BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00021092
         (1056 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P48626.1|FAD3E_TOBAC RecName: Full=Omega-3 fatty acid desatur...   428   e-118
ref|NP_001233791.1| omega-3 fatty acid desaturase [Solanum lycop...   422   e-116
gb|AAN17504.1| microsomal omega-3 fatty acid desaturase [Betula ...   418   e-114
dbj|BAG70949.1| omega-3 fatty acid desaturase [Linum grandiflorum]    414   e-114
gb|AEF80000.1| fatty acid desaturase 3 [Corylus heterophylla]         416   e-114

>sp|P48626.1|FAD3E_TOBAC RecName: Full=Omega-3 fatty acid desaturase, endoplasmic reticulum
            gi|599592|dbj|BAA05515.1| microsomal omega-3 acid
            desaturase [Nicotiana tabacum]
            gi|21668484|dbj|BAC01273.1| microsomal omega-3 fatty acid
            desaturase [Nicotiana tabacum]
          Length = 379

 Score =  428 bits (1100), Expect(2) = e-118
 Identities = 191/265 (72%), Positives = 215/265 (81%)
 Frame = -3

Query: 1054 VLGHDCGHGSFSDSPRLNSIMGHVLHSSILVPYHGWRISHRTHHQNHGNIEKDESWVPMP 875
            VLGHDCGHGSFSDS  LN+++GH+LHS+ILVPYHGWRISH+THHQNHGN+E DESWVPMP
Sbjct: 94   VLGHDCGHGSFSDSQLLNNVVGHILHSAILVPYHGWRISHKTHHQNHGNVETDESWVPMP 153

Query: 874  EKIYKSLPVHIKLLRFKIPFPMFAYPLYLISRSPGKKGSHFNPCSDLFHPSERTLVMTST 695
            EK+Y  +    K LR+KIPFP+ AYP+YL+ RSPGK GSHFNP SDLF P ER  V+TST
Sbjct: 154  EKLYNKVGYSTKFLRYKIPFPLLAYPMYLMKRSPGKSGSHFNPYSDLFQPHERKYVVTST 213

Query: 694  FCWTATLAFLGYIASAIGFVQMLKLYGVPYMIFVMWIDCVTYLHHHGYDKKLPWYRGKEW 515
             CWT   A L Y+ +A G +QM K+YG PY+IFVMW+D VTYLHHHGY+KKLPWYRGKEW
Sbjct: 214  LCWTVMAALLLYLCTAFGSLQMFKIYGAPYLIFVMWLDFVTYLHHHGYEKKLPWYRGKEW 273

Query: 514  SYLRGGLTTIDRDYGLINNXXXXXXXXXXXXLFPQIPHYHLREATAAIKPVLGDYYREPR 335
            SYLRGGLTT+DRDYGL NN            LFPQIPHYHLREAT A KPVLG YYREP+
Sbjct: 274  SYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLREATKAAKPVLGKYYREPK 333

Query: 334  KSGLFPFHLTKDLLRSIKQDHYVSD 260
            KSG  PFHL KDL RS+KQDHYVSD
Sbjct: 334  KSGPIPFHLVKDLTRSMKQDHYVSD 358



 Score = 23.5 bits (49), Expect(2) = e-118
 Identities = 8/12 (66%), Positives = 10/12 (83%)
 Frame = -2

Query: 164 GEVVFYQKDPQM 129
           GE+VFYQ DP +
Sbjct: 360 GEIVFYQTDPHI 371


>ref|NP_001233791.1| omega-3 fatty acid desaturase [Solanum lycopersicum]
            gi|160334210|gb|ABX24525.1| omega-3 fatty acid desaturase
            [Solanum lycopersicum]
          Length = 377

 Score =  422 bits (1084), Expect = e-116
 Identities = 187/265 (70%), Positives = 219/265 (82%)
 Frame = -3

Query: 1054 VLGHDCGHGSFSDSPRLNSIMGHVLHSSILVPYHGWRISHRTHHQNHGNIEKDESWVPMP 875
            VLGHDCGHGSFSDS  LNS++GH+LHS ILVPYHGWRISH+TH QNHGN+E DESWVPMP
Sbjct: 91   VLGHDCGHGSFSDSALLNSVVGHILHSFILVPYHGWRISHKTHRQNHGNVEADESWVPMP 150

Query: 874  EKIYKSLPVHIKLLRFKIPFPMFAYPLYLISRSPGKKGSHFNPCSDLFHPSERTLVMTST 695
            EK+YK L    KLLRFKIPFP+ AYPLYL+ RSPGKKGSHFNP SDLF  +ER  ++TST
Sbjct: 151  EKLYKELDYATKLLRFKIPFPLIAYPLYLMRRSPGKKGSHFNPYSDLFQENERKFIVTST 210

Query: 694  FCWTATLAFLGYIASAIGFVQMLKLYGVPYMIFVMWIDCVTYLHHHGYDKKLPWYRGKEW 515
             CWT  +A L Y+++ +GF+Q+LKLYG+PYMIFVMW+D VTYLHHHG+++KLPWYRGKEW
Sbjct: 211  LCWTLMVALLFYLSTVVGFLQLLKLYGIPYMIFVMWLDSVTYLHHHGHEEKLPWYRGKEW 270

Query: 514  SYLRGGLTTIDRDYGLINNXXXXXXXXXXXXLFPQIPHYHLREATAAIKPVLGDYYREPR 335
            SYLRGGLTT+DR YG+ NN            LFPQIPHYHL EAT A KPV G YYREP+
Sbjct: 271  SYLRGGLTTVDRGYGVFNNIHHDIDTHVIHHLFPQIPHYHLVEATKAAKPVFGKYYREPK 330

Query: 334  KSGLFPFHLTKDLLRSIKQDHYVSD 260
            KSG  PFHL ++L++S+K+DHYVSD
Sbjct: 331  KSGAIPFHLVENLVKSMKKDHYVSD 355


>gb|AAN17504.1| microsomal omega-3 fatty acid desaturase [Betula pendula]
          Length = 386

 Score =  418 bits (1075), Expect = e-114
 Identities = 189/265 (71%), Positives = 220/265 (83%)
 Frame = -3

Query: 1054 VLGHDCGHGSFSDSPRLNSIMGHVLHSSILVPYHGWRISHRTHHQNHGNIEKDESWVPMP 875
            VLGHDCGHGSFSD+P LN+++GHVLHS+ILVPYHGWRISHRTHHQNHGN+E DESWVP+ 
Sbjct: 100  VLGHDCGHGSFSDNPELNNLVGHVLHSAILVPYHGWRISHRTHHQNHGNVENDESWVPLT 159

Query: 874  EKIYKSLPVHIKLLRFKIPFPMFAYPLYLISRSPGKKGSHFNPCSDLFHPSERTLVMTST 695
            EK+YKSL    +LLRF +PFP+FAYP+YL SRSPGK+GSHFNP S+LF P+ER  V+TST
Sbjct: 160  EKLYKSLGYSTRLLRFTVPFPLFAYPIYLWSRSPGKEGSHFNPYSNLFSPNERKDVITST 219

Query: 694  FCWTATLAFLGYIASAIGFVQMLKLYGVPYMIFVMWIDCVTYLHHHGYDKKLPWYRGKEW 515
             CW+   A L Y + AIG +QMLKLYGVP++IFVMW+D VTYLHHHGY++KLPWYRGKEW
Sbjct: 220  LCWSLMAALLIYSSCAIGPIQMLKLYGVPHLIFVMWLDLVTYLHHHGYEQKLPWYRGKEW 279

Query: 514  SYLRGGLTTIDRDYGLINNXXXXXXXXXXXXLFPQIPHYHLREATAAIKPVLGDYYREPR 335
            SYLRGGLTT+DRDYG  NN            LFPQIPHYHL EAT A KPVLG YYREP+
Sbjct: 280  SYLRGGLTTVDRDYGWFNNIHHDIGTHVIHHLFPQIPHYHLVEATNAAKPVLGKYYREPK 339

Query: 334  KSGLFPFHLTKDLLRSIKQDHYVSD 260
            +SG FP HL K+L+RSI +DHYV+D
Sbjct: 340  RSGPFPIHLIKNLVRSISEDHYVND 364


>dbj|BAG70949.1| omega-3 fatty acid desaturase [Linum grandiflorum]
          Length = 390

 Score =  414 bits (1063), Expect(2) = e-114
 Identities = 186/265 (70%), Positives = 214/265 (80%)
 Frame = -3

Query: 1054 VLGHDCGHGSFSDSPRLNSIMGHVLHSSILVPYHGWRISHRTHHQNHGNIEKDESWVPMP 875
            VLGHDCGHGSFSDS  LN++MGH+LHSSILVPYHGWRISH+THHQNHGN+EKDESWVP+P
Sbjct: 100  VLGHDCGHGSFSDSWLLNNVMGHILHSSILVPYHGWRISHKTHHQNHGNVEKDESWVPLP 159

Query: 874  EKIYKSLPVHIKLLRFKIPFPMFAYPLYLISRSPGKKGSHFNPCSDLFHPSERTLVMTST 695
            EK+YKSL    K  RF +P PMFAYP+YL +RSPGKKGSHFNP SDLF P+ER  V  ST
Sbjct: 160  EKLYKSLDTSTKFFRFTVPLPMFAYPIYLWTRSPGKKGSHFNPYSDLFAPNERAAVTIST 219

Query: 694  FCWTATLAFLGYIASAIGFVQMLKLYGVPYMIFVMWIDCVTYLHHHGYDKKLPWYRGKEW 515
             CWTA    L Y +   GF+ +LK+YGVPY++FV W+D VTYLHHHG+++KLPWYRGKEW
Sbjct: 220  LCWTAMALLLCYASFIYGFLPVLKIYGVPYLVFVAWLDMVTYLHHHGHEQKLPWYRGKEW 279

Query: 514  SYLRGGLTTIDRDYGLINNXXXXXXXXXXXXLFPQIPHYHLREATAAIKPVLGDYYREPR 335
            SYLRGGLTT+DRDYG+INN            LFPQIPHYHL EAT A K VLG YYREP+
Sbjct: 280  SYLRGGLTTVDRDYGIINNIHHDIGTHVIHHLFPQIPHYHLVEATEAAKHVLGKYYREPK 339

Query: 334  KSGLFPFHLTKDLLRSIKQDHYVSD 260
            KSG FPFHL  +L+RS+ +DHYVSD
Sbjct: 340  KSGAFPFHLFGNLVRSLGEDHYVSD 364



 Score = 25.8 bits (55), Expect(2) = e-114
 Identities = 10/15 (66%), Positives = 12/15 (80%)
 Frame = -2

Query: 164 GEVVFYQKDPQMNVF 120
           G+VVFYQ DPQ+  F
Sbjct: 366 GDVVFYQSDPQIPKF 380


>gb|AEF80000.1| fatty acid desaturase 3 [Corylus heterophylla]
          Length = 386

 Score =  416 bits (1069), Expect = e-114
 Identities = 186/265 (70%), Positives = 219/265 (82%)
 Frame = -3

Query: 1054 VLGHDCGHGSFSDSPRLNSIMGHVLHSSILVPYHGWRISHRTHHQNHGNIEKDESWVPMP 875
            VLGHDCGHGSFSD+P LN+++GH+LHS+ILVPYHGWRISHRTHHQNHGN+E DESWVP+ 
Sbjct: 100  VLGHDCGHGSFSDNPELNNVVGHILHSAILVPYHGWRISHRTHHQNHGNVENDESWVPLT 159

Query: 874  EKIYKSLPVHIKLLRFKIPFPMFAYPLYLISRSPGKKGSHFNPCSDLFHPSERTLVMTST 695
            EK+YKSL    +LLRF +PFP+FAYP YL SRSPGK+GSHFNP S LF P+ER  V+TST
Sbjct: 160  EKLYKSLSYSTRLLRFTVPFPLFAYPFYLWSRSPGKQGSHFNPYSKLFTPNERKDVITST 219

Query: 694  FCWTATLAFLGYIASAIGFVQMLKLYGVPYMIFVMWIDCVTYLHHHGYDKKLPWYRGKEW 515
             CW+   A L  ++ AIG +QMLKLYG+P++IFVMW+D VTYLHHHGY++KLPWYRGKEW
Sbjct: 220  LCWSVMAALLISLSYAIGPIQMLKLYGIPHLIFVMWLDLVTYLHHHGYEQKLPWYRGKEW 279

Query: 514  SYLRGGLTTIDRDYGLINNXXXXXXXXXXXXLFPQIPHYHLREATAAIKPVLGDYYREPR 335
            SYLRGGLTT+DRDYG+ NN            LFPQIPHYHL EAT A KPVLG YYREP+
Sbjct: 280  SYLRGGLTTVDRDYGMFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAAKPVLGKYYREPK 339

Query: 334  KSGLFPFHLTKDLLRSIKQDHYVSD 260
            +SG FP HL K+L+RSI +DHYV+D
Sbjct: 340  RSGPFPIHLIKNLVRSIAEDHYVND 364