BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00018735
         (1314 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAN70740.1| hypothetical protein VITISV_013920 [Vitis vinifera]   459   e-127
emb|CBI28884.3| unnamed protein product [Vitis vinifera]              454   e-125
ref|XP_002276681.1| PREDICTED: GDSL esterase/lipase At2g04570 [V...   454   e-125
ref|XP_002271851.1| PREDICTED: GDSL esterase/lipase At2g04570-li...   439   e-121
ref|XP_004140128.1| PREDICTED: GDSL esterase/lipase At2g04570-li...   436   e-120

>emb|CAN70740.1| hypothetical protein VITISV_013920 [Vitis vinifera]
          Length = 349

 Score =  459 bits (1182), Expect = e-127
 Identities = 217/313 (69%), Positives = 255/313 (81%)
 Frame = +1

Query: 331  GNNNQISTVLKSNFEPYGRDFYGGKPTGRFSNGKIPPDFISEAFGLKPTVPAYLDPAYNI 510
            GNNN+ISTVLKSNFEPYGRDF GG+PTGRFSNG+IPPDFISEAFGLKPTVPAYLDP YNI
Sbjct: 39   GNNNRISTVLKSNFEPYGRDFTGGRPTGRFSNGRIPPDFISEAFGLKPTVPAYLDPNYNI 98

Query: 511  SDFVDGVNFASAGTGYDTATSDVLSVIPFWKELDYYKEYQSKLKAYVGDAKANEITSEAL 690
            SDF  GV FASAGTGYD  TSDVLSVIP WKEL+YYKEYQ KL+AY+G  KANEI SE+L
Sbjct: 99   SDFATGVCFASAGTGYDNQTSDVLSVIPLWKELEYYKEYQXKLRAYLGQEKANEILSESL 158

Query: 691  YLLSLGTNDFLENYYTVPTRRDQFTVDKYQDYLCGIADKFIRELYALGAKKISFGGLPPM 870
            YL+SLGTNDFLENYY    R  Q+TV +Y+D+L GIA  FI+E+Y+LGA+K+S GGLPPM
Sbjct: 159  YLMSLGTNDFLENYYIFSGRSSQYTVPQYEDFLVGIAGNFIKEIYSLGARKVSLGGLPPM 218

Query: 871  GCLPLERTADFFRGNGDVCNEDYNKVALDFXXXXXXXXXXXXQELSGLRLVLSNPYFILQ 1050
            GCLPLERT +FF   G  C E YN VA++F            + L G+++VLSNPYFILQ
Sbjct: 219  GCLPLERTTNFF--GGSECIERYNNVAMEFNGKLNTLVGKLNKXLPGIKVVLSNPYFILQ 276

Query: 1051 HIVKKPSLFGFDVAAVACCSSGLFEMSYLCDRMNPFTCTDADKYVFWDSFHPTQKTNQIV 1230
             I++KPS +G++ AAVACC++G+FEM YLC+R N  TC DA KYVFWDSFHPT+KTN I+
Sbjct: 277  XIIRKPSSYGYENAAVACCATGMFEMGYLCNRYNMLTCPDASKYVFWDSFHPTEKTNGII 336

Query: 1231 SEHLIKNVLHVFL 1269
            S+H++K VL VFL
Sbjct: 337  SDHVVKTVLKVFL 349


>emb|CBI28884.3| unnamed protein product [Vitis vinifera]
          Length = 658

 Score =  454 bits (1168), Expect = e-125
 Identities = 211/313 (67%), Positives = 255/313 (81%)
 Frame = +1

Query: 331  GNNNQISTVLKSNFEPYGRDFYGGKPTGRFSNGKIPPDFISEAFGLKPTVPAYLDPAYNI 510
            GNNNQIST+ KSNFEPYGR+F GG+PTGRFSNG+I  DFISEAFGLKPTVPAYLDP Y+I
Sbjct: 348  GNNNQISTIAKSNFEPYGRNFPGGRPTGRFSNGRISTDFISEAFGLKPTVPAYLDPTYSI 407

Query: 511  SDFVDGVNFASAGTGYDTATSDVLSVIPFWKELDYYKEYQSKLKAYVGDAKANEITSEAL 690
             DF  GV+FASAG+GYD ATSDVLSVIP WKEL+YYK+YQ++L+AY+G  KANE+ SEAL
Sbjct: 408  KDFATGVSFASAGSGYDNATSDVLSVIPLWKELEYYKDYQTELRAYLGVKKANEVLSEAL 467

Query: 691  YLLSLGTNDFLENYYTVPTRRDQFTVDKYQDYLCGIADKFIRELYALGAKKISFGGLPPM 870
            Y++SLGTNDFLENYY  P R  QFT+ +Y+D+L GIA  F+ +LY LGA+KIS GGLPPM
Sbjct: 468  YVMSLGTNDFLENYYAFPNRSSQFTIKQYEDFLIGIAGHFVHQLYGLGARKISVGGLPPM 527

Query: 871  GCLPLERTADFFRGNGDVCNEDYNKVALDFXXXXXXXXXXXXQELSGLRLVLSNPYFILQ 1050
            GC+PLERT +F   NG  C E+YN VALDF            +EL G ++VLSNPY+IL 
Sbjct: 528  GCMPLERTTNFM--NGAECVEEYNNVALDFNWKLKALVMKLNKELLGAKIVLSNPYYILM 585

Query: 1051 HIVKKPSLFGFDVAAVACCSSGLFEMSYLCDRMNPFTCTDADKYVFWDSFHPTQKTNQIV 1230
            ++VK+PS+FGF+ AAVACCS+G+FEM Y C R+NPFTC DADKYVFWD+FHPTQKTN I+
Sbjct: 586  NMVKRPSVFGFENAAVACCSTGMFEMGYACSRLNPFTCNDADKYVFWDAFHPTQKTNSII 645

Query: 1231 SEHLIKNVLHVFL 1269
            + +++K VL  FL
Sbjct: 646  AYYVVKKVLAQFL 658



 Score =  441 bits (1134), Expect = e-121
 Identities = 208/297 (70%), Positives = 242/297 (81%)
 Frame = +1

Query: 331  GNNNQISTVLKSNFEPYGRDFYGGKPTGRFSNGKIPPDFISEAFGLKPTVPAYLDPAYNI 510
            GNNNQISTVLKSNF PYGRDF GG+PTGRFSNG+IPPDFISEAFGLKPTVPAYLDP YNI
Sbjct: 39   GNNNQISTVLKSNFVPYGRDFTGGRPTGRFSNGRIPPDFISEAFGLKPTVPAYLDPNYNI 98

Query: 511  SDFVDGVNFASAGTGYDTATSDVLSVIPFWKELDYYKEYQSKLKAYVGDAKANEITSEAL 690
            SDF  GV FASAGTGYD  TSDVLSVIP WKEL+YYKEYQ KL+AY+G  KANEI SE+L
Sbjct: 99   SDFATGVCFASAGTGYDNQTSDVLSVIPLWKELEYYKEYQKKLRAYLGQEKANEILSESL 158

Query: 691  YLLSLGTNDFLENYYTVPTRRDQFTVDKYQDYLCGIADKFIRELYALGAKKISFGGLPPM 870
            YL+SLGTNDFLENYY    R  Q+TV +Y+D+L GIA  FI+E+Y+LGA+K+S GGLPPM
Sbjct: 159  YLMSLGTNDFLENYYIFSGRSSQYTVPQYEDFLVGIAGNFIKEIYSLGARKVSLGGLPPM 218

Query: 871  GCLPLERTADFFRGNGDVCNEDYNKVALDFXXXXXXXXXXXXQELSGLRLVLSNPYFILQ 1050
            GCLPLERT +FF   G  C E YN VA++F            ++L G+++VLSNPYFILQ
Sbjct: 219  GCLPLERTTNFF--GGSECIERYNNVAMEFNGKLNTLVGKLNKQLPGIKVVLSNPYFILQ 276

Query: 1051 HIVKKPSLFGFDVAAVACCSSGLFEMSYLCDRMNPFTCTDADKYVFWDSFHPTQKTN 1221
             I++KPS +G++ AAVACC++G+FEM YLC+R N  TC DA KYVFWDSFHPT+KTN
Sbjct: 277  KIIRKPSSYGYENAAVACCATGMFEMGYLCNRYNMLTCPDASKYVFWDSFHPTEKTN 333


>ref|XP_002276681.1| PREDICTED: GDSL esterase/lipase At2g04570 [Vitis vinifera]
          Length = 349

 Score =  454 bits (1168), Expect = e-125
 Identities = 211/313 (67%), Positives = 255/313 (81%)
 Frame = +1

Query: 331  GNNNQISTVLKSNFEPYGRDFYGGKPTGRFSNGKIPPDFISEAFGLKPTVPAYLDPAYNI 510
            GNNNQIST+ KSNFEPYGR+F GG+PTGRFSNG+I  DFISEAFGLKPTVPAYLDP Y+I
Sbjct: 39   GNNNQISTIAKSNFEPYGRNFPGGRPTGRFSNGRISTDFISEAFGLKPTVPAYLDPTYSI 98

Query: 511  SDFVDGVNFASAGTGYDTATSDVLSVIPFWKELDYYKEYQSKLKAYVGDAKANEITSEAL 690
             DF  GV+FASAG+GYD ATSDVLSVIP WKEL+YYK+YQ++L+AY+G  KANE+ SEAL
Sbjct: 99   KDFATGVSFASAGSGYDNATSDVLSVIPLWKELEYYKDYQTELRAYLGVKKANEVLSEAL 158

Query: 691  YLLSLGTNDFLENYYTVPTRRDQFTVDKYQDYLCGIADKFIRELYALGAKKISFGGLPPM 870
            Y++SLGTNDFLENYY  P R  QFT+ +Y+D+L GIA  F+ +LY LGA+KIS GGLPPM
Sbjct: 159  YVMSLGTNDFLENYYAFPNRSSQFTIKQYEDFLIGIAGHFVHQLYGLGARKISVGGLPPM 218

Query: 871  GCLPLERTADFFRGNGDVCNEDYNKVALDFXXXXXXXXXXXXQELSGLRLVLSNPYFILQ 1050
            GC+PLERT +F   NG  C E+YN VALDF            +EL G ++VLSNPY+IL 
Sbjct: 219  GCMPLERTTNFM--NGAECVEEYNNVALDFNWKLKALVMKLNKELLGAKIVLSNPYYILM 276

Query: 1051 HIVKKPSLFGFDVAAVACCSSGLFEMSYLCDRMNPFTCTDADKYVFWDSFHPTQKTNQIV 1230
            ++VK+PS+FGF+ AAVACCS+G+FEM Y C R+NPFTC DADKYVFWD+FHPTQKTN I+
Sbjct: 277  NMVKRPSVFGFENAAVACCSTGMFEMGYACSRLNPFTCNDADKYVFWDAFHPTQKTNSII 336

Query: 1231 SEHLIKNVLHVFL 1269
            + +++K VL  FL
Sbjct: 337  AYYVVKKVLAQFL 349


>ref|XP_002271851.1| PREDICTED: GDSL esterase/lipase At2g04570-like [Vitis vinifera]
          Length = 342

 Score =  439 bits (1130), Expect = e-121
 Identities = 211/313 (67%), Positives = 249/313 (79%)
 Frame = +1

Query: 331  GNNNQISTVLKSNFEPYGRDFYGGKPTGRFSNGKIPPDFISEAFGLKPTVPAYLDPAYNI 510
            GNNNQISTVLKSNF PYGRDF GG+PTGRFSNG+IPPDFISEAFGLKPTVPAYLDP YNI
Sbjct: 39   GNNNQISTVLKSNFVPYGRDFTGGRPTGRFSNGRIPPDFISEAFGLKPTVPAYLDPNYNI 98

Query: 511  SDFVDGVNFASAGTGYDTATSDVLSVIPFWKELDYYKEYQSKLKAYVGDAKANEITSEAL 690
            SDF  GV FASAGTGYD  TSDVL       EL+YYKEYQ KL+AY+G  KANEI SE+L
Sbjct: 99   SDFATGVCFASAGTGYDNQTSDVL-------ELEYYKEYQKKLRAYLGQEKANEILSESL 151

Query: 691  YLLSLGTNDFLENYYTVPTRRDQFTVDKYQDYLCGIADKFIRELYALGAKKISFGGLPPM 870
            YL+SLGTNDFLENYY    R  Q+TV +Y+D+L GIA  FI+E+Y+LGA+K+S GGLPPM
Sbjct: 152  YLMSLGTNDFLENYYIFSGRSSQYTVPQYEDFLVGIAGNFIKEIYSLGARKVSLGGLPPM 211

Query: 871  GCLPLERTADFFRGNGDVCNEDYNKVALDFXXXXXXXXXXXXQELSGLRLVLSNPYFILQ 1050
            GCLPLERT +FF   G  C E YN VA++F            ++L G+++VLSNPYFILQ
Sbjct: 212  GCLPLERTTNFF--GGSECIERYNNVAMEFNGKLNTLVGKLNKQLPGIKVVLSNPYFILQ 269

Query: 1051 HIVKKPSLFGFDVAAVACCSSGLFEMSYLCDRMNPFTCTDADKYVFWDSFHPTQKTNQIV 1230
             I++KPS +G++ AAVACC++G+FEM YLC+R N  TC DA KYVFWDSFHPT+KTN I+
Sbjct: 270  KIIRKPSSYGYENAAVACCATGMFEMGYLCNRYNMLTCPDASKYVFWDSFHPTEKTNGII 329

Query: 1231 SEHLIKNVLHVFL 1269
            S+H++K VL VFL
Sbjct: 330  SDHVVKTVLKVFL 342


>ref|XP_004140128.1| PREDICTED: GDSL esterase/lipase At2g04570-like [Cucumis sativus]
            gi|449481120|ref|XP_004156087.1| PREDICTED: GDSL
            esterase/lipase At2g04570-like [Cucumis sativus]
          Length = 354

 Score =  436 bits (1122), Expect = e-120
 Identities = 205/313 (65%), Positives = 247/313 (78%)
 Frame = +1

Query: 331  GNNNQISTVLKSNFEPYGRDFYGGKPTGRFSNGKIPPDFISEAFGLKPTVPAYLDPAYNI 510
            GNNN I T+ +SNF PYGRDF GGK TGRFSNG+IP DFISEAFGLKPT+PAYLDPAY I
Sbjct: 44   GNNNFIPTIARSNFFPYGRDFTGGKATGRFSNGRIPTDFISEAFGLKPTIPAYLDPAYTI 103

Query: 511  SDFVDGVNFASAGTGYDTATSDVLSVIPFWKELDYYKEYQSKLKAYVGDAKANEITSEAL 690
            SD   G+ FASAGTGYD ATS+VLSVIP WK+L+YYKEYQ+KL AY G + ANE   EAL
Sbjct: 104  SDLATGLTFASAGTGYDNATSNVLSVIPLWKQLEYYKEYQAKLIAYQGSSTANETIKEAL 163

Query: 691  YLLSLGTNDFLENYYTVPTRRDQFTVDKYQDYLCGIADKFIRELYALGAKKISFGGLPPM 870
            Y++SLGTNDFLENYYT+P R  Q+ + +YQD+L GIA  FI +LY+LGA+KIS GGLPPM
Sbjct: 164  YVMSLGTNDFLENYYTMPGRSSQYNIQQYQDFLVGIASGFIEKLYSLGARKISLGGLPPM 223

Query: 871  GCLPLERTADFFRGNGDVCNEDYNKVALDFXXXXXXXXXXXXQELSGLRLVLSNPYFILQ 1050
            GCLPLERT + F GN   C E YN VA+DF            ++L G++LV SNPY +L 
Sbjct: 224  GCLPLERTRNLFGGNN--CLESYNNVAVDFNNKLKALTVKLNKDLPGIQLVFSNPYDVLL 281

Query: 1051 HIVKKPSLFGFDVAAVACCSSGLFEMSYLCDRMNPFTCTDADKYVFWDSFHPTQKTNQIV 1230
             ++KKPSL+GFDV + ACC++G+FEM Y C+R + FTCTDA+KY+FWDSFHPTQKTNQ+V
Sbjct: 282  SMIKKPSLYGFDVTSTACCATGMFEMGYACNRDSMFTCTDANKYIFWDSFHPTQKTNQLV 341

Query: 1231 SEHLIKNVLHVFL 1269
            S +++KNVL  FL
Sbjct: 342  SSYVVKNVLSQFL 354