BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00019882
         (1383 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAN78085.1| hypothetical protein VITISV_034041 [Vitis vinifera]   389   e-105
ref|XP_002277934.1| PREDICTED: GDSL esterase/lipase At5g55050 [V...   386   e-105
gb|AFK33479.1| unknown [Lotus japonicus]                              383   e-104
ref|XP_002317343.1| predicted protein [Populus trichocarpa] gi|2...   376   e-102
ref|XP_003617573.1| GDSL esterase/lipase [Medicago truncatula] g...   375   e-101

>emb|CAN78085.1| hypothetical protein VITISV_034041 [Vitis vinifera]
          Length = 364

 Score =  389 bits (998), Expect = e-105
 Identities = 188/356 (52%), Positives = 247/356 (69%), Gaps = 1/356 (0%)
 Frame = +2

Query: 272  ISFLSITCLSKAQDSSSRKVPAMYVFGDSLLDVGNNNYLAVSLAKANFPHNGVDFPTGKP 451
            I F S+   S+AQ       PA+++FGDSL+DVGNNN+L +SLAKA+FPHNGVDFP  KP
Sbjct: 14   IVFSSLFIFSEAQ-----LAPALFMFGDSLVDVGNNNHLKLSLAKADFPHNGVDFPGKKP 68

Query: 452  TGRFSNGKNAADFIAEKLGLITASPYLSIKNSKSDKTNAMIXXXXXXXXXXXXXNGTDEL 631
            TGRF NGKNAADF+AEKLGL +A PYLS+ +       + +             +GTD L
Sbjct: 69   TGRFCNGKNAADFLAEKLGLPSAPPYLSLISKSKSSNESFVAGVSFASGGAGIFDGTDAL 128

Query: 632  FKQSIPLTKXXXXXXXXXXXXXXXMGSAAAQKHLSNSIFPIVIGSNDLFGYFKSDSDVSK 811
            +KQS+PL K               +GSA AQ+HLS S+F +VIGSND+ GY+ SDS    
Sbjct: 129  YKQSLPLKKQVDYYATVYERLVQQLGSAGAQEHLSKSVFAVVIGSNDILGYYGSDSSTRN 188

Query: 812  KSTPQQYIDSMLSAVSGVLKRLRDLGARKFVIVGIGAIGCTPSQRIQNKTQVCKEEVNFW 991
            K+TPQQ++DSM + +   LK + +LGARKF +VG+GA+GC PSQR +  T+ C EE N+W
Sbjct: 189  KTTPQQFVDSMAATLKEQLKXMYNLGARKFAMVGVGAVGCCPSQRNKKSTEECSEEANYW 248

Query: 992  ARKYNDGLTSVLKAFKSEFKDINYSYFDAYSVFVDFIQNPSTYGFTEVRSACCGLGDLKA 1171
            + KYN+ L S+L+   SE K ++YSYFD YSV ++ IQ P+ YGF EV++ACCGLG+L A
Sbjct: 249  SVKYNERLKSLLQELISELKGMSYSYFDTYSVMLNLIQKPAAYGFKEVKAACCGLGNLNA 308

Query: 1172 KVPCIPISTYCSNRNDHLFWDLYHPTQKAAGLLVDIIFDGS-KYVTPITINQLIAA 1336
              PC+PISTYCSNR DH+FWDLYHPT+ AA ++V  IF+G+ +Y  P  + QL+AA
Sbjct: 309  DFPCLPISTYCSNRKDHVFWDLYHPTEAAASIVVQNIFNGTQEYTFPXNLRQLVAA 364


>ref|XP_002277934.1| PREDICTED: GDSL esterase/lipase At5g55050 [Vitis vinifera]
            gi|297737455|emb|CBI26656.3| unnamed protein product
            [Vitis vinifera]
          Length = 364

 Score =  386 bits (991), Expect = e-105
 Identities = 192/357 (53%), Positives = 252/357 (70%), Gaps = 2/357 (0%)
 Frame = +2

Query: 272  ISFLSITCLSKAQDSSSRKVPAMYVFGDSLLDVGNNNYLAVSLAKANFPHNGVDFPTGKP 451
            I F S+   S+AQ       PA+++FGDSL+DVGNNN+L +SLAKA+FPHNGVDFP  KP
Sbjct: 14   IVFSSLFIFSEAQ-----LAPALFMFGDSLVDVGNNNHLKLSLAKADFPHNGVDFPGKKP 68

Query: 452  TGRFSNGKNAADFIAEKLGLITASPYLSIKNSKSDKTNA-MIXXXXXXXXXXXXXNGTDE 628
            TGRF NGKNAADF+AEKLGL +A PYLS+  SKS+ +NA  +             +GTD 
Sbjct: 69   TGRFCNGKNAADFLAEKLGLPSAPPYLSLI-SKSNLSNASFVAGVSFASGGAGIFDGTDA 127

Query: 629  LFKQSIPLTKXXXXXXXXXXXXXXXMGSAAAQKHLSNSIFPIVIGSNDLFGYFKSDSDVS 808
            L+KQS+PL K               +GSA AQ+HLS S+F +VIGSND+ GY+ SDS   
Sbjct: 128  LYKQSLPLKKQVAYYATVYERLVQQLGSAGAQEHLSKSVFAVVIGSNDILGYYGSDSSTR 187

Query: 809  KKSTPQQYIDSMLSAVSGVLKRLRDLGARKFVIVGIGAIGCTPSQRIQNKTQVCKEEVNF 988
             K+ PQQ++DSM + +   LK + +LGARKF +VG+GA+GC PSQR +  T+ C EE N+
Sbjct: 188  NKTAPQQFVDSMAATLKEQLKGMYNLGARKFAMVGVGAVGCCPSQRNKKSTEECSEEANY 247

Query: 989  WARKYNDGLTSVLKAFKSEFKDINYSYFDAYSVFVDFIQNPSTYGFTEVRSACCGLGDLK 1168
            W+ KYN+ L S+L+   SE K ++YSYFD YSV ++ IQ P+ YGF EV++ACCGLG+L 
Sbjct: 248  WSVKYNERLKSLLQELISELKGMSYSYFDTYSVMLNLIQKPAAYGFKEVKAACCGLGNLN 307

Query: 1169 AKVPCIPISTYCSNRNDHLFWDLYHPTQKAAGLLVDIIFDGS-KYVTPITINQLIAA 1336
            A  PC+PISTYCSNR DH+FWDLYHPT+ AA ++V  IF+G+ +Y  P+ + QL+AA
Sbjct: 308  ADFPCLPISTYCSNRKDHVFWDLYHPTEAAASIVVQNIFNGTQEYTFPMNLRQLVAA 364


>gb|AFK33479.1| unknown [Lotus japonicus]
          Length = 361

 Score =  383 bits (984), Expect = e-104
 Identities = 196/363 (53%), Positives = 254/363 (69%), Gaps = 3/363 (0%)
 Frame = +2

Query: 257  MLIIVISFLSITCLSKAQDSSSRKVPAMYVFGDSLLDVGNNNYLAVSLAKANFPHNGVDF 436
            + + VIS +  + +S    S ++ VPA+YVFGDSL+DVGNNNYL +S+AKAN  H GVDF
Sbjct: 5    LYVFVISVVIFSSVS----SEAQMVPAVYVFGDSLVDVGNNNYLTLSIAKANHRHYGVDF 60

Query: 437  PTGKPTGRFSNGKNAADFIAEKLGLITASPYLSI--KNSKSDKTNAMIXXXXXXXXXXXX 610
            P  KPTGRFSNGKNAADFIAEKLGL T+ PYLS+  K +K+  T++ +            
Sbjct: 61   PNQKPTGRFSNGKNAADFIAEKLGLPTSPPYLSLVSKANKNSNTSSFMDGVSFASAGAEI 120

Query: 611  XNGTDELFKQSIPLTKXXXXXXXXXXXXXXXMGSAAAQKHLSNSIFPIVIGSNDLFGYFK 790
             +GTDE ++QSIPLTK               +G+ A QKHLS SIF +VIGSND+FGY  
Sbjct: 121  FDGTDEHYRQSIPLTKQVDYYTLVHEQMTREVGTPALQKHLSRSIFAVVIGSNDIFGYSG 180

Query: 791  SDSDVSKKSTPQQYIDSMLSAVSGVLKRLRDLGARKFVIVGIGAIGCTPSQRIQNKTQVC 970
            S SD+ KK+TPQQY+DSM  ++   L+RL D GARKF I G+GA+GC P+ R++N T+ C
Sbjct: 181  S-SDLRKKNTPQQYVDSMAFSLKVQLQRLYDYGARKFEITGVGALGCCPTFRVKNNTE-C 238

Query: 971  KEEVNFWARKYNDGLTSVLKAFKSEFKDINYSYFDAYSVFVDFIQNPSTYGFTEVRSACC 1150
              EVN+W+ KYN GL S+LK ++SE   I YSYFD Y+V  D IQNP++YGF +V+ ACC
Sbjct: 239  VTEVNYWSVKYNQGLQSMLKEWQSENGGIIYSYFDTYTVINDLIQNPASYGFADVKEACC 298

Query: 1151 GLGDLKAKVPCIPISTYCSNRNDHLFWDLYHPTQKAAGLLVDIIFDG-SKYVTPITINQL 1327
            GLG+L AK PC+P+S  C NR DH+FWD +HPT+ A+   V+ IFDG S Y +PI + QL
Sbjct: 299  GLGELNAKAPCVPVSKLCPNRQDHIFWDQFHPTEAASRSFVERIFDGSSSYTSPINMRQL 358

Query: 1328 IAA 1336
            +AA
Sbjct: 359  VAA 361


>ref|XP_002317343.1| predicted protein [Populus trichocarpa] gi|222860408|gb|EEE97955.1|
            predicted protein [Populus trichocarpa]
          Length = 361

 Score =  376 bits (966), Expect = e-102
 Identities = 194/370 (52%), Positives = 248/370 (67%), Gaps = 2/370 (0%)
 Frame = +2

Query: 227  MGRKIIIDSYMLIIVISFLSITCLSKAQDSSSRKVPAMYVFGDSLLDVGNNNYLAVSLAK 406
            MG K  + S++    + F  +  L  A   +++ VPA++VFGDSL+DVGNNNYL VS+AK
Sbjct: 1    MGNKSFLPSFL----VGFALVLSLKFA---NAQMVPAIFVFGDSLVDVGNNNYLPVSVAK 53

Query: 407  ANFPHNGVDFPTGKPTGRFSNGKNAADFIAEKLGLITASPYLSIKNSKSDKTNAMIXXXX 586
            A+FPHNG+DFPT K TGRFSNGKNAADF+A+K+GL T+ PYLS+       T++ +    
Sbjct: 54   ADFPHNGIDFPTKKATGRFSNGKNAADFLAQKVGLPTSPPYLSVS---PQNTSSFMTGVS 110

Query: 587  XXXXXXXXXNGTDELFKQSIPLTKXXXXXXXXXXXXXXXMGSAAAQKHLSNSIFPIVIGS 766
                     NGTD    Q+IPLTK               +G + AQK LS S+F IVIGS
Sbjct: 111  FASGGAGIFNGTDRTLGQAIPLTKQVGNYESVYGKLIQRLGLSGAQKRLSKSLFVIVIGS 170

Query: 767  NDLFGYFKSDSDVSKKSTPQQYIDSMLSAVSGVLKRLRDLGARKFVIVGIGAIGCTPSQR 946
            ND+F Y  S SD+ KKSTPQQY+DSM+  + G+LKRL   GARKFV  GIG +GC PSQR
Sbjct: 171  NDIFDYSGS-SDLQKKSTPQQYVDSMVLTIKGLLKRLHTSGARKFVFAGIGPLGCIPSQR 229

Query: 947  IQNKTQV-CKEEVNFWARKYNDGLTSVLKAFKSEFKDINYSYFDAYSVFVDFIQNPSTYG 1123
            I+N+T   C E  N  A  YN GL S+L+  KS    I+YSYFD Y++  + IQNP+TYG
Sbjct: 230  IKNQTDHGCNEGSNLMAVAYNKGLNSILQELKSNLNAISYSYFDTYALMHNIIQNPATYG 289

Query: 1124 FTEVRSACCGLGDLKAKVPCIPISTYCSNRNDHLFWDLYHPTQKAAGLLVDIIFDGS-KY 1300
            FTEV +ACCG G L A++PC+PIS YCSNR DH+FWDLYHPT+  A +LVD IF+G  +Y
Sbjct: 290  FTEVEAACCGRGKLNAQIPCLPISKYCSNRRDHVFWDLYHPTETTASILVDAIFNGPLQY 349

Query: 1301 VTPITINQLI 1330
              P+ + QL+
Sbjct: 350  TFPMNVRQLV 359


>ref|XP_003617573.1| GDSL esterase/lipase [Medicago truncatula]
            gi|355518908|gb|AET00532.1| GDSL esterase/lipase
            [Medicago truncatula]
          Length = 362

 Score =  375 bits (963), Expect = e-101
 Identities = 186/344 (54%), Positives = 246/344 (71%), Gaps = 3/344 (0%)
 Frame = +2

Query: 314  SSSRKVPAMYVFGDSLLDVGNNNYLAVSLAKANFPHNGVDFPTGKPTGRFSNGKNAADFI 493
            S ++ VPA+YVFGDSL+DVGNNNYL +S+AKAN  H G+DF   KPTGRFSNGKNAADFI
Sbjct: 21   SMAQMVPAVYVFGDSLVDVGNNNYLTLSIAKANHRHYGIDFLNHKPTGRFSNGKNAADFI 80

Query: 494  AEKLGLITASPYLSI--KNSKSDKTNAMIXXXXXXXXXXXXXNGTDELFKQSIPLTKXXX 667
             EKLGL T+ PYLS+  K +K++   + I             +GTDE ++QS+PLTK   
Sbjct: 81   GEKLGLATSPPYLSLISKGNKNENNASFINGVSFASAGAGIFDGTDERYRQSLPLTKQVN 140

Query: 668  XXXXXXXXXXXXMGSAAAQKHLSNSIFPIVIGSNDLFGYFKSDSDVSKKSTPQQYIDSML 847
                        +G++A QKHLS SIF +VIG+NDLFGYF+S S++ KK+TPQQY+DSML
Sbjct: 141  YYTNVYEELIREVGASALQKHLSKSIFAVVIGNNDLFGYFES-SELRKKNTPQQYVDSML 199

Query: 848  SAVSGVLKRLRDLGARKFVIVGIGAIGCTPSQRIQNKTQVCKEEVNFWARKYNDGLTSVL 1027
             ++   L+RL D G RKF I G+GA+GC P  R++N+T+ C  E N+W+ +YN GL S+L
Sbjct: 200  FSLKLQLQRLYDNGGRKFEIAGVGALGCCPMFRLKNQTE-CVVETNYWSVQYNKGLQSML 258

Query: 1028 KAFKSEFKDINYSYFDAYSVFVDFIQNPSTYGFTEVRSACCGLGDLKAKVPCIPISTYCS 1207
            K ++SE + I YSYFD Y    D IQNP++YGFT+V++ACCGLG+L A+ PC+P+S  C 
Sbjct: 259  KEWQSENQGIIYSYFDTYVAMNDLIQNPASYGFTDVKAACCGLGELNARAPCLPVSHLCP 318

Query: 1208 NRNDHLFWDLYHPTQKAAGLLVDIIFDGSK-YVTPITINQLIAA 1336
            NR DH+FWD +HPT+ A+ + VD IFDGS  Y +PI + QL+AA
Sbjct: 319  NRQDHIFWDQFHPTEAASRIFVDKIFDGSSTYTSPINMRQLVAA 362