYGGYEKKMAKNSRELDVILQGLIDEHRSNSK 264
Query: 763 R-----NSMVAHLLSLQESQPDYYSDVTIKGLMVVLIFAGSDTSTVTLEWAMSLLLNHPQ 927
+ N+M+ HLLSLQ+S+P+YY+D IKG+ + L+FAG+DT+ VT+EWAMSLLLNHP
Sbjct: 265 KGLMGNNTMIDHLLSLQKSEPEYYTDQIIKGVTMNLVFAGTDTAAVTMEWAMSLLLNHPD 324
Query: 928 VLKRAREEIDTVIGQDRLVVESDLSKLNYLQNIIWETLRLFPATPLLLPHQASADCKIGG 1107
VLK+A+ E+DT +GQ+RL+ E+DL KL+YLQNII ET RL P PL LPH +SA+C++GG
Sbjct: 325 VLKKAKVELDTXVGQERLLEEADLPKLHYLQNIISETFRLCPPAPLWLPHMSSANCQLGG 384
Query: 1108 YDIPAGTLLLVNAWSIHRDPKVWDDPTSFKPERFEGIEI-ETHKLMPFGMGRRSCPGYGL 1284
+DIP +LLVN+W++HRDPK+WDDPTSFKPERFEG E ET+KL+PFG GRR+CPG GL
Sbjct: 385 FDIPRDXMLLVNSWTLHRDPKLWDDPTSFKPERFEGGERGETYKLLPFGTGRRACPGSGL 444
Query: 1285 AQRVLGLVLASLIQ 1326
A +V+GL L SLIQ
Sbjct: 445 ANKVVGLTLGSLIQ 458
>ref|XP_002283502.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
Length = 508
Score = 501 bits (1289), Expect = e-139
Identities = 244/428 (57%), Positives = 321/428 (75%), Gaps = 8/428 (1%)
Frame = +1
Query: 67 LPPSPLTKLPIIGHLHLIRSPIHRTLETFSKTIGPIFSLNFGSRLXXXXXXXXXXXXCFT 246
LPPSP PI GHLHL++ P+HRTL S+ GPI SL FGSR CFT
Sbjct: 33 LPPSP-PGFPIFGHLHLLKGPLHRTLHRLSERHGPIVSLRFGSRPVIVVSSPSAVEECFT 91
Query: 247 INDVVLANRPRHLIGKYIGYNWSIMVTASYGDHWRNLRRVSTLEIFSTTRLNSFVSIRQD 426
NDV+ ANRP+ ++GKYIGY+++++ A YGDHWRNLRR+S +EIF++ RLN F+ IR+D
Sbjct: 92 KNDVIFANRPKFVMGKYIGYDYTVVSLAPYGDHWRNLRRLSAVEIFASNRLNLFLGIRRD 151
Query: 427 EVAQLLLSISR---KGNGEVVLKHKLNELSFNIIMRMIAGKRYYGDVVDDEEDAKHVLGI 597
E+ QLLL +SR + +V LK +EL NI MRM+AGKR+YGD + D E+A+ I
Sbjct: 152 EIKQLLLRLSRNSVENFAKVELKSMFSELLLNITMRMVAGKRFYGDNMKDVEEAREFREI 211
Query: 598 MEEVLSMAAASYPGDFIPFLRWIDYKNYEKRLRVLSKKLDKVLERIIEEHRCD-----ES 762
+E+L + S PGDF+P L+WIDY+ Y KR L KK+D L+ +++E R + E+
Sbjct: 212 SKEILEFSGTSNPGDFLPILQWIDYQGYNKRALRLGKKMDVFLQGLLDECRSNKRSDLEN 271
Query: 763 RNSMVAHLLSLQESQPDYYSDVTIKGLMVVLIFAGSDTSTVTLEWAMSLLLNHPQVLKRA 942
RN+M+ HLLSLQES+P+YY+D IKGL+V + G+DT+ VT+EWAMSLLLNHP+VLK+A
Sbjct: 272 RNTMIDHLLSLQESEPEYYTDEIIKGLIVAMQVGGADTTAVTIEWAMSLLLNHPEVLKKA 331
Query: 943 REEIDTVIGQDRLVVESDLSKLNYLQNIIWETLRLFPATPLLLPHQASADCKIGGYDIPA 1122
R+E+DT IG D L+ E+DL KL YLQ+II E+LRLFP+TPLL+PH ++ DCK+ G+D+P
Sbjct: 332 RDELDTHIGHDCLIDETDLPKLQYLQSIISESLRLFPSTPLLVPHFSTEDCKLRGFDVPG 391
Query: 1123 GTLLLVNAWSIHRDPKVWDDPTSFKPERFEGIEIETHKLMPFGMGRRSCPGYGLAQRVLG 1302
GT+LLVNAW++HRDPK+W+DPTSFKPERFE E ET+KL+PFG+GRR+CPG GLA RV+G
Sbjct: 392 GTMLLVNAWALHRDPKLWNDPTSFKPERFETGESETYKLLPFGVGRRACPGIGLANRVMG 451
Query: 1303 LVLASLIQ 1326
L L SLIQ
Sbjct: 452 LTLGSLIQ 459
>ref|XP_003632506.1| PREDICTED: LOW QUALITY PROTEIN: isoflavone 2'-hydroxylase-like [Vitis
vinifera]
Length = 498
Score = 496 bits (1277), Expect = e-138
Identities = 249/433 (57%), Positives = 318/433 (73%), Gaps = 8/433 (1%)
Frame = +1
Query: 52 RSSKQLPPSPLTKLPIIGHLHLI-RSPIHRTLETFSKTIGPIFSLNFGSRLXXXXXXXXX 228
R LPPSP +PI+GHLHL+ + PIHR L+ SK GPIFSL FGS
Sbjct: 27 RIHPHLPPSP-PAIPILGHLHLLLKPPIHRQLQNLSKKYGPIFSLRFGSSPVVIISSPST 85
Query: 229 XXXCFTINDVVLANRPRHLIGKYIGYNWSIMVTASYGDHWRNLRRVSTLEIFSTTRLNSF 408
CFT ND++ ANRPR LIGKYIGYN++ + +ASY +HWRNL R+S LEIFS+ RLN F
Sbjct: 86 VEECFTKNDIIFANRPRLLIGKYIGYNYTTIASASYDEHWRNLHRLSALEIFSSNRLNMF 145
Query: 409 VSIRQDEVAQLLLSISRKGN---GEVVLKHKLNELSFNIIMRMIAGKRYYGDVVDDEEDA 579
+ IR+DE+ LL +S+K V L+ EL+ I+MRM+AGKRYYG+ VD EE
Sbjct: 146 LGIRRDEIKILLHRLSQKSRDNFARVELRPMFTELTCYILMRMVAGKRYYGEAVDSEE-G 204
Query: 580 KHVLGIMEEVLSMAAASYPGDFIPFLRWIDYKNYEKRLRVLSKKLDKVLERIIEEHRCDE 759
KH IM + +A AS PGDF+P LRW+D+ YEK ++ L+++ D + + +I+EHR +
Sbjct: 205 KHFQKIMRGIFELAGASNPGDFLPLLRWVDFGGYEKSVK-LNREKDVIFQGLIKEHRSPD 263
Query: 760 ----SRNSMVAHLLSLQESQPDYYSDVTIKGLMVVLIFAGSDTSTVTLEWAMSLLLNHPQ 927
++NSM+ HLLSLQ+S+P+YY+D IKGL ++LIFAG+DT+ T+EWAMSLLLNHP
Sbjct: 264 QGLVNKNSMIDHLLSLQKSEPEYYTDEIIKGLALILIFAGTDTTATTIEWAMSLLLNHPD 323
Query: 928 VLKRAREEIDTVIGQDRLVVESDLSKLNYLQNIIWETLRLFPATPLLLPHQASADCKIGG 1107
VLK+AR E+DT +G+DRL ESD KL YL++II ETLRLFPATPLL+PH +S +C+IGG
Sbjct: 324 VLKKARAELDTHVGKDRLTEESDFPKLQYLRSIISETLRLFPATPLLMPHISSDNCQIGG 383
Query: 1108 YDIPAGTLLLVNAWSIHRDPKVWDDPTSFKPERFEGIEIETHKLMPFGMGRRSCPGYGLA 1287
+DIP GT+LLVNAW+IHRDPK W DPTSFKPERFE E E +KL+PFG+GRR+CPG GLA
Sbjct: 384 FDIPRGTILLVNAWAIHRDPKSWKDPTSFKPERFENEEGEAYKLLPFGLGRRACPGAGLA 443
Query: 1288 QRVLGLVLASLIQ 1326
RV+GL L LIQ
Sbjct: 444 NRVIGLTLGLLIQ 456
>ref|XP_002279038.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis vinifera]
Length = 502
Score = 496 bits (1277), Expect = e-138
Identities = 246/429 (57%), Positives = 324/429 (75%), Gaps = 9/429 (2%)
Frame = +1
Query: 67 LPPSPLTKLPIIGHLHLIRSPIHRTLETFSKTIGPIFSLNFGSRLXXXXXXXXXXXXCFT 246
LPPSP LPIIGHLHL++ P+HR+L+ S+ GPIFSL FGS+L CFT
Sbjct: 34 LPPSP-PALPIIGHLHLLKQPVHRSLQRLSQKYGPIFSLRFGSQLAVIVSSPSAVEECFT 92
Query: 247 INDVVLANRPRHLIGKYIGYNWSIMVTASYGDHWRNLRRVSTLEIFSTTRLNSFVSIRQD 426
NDVVLA+RPR GKY+G+N++ + ASYG+HWRNLRRVS LEIFS+ RLN F+ IR+D
Sbjct: 93 KNDVVLADRPRLASGKYVGFNYTTITAASYGEHWRNLRRVSALEIFSSNRLNMFLGIRRD 152
Query: 427 EVAQLLLSI---SRKGNGEVVLKHKLNELSFNIIMRMIAGKRYYGDVVDDEEDAKHVLGI 597
EV +LLL + SR+G +V ++ L EL+FNII RM+AGKRYYG+ ++ E AK I
Sbjct: 153 EVKRLLLRLARDSREGFAKVEMRPMLTELNFNIITRMVAGKRYYGEDLEYAE-AKRFRDI 211
Query: 598 MEEVLSMAAA-SYPGDFIPFLRWIDYKNYEKRLRVLSKKLDKVLERIIEEHRCDESR--- 765
+ E+ + A S P DF+P LRWI + N+EK+L+ ++++ +L+ +I+EHR +
Sbjct: 212 ISEIFELLGALSNPADFLPILRWIGFGNHEKKLKKITRETKAILQGLIDEHRSGNDKGSV 271
Query: 766 --NSMVAHLLSLQESQPDYYSDVTIKGLMVVLIFAGSDTSTVTLEWAMSLLLNHPQVLKR 939
NSM+ HLLSLQ+++P+YY+D IKGL++VLI G+DTS T+EWAM+LLLNHP VL++
Sbjct: 272 DNNSMIDHLLSLQKTEPEYYTDDIIKGLVLVLILGGTDTSAATMEWAMTLLLNHPDVLEK 331
Query: 940 AREEIDTVIGQDRLVVESDLSKLNYLQNIIWETLRLFPATPLLLPHQASADCKIGGYDIP 1119
A+ E+D +G+DRL+ ESDL KL YLQ+II ET+R FP PLL+PH +S DC+IGG+DIP
Sbjct: 332 AKVELDMHVGKDRLIEESDLPKLRYLQSIISETMRAFPVGPLLVPHMSSDDCQIGGFDIP 391
Query: 1120 AGTLLLVNAWSIHRDPKVWDDPTSFKPERFEGIEIETHKLMPFGMGRRSCPGYGLAQRVL 1299
GTLLLVNAW++HRDP+VW+DPTSFKPERFE E E +KL+PFG+GRR+CPG GLAQRV+
Sbjct: 392 RGTLLLVNAWALHRDPQVWEDPTSFKPERFENGEREDYKLVPFGIGRRACPGAGLAQRVV 451
Query: 1300 GLVLASLIQ 1326
GL L SLIQ
Sbjct: 452 GLALGSLIQ 460
>emb|CAN72520.1| hypothetical protein VITISV_040350 [Vitis vinifera]
Length = 502
Score = 495 bits (1275), Expect = e-138
Identities = 246/429 (57%), Positives = 323/429 (75%), Gaps = 9/429 (2%)
Frame = +1
Query: 67 LPPSPLTKLPIIGHLHLIRSPIHRTLETFSKTIGPIFSLNFGSRLXXXXXXXXXXXXCFT 246
LPPSP LPIIGHLHL+ P+HR+L+ S+ GPIFSL FGS+L CFT
Sbjct: 34 LPPSP-PALPIIGHLHLLXQPVHRSLQRLSQKYGPIFSLRFGSQLAVIVSSPSAVEECFT 92
Query: 247 INDVVLANRPRHLIGKYIGYNWSIMVTASYGDHWRNLRRVSTLEIFSTTRLNSFVSIRQD 426
NDVVLA+RPR GKY+G+N++ + ASYG+HWRNLRRVS LEIFS+ RLN F+ IR+D
Sbjct: 93 KNDVVLADRPRLAXGKYVGFNYTTITAASYGEHWRNLRRVSALEIFSSNRLNMFLGIRRD 152
Query: 427 EVAQLLLSI---SRKGNGEVVLKHKLNELSFNIIMRMIAGKRYYGDVVDDEEDAKHVLGI 597
EV +LLL + SR+G +V ++ L EL+FNII RM+AGKRYYG+ ++ E AK I
Sbjct: 153 EVKRLLLRLARDSREGFAKVEMRPMLTELNFNIITRMVAGKRYYGEDLEYAE-AKRFRDI 211
Query: 598 MEEVLSMAAA-SYPGDFIPFLRWIDYKNYEKRLRVLSKKLDKVLERIIEEHRCDESR--- 765
+ E+ + A S P DF+P LRWI + N+EK+L+ ++++ +L+ +I+EHR +
Sbjct: 212 ISEIFELLGALSNPADFLPILRWIGFGNHEKKLKKITRETKAILQGLIDEHRSGNDKGSV 271
Query: 766 --NSMVAHLLSLQESQPDYYSDVTIKGLMVVLIFAGSDTSTVTLEWAMSLLLNHPQVLKR 939
NSM+ HLLSLQ+++P+YY+D IKGL++VLI G+DTS T+EWAM+LLLNHP VL++
Sbjct: 272 DNNSMIDHLLSLQKTEPEYYTDDIIKGLVLVLILGGTDTSAATMEWAMTLLLNHPDVLEK 331
Query: 940 AREEIDTVIGQDRLVVESDLSKLNYLQNIIWETLRLFPATPLLLPHQASADCKIGGYDIP 1119
A+ E+D +G+DRL+ ESDL KL YLQ+II ET+R FP PLL+PH +S DC+IGG+DIP
Sbjct: 332 AKVELDMHVGKDRLIEESDLPKLRYLQSIISETMRAFPVGPLLVPHMSSDDCQIGGFDIP 391
Query: 1120 AGTLLLVNAWSIHRDPKVWDDPTSFKPERFEGIEIETHKLMPFGMGRRSCPGYGLAQRVL 1299
GTLLLVNAW++HRDP+VW+DPTSFKPERFE E E +KL+PFG+GRR+CPG GLAQRV+
Sbjct: 392 RGTLLLVNAWALHRDPQVWEDPTSFKPERFENGEREDYKLVPFGIGRRACPGAGLAQRVV 451
Query: 1300 GLVLASLIQ 1326
GL L SLIQ
Sbjct: 452 GLALGSLIQ 460