BLASTX nr result
ID: Bupleurum21_contig00021062
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00021062
(2202 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002521460.1| serine/threonine protein kinase, putative [R... 687 0.0
gb|ADT91697.1| serine/threonine protein kinase 2 [Nicotiana atte... 679 0.0
dbj|BAA06731.1| NPK2 [Nicotiana tabacum] 677 0.0
ref|XP_004141107.1| PREDICTED: mitogen-activated protein kinase ... 662 0.0
ref|XP_004158323.1| PREDICTED: mitogen-activated protein kinase ... 660 0.0
>ref|XP_002521460.1| serine/threonine protein kinase, putative [Ricinus communis]
gi|223539359|gb|EEF40950.1| serine/threonine protein
kinase, putative [Ricinus communis]
Length = 518
Score = 687 bits (1774), Expect(2) = 0.0
Identities = 336/392 (85%), Positives = 362/392 (92%), Gaps = 1/392 (0%)
Frame = -2
Query: 2033 MAGLEELKKKLVPLFDAEKGFPSGSTLDPSDSYMLSDGGTVNLLSRSYGVYNINELGLQK 1854
MAGLEEL+KKL PLFDAEKGF +GSTLDPSDSYMLS GGTVNLLSRSYGVYNINELGLQK
Sbjct: 1 MAGLEELRKKLTPLFDAEKGFSAGSTLDPSDSYMLSGGGTVNLLSRSYGVYNINELGLQK 60
Query: 1853 CTSRSVDDTEHGEKTYNCASHEMRVFGAIGSGASSVVQRAIHIPMHRIIALKKINIFEKE 1674
C+S VD+T+H EKTY CASHEMR+FGAIGSGASSVVQRAIHIP HRIIALKKINIFEKE
Sbjct: 61 CSSSPVDETDHSEKTYRCASHEMRIFGAIGSGASSVVQRAIHIPTHRIIALKKINIFEKE 120
Query: 1673 KRQQLLTEIRTLCEAPCYQGLVEFYGAFYTPDSGQISIALEYMDGGSLADIIRVRKQIPE 1494
KRQQLLTEIRTLCEAPC +GLVEF+GAFYTPDSGQISIALEYM+GGSLADI+RV+K+IPE
Sbjct: 121 KRQQLLTEIRTLCEAPCNEGLVEFHGAFYTPDSGQISIALEYMNGGSLADILRVQKRIPE 180
Query: 1493 AVLSLMVQKLLYGLSYLHGVRHLVHRDIKPANLLVNLKGEPKITDFGISAGLENSMAMCA 1314
VLS M QKLL+GL YLHGVR+LVHRDIKPAN+LVNLKGEPKITDFGISAGLENS+AMCA
Sbjct: 181 PVLSHMFQKLLHGLGYLHGVRYLVHRDIKPANMLVNLKGEPKITDFGISAGLENSVAMCA 240
Query: 1313 TFVGTVTYMSPERIRNENYSYPADIWSLGLALFECGTGEFPYTATEGPVNLMLQILEDPS 1134
TFVGTVTYMSPERIRNE+YSYPADIWSLGLALFECGTGEFPY A +GPVNLMLQILEDPS
Sbjct: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYAANDGPVNLMLQILEDPS 300
Query: 1133 PSPPKDIFSPEFCSFIDDCLQKDAEARPTAEQLLSHPFVRKHEVADVDLAVFVRGIFDPT 954
PSP K IFSPEFCSF+D CLQKD +ARPTA+QLLSHPF+ K+ VDLA FVR +FDP
Sbjct: 301 PSPSKQIFSPEFCSFVDACLQKDPDARPTADQLLSHPFISKYVHTSVDLAAFVRNVFDPM 360
Query: 953 QRMKDLADMLTMHYYLLFDGPDELW-HTQRPY 861
QR+KDLADMLTMHYYLLFDGPDELW HT+ Y
Sbjct: 361 QRLKDLADMLTMHYYLLFDGPDELWQHTKTLY 392
Score = 171 bits (433), Expect(2) = 0.0
Identities = 87/153 (56%), Positives = 104/153 (67%)
Frame = -1
Query: 876 HTKTLYHESSVFSFSGKESIGPNNIFKALSSIRSTLAGEWPPEKLVHIVEKLQXXXXXXX 697
HTKTLY E S FSFSGK+S+GP +IF LS+IRSTLAG+WPPE+LVH+VEKLQ
Sbjct: 387 HTKTLYKEGSTFSFSGKQSVGPKDIFANLSNIRSTLAGDWPPERLVHVVEKLQCRAHGQD 446
Query: 696 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGNQFLICGDGIQVEGVPNFKDLSIDIP 517
+GNQFLICGDG+QVEG+PN +DLSIDIP
Sbjct: 447 GVAIRVSGSFI---------------------IGNQFLICGDGLQVEGLPNIRDLSIDIP 485
Query: 516 SQRMGTFQEQFMMEAGNVIGRYVIAKQELYIIK 418
S+RMGTFQEQF+ME G V+G Y+IAKQ+LYII+
Sbjct: 486 SKRMGTFQEQFIMEPGTVVGCYIIAKQDLYIIQ 518
>gb|ADT91697.1| serine/threonine protein kinase 2 [Nicotiana attenuata]
Length = 518
Score = 679 bits (1751), Expect(2) = 0.0
Identities = 340/392 (86%), Positives = 356/392 (90%), Gaps = 1/392 (0%)
Frame = -2
Query: 2033 MAGLEELKKKLVPLFDAEKGFPSGSTLDPSDSYMLSDGGTVNLLSRSYGVYNINELGLQK 1854
MAGLEELKKKLVPLFDAEKGF ST DP DSY LSDGGTVNLLS+SYGVYNINELGLQK
Sbjct: 1 MAGLEELKKKLVPLFDAEKGFSPASTSDPFDSYSLSDGGTVNLLSQSYGVYNINELGLQK 60
Query: 1853 CTSRSVDDTEHGEKTYNCASHEMRVFGAIGSGASSVVQRAIHIPMHRIIALKKINIFEKE 1674
CTS VDD +HGEKTY CASHEMRVFGAIGSGASSVVQRAIHIP HRIIALKKINIFEKE
Sbjct: 61 CTSWPVDDADHGEKTYKCASHEMRVFGAIGSGASSVVQRAIHIPTHRIIALKKINIFEKE 120
Query: 1673 KRQQLLTEIRTLCEAPCYQGLVEFYGAFYTPDSGQISIALEYMDGGSLADIIRVRKQIPE 1494
KRQQLLTEIRTLCEAPC QGLVEFYGAFYTPDSGQISIALEYMDGGSLADII+VRK IPE
Sbjct: 121 KRQQLLTEIRTLCEAPCCQGLVEFYGAFYTPDSGQISIALEYMDGGSLADIIKVRKSIPE 180
Query: 1493 AVLSLMVQKLLYGLSYLHGVRHLVHRDIKPANLLVNLKGEPKITDFGISAGLENSMAMCA 1314
A+LS MVQKLL GLSYLHGVRHLVHRDIKPANLLVNLKGEPKITDFGISAGLE+S+AMCA
Sbjct: 181 AILSPMVQKLLNGLSYLHGVRHLVHRDIKPANLLVNLKGEPKITDFGISAGLESSIAMCA 240
Query: 1313 TFVGTVTYMSPERIRNENYSYPADIWSLGLALFECGTGEFPYTATEGPVNLMLQILEDPS 1134
TFVGTVTYMSPERIRNENYSYPADIWSLGLALFECGTGEFPYTA EGPVNLMLQIL+DPS
Sbjct: 241 TFVGTVTYMSPERIRNENYSYPADIWSLGLALFECGTGEFPYTANEGPVNLMLQILDDPS 300
Query: 1133 PSPPKDIFSPEFCSFIDDCLQKDAEARPTAEQLLSHPFVRKHEVADVDLAVFVRGIFDPT 954
PS + FSPEFCSFID CL+K+ + R TAEQLLSHPF+ K+ + VDL FVR IFDPT
Sbjct: 301 PSLSRHDFSPEFCSFIDACLKKNPDDRLTAEQLLSHPFITKYTDSAVDLGAFVRSIFDPT 360
Query: 953 QRMKDLADMLTMHYYLLFDGPDELW-HTQRPY 861
QRMKDLADMLT+HYYLLFDG DE W HT+ Y
Sbjct: 361 QRMKDLADMLTIHYYLLFDGSDEFWQHTKTLY 392
Score = 168 bits (425), Expect(2) = 0.0
Identities = 88/151 (58%), Positives = 99/151 (65%)
Frame = -1
Query: 876 HTKTLYHESSVFSFSGKESIGPNNIFKALSSIRSTLAGEWPPEKLVHIVEKLQXXXXXXX 697
HTKTLY+E S FSF KESIGP+NIF LS+IR TLAGEWPPEKLVH+VEKLQ
Sbjct: 387 HTKTLYNECSTFSFGEKESIGPSNIFSTLSNIRKTLAGEWPPEKLVHVVEKLQCRPHGQD 446
Query: 696 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGNQFLICGDGIQVEGVPNFKDLSIDIP 517
VGNQFLICGDG+QVEG+PN KDLSIDIP
Sbjct: 447 GVAIRVSGSFI---------------------VGNQFLICGDGMQVEGLPNLKDLSIDIP 485
Query: 516 SQRMGTFQEQFMMEAGNVIGRYVIAKQELYI 424
S+RMGTF EQF++E N+IGRY I KQEL+I
Sbjct: 486 SKRMGTFHEQFIVEQANIIGRYFITKQELFI 516
>dbj|BAA06731.1| NPK2 [Nicotiana tabacum]
Length = 518
Score = 677 bits (1747), Expect(2) = 0.0
Identities = 340/392 (86%), Positives = 355/392 (90%), Gaps = 1/392 (0%)
Frame = -2
Query: 2033 MAGLEELKKKLVPLFDAEKGFPSGSTLDPSDSYMLSDGGTVNLLSRSYGVYNINELGLQK 1854
MAGLEELKKKLVPLFDAEKGF ST DP DSY LSDGGTVNLLS+SYGVYNINELGLQK
Sbjct: 1 MAGLEELKKKLVPLFDAEKGFSPASTSDPFDSYSLSDGGTVNLLSQSYGVYNINELGLQK 60
Query: 1853 CTSRSVDDTEHGEKTYNCASHEMRVFGAIGSGASSVVQRAIHIPMHRIIALKKINIFEKE 1674
CTS VDD +HGEKTY CASHEMRVFGAIGSGASSVVQRAIHIP HRIIALKKINIFEKE
Sbjct: 61 CTSWPVDDADHGEKTYKCASHEMRVFGAIGSGASSVVQRAIHIPTHRIIALKKINIFEKE 120
Query: 1673 KRQQLLTEIRTLCEAPCYQGLVEFYGAFYTPDSGQISIALEYMDGGSLADIIRVRKQIPE 1494
KRQQLLTEIRTLCEAPC QGLVEFYGAFYTPDSGQISIALEYMDGGSLADII+VRK IPE
Sbjct: 121 KRQQLLTEIRTLCEAPCCQGLVEFYGAFYTPDSGQISIALEYMDGGSLADIIKVRKSIPE 180
Query: 1493 AVLSLMVQKLLYGLSYLHGVRHLVHRDIKPANLLVNLKGEPKITDFGISAGLENSMAMCA 1314
A+LS MVQKLL GLSYLHGVRHLVHRDIKPANLLVNLKGEPKITDFGISAGLE+S+AMCA
Sbjct: 181 AILSPMVQKLLNGLSYLHGVRHLVHRDIKPANLLVNLKGEPKITDFGISAGLESSIAMCA 240
Query: 1313 TFVGTVTYMSPERIRNENYSYPADIWSLGLALFECGTGEFPYTATEGPVNLMLQILEDPS 1134
TFVGTVTYMSPERIRNENYSYPADIWSLGLALFECGTGEFPYTA EGPVNLMLQIL+DPS
Sbjct: 241 TFVGTVTYMSPERIRNENYSYPADIWSLGLALFECGTGEFPYTANEGPVNLMLQILDDPS 300
Query: 1133 PSPPKDIFSPEFCSFIDDCLQKDAEARPTAEQLLSHPFVRKHEVADVDLAVFVRGIFDPT 954
PS FSPEFCSFID CL+K+ + R TAEQLLSHPF+ K+ + VDL FVR IFDPT
Sbjct: 301 PSLSGHEFSPEFCSFIDACLKKNPDDRLTAEQLLSHPFITKYTDSAVDLGAFVRSIFDPT 360
Query: 953 QRMKDLADMLTMHYYLLFDGPDELW-HTQRPY 861
QRMKDLADMLT+HYYLLFDG DE W HT+ Y
Sbjct: 361 QRMKDLADMLTIHYYLLFDGSDEFWQHTKTLY 392
Score = 169 bits (428), Expect(2) = 0.0
Identities = 87/151 (57%), Positives = 100/151 (66%)
Frame = -1
Query: 876 HTKTLYHESSVFSFSGKESIGPNNIFKALSSIRSTLAGEWPPEKLVHIVEKLQXXXXXXX 697
HTKTLY+E S FSF GKESIGP+NIF +S+IR TLAGEWPPEKLVH+VEK+Q
Sbjct: 387 HTKTLYNECSTFSFGGKESIGPSNIFSTMSNIRKTLAGEWPPEKLVHVVEKVQCRTHGQD 446
Query: 696 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGNQFLICGDGIQVEGVPNFKDLSIDIP 517
VGNQFLICGDG+QVEG+PN KDLSIDIP
Sbjct: 447 GVAIRVSGSFI---------------------VGNQFLICGDGMQVEGLPNLKDLSIDIP 485
Query: 516 SQRMGTFQEQFMMEAGNVIGRYVIAKQELYI 424
S+RMGTF EQF++E N+IGRY I KQEL+I
Sbjct: 486 SKRMGTFHEQFIVEQANIIGRYFITKQELFI 516
>ref|XP_004141107.1| PREDICTED: mitogen-activated protein kinase kinase 6-like [Cucumis
sativus]
Length = 518
Score = 662 bits (1708), Expect(2) = 0.0
Identities = 325/386 (84%), Positives = 348/386 (90%)
Frame = -2
Query: 2033 MAGLEELKKKLVPLFDAEKGFPSGSTLDPSDSYMLSDGGTVNLLSRSYGVYNINELGLQK 1854
MAGLEELKKKL PLFDAEKG S +DPSDSY SD GTVNLLSRSYGVYN NELGLQK
Sbjct: 1 MAGLEELKKKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60
Query: 1853 CTSRSVDDTEHGEKTYNCASHEMRVFGAIGSGASSVVQRAIHIPMHRIIALKKINIFEKE 1674
CTS DD+ E+TY CAS EMR+FGAIGSGASSVVQRAIHIP HRI+ALKKINIFEKE
Sbjct: 61 CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120
Query: 1673 KRQQLLTEIRTLCEAPCYQGLVEFYGAFYTPDSGQISIALEYMDGGSLADIIRVRKQIPE 1494
KRQQLLTEIRTLCEAPC +GLVEF+GAFYTPDSGQISIALEYMDGGSLAD++R++K IPE
Sbjct: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180
Query: 1493 AVLSLMVQKLLYGLSYLHGVRHLVHRDIKPANLLVNLKGEPKITDFGISAGLENSMAMCA 1314
VLS M QKLL GLSYLHGVRHLVHRDIKPANLLVNLKGE KITDFGISAGLENSMAMCA
Sbjct: 181 PVLSTMFQKLLRGLSYLHGVRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240
Query: 1313 TFVGTVTYMSPERIRNENYSYPADIWSLGLALFECGTGEFPYTATEGPVNLMLQILEDPS 1134
TFVGTVTYMSPERIRNE+YSYPADIWSLGLALFECGTGEFPY+ATEG VNLMLQIL+DPS
Sbjct: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300
Query: 1133 PSPPKDIFSPEFCSFIDDCLQKDAEARPTAEQLLSHPFVRKHEVADVDLAVFVRGIFDPT 954
PSP K FS EFCSF+D CLQKDA+ARPTAEQLLSHPF++K+E VDLA FV+ +FDPT
Sbjct: 301 PSPSKHKFSSEFCSFVDACLQKDADARPTAEQLLSHPFIKKYENEQVDLAAFVQNVFDPT 360
Query: 953 QRMKDLADMLTMHYYLLFDGPDELWH 876
QRMKDLADMLT+HYYLLFDGPD+ WH
Sbjct: 361 QRMKDLADMLTIHYYLLFDGPDDFWH 386
Score = 160 bits (405), Expect(2) = 0.0
Identities = 84/153 (54%), Positives = 101/153 (66%)
Frame = -1
Query: 876 HTKTLYHESSVFSFSGKESIGPNNIFKALSSIRSTLAGEWPPEKLVHIVEKLQXXXXXXX 697
HTK L+HESS SFSGK+ GPN+IF LS IRSTLAG+WP EKLVH+VEKLQ
Sbjct: 387 HTKALFHESSTLSFSGKQFSGPNDIFGKLSEIRSTLAGDWPLEKLVHVVEKLQCRAHGRD 446
Query: 696 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGNQFLICGDGIQVEGVPNFKDLSIDIP 517
+GNQFLICGDG+QVEG+PNFKDLSID+
Sbjct: 447 GVAIRVSGSFI---------------------LGNQFLICGDGVQVEGLPNFKDLSIDME 485
Query: 516 SQRMGTFQEQFMMEAGNVIGRYVIAKQELYIIK 418
S++MG+F+EQF++E N+IGRY IAKQELYII+
Sbjct: 486 SKKMGSFREQFIIEPSNLIGRYFIAKQELYIIQ 518
>ref|XP_004158323.1| PREDICTED: mitogen-activated protein kinase kinase 6-like [Cucumis
sativus]
Length = 518
Score = 660 bits (1702), Expect(2) = 0.0
Identities = 324/386 (83%), Positives = 347/386 (89%)
Frame = -2
Query: 2033 MAGLEELKKKLVPLFDAEKGFPSGSTLDPSDSYMLSDGGTVNLLSRSYGVYNINELGLQK 1854
MAGLEELKKKL PLFDAEKG S +DPSDSY SD GTVNLLSRSYGVYN NELGLQK
Sbjct: 1 MAGLEELKKKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60
Query: 1853 CTSRSVDDTEHGEKTYNCASHEMRVFGAIGSGASSVVQRAIHIPMHRIIALKKINIFEKE 1674
CTS DD+ E+TY CAS EMR+FGAIGSGASSVVQRAIHIP HRI+ALKKINIFEKE
Sbjct: 61 CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120
Query: 1673 KRQQLLTEIRTLCEAPCYQGLVEFYGAFYTPDSGQISIALEYMDGGSLADIIRVRKQIPE 1494
KRQQLLTEIRTLCEAPC +GLVEF+GAFYTPDSGQISIALEYMDGGSLAD++R++K IPE
Sbjct: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180
Query: 1493 AVLSLMVQKLLYGLSYLHGVRHLVHRDIKPANLLVNLKGEPKITDFGISAGLENSMAMCA 1314
VLS M QKLL GLSYLHGVRHLVHRDIKPANLLVNLKGE KITDFGISAGLENSMAMCA
Sbjct: 181 PVLSTMFQKLLRGLSYLHGVRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240
Query: 1313 TFVGTVTYMSPERIRNENYSYPADIWSLGLALFECGTGEFPYTATEGPVNLMLQILEDPS 1134
TFVGTVTYMSPERIRNE+YSYPADIWSLGLALFECGTGEFPY+ATEG VNLMLQIL+DPS
Sbjct: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300
Query: 1133 PSPPKDIFSPEFCSFIDDCLQKDAEARPTAEQLLSHPFVRKHEVADVDLAVFVRGIFDPT 954
PSP K FS EFCSF+D CLQKDA+ARPTAEQLLSHPF++K+E VDLA FV+ +FDP
Sbjct: 301 PSPSKHKFSSEFCSFVDACLQKDADARPTAEQLLSHPFIKKYENEQVDLAAFVQNVFDPI 360
Query: 953 QRMKDLADMLTMHYYLLFDGPDELWH 876
QRMKDLADMLT+HYYLLFDGPD+ WH
Sbjct: 361 QRMKDLADMLTIHYYLLFDGPDDFWH 386
Score = 162 bits (409), Expect(2) = 0.0
Identities = 85/153 (55%), Positives = 101/153 (66%)
Frame = -1
Query: 876 HTKTLYHESSVFSFSGKESIGPNNIFKALSSIRSTLAGEWPPEKLVHIVEKLQXXXXXXX 697
HTK LYHESS SFSGK+ GPN+IF LS IRSTLAG+WP EKLVH+VEKLQ
Sbjct: 387 HTKALYHESSTLSFSGKQFSGPNDIFGKLSEIRSTLAGDWPLEKLVHVVEKLQCRAHGRD 446
Query: 696 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGNQFLICGDGIQVEGVPNFKDLSIDIP 517
+GNQFLICGDG+QVEG+PNFKDLSID+
Sbjct: 447 GVAIRVSGSFI---------------------LGNQFLICGDGVQVEGLPNFKDLSIDME 485
Query: 516 SQRMGTFQEQFMMEAGNVIGRYVIAKQELYIIK 418
S++MG+F+EQF++E N+IGRY IAKQELYII+
Sbjct: 486 SKKMGSFREQFIIEPSNLIGRYFIAKQELYIIQ 518