BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00018627
         (1172 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003601344.1| Double-stranded RNA binding protein [Medicag...   221   2e-55
ref|XP_003592245.1| Double-stranded RNA binding protein [Medicag...   217   5e-54
ref|XP_002319709.1| predicted protein [Populus trichocarpa] gi|2...   211   4e-52
ref|XP_002325855.1| predicted protein [Populus trichocarpa] gi|2...   190   7e-46
ref|XP_003592251.1| Double-stranded RNA binding protein [Medicag...   168   2e-39

>ref|XP_003601344.1| Double-stranded RNA binding protein [Medicago truncatula]
            gi|355490392|gb|AES71595.1| Double-stranded RNA binding
            protein [Medicago truncatula]
          Length = 372

 Score =  221 bits (564), Expect = 2e-55
 Identities = 145/383 (37%), Positives = 201/383 (52%), Gaps = 19/383 (4%)
 Frame = -3

Query: 1155 MFKSKLQEICHQQKWGLPKYN-FIKEGPDHNPSFKASVTVNDVTFDTTIYSKSSKEAQNS 979
            M KSKLQE+CH+++W LPKY+ F  +GP HNPSFK SV VN +TF ++    SS EAQN 
Sbjct: 1    MHKSKLQELCHRRRWSLPKYSAFHVDGPPHNPSFKGSVFVNGLTFTSSDTFNSSGEAQNQ 60

Query: 978  AAELAVLHFTA-----SEPRAEPKTGDPVIEIHQAEE-MHSDSSVFKNDELNSYKMMLQT 817
            AA  A  +FT+     S+P  E  + + V  +   E  +   S V K+D  +      Q 
Sbjct: 61   AAMKAFRNFTSPLSSSSKPTNEHGSKEEVKAVKPQESPVLQQSPVIKSDTDH------QN 114

Query: 816  YTQRKKVGMPVYTSVKTGLPHALCFKATVIVAGHPFESPRSYKTLKEAEHAAAQVALLSF 637
              ++  +  PV+T    G PH + +KA V++ G  FESP S+ T+KEAE AAA+   +  
Sbjct: 115  CARKNDLDQPVFTIKTEGPPHDIRYKAIVVIDGKSFESPTSFNTIKEAEQAAAKFVGM-- 172

Query: 636  TLDDFQQIGSPLYKNMLQELAQNEGFTLPKYKTEHCFELHYPSFISTVEVEGEIFHGAAA 457
                FQ+      K++LQEL++ EGF+ P YKT      H P+F STVEVEG  FHG A+
Sbjct: 173  ----FQKDEPCPSKSLLQELSEREGFSKPTYKTTQIGPPHMPTFFSTVEVEGIGFHGKAS 228

Query: 456  RSKKQAESNAAKVAYTAFMERKRSRIANXXXXXSNYEA------TEVIPGSVSSSPINLH 295
            +SK +AE +AAK+AY    E      A+        +A      + ++       P+   
Sbjct: 229  KSKNKAEEDAAKIAYITLKECGSHTYADLSSSIGEKQAVKSTHESHIVKSKPKLKPVPED 288

Query: 294  GDFKSDCSAVSTQCANHSGQSFQEKCPNLK------ESSKTECYLLCNRFKVYTSIPDID 133
                 +      Q  N          PN K      ++  T  Y+L N+ KVYTS PDI 
Sbjct: 289  ELLYDEILPTDFQVNNGIQNESLPLPPNKKMKISKAKTPNTNSYMLGNKLKVYTSFPDIV 348

Query: 132  FPKGTCLLPIAEDKWIVVSLECP 64
            FP+G  ++PI EDKWI  SLE P
Sbjct: 349  FPEGITVVPIGEDKWIAASLEFP 371


>ref|XP_003592245.1| Double-stranded RNA binding protein [Medicago truncatula]
            gi|357462121|ref|XP_003601342.1| Double-stranded RNA
            binding protein [Medicago truncatula]
            gi|355481293|gb|AES62496.1| Double-stranded RNA binding
            protein [Medicago truncatula] gi|355490390|gb|AES71593.1|
            Double-stranded RNA binding protein [Medicago truncatula]
          Length = 424

 Score =  217 bits (552), Expect = 5e-54
 Identities = 152/420 (36%), Positives = 208/420 (49%), Gaps = 56/420 (13%)
 Frame = -3

Query: 1155 MFKSKLQEICHQQKWGLPKYNFIKEGPDHNPSFKASVTVNDVTFDTTIYSKSSKEAQNSA 976
            MFK+KLQ++CHQ++W LPKY    +GP H PSF ASV VN VTF ++    S+KEAQN A
Sbjct: 1    MFKTKLQQLCHQRRWSLPKYTATNDGPQHKPSFNASVHVNGVTFASSDAFNSAKEAQNQA 60

Query: 975  AELAVLHFTA---------------SEPRAEPKTGDPVIEIH-QAEEMHSDSSVFKNDEL 844
            A  A  +FT+               S P  E  + + V     Q   + + S V + D  
Sbjct: 61   AMKAFRNFTSPLSEFAIRLIRRDGFSIPTDEYGSKEKVEATKPQESPLPAQSPVIRTDTN 120

Query: 843  NSYKMMLQTYTQRKKVGMPVYTSVKTGLPHALCFKATVIVAGHPFESPRSYKTLKEAEHA 664
               K  LQ Y ++  +  PV+T +KT   H   +KATV++    FESP  + ++KEAE A
Sbjct: 121  RLSKKHLQNYARKNNLDPPVFT-IKTERLH---YKATVVIDEKSFESPTFFNSIKEAEQA 176

Query: 663  AAQVAL--LSFTLDDFQQIGSPLYKNMLQELAQNEGFTLPKYKTEHCFELHYPSFISTVE 490
            AA++AL  L  ++D FQ+  S   K++L EL Q EG++ P Y T     LH  ++ STVE
Sbjct: 177  AAKIALRELPISVDLFQKDESCPSKSLLLELTQREGYSKPTYTTIESGSLHMRTYFSTVE 236

Query: 489  VEGEIFHGAAARSKKQAESNAAKVAYTAFMERKRSRIANXXXXXSNYEATE--VIPGSVS 316
            VEG  FHG A+RSKKQA+ +AAK+AY A  E      A+        +A +  + P  V 
Sbjct: 237  VEGLKFHGKASRSKKQADIDAAKIAYIALKECGLDMYADFSSYNKENQAVQSTLEPEVVK 296

Query: 315  SSPINLHGDFKSDCSAVSTQCANHSGQSFQEKCP----------NLKESS---------- 196
               I    D + D   + T     +     E  P          N++ SS          
Sbjct: 297  HKQILNSEDKRLDQEKLPTDDVKVNDDMHTESLPIPPNKKMKMSNMRTSSLPKSSPLSHT 356

Query: 195  ----------------KTECYLLCNRFKVYTSIPDIDFPKGTCLLPIAEDKWIVVSLECP 64
                             T  YLLC+RF VYT+ PDI FP+   +LP  EDKW+   LE P
Sbjct: 357  ELASSTISDSNMTMTCNTSNYLLCDRFNVYTNFPDILFPEDITVLPFDEDKWVAACLEFP 416


>ref|XP_002319709.1| predicted protein [Populus trichocarpa] gi|222858085|gb|EEE95632.1|
            predicted protein [Populus trichocarpa]
          Length = 240

 Score =  211 bits (536), Expect = 4e-52
 Identities = 116/259 (44%), Positives = 155/259 (59%), Gaps = 5/259 (1%)
 Frame = -3

Query: 1155 MFKSKLQEICHQQKWGLPKYNFIKEGPDHNPSFKASVTVNDVTFDTTIYSKSSKEAQNSA 976
            M+KSKLQE+C Q+ W LP Y   ++G  HNP F A+VTVN+++F +   S +SK+AQN A
Sbjct: 1    MYKSKLQELCQQRAWELPTYESSRQGQAHNPRFLATVTVNNISFHSPSPSNTSKKAQNDA 60

Query: 975  AELAVLHFTASEPRAEPKTGDPVIEIHQAEEMHSDSSVFKNDELNSYKMMLQTYTQRKKV 796
            A+LA  HF+ S P   P    PV ++ +                  +K  LQTY Q++  
Sbjct: 61   AKLAYEHFSISRP--SPSPSPPVSDMQRL-----------------FKSQLQTYAQKRNF 101

Query: 795  GMPVYTSVKTGLPHALCFKATVIVAGHPFESPRSYKTLKEAEHAAAQVALLSFTLDDFQQ 616
             +PVY+  + G PH+  FK  V V G  FES   + TL +AEHAAA+ AL+S   +  ++
Sbjct: 102  ALPVYSCERVGPPHSSRFKCKVTVNGQTFESLEYFSTLNKAEHAAAKAALMSLLPNGVEE 161

Query: 615  IGSPL-----YKNMLQELAQNEGFTLPKYKTEHCFELHYPSFISTVEVEGEIFHGAAARS 451
            +         YKN+LQELAQ EG  LP Y T    E H P+FISTVE+EGEIF G  A++
Sbjct: 162  VSFLFMDESGYKNLLQELAQREGCGLPTYSTNKSGEAHVPTFISTVEIEGEIFTGQGAKT 221

Query: 450  KKQAESNAAKVAYTAFMER 394
            KKQAE +AAK AYTA  +R
Sbjct: 222  KKQAEMSAAKTAYTALKQR 240



 Score = 80.1 bits (196), Expect = 1e-12
 Identities = 51/171 (29%), Positives = 89/171 (52%)
 Frame = -3

Query: 1161 KKMFKSKLQEICHQQKWGLPKYNFIKEGPDHNPSFKASVTVNDVTFDTTIYSKSSKEAQN 982
            +++FKS+LQ    ++ + LP Y+  + GP H+  FK  VTVN  TF++  Y  +  +A++
Sbjct: 85   QRLFKSQLQTYAQKRNFALPVYSCERVGPPHSSRFKCKVTVNGQTFESLEYFSTLNKAEH 144

Query: 981  SAAELAVLHFTASEPRAEPKTGDPVIEIHQAEEMHSDSSVFKNDELNSYKMMLQTYTQRK 802
            +AA+ A++    +              + +   +  D S +KN        +LQ   QR+
Sbjct: 145  AAAKAALMSLLPN-------------GVEEVSFLFMDESGYKN--------LLQELAQRE 183

Query: 801  KVGMPVYTSVKTGLPHALCFKATVIVAGHPFESPRSYKTLKEAEHAAAQVA 649
              G+P Y++ K+G  H   F +TV + G  F + +  KT K+AE +AA+ A
Sbjct: 184  GCGLPTYSTNKSGEAHVPTFISTVEIEGEIF-TGQGAKTKKQAEMSAAKTA 233


>ref|XP_002325855.1| predicted protein [Populus trichocarpa] gi|222862730|gb|EEF00237.1|
            predicted protein [Populus trichocarpa]
          Length = 281

 Score =  190 bits (482), Expect = 7e-46
 Identities = 116/280 (41%), Positives = 162/280 (57%), Gaps = 30/280 (10%)
 Frame = -3

Query: 1155 MFKSKLQEICHQQKWGLPKYNFIKEGPDHNPSFKASVTVNDVTFDTTIYSKSSKEAQNSA 976
            M+KSKLQ++  Q+ W +PKY   KEG +H+P F A+VTV+   F T   S SSK+AQN+A
Sbjct: 1    MYKSKLQQLSQQRGWEIPKYEVTKEGQEHSPHFYATVTVDATLFSTPFPSSSSKKAQNAA 60

Query: 975  AELAVLHFTASEPRAEPKT----------------------GDPVIEIHQAEEM---HSD 871
            A+LA  +F+   PRA   +                      G+  +++  A      +  
Sbjct: 61   AKLAHNYFS-DHPRASSSSPLNVSADCSGGSAGENTRPSPGGNLELDLQDANRTPLSNEA 119

Query: 870  SSVFKNDE-----LNSYKMMLQTYTQRKKVGMPVYTSVKTGLPHALCFKATVIVAGHPFE 706
             +V K DE     L+ +K  LQTY Q++    PVY+  + G PHA+ FK  V V G  +E
Sbjct: 120  GAVAKTDESFGGILHLFKNQLQTYAQKRNFTRPVYSCERVGPPHAIRFKCKVTVNGQTYE 179

Query: 705  SPRSYKTLKEAEHAAAQVALLSFTLDDFQQIGSPLYKNMLQELAQNEGFTLPKYKTEHCF 526
            S   + TL +AE+AAA+ AL+S   +  ++  S  YKN+LQ++AQ EG  LP Y TE   
Sbjct: 180  SREYFPTLSKAENAAAKAALMSLLPNGVEEDESG-YKNLLQDMAQREGCGLPTYFTEKSG 238

Query: 525  ELHYPSFISTVEVEGEIFHGAAARSKKQAESNAAKVAYTA 406
            E H P+FISTVE++G  F G  AR+KKQAE +AAK+AYTA
Sbjct: 239  EAHAPTFISTVEIDGVNFTGKEARNKKQAEMSAAKIAYTA 278


>ref|XP_003592251.1| Double-stranded RNA binding protein [Medicago truncatula]
            gi|357462133|ref|XP_003601348.1| Double-stranded RNA
            binding protein [Medicago truncatula]
            gi|355481299|gb|AES62502.1| Double-stranded RNA binding
            protein [Medicago truncatula] gi|355490396|gb|AES71599.1|
            Double-stranded RNA binding protein [Medicago truncatula]
          Length = 443

 Score =  168 bits (426), Expect = 2e-39
 Identities = 111/283 (39%), Positives = 152/283 (53%), Gaps = 29/283 (10%)
 Frame = -3

Query: 1158 KMFKSKLQEICHQQKWGLPKYNFIK-EGPDHNPSFKASVTVNDVTFDTTIYSKSSKEAQN 982
            KM+KS+LQE CH++KW LP+Y+ I  +GP HNPSFK SV VN +TF ++    SSKEA N
Sbjct: 32   KMYKSRLQEFCHRRKWSLPEYSSIYVDGPPHNPSFKGSVFVNGLTFTSSDIFHSSKEAHN 91

Query: 981  SAAELAVLHFT---------ASEPRAEPKTGDPVIEIHQAEEMHSDSSVFKNDELNSYKM 829
             AA  A+L+F+          +E  ++ K G    +         D  +   D LN  K+
Sbjct: 92   QAAMKALLNFSYPSSSSSMPTNEYGSKEKVGAAKPQKCPVPSQFPDI-LSDTDRLN--KL 148

Query: 828  MLQTYTQRKKVGMPVYTSVKTGLPHALCFKATVIVAGHPFESPRSYKTLKEAEHAAAQV- 652
              Q Y  +  +  PV+T    G P  + + ATV+V G  F+SP S+ T KEAE AA Q+ 
Sbjct: 149  QHQNYASKNNLDSPVFTIEAEGPPRDIRYNATVVVDGKSFKSPTSFDTRKEAEQAALQIV 208

Query: 651  -------ALLSFTLDDFQQIGSPLY-----------KNMLQELAQNEGFTLPKYKTEHCF 526
                   AL       F++  S L            K++LQEL Q    ++P YK+    
Sbjct: 209  DMFQARSALAFKPCQTFEKPRSALNNRHMTNETCASKSLLQELTQRRYCSIPTYKSTRTG 268

Query: 525  ELHYPSFISTVEVEGEIFHGAAARSKKQAESNAAKVAYTAFME 397
              H P+F STVEVEG  FHG A+ SKK+AE +AAK+AY A  +
Sbjct: 269  PPHMPTFFSTVEVEGVEFHGKASSSKKEAEYDAAKIAYKALKD 311