BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00020177
         (2058 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002266990.1| PREDICTED: uncharacterized protein LOC100257...   356   2e-95
emb|CBI18661.3| unnamed protein product [Vitis vinifera]              345   3e-92
ref|XP_003532890.1| PREDICTED: uncharacterized protein LOC100782...   244   5e-62
ref|XP_003595829.1| hypothetical protein MTR_2g062300 [Medicago ...   234   5e-59
ref|NP_180751.1| uncharacterized protein [Arabidopsis thaliana] ...   192   4e-46

>ref|XP_002266990.1| PREDICTED: uncharacterized protein LOC100257965 [Vitis vinifera]
          Length = 571

 Score =  356 bits (913), Expect = 2e-95
 Identities = 199/389 (51%), Positives = 252/389 (64%), Gaps = 17/389 (4%)
 Frame = -1

Query: 1251 VPPSINQLSEKK------STLKPLNVGTSPLQNKNVIDSSSPASKPVRKETKQSAECT-- 1096
            +  S N  S+KK      S+ K  +V  SPL++KN I S   A KP++K++K S      
Sbjct: 182  ISTSSNVSSDKKASFKIDSSSKRSSVSISPLKSKNEISSPKLACKPLQKDSKSSVGVAIP 241

Query: 1095 --------SHLSKVPLSFKTWSDAKISWGSLPSRVHDLGKEVTFQRNARFLDALYALQET 940
                    S + KVP  FKTWSD  ISW SLP R+HDLGK+    RN  FL A+ AL+E 
Sbjct: 242  SRVGVAIPSRVVKVPFRFKTWSDQNISWDSLPPRIHDLGKDAASYRNVAFLAAVNALEEA 301

Query: 939  SAMENILQCMRMFAELCESSDKESSLPLVEEYLNFHQTMQRVATAVNSLLSVRFNE-EDG 763
            SA E ++QCM MFAELCESS K+S+ PLVE++LN HQ MQ+ A ++N+LLS R  E E  
Sbjct: 302  SAAEGVIQCMSMFAELCESSQKDSAGPLVEQFLNLHQDMQKAAMSINALLSTRLAEVESS 361

Query: 762  TCYSSLCSLSETCKEITDKNALSWVQAAVVTNLSKFNLFTKEDKRGDFNSEKCHYVILEN 583
            +C SS   L+E CK   +KNA+SWVQAA+ T+LSKF LF K+D R   + EKCHY++LEN
Sbjct: 362  SCCSSQPPLAEACKNFNNKNAISWVQAALETDLSKFCLFRKQDNREIRSGEKCHYIVLEN 421

Query: 582  TPIKIDVENHSPDKKRNSKKQGAVMSGSSLKTSSPYHSKQRLPXXXXXXXXXXXXXKGNG 403
            +P ++  ENHSP  K + +  G +   S+    SP  S +R               KG+G
Sbjct: 422  SP-EMKSENHSPQNKPSPRNNGGLSDSSAKGLPSP--SWRRFSASKRMSAKREEWSKGSG 478

Query: 402  LTVAANLAQKLLSTSQAWFLDYLEASLEKGFGLQKGEKDSEIACLLGQLKRVNQWLDDSV 223
            L   ANLA+KLLS S+ WFL YLE SL KGFGL++ E  +EIACLLGQLKRVNQWLD SV
Sbjct: 479  LRETANLAKKLLSVSRGWFLKYLEDSLNKGFGLKREEGGAEIACLLGQLKRVNQWLDGSV 538

Query: 222  TDQNGTNERIEGLKKKLYGFLLDNVDSAV 136
             D    +ERIEGL+KKLYGFLL++VDSAV
Sbjct: 539  EDGMEVDERIEGLRKKLYGFLLEHVDSAV 567



 Score = 98.6 bits (244), Expect = 6e-18
 Identities = 72/191 (37%), Positives = 93/191 (48%), Gaps = 15/191 (7%)
 Frame = -1

Query: 2055 GFYLRLSDSLHSAYVSVSDDDVELILTDKIQLGQFVHVARLEDGSXXXXXXXXXXXXKRR 1876
            GF+L+LSDSLHSAYVSVSD+DV+LI TDKIQLGQFVHVA L+ GS            KRR
Sbjct: 52   GFFLKLSDSLHSAYVSVSDEDVDLISTDKIQLGQFVHVAGLDAGSPVPVLRGIKPIPKRR 111

Query: 1875 ECVGTPVDLISSDVL----WXXXXXXXXXXXXXXXXLRRVSLGNVKNXXXXXXXXXXXXX 1708
             CVG P DL+SSD+L                      +R+SL + +              
Sbjct: 112  PCVGNPKDLVSSDLLPIRSSAVHFSRTTKKDKLKTKAKRLSLDSAR-------------- 157

Query: 1707 RKGWDRS-----GCPPGSSH--SASVSATKNASSEKNLTLKHQIFSPLKQV----VPHRR 1561
             + WD+S       PP SS   +  +S + N SS+K  + K    S    V    +  + 
Sbjct: 158  -RAWDQSPTPKKTTPPKSSRFKTKQISTSSNVSSDKKASFKIDSSSKRSSVSISPLKSKN 216

Query: 1560 SFSEKDIAVKP 1528
              S   +A KP
Sbjct: 217  EISSPKLACKP 227


>emb|CBI18661.3| unnamed protein product [Vitis vinifera]
          Length = 566

 Score =  345 bits (885), Expect = 3e-92
 Identities = 189/361 (52%), Positives = 239/361 (66%), Gaps = 1/361 (0%)
 Frame = -1

Query: 1215 STLKPLNVGTSPLQNKNVIDSSSPASKPVRKETKQSAECTSHLSKVPLSFKTWSDAKISW 1036
            S+ K  +V  SPL++KN I S   A KP++K++K               FKTWSD  ISW
Sbjct: 220  SSSKRSSVSISPLKSKNEISSPKLACKPLQKDSK---------------FKTWSDQNISW 264

Query: 1035 GSLPSRVHDLGKEVTFQRNARFLDALYALQETSAMENILQCMRMFAELCESSDKESSLPL 856
             SLP R+HDLGK+    RN  FL A+ AL+E SA E ++QCM MFAELCESS K+S+ PL
Sbjct: 265  DSLPPRIHDLGKDAASYRNVAFLAAVNALEEASAAEGVIQCMSMFAELCESSQKDSAGPL 324

Query: 855  VEEYLNFHQTMQRVATAVNSLLSVRFNE-EDGTCYSSLCSLSETCKEITDKNALSWVQAA 679
            VE++LN HQ MQ+ A ++N+LLS R  E E  +C SS   L+E CK   +KNA+SWVQAA
Sbjct: 325  VEQFLNLHQDMQKAAMSINALLSTRLAEVESSSCCSSQPPLAEACKNFNNKNAISWVQAA 384

Query: 678  VVTNLSKFNLFTKEDKRGDFNSEKCHYVILENTPIKIDVENHSPDKKRNSKKQGAVMSGS 499
            + T+LSKF LF K+D R   + EKCHY++LEN+P ++  ENHSP  K + +  G +   S
Sbjct: 385  LETDLSKFCLFRKQDNREIRSGEKCHYIVLENSP-EMKSENHSPQNKPSPRNNGGLSDSS 443

Query: 498  SLKTSSPYHSKQRLPXXXXXXXXXXXXXKGNGLTVAANLAQKLLSTSQAWFLDYLEASLE 319
            +    SP  S +R               KG+GL   ANLA+KLLS S+ WFL YLE SL 
Sbjct: 444  AKGLPSP--SWRRFSASKRMSAKREEWSKGSGLRETANLAKKLLSVSRGWFLKYLEDSLN 501

Query: 318  KGFGLQKGEKDSEIACLLGQLKRVNQWLDDSVTDQNGTNERIEGLKKKLYGFLLDNVDSA 139
            KGFGL++ E  +EIACLLGQLKRVNQWLD SV D    +ERIEGL+KKLYGFLL++VDSA
Sbjct: 502  KGFGLKREEGGAEIACLLGQLKRVNQWLDGSVEDGMEVDERIEGLRKKLYGFLLEHVDSA 561

Query: 138  V 136
            V
Sbjct: 562  V 562



 Score =  101 bits (252), Expect = 7e-19
 Identities = 79/205 (38%), Positives = 101/205 (49%), Gaps = 2/205 (0%)
 Frame = -1

Query: 2055 GFYLRLSDSLHSAYVSVSDDDVELILTDKIQLGQFVHVARLEDGSXXXXXXXXXXXXKRR 1876
            GF+L+LSDSLHSAYVSVSD+DV+LI TDKIQLGQFVHVA L+ GS            KRR
Sbjct: 52   GFFLKLSDSLHSAYVSVSDEDVDLISTDKIQLGQFVHVAGLDAGSPVPVLRGIKPIPKRR 111

Query: 1875 ECVGTPVDLISSDVLWXXXXXXXXXXXXXXXXLRRVSLGNVKN-XXXXXXXXXXXXXRKG 1699
             CVG P DL+SSD+L                  RR S+GN                 R+ 
Sbjct: 112  PCVGNPKDLVSSDLL-PIRSSAAKRSVLQDTKSRRASIGNGPTLECLELRRLSLDSARRA 170

Query: 1698 WDRSGCPPGSS-HSASVSATKNASSEKNLTLKHQIFSPLKQVVPHRRSFSEKDIAVKPPS 1522
            WD+S  P  ++   +S   TK  S+  N+     + SP       + SF + D + K  S
Sbjct: 171  WDQSPTPKKTTPPKSSRFKTKQISTSSNVCF---LVSPFSVSSDKKASF-KIDSSSKRSS 226

Query: 1521 PRISPHKQVLPHRKSLSERDITVKP 1447
              ISP    L  +  +S   +  KP
Sbjct: 227  VSISP----LKSKNEISSPKLACKP 247


>ref|XP_003532890.1| PREDICTED: uncharacterized protein LOC100782697 [Glycine max]
          Length = 569

 Score =  244 bits (624), Expect = 5e-62
 Identities = 161/386 (41%), Positives = 216/386 (55%), Gaps = 15/386 (3%)
 Frame = -1

Query: 1248 PPSINQLSEKKSTLKPLNV------GTSPLQNKNV-IDSSSPASKPVRKETKQSAECT-- 1096
            P S+N + +K  ++KP +         SPL+NKN  +   S    P RK T  S      
Sbjct: 186  PTSLNVIDKKVVSVKPDSPLKSPTSSVSPLKNKNENLCPKSLTITPPRKSTSTSTRSPCG 245

Query: 1095 ----SHLSKVPLSFKTWS-DAKISWGSLPSRVHDLGKEVTFQRNARFLDALYALQETSAM 931
                S L KVPL+FKTW  D    W  +   + DLGK+V   R   FL A+ +L+E SA 
Sbjct: 246  GTVHSPLVKVPLNFKTWCYDTPACWEDVSLPMCDLGKQVVTHRTVAFLAAVRSLEEASAT 305

Query: 930  ENILQCMRMFAELCESSDKESSLPLVEEYLNFHQTMQRVATAVNSLLSVRFNEEDGTCYS 751
            ++++QCM M+AELC+S    S   LV+++L  H ++QRV    +SLL+    E   + +S
Sbjct: 306  DSVIQCMCMYAELCQSGQTHSPGSLVKQFLELHLSLQRVTVLFDSLLNP-LPETKSSSHS 364

Query: 750  SL-CSLSETCKEITDKNALSWVQAAVVTNLSKFNLFTKEDKRGDFNSEKCHYVILENTPI 574
            +L C   + CK  T KNA+SWVQAAV TNLSKFNLF  + K    N EKCHYV++ENTP 
Sbjct: 365  TLQCLTEDACKVPTRKNAISWVQAAVGTNLSKFNLFETQAKSEVLNGEKCHYVVIENTPE 424

Query: 573  KIDVENHSPDKKRNSKKQGAVMSGSSLKTSSPYHSKQRLPXXXXXXXXXXXXXKGNGLTV 394
             I+ E      K+N   Q   +  S+ K      SK+ L              K   L  
Sbjct: 425  DINTETSPAQNKQNRVTQANHLPNSTAKRLP--SSKRNLLAAKNKDADKHDRSKEIKLKE 482

Query: 393  AANLAQKLLSTSQAWFLDYLEASLEKGFGLQKGEKDSEIACLLGQLKRVNQWLDDSVTDQ 214
            AA+LA+KLL  S  WFL YLE SL   FGL K E ++EIA LLGQLK+VN WL D++   
Sbjct: 483  AASLAEKLLEASSEWFLKYLEESLSNEFGL-KNEGNTEIASLLGQLKQVNHWL-DNLAGG 540

Query: 213  NGTNERIEGLKKKLYGFLLDNVDSAV 136
            +  + R+E L+K LY FLL++V+SAV
Sbjct: 541  DKVDHRVEKLRKNLYRFLLEHVNSAV 566



 Score = 85.5 bits (210), Expect = 5e-14
 Identities = 66/207 (31%), Positives = 93/207 (44%), Gaps = 3/207 (1%)
 Frame = -1

Query: 2055 GFYLRLSDSLHSAYVSVSDDDVELILTDKIQLGQFVHVARLEDGSXXXXXXXXXXXXKRR 1876
            G++L+LSDSLHSAYVSV D D +LI   K+ LGQFV+V RL+  S            +RR
Sbjct: 48   GYFLKLSDSLHSAYVSVPDADADLICAGKLHLGQFVYVTRLDAASPVPLVRGLNVLPRRR 107

Query: 1875 ECVGTPVDLISSDVLWXXXXXXXXXXXXXXXXLRRVSLGNVKNXXXXXXXXXXXXXRKGW 1696
             CVG P +L+ S+ L                  +R  +   +              R+ W
Sbjct: 108  PCVGNPTELVPSERL----------CVATPKKKKREEVVKKEKGLEMMRRLSLDSSRRVW 157

Query: 1695 DRSGCPPGSSHSASVSATKNASSEKNLTLKHQIFSPLKQVVPHRRSFSEKDIAVKPPSPR 1516
            D+S  P     +AS S+  N     NL  K    +P    V       +K ++VKP SP 
Sbjct: 158  DQS--PVAKRKTASASSLSN-----NLKAKKSNSNPTSLNV-----IDKKVVSVKPDSPL 205

Query: 1515 ISPHKQVLP---HRKSLSERDITVKPP 1444
             SP   V P     ++L  + +T+ PP
Sbjct: 206  KSPTSSVSPLKNKNENLCPKSLTITPP 232


>ref|XP_003595829.1| hypothetical protein MTR_2g062300 [Medicago truncatula]
            gi|124360104|gb|ABN08120.1| Legume lectin, beta domain
            [Medicago truncatula] gi|355484877|gb|AES66080.1|
            hypothetical protein MTR_2g062300 [Medicago truncatula]
          Length = 585

 Score =  234 bits (598), Expect = 5e-59
 Identities = 153/376 (40%), Positives = 209/376 (55%), Gaps = 5/376 (1%)
 Frame = -1

Query: 1239 INQLSEKKSTLKPLNVGTSPLQNKNVIDSSSPASKPVRKET-KQSAECTSHLSKVPLSFK 1063
            ++ L  K   L P   GT P ++   I        P+ K     +    S L KVPL FK
Sbjct: 224  VSPLKNKNENLCPKTTGTPPRKSATTI--------PIPKSPCAGNGSVPSKLVKVPLKFK 275

Query: 1062 TWSDAKISWGSLPSRVHDLGKEVTFQRNARFLDALYALQETSAMENILQCMRMFAELCES 883
            TW D   SW +LP  + +LGK+V   RN  FL A+ +L+E SA + +LQCM MFAELCES
Sbjct: 276  TWYDKSGSWDNLPPPICNLGKQVVTHRNVAFLAAVRSLEEASAADTVLQCMCMFAELCES 335

Query: 882  SDKESSLPLVEEYLNFHQTMQRVATAVNSLLSVRFNEEDGTCYSSLCSLSE-TCKEITDK 706
                S+  LV+++L  H ++QRV    +SLLS    E   T +++L SL E  C   T K
Sbjct: 336  RQTLSAGLLVKQFLELHLSLQRVTVVFDSLLSTP-PETKPTGHTTLQSLVEDACNVPTRK 394

Query: 705  NALSWVQAAVVTNLSKFNLFTKEDKRGDFNSEKCHYVILENTPIKIDVENHSPDKKRNSK 526
            NA  WVQAA+ TNLSK NL+  ++K    N EK H+V++EN       E  + ++     
Sbjct: 395  NATFWVQAALYTNLSKLNLYKTQEKSKVLNGEKSHFVVIENGN---SHEERNAEESTLQN 451

Query: 525  KQGAVMSGSSLKTSSPYH---SKQRLPXXXXXXXXXXXXXKGNGLTVAANLAQKLLSTSQ 355
            KQ  V   + L+ S+      SK+ L              K + L VA +L++KLL+ S+
Sbjct: 452  KQSRVAQANPLQNSTGKRISSSKRNLLVAKNKDTEKRDQSKESELKVAVSLSEKLLAASR 511

Query: 354  AWFLDYLEASLEKGFGLQKGEKDSEIACLLGQLKRVNQWLDDSVTDQNGTNERIEGLKKK 175
             WFL YLE S+   FGL K E  SE ACLLGQL+ VN WLD+ V D    ++R+E L+K 
Sbjct: 512  EWFLKYLEESIGNEFGL-KNEGSSEAACLLGQLREVNHWLDNLV-DGGKVDDRVEKLRKN 569

Query: 174  LYGFLLDNVDSAVAQT 127
            LY FLL++V+SAVA +
Sbjct: 570  LYRFLLEHVNSAVASS 585



 Score = 83.6 bits (205), Expect = 2e-13
 Identities = 72/219 (32%), Positives = 99/219 (45%), Gaps = 15/219 (6%)
 Frame = -1

Query: 2058 SGFYLRLSDSLHSAYVSVSDDDVELILTDKIQLGQFVHVARLED--GSXXXXXXXXXXXX 1885
            +G++L+LSDSLHSAYVSVSD D ELI +DK+QLGQFV+V R ++   S            
Sbjct: 45   TGYFLKLSDSLHSAYVSVSDSDAELIRSDKLQLGQFVYVTRFDNDASSSVPRVSGLKTIS 104

Query: 1884 KRRECVGTPVDLISSDVLWXXXXXXXXXXXXXXXXLRRVSLGNVKNXXXXXXXXXXXXXR 1705
            KRR CVG PVDL+S+D L                        NV               R
Sbjct: 105  KRRACVGNPVDLVSTDSLRLATAKLNGNVKKKKKINGNKD-NNVVVNVEMRRLSLDSSTR 163

Query: 1704 KGWD--------RSGCPPGSSHSASVSATKNASS-----EKNLTLKHQIFSPLKQVVPHR 1564
            + WD        +S   P S+ S+  + +K+AS+     +K +++K    SPLK   P  
Sbjct: 164  RVWDHASLVSKTKSSSSPSSNSSSRFTFSKSASTSPNVIDKKVSMKIDSHSPLKS--PST 221

Query: 1563 RSFSEKDIAVKPPSPRISPHKQVLPHRKSLSERDITVKP 1447
               S     +K  +  + P     P RKS +   I   P
Sbjct: 222  SIVS----PLKNKNENLCPKTTGTPPRKSATTIPIPKSP 256


>ref|NP_180751.1| uncharacterized protein [Arabidopsis thaliana]
            gi|4887744|gb|AAD32280.1| hypothetical protein
            [Arabidopsis thaliana] gi|330253509|gb|AEC08603.1|
            uncharacterized protein [Arabidopsis thaliana]
          Length = 585

 Score =  192 bits (487), Expect = 4e-46
 Identities = 129/379 (34%), Positives = 194/379 (51%), Gaps = 14/379 (3%)
 Frame = -1

Query: 1221 KKSTLKPLNVGTSPLQNKNVIDSSSPASKPVRKETKQSAECTSH-------LSKVPLSFK 1063
            K    K LN  T  L+++ V+  +SP S  V +  K   +  S        L KVP S  
Sbjct: 238  KSPISKHLNCETPALRSRYVVKPASPIS--VTRSPKDGIKSLSKAVTPPVALFKVPSSHM 295

Query: 1062 TWSDAKISWGSLPSRVHDLGKEVTFQRNARFLDALYALQETSAMENILQCMRMFAELCES 883
            TWSD ++SW  LP  +  LGKEV+  R      A+ AL+E SAME++L  ++ FAELC+S
Sbjct: 296  TWSDQRMSWSGLPKTIQLLGKEVSSHRQVAVSAAVSALEEASAMESVLFSLQAFAELCDS 355

Query: 882  SDKESSLPLVEEYLNFHQTMQRVATAVNSLLSVRFNEEDGTCYSSLCSLSETCKEITDKN 703
            + K S+  +V  +L+ +   Q    AV+ LL+   N             + +C+ + +KN
Sbjct: 356  TKKLSTGQVVARFLDIYHNTQNTCKAVHRLLTQNRN-------------NGSCRLVVNKN 402

Query: 702  ALSWVQAAVVTNLSKFNLFTKEDKRGDFNSEKCHYVILENTPIKIDVENHSPDK------ 541
            A SWVQAAVVT  S+FNLF +  K+GD  +   HY++++N+  K++ +  +  +      
Sbjct: 403  ATSWVQAAVVTGFSQFNLFKEPGKKGD--AADHHYIVMQNSSEKLNPKETTSSRTPSYKG 460

Query: 540  -KRNSKKQGAVMSGSSLKTSSPYHSKQRLPXXXXXXXXXXXXXKGNGLTVAANLAQKLLS 364
             K  + K  +V   SSL+  S                          L  + +LA +L+ 
Sbjct: 461  AKPPATKHCSVSDRSSLEGKS-------------------------SLKDSTSLADELVQ 495

Query: 363  TSQAWFLDYLEASLEKGFGLQKGEKDSEIACLLGQLKRVNQWLDDSVTDQNGTNERIEGL 184
             S  WFL YLE SL KG  L K E+ +    LL  LK +N WLDD   ++  TNE++E L
Sbjct: 496  VSSQWFLKYLENSLNKGSFLVKTEETNGKESLLVHLKALNHWLDDLNLNRTETNEKVEDL 555

Query: 183  KKKLYGFLLDNVDSAVAQT 127
            +KKL  FLL++++S +  T
Sbjct: 556  RKKLQRFLLEHIESVIGDT 574



 Score = 66.2 bits (160), Expect = 3e-08
 Identities = 37/78 (47%), Positives = 51/78 (65%), Gaps = 4/78 (5%)
 Frame = -1

Query: 2052 FYLRLSDSLHSAYVSVS-DDDVELILTDKIQLGQFVHVA---RLEDGSXXXXXXXXXXXX 1885
            F++++SDSLH+AYV+VS  DD +LI +D+IQLGQF+++     +E G             
Sbjct: 54   FFVKVSDSLHAAYVAVSTGDDADLIRSDEIQLGQFIYICGGLHVEKGCPVPVIRGLKPVP 113

Query: 1884 KRRECVGTPVDLISSDVL 1831
            KRR CVG P DL SSD+L
Sbjct: 114  KRRMCVGNPSDLYSSDLL 131