BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00021230
         (468 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002889456.1| C2 domain-containing protein [Arabidopsis ly...   107   8e-22
ref|NP_171836.3| C2 calcium/lipid-binding and GRAM domain contai...   105   5e-21
ref|XP_002329933.1| predicted protein [Populus trichocarpa] gi|2...    99   3e-19
ref|XP_002308750.1| predicted protein [Populus trichocarpa] gi|2...    97   1e-18
ref|XP_004159121.1| PREDICTED: C2 and GRAM domain-containing pro...    93   2e-17

>ref|XP_002889456.1| C2 domain-containing protein [Arabidopsis lyrata subsp. lyrata]
            gi|297335298|gb|EFH65715.1| C2 domain-containing protein
            [Arabidopsis lyrata subsp. lyrata]
          Length = 1872

 Score =  107 bits (268), Expect = 8e-22
 Identities = 50/71 (70%), Positives = 61/71 (85%)
 Frame = +2

Query: 254  INSVMELFGGNELEQKAMETAGCLSYSHSPWESEKADVYERQVYYRSKQISQYKGEVTST 433
            ++  MELFGG E+++KAME AGC SYS SPWESEKADVYERQ YYR K+IS+Y+GEVTST
Sbjct: 1710 VSFFMELFGGGEMDRKAMERAGCQSYSCSPWESEKADVYERQTYYRDKRISRYRGEVTST 1769

Query: 434  QQKSRLCDKDG 466
            QQKS + +K+G
Sbjct: 1770 QQKSLVPEKNG 1780


>ref|NP_171836.3| C2 calcium/lipid-binding and GRAM domain containing protein
            [Arabidopsis thaliana]
            gi|75315948|sp|Q9ZVT9.4|C2GR1_ARATH RecName: Full=C2 and
            GRAM domain-containing protein At1g03370
            gi|15778696|gb|AAC72128.2| Contains similarity to
            gb|AB011110 KIAA0538 protein from Homo sapiens brain and
            to phospholipid-binding domain C2 PF|00168. ESTs
            gb|AA585988 and gb|T04384 come from this gene
            [Arabidopsis thaliana] gi|21539553|gb|AAM53329.1| unknown
            protein [Arabidopsis thaliana]
            gi|332189444|gb|AEE27565.1| C2 calcium/lipid-binding and
            GRAM domain containing protein [Arabidopsis thaliana]
          Length = 1020

 Score =  105 bits (261), Expect = 5e-21
 Identities = 49/71 (69%), Positives = 60/71 (84%)
 Frame = +2

Query: 254  INSVMELFGGNELEQKAMETAGCLSYSHSPWESEKADVYERQVYYRSKQISQYKGEVTST 433
            ++  MELFGG E+++KAME AGC SYS SPWESEK DVYERQ YYR K+IS+Y+GEVTST
Sbjct: 861  VSFFMELFGGGEVDRKAMERAGCQSYSCSPWESEKDDVYERQTYYRDKRISRYRGEVTST 920

Query: 434  QQKSRLCDKDG 466
            QQKS + +K+G
Sbjct: 921  QQKSLVPEKNG 931


>ref|XP_002329933.1| predicted protein [Populus trichocarpa] gi|222871955|gb|EEF09086.1|
            predicted protein [Populus trichocarpa]
          Length = 1020

 Score = 99.4 bits (246), Expect = 3e-19
 Identities = 54/102 (52%), Positives = 69/102 (67%), Gaps = 3/102 (2%)
 Frame = +2

Query: 170  SSSFRGVHIIGMYANTILTTNVSGELFQI--NSVMELFGGNELEQKAMETAGCLSYSHSP 343
            S SF G+  + M       + V    F +  N VME+FGG EL++K ME AGCLSYS++P
Sbjct: 844  SGSFLGLEDVSM-------SEVYAASFSVPTNFVMEMFGGGELDRKVMEKAGCLSYSYTP 896

Query: 344  WESEKADVYERQVYYR-SKQISQYKGEVTSTQQKSRLCDKDG 466
            WES K DV+ERQ+YYR  K+IS++ GEVTSTQQK  L D+ G
Sbjct: 897  WESVKTDVHERQIYYRFDKRISRFGGEVTSTQQKYPLSDRKG 938


>ref|XP_002308750.1| predicted protein [Populus trichocarpa] gi|222854726|gb|EEE92273.1|
            predicted protein [Populus trichocarpa]
          Length = 1012

 Score = 97.1 bits (240), Expect = 1e-18
 Identities = 46/73 (63%), Positives = 58/73 (79%), Gaps = 1/73 (1%)
 Frame = +2

Query: 251  QINSVMELFGGNELEQKAMETAGCLSYSHSPWESEKADVYERQVYYR-SKQISQYKGEVT 427
            +IN + ELFGG EL++K ME AGCLSYS++PWES K +VYERQ+YYR  K +S++ GEVT
Sbjct: 858  EINFLSELFGGGELDRKVMEKAGCLSYSYTPWESVKTEVYERQLYYRFDKHVSRFGGEVT 917

Query: 428  STQQKSRLCDKDG 466
            STQQK  L D+ G
Sbjct: 918  STQQKYPLSDRKG 930


>ref|XP_004159121.1| PREDICTED: C2 and GRAM domain-containing protein At1g03370-like
           [Cucumis sativus]
          Length = 210

 Score = 93.2 bits (230), Expect = 2e-17
 Identities = 55/111 (49%), Positives = 71/111 (63%), Gaps = 1/111 (0%)
 Frame = +2

Query: 137 SHAPYDLCVHASSSFRGVHIIGMYANTILTTNVSGELFQINSVMELFGGNELEQKAMETA 316
           S A   L    S SF G   + M  + +L+T +S      N  MELF G +LE+K ME A
Sbjct: 22  SEAKGCLQTEESGSFLGPSEVSM--SEVLSTTLS---VPTNFAMELFNGADLERKVMEKA 76

Query: 317 GCLSYSHSPWESEKADVYERQVYY-RSKQISQYKGEVTSTQQKSRLCDKDG 466
           GCL+YS +PWESEK +VYERQ+YY   K+IS Y+ EVTSTQQ+  L +K+G
Sbjct: 77  GCLNYSFTPWESEKENVYERQIYYIFDKRISHYRVEVTSTQQRHSLPNKNG 127