BLASTX nr result
ID: Bupleurum21_contig00018722
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00018722
(2003 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002276511.1| PREDICTED: uncharacterized RNA methyltransfe... 814 0.0
ref|XP_003597643.1| 23S rRNA (uracil-5-)-methyltransferase rumA ... 796 0.0
ref|XP_002515896.1| RNA m5u methyltransferase, putative [Ricinus... 784 0.0
ref|XP_002885434.1| RNA methyltransferase family protein [Arabid... 778 0.0
ref|NP_188767.2| RNA methyltransferase family protein [Arabidops... 777 0.0
>ref|XP_002276511.1| PREDICTED: uncharacterized RNA methyltransferase CT0009 [Vitis
vinifera] gi|297743126|emb|CBI35993.3| unnamed protein
product [Vitis vinifera]
Length = 570
Score = 814 bits (2103), Expect = 0.0
Identities = 402/564 (71%), Positives = 464/564 (82%), Gaps = 2/564 (0%)
Frame = -3
Query: 1962 PQKMATAAALPSFRLRRLNTYYNPWLLNFRXXXXXXXXXXXXXXXXXXXSEETQVSTATT 1783
P MAT +P++ LR LN+ PW+ NFR EE+Q +
Sbjct: 26 PPPMAT---VPTYGLRWLNSG-RPWMQNFRRSSILSSAIPAIQDS-----EESQFDSP-- 74
Query: 1782 SNPRNEDNKKKATSFYPKRGQTLELECESIAYKGKGVCKVADTGFVLMCDRVLPGERFIG 1603
K K S++PKRGQTLEL CES+A+KGKG+CKVADTGFV++CDR LPGERF+G
Sbjct: 75 --------KPKTQSYFPKRGQTLELVCESLAFKGKGLCKVADTGFVVLCDRALPGERFVG 126
Query: 1602 RVTRKKDNYAEATKLKTLSPHRDAVEAPCEYALHCGGCKTQNLLYEAQVKAKEQQVHELV 1423
RVTRKK NYAE TK++T+SPH D V+APCEYA +CGGCKTQNL YEAQV+AKEQQV ELV
Sbjct: 127 RVTRKKGNYAEVTKVETISPHWDIVDAPCEYASNCGGCKTQNLSYEAQVRAKEQQVQELV 186
Query: 1422 VHVGRFSHK--ELDGIMKPIVPCDIQFHYRNKMEFSFGPKKWVPKELLEEARSNNSNDYA 1249
+HVG+FS K E IMKPIVPCD+QFHYRNKMEFSF +KW+P+ELL E + + ++ YA
Sbjct: 187 IHVGKFSDKDPEFSSIMKPIVPCDVQFHYRNKMEFSFATQKWLPRELLHE-KEDGASGYA 245
Query: 1248 LGLHVPGFFDKVLNVDRCLIQSEPANKVLAAIQDIWRESQLGLSPYNCHTHAGFLKHLML 1069
LGLH PGFFDKVLNV++CL+QSEPAN VLAAIQ+ WR+ Q GLSPYN H+H GFLKHL+L
Sbjct: 246 LGLHAPGFFDKVLNVNKCLLQSEPANTVLAAIQECWRDPQFGLSPYNVHSHDGFLKHLVL 305
Query: 1068 RTGRDVKTGLPELMVNFVTASYEPDLLKPLVEKIKAIPEVVSIMXXXXXXXXXXXXGEKE 889
RTGRD+KTGLPELMVNFVT+SY+P+LLKPLVEKI AIPEVVSI+ GE+E
Sbjct: 306 RTGRDIKTGLPELMVNFVTSSYKPELLKPLVEKISAIPEVVSIVNNINTSVGSTSVGEQE 365
Query: 888 YTLYGKPTITEILRGLTFQISANSFFQTNTLQAEVLYKLIEDCATLKGDGSEVVLDLFCG 709
Y L+GK ITEILRGLTFQISANSFFQTNT QAEVLYKLIEDCA L+GDGSE+VLDLFCG
Sbjct: 366 YILHGKSAITEILRGLTFQISANSFFQTNTHQAEVLYKLIEDCAGLRGDGSEIVLDLFCG 425
Query: 708 TGTIGLTLAKRVRHIYGYEVVPQAISDARRNAKLNGIDNATFVQGDLNKIGENFGKEFPK 529
TGTIGLTL++RVRH+YGYEVV QA+SDARRNAKLNGI NATF++GDLNKIGENFGK+FPK
Sbjct: 426 TGTIGLTLSRRVRHVYGYEVVAQAVSDARRNAKLNGICNATFIEGDLNKIGENFGKDFPK 485
Query: 528 PDIVITDPNRPGMHLKLIIFLLKLRAANIIYVSCNPSTCARDLDYLCHGVAEKNIEGCYK 349
PD+VI+DPNRPGMH+KLI FLLKL+A I+YVSCNP+TCARDLDYLCHGV E+NIEGCYK
Sbjct: 486 PDVVISDPNRPGMHMKLIKFLLKLKAPRIVYVSCNPATCARDLDYLCHGVVEQNIEGCYK 545
Query: 348 LRSLQPVDMFPHTPHIECVCLLEL 277
L+SLQPVDMFPHTPHIECVCLLEL
Sbjct: 546 LKSLQPVDMFPHTPHIECVCLLEL 569
>ref|XP_003597643.1| 23S rRNA (uracil-5-)-methyltransferase rumA [Medicago truncatula]
gi|355486691|gb|AES67894.1| 23S rRNA
(uracil-5-)-methyltransferase rumA [Medicago truncatula]
Length = 560
Score = 796 bits (2055), Expect = 0.0
Identities = 383/515 (74%), Positives = 439/515 (85%), Gaps = 3/515 (0%)
Frame = -3
Query: 1809 ETQVSTATTSNPRNEDNKKKATSFYPKRGQTLELECESIAYKGKGVCKVADTGFVLMCDR 1630
E+Q TT++ N +TS++PKRGQTLEL CES+A+KGKG+CKV DTGFV+MCDR
Sbjct: 46 ESQPEPTTTTSIPKTPNPNTSTSYFPKRGQTLELLCESLAFKGKGLCKVTDTGFVVMCDR 105
Query: 1629 VLPGERFIGRVTRKKDNYAEATKLKTLSPHRDAVEAPCEYALHCGGCKTQNLLYEAQVKA 1450
LPGERFIGR+TRKK NYAEATK++TL+P V+APC Y HCGGCKTQNL Y AQV A
Sbjct: 106 ALPGERFIGRITRKKGNYAEATKVETLTPPFHVVDAPCVYVPHCGGCKTQNLAYLAQVNA 165
Query: 1449 KEQQVHELVVHVGRFSHKELD--GIMKPIVPCDIQFHYRNKMEFSFGPKKWVPKELLEEA 1276
KE+QV +LV+HVGRFS KEL+ GIMKPIVPCDIQFHYRNKMEFSFGP KW+PKE L E
Sbjct: 166 KEEQVRDLVIHVGRFSQKELELHGIMKPIVPCDIQFHYRNKMEFSFGPYKWLPKESLHER 225
Query: 1275 RSN-NSNDYALGLHVPGFFDKVLNVDRCLIQSEPANKVLAAIQDIWRESQLGLSPYNCHT 1099
+ S +Y+LGLHVPGFFDK++NV++CL+Q++PANKVLAAIQ+ WR+ QLG SPYN H+
Sbjct: 226 NVDAGSENYSLGLHVPGFFDKIINVEKCLLQTDPANKVLAAIQECWRDPQLGFSPYNVHS 285
Query: 1098 HAGFLKHLMLRTGRDVKTGLPELMVNFVTASYEPDLLKPLVEKIKAIPEVVSIMXXXXXX 919
H GFLKHLMLR+GRDV TGLPE+MVNFVT+SY+PDLLK LV+K+ A PEVVSIM
Sbjct: 286 HKGFLKHLMLRSGRDVMTGLPEVMVNFVTSSYKPDLLKVLVDKVSAFPEVVSIMNNVNTS 345
Query: 918 XXXXXXGEKEYTLYGKPTITEILRGLTFQISANSFFQTNTLQAEVLYKLIEDCATLKGDG 739
GE+EYTL+GK +ITE LRGLTFQISANSFFQTNT QAEVLYKLIE+CA ++GDG
Sbjct: 346 VGNTSVGEEEYTLHGKSSITETLRGLTFQISANSFFQTNTHQAEVLYKLIEECAGIRGDG 405
Query: 738 SEVVLDLFCGTGTIGLTLAKRVRHIYGYEVVPQAISDARRNAKLNGIDNATFVQGDLNKI 559
SEVVLDLFCGTGTIGLTLA+ V+H+YGYEVVPQAI+DAR NAK+NGI NATF++GDLNKI
Sbjct: 406 SEVVLDLFCGTGTIGLTLARSVKHVYGYEVVPQAIADARLNAKINGIQNATFIEGDLNKI 465
Query: 558 GENFGKEFPKPDIVITDPNRPGMHLKLIIFLLKLRAANIIYVSCNPSTCARDLDYLCHGV 379
NFGK FPKPDIVI+DPNRPGMH+KLI FLL LRA I+YVSCNP+TCARDLDYLCHGV
Sbjct: 466 DANFGKNFPKPDIVISDPNRPGMHMKLIKFLLNLRAPRIVYVSCNPATCARDLDYLCHGV 525
Query: 378 AEKNIEGCYKLRSLQPVDMFPHTPHIECVCLLELC 274
AE NIEGCYKL SLQPVDMFPHTPHIECVCLLELC
Sbjct: 526 AELNIEGCYKLISLQPVDMFPHTPHIECVCLLELC 560
>ref|XP_002515896.1| RNA m5u methyltransferase, putative [Ricinus communis]
gi|223544801|gb|EEF46316.1| RNA m5u methyltransferase,
putative [Ricinus communis]
Length = 577
Score = 784 bits (2025), Expect = 0.0
Identities = 380/515 (73%), Positives = 438/515 (85%), Gaps = 12/515 (2%)
Frame = -3
Query: 1782 SNPRNEDNKK----------KATSFYPKRGQTLELECESIAYKGKGVCKVADTGFVLMCD 1633
+N + NKK K+ + +PKRGQ+LEL CES+A+KGKGVCKVADTGFV+MCD
Sbjct: 64 NNKKKNKNKKDSIELNADSSKSGALFPKRGQSLELVCESLAFKGKGVCKVADTGFVVMCD 123
Query: 1632 RVLPGERFIGRVTRKKDNYAEATKLKTLSPHRDAVEAPCEYALHCGGCKTQNLLYEAQVK 1453
R LPGERFIGRVTR+K +YAE TK+KT+SPH D +EAPCEYA +CGGCKTQNL YEAQV+
Sbjct: 124 RALPGERFIGRVTRRKGSYAEVTKVKTISPHWDIIEAPCEYASYCGGCKTQNLSYEAQVR 183
Query: 1452 AKEQQVHELVVHVGRFSH--KELDGIMKPIVPCDIQFHYRNKMEFSFGPKKWVPKELLEE 1279
AKE QV ELV+++G+FS E IMKPIVPCD++FHYRNKMEFSFG KKW+P+ELL E
Sbjct: 184 AKELQVRELVINIGKFSDIDPEFVSIMKPIVPCDVKFHYRNKMEFSFGSKKWLPRELLGE 243
Query: 1278 ARSNNSNDYALGLHVPGFFDKVLNVDRCLIQSEPANKVLAAIQDIWRESQLGLSPYNCHT 1099
+ + N YALGLH PG FDKVLNVD+CL+QSEPAN VLA +QD WR+SQ GL+PY+ H+
Sbjct: 244 KQDGSVN-YALGLHAPGCFDKVLNVDKCLLQSEPANTVLATVQDCWRDSQFGLTPYDVHS 302
Query: 1098 HAGFLKHLMLRTGRDVKTGLPELMVNFVTASYEPDLLKPLVEKIKAIPEVVSIMXXXXXX 919
H GFLKHLMLRTGR+ +TGL ELMVNFVT++Y+P+LLK +VEKI AIPEVVSI+
Sbjct: 303 HDGFLKHLMLRTGRNTETGLLELMVNFVTSAYKPELLKIIVEKISAIPEVVSIVNNVNSS 362
Query: 918 XXXXXXGEKEYTLYGKPTITEILRGLTFQISANSFFQTNTLQAEVLYKLIEDCATLKGDG 739
GE+EYT+YGK TITEILRGLTFQISANSFFQTNT QAEVLYKLIEDCA L+GDG
Sbjct: 363 VGNTSVGEEEYTIYGKSTITEILRGLTFQISANSFFQTNTRQAEVLYKLIEDCAGLRGDG 422
Query: 738 SEVVLDLFCGTGTIGLTLAKRVRHIYGYEVVPQAISDARRNAKLNGIDNATFVQGDLNKI 559
SE+VLDLFCGTGTIGLTLA R +H+YGYEVVPQAISDARRNA+LNGI NATFVQGDLNKI
Sbjct: 423 SEIVLDLFCGTGTIGLTLAGRAKHVYGYEVVPQAISDARRNAELNGITNATFVQGDLNKI 482
Query: 558 GENFGKEFPKPDIVITDPNRPGMHLKLIIFLLKLRAANIIYVSCNPSTCARDLDYLCHGV 379
++FG FPKPDIVI+DPNRPGMH+KLI FLLKL+A I+YVSCNP+TCARDLDYLCHGV
Sbjct: 483 SDSFGNNFPKPDIVISDPNRPGMHMKLIKFLLKLKAPRIVYVSCNPATCARDLDYLCHGV 542
Query: 378 AEKNIEGCYKLRSLQPVDMFPHTPHIECVCLLELC 274
E+NI+GCYKL+SLQPVDMFPHTPHIECVCLLELC
Sbjct: 543 EEQNIKGCYKLKSLQPVDMFPHTPHIECVCLLELC 577
>ref|XP_002885434.1| RNA methyltransferase family protein [Arabidopsis lyrata subsp.
lyrata] gi|297331274|gb|EFH61693.1| RNA methyltransferase
family protein [Arabidopsis lyrata subsp. lyrata]
Length = 559
Score = 778 bits (2008), Expect = 0.0
Identities = 372/509 (73%), Positives = 432/509 (84%), Gaps = 6/509 (1%)
Frame = -3
Query: 1785 TSNPRNEDN----KKKATSFYPKRGQTLELECESIAYKGKGVCKVADTGFVLMCDRVLPG 1618
T N++N +K +YPKRGQT+EL CES+ +KGKG+CKV TGFV+MCDR LPG
Sbjct: 44 TQKKLNDENINGSNEKTAPYYPKRGQTVELVCESLGFKGKGICKVDGTGFVVMCDRALPG 103
Query: 1617 ERFIGRVTRKKDNYAEATKLKTLSPHRDAVEAPCEYALHCGGCKTQNLLYEAQVKAKEQQ 1438
ERF+GR+TR+K +YAE TK+KT+SPH+D VEAPCEYA +CGGCKTQNL YEAQ+KAKE+Q
Sbjct: 104 ERFLGRITRRKGSYAEVTKIKTISPHKDLVEAPCEYASYCGGCKTQNLSYEAQLKAKEEQ 163
Query: 1437 VHELVVHVGRFSHKE--LDGIMKPIVPCDIQFHYRNKMEFSFGPKKWVPKELLEEARSNN 1264
VHEL+ HVGRFS L+ ++KPIV CDIQF+YRNKMEFSFGP++W+P E+L E
Sbjct: 164 VHELIRHVGRFSDNNPGLEIVLKPIVACDIQFNYRNKMEFSFGPQRWLPIEMLHEKEDGP 223
Query: 1263 SNDYALGLHVPGFFDKVLNVDRCLIQSEPANKVLAAIQDIWRESQLGLSPYNCHTHAGFL 1084
N +ALGLH PGFFDKVLNVD+CL+QSEP N VLAA+QD WR+ L LSPYNC +H GFL
Sbjct: 224 KN-FALGLHAPGFFDKVLNVDKCLLQSEPGNLVLAAVQDCWRDPALSLSPYNCRSHVGFL 282
Query: 1083 KHLMLRTGRDVKTGLPELMVNFVTASYEPDLLKPLVEKIKAIPEVVSIMXXXXXXXXXXX 904
KHLMLRTGR+V+TG ELMVNFVT+SYEP+LLKPLV++I +IP+VVSIM
Sbjct: 283 KHLMLRTGRNVETGSLELMVNFVTSSYEPELLKPLVDRISSIPQVVSIMNNVNSSVGNTS 342
Query: 903 XGEKEYTLYGKPTITEILRGLTFQISANSFFQTNTLQAEVLYKLIEDCATLKGDGSEVVL 724
GEKEYTLYGK TITE+LRGLTFQISANSFFQTNT QAEVLYKLIE+ A LKGDGSEVVL
Sbjct: 343 VGEKEYTLYGKDTITEVLRGLTFQISANSFFQTNTHQAEVLYKLIEESAGLKGDGSEVVL 402
Query: 723 DLFCGTGTIGLTLAKRVRHIYGYEVVPQAISDARRNAKLNGIDNATFVQGDLNKIGENFG 544
DLFCGTGTIGLTLA+R +HIYGYEVVPQAI+DA +NA++NGI+NATF+QGDLNKIGE+FG
Sbjct: 403 DLFCGTGTIGLTLARRAKHIYGYEVVPQAITDAHKNAQINGIENATFIQGDLNKIGEDFG 462
Query: 543 KEFPKPDIVITDPNRPGMHLKLIIFLLKLRAANIIYVSCNPSTCARDLDYLCHGVAEKNI 364
FPKPDIVI+DPNRPGMH+KLI FLLKL++ IIYVSCNP+TCARDLDYLCHGV EKNI
Sbjct: 463 NNFPKPDIVISDPNRPGMHMKLIKFLLKLKSPRIIYVSCNPATCARDLDYLCHGVEEKNI 522
Query: 363 EGCYKLRSLQPVDMFPHTPHIECVCLLEL 277
+GCYKL S+QPVDMFPHTPHIECVC LEL
Sbjct: 523 KGCYKLMSVQPVDMFPHTPHIECVCFLEL 551
>ref|NP_188767.2| RNA methyltransferase family protein [Arabidopsis thaliana]
gi|9280234|dbj|BAB01724.1| RNA methyltransferase-like
protein [Arabidopsis thaliana]
gi|332642969|gb|AEE76490.1| RNA methyltransferase family
protein [Arabidopsis thaliana]
Length = 554
Score = 777 bits (2007), Expect = 0.0
Identities = 377/557 (67%), Positives = 451/557 (80%), Gaps = 2/557 (0%)
Frame = -3
Query: 1941 AALPSFRLRRLNTYYNPWLLNFRXXXXXXXXXXXXXXXXXXXSEETQVSTATTSNPRNED 1762
AA P+ LR + WL FR +++ +++ +N NE
Sbjct: 3 AATPAHGLRYVVVPTRSWLHRFRCCSSSLAPLSPSEFSGGNKAQK-KLNDENNTNVSNE- 60
Query: 1761 NKKKATSFYPKRGQTLELECESIAYKGKGVCKVADTGFVLMCDRVLPGERFIGRVTRKKD 1582
K +YPKRG+T+EL CES+ +KGKG+CKV TG+V+MCDR LPGERF+GRVTR+K
Sbjct: 61 ---KTAPYYPKRGETVELVCESLGFKGKGICKVDGTGYVVMCDRALPGERFLGRVTRRKG 117
Query: 1581 NYAEATKLKTLSPHRDAVEAPCEYALHCGGCKTQNLLYEAQVKAKEQQVHELVVHVGRFS 1402
+YAE TK+KT+SPH+D VEAPCEYA +CGGCKTQNL YEAQ++AKE+QVHEL+ HVGRFS
Sbjct: 118 SYAEVTKMKTISPHKDLVEAPCEYASYCGGCKTQNLSYEAQLRAKEEQVHELIRHVGRFS 177
Query: 1401 HKE--LDGIMKPIVPCDIQFHYRNKMEFSFGPKKWVPKELLEEARSNNSNDYALGLHVPG 1228
L+ ++KPIV CDIQFHYRNKMEFSFGP++W+P E+L E R + ++ALGLH PG
Sbjct: 178 DNNPGLEIVLKPIVACDIQFHYRNKMEFSFGPQRWLPIEMLNE-RQDGPKNFALGLHAPG 236
Query: 1227 FFDKVLNVDRCLIQSEPANKVLAAIQDIWRESQLGLSPYNCHTHAGFLKHLMLRTGRDVK 1048
FFDKVLNVD+CL+QSEP N VLAA+QD WR+ +L LSPY+C +H GFLKHLMLRTGR+V+
Sbjct: 237 FFDKVLNVDKCLLQSEPGNLVLAAVQDCWRDPELSLSPYDCRSHVGFLKHLMLRTGRNVE 296
Query: 1047 TGLPELMVNFVTASYEPDLLKPLVEKIKAIPEVVSIMXXXXXXXXXXXXGEKEYTLYGKP 868
TG ELMVNFVT+SY+P+LLKPLV++I +IP+VVSIM GEKEYTLYGK
Sbjct: 297 TGSLELMVNFVTSSYKPELLKPLVDRISSIPQVVSIMNNVNSSVGNTSVGEKEYTLYGKD 356
Query: 867 TITEILRGLTFQISANSFFQTNTLQAEVLYKLIEDCATLKGDGSEVVLDLFCGTGTIGLT 688
TITE+LRGLTFQISANSFFQTNT QAEVLYKLIE+ A LKGDGSEVVLDLFCGTGTIGLT
Sbjct: 357 TITEVLRGLTFQISANSFFQTNTHQAEVLYKLIEESAGLKGDGSEVVLDLFCGTGTIGLT 416
Query: 687 LAKRVRHIYGYEVVPQAISDARRNAKLNGIDNATFVQGDLNKIGENFGKEFPKPDIVITD 508
LA+R +H+YGYEVVPQAI+DA +NA++NGI+NATF+QGDLNKIGE+FG FPKPDIVI+D
Sbjct: 417 LARRAKHVYGYEVVPQAITDAHKNAQINGIENATFIQGDLNKIGEDFGNNFPKPDIVISD 476
Query: 507 PNRPGMHLKLIIFLLKLRAANIIYVSCNPSTCARDLDYLCHGVAEKNIEGCYKLRSLQPV 328
PNRPGMH+KLI FLLKL++ IIYVSCNP+TCARDLDYLCHGV EKN++GCYKL S+QPV
Sbjct: 477 PNRPGMHMKLIKFLLKLKSPRIIYVSCNPATCARDLDYLCHGVEEKNLKGCYKLMSVQPV 536
Query: 327 DMFPHTPHIECVCLLEL 277
DMFPHTPHIECVCLLEL
Sbjct: 537 DMFPHTPHIECVCLLEL 553