BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00018512
         (1928 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002268993.1| PREDICTED: NEDD8 ultimate buster 1 [Vitis vi...   622   e-176
ref|XP_002532310.1| NEDD8 ultimate buster, putative [Ricinus com...   580   e-163
ref|XP_002324589.1| predicted protein [Populus trichocarpa] gi|2...   565   e-158
ref|XP_002331011.1| predicted protein [Populus trichocarpa] gi|2...   558   e-156
ref|XP_004139279.1| PREDICTED: NEDD8 ultimate buster 1-like [Cuc...   557   e-156

>ref|XP_002268993.1| PREDICTED: NEDD8 ultimate buster 1 [Vitis vinifera]
            gi|296084548|emb|CBI25569.3| unnamed protein product
            [Vitis vinifera]
          Length = 552

 Score =  622 bits (1605), Expect = e-176
 Identities = 331/565 (58%), Positives = 414/565 (73%), Gaps = 3/565 (0%)
 Frame = -1

Query: 1907 KLKIAGAWSGVLEVELETWSVIMLKDEVAKRLGCGPESINLISAGKVLKDGDDQNLTQLG 1728
            KLKIAGAWSGVLE+EL+ W+V ML++EVAKR  CGPESINLI +GK+LKD ++  L +LG
Sbjct: 3    KLKIAGAWSGVLELELQVWTVSMLREEVAKRASCGPESINLIWSGKLLKD-ENLKLIELG 61

Query: 1727 LKNNSKIMATRVSADQGKALMLEDQKAMAEEERSRRLSRIKAAATSLAKRHADGSLPVED 1548
            +KNN K++ATRV +DQGK+L    ++ MAEEERS RL+R+KAAA +L+KRHA GSLPVED
Sbjct: 62   IKNNGKVLATRVCSDQGKSLK---EEFMAEEERSSRLARVKAAAIALSKRHAAGSLPVED 118

Query: 1547 FNLELENQSGEKVQLGTENDQRAIMTGLMLQANAKQLIKRQKYKDALEVLAMGEEAFSIC 1368
            FN+ELE+QSG+KV LG+E DQRA+M GLML ANAKQ+IK++ YKDALEVL+MGEEAFS+C
Sbjct: 119  FNIELEDQSGQKVHLGSETDQRAVMMGLMLHANAKQMIKKENYKDALEVLSMGEEAFSLC 178

Query: 1367 DQKLIEMVDNVSILQIDMVWCYFMLKDISWLSVAGVRLAKAREGIERAHGKESIRVRILQ 1188
            + K+IE+VDNV ILQIDMVWCYFML+DI WLS AG+RLAKAREGIER+HGK++ RVR+LQ
Sbjct: 179  NPKVIELVDNVPILQIDMVWCYFMLRDIRWLSEAGIRLAKAREGIERSHGKDASRVRVLQ 238

Query: 1187 GGHCPELPLYLRMDLLEGVVAYHSGQLQKSKASLTSAQTKYMRLQVPDELLSLLMSMGYK 1008
             G  PEL L+LR++LLEGVV++HSGQ +KSK  LTSAQ KY +LQVPDE LSLLMSMGYK
Sbjct: 239  NGRYPELALHLRLELLEGVVSFHSGQFEKSKKVLTSAQAKYFQLQVPDEALSLLMSMGYK 298

Query: 1007 QNDAKRALRMSGQDVGSAVXXXXXXXXXXXXXXXXXXXXXXEIMEQKRYGKTPLGKAVDL 828
            + ++KRALRMS Q +  A+                      EIMEQKRYG TPL KAVDL
Sbjct: 299  ERNSKRALRMSNQQLDLAIDFLVEEKAKKEKKREEDIQRRNEIMEQKRYGMTPLRKAVDL 358

Query: 827  QRLNELESIGFEKALAAEALRRNENDTQKALDDLTNPETNASLQLNVESNRRKRQRQEAD 648
            Q L+ L SIGFEK LAAEALRRNENDTQKALDDLTNPETN+++QL++ES +RKRQRQ  +
Sbjct: 359  QSLHMLVSIGFEKELAAEALRRNENDTQKALDDLTNPETNSAIQLDIESRKRKRQRQANE 418

Query: 647  AVIQKLVSMGFPRANATAAVNEYGTEVEALNHLLAVQSNPXXXXXXXXXXXXXXXXSHPM 468
            A I++L+SMGF R+    AV  +GT+ +ALN LL                       +P 
Sbjct: 419  AAIEELISMGFERSEVVTAVQTFGTKEQALNALLGTAKT------------------NPT 460

Query: 467  LRADGENVFGSQLP---VVNQTSGDGSNGGPSTSIQXXXXXXXXXXXXXXELHKGDALSD 297
               D   +    +P    +   S  G  GG S++ +              +L  GDA SD
Sbjct: 461  APDDSNVIPSGSMPDEASLGSWSEVGDAGGSSSAKKGEERDLEMEDELAEDLRNGDAFSD 520

Query: 296  YDIDVTKEGEAINEYLAMITNAEEN 222
            YD++VTKEGEAINEYL ++ +A+++
Sbjct: 521  YDMEVTKEGEAINEYLTLLASADQS 545


>ref|XP_002532310.1| NEDD8 ultimate buster, putative [Ricinus communis]
            gi|223527979|gb|EEF30062.1| NEDD8 ultimate buster,
            putative [Ricinus communis]
          Length = 559

 Score =  580 bits (1495), Expect = e-163
 Identities = 316/565 (55%), Positives = 399/565 (70%), Gaps = 2/565 (0%)
 Frame = -1

Query: 1910 AKLKIAGAWSGVLEVELETWSVIMLKDEVAKRLGCGPESINLISAGKVLKDGD-DQNLTQ 1734
            AKLKIAG W GV+EVELE WSV ML+ EV+K+   G +SINLI AGKVLKDGD  + L Q
Sbjct: 6    AKLKIAGIWVGVIEVELENWSVSMLRQEVSKQSNMGTDSINLICAGKVLKDGDGSEKLRQ 65

Query: 1733 LGLKNNSKIMATRVSADQGKALMLEDQKAMAEEERSRRLSRIKAAATSLAKRHADGSLPV 1554
            LG+KNNSKI+A+RVS ++GK+L    Q+ MA+EER+RRL+R+KAA T+L++RH DGSLP+
Sbjct: 66   LGVKNNSKILASRVSVEEGKSLK---QELMADEERNRRLARVKAAVTALSRRHGDGSLPL 122

Query: 1553 EDFNLELENQSGEKVQLGTENDQRAIMTGLMLQANAKQLIKRQKYKDALEVLAMGEEAFS 1374
            +DFN+ELE+QSG+KV   +E D++AIM GLML AN K LI+RQ +KDALEVL MGEEAFS
Sbjct: 123  DDFNIELEDQSGQKVHF-SETDRQAIMMGLMLHANGKGLIRRQMFKDALEVLTMGEEAFS 181

Query: 1373 ICDQKLIEMVDNVSILQIDMVWCYFMLKDISWLSVAGVRLAKAREGIERAHGKESIRVRI 1194
            +C+ K IE+VDN+SILQIDMVWCYFML+DI+WLSVAGVRL KAREG+ERAHGK+S R R+
Sbjct: 182  LCNPKSIELVDNISILQIDMVWCYFMLRDIAWLSVAGVRLEKAREGLERAHGKDSSRFRL 241

Query: 1193 LQGGHCPELPLYLRMDLLEGVVAYHSGQLQKSKASLTSAQTKYMRLQVPDELLSLLMSMG 1014
            LQ G   EL LYLR++LLEGVVAYHSGQ  K +  LTSAQ ++ +L+VPDE LS++M MG
Sbjct: 242  LQAGRSSELSLYLRLELLEGVVAYHSGQFDKCRKYLTSAQERFFQLRVPDEALSIVMGMG 301

Query: 1013 YKQNDAKRALRMSGQDVGSAVXXXXXXXXXXXXXXXXXXXXXXEIMEQKRYGKTPLGKAV 834
            +K+NDAKRALRMS QD+ SA+                      EI EQ+RYG+T L KAV
Sbjct: 302  FKENDAKRALRMSNQDIESAINFLVEEKERKAKKMEDDIRRRAEIREQRRYGETALKKAV 361

Query: 833  DLQRLNELESIGFEKALAAEALRRNENDTQKALDDLTNPETNASLQLNVESNRRKRQRQE 654
            DLQRL EL S+GFEK LAAEALRRNEND++KALDDLTNP+TN ++Q ++ES +RKR +++
Sbjct: 362  DLQRLKELVSLGFEKELAAEALRRNENDSEKALDDLTNPQTNTAIQHDIESRKRKRNQRK 421

Query: 653  ADAVIQKLVSMGFPRANATAAVNEYGTEVEALNHLL-AVQSNPXXXXXXXXXXXXXXXXS 477
                I++LVSMGF R+    AV   G+  +A+  LL    +NP                +
Sbjct: 422  VGTKIEQLVSMGFERSRVVEAVRAGGSVEQAMQQLLTGSMTNPTASATNSDSISRPTSDA 481

Query: 476  HPMLRADGENVFGSQLPVVNQTSGDGSNGGPSTSIQXXXXXXXXXXXXXXELHKGDALSD 297
               L  D               S D    GPS S +              E+ KGDALSD
Sbjct: 482  VDSLNQD-------------NLSDDNDTEGPSAS-EVEQRDLEMEDTIAEEIAKGDALSD 527

Query: 296  YDIDVTKEGEAINEYLAMITNAEEN 222
            YDI+VTKEGEAINEY+A++ + + +
Sbjct: 528  YDIEVTKEGEAINEYMALLNSVDSS 552


>ref|XP_002324589.1| predicted protein [Populus trichocarpa] gi|222866023|gb|EEF03154.1|
            predicted protein [Populus trichocarpa]
          Length = 578

 Score =  565 bits (1457), Expect = e-158
 Identities = 313/571 (54%), Positives = 397/571 (69%), Gaps = 10/571 (1%)
 Frame = -1

Query: 1910 AKLKIAGAWSGVLEVELETWSVIMLKDEVAKRLGCGPESINLISAGKVLKD---GDDQNL 1740
            AKLK+AG W+G+LEVE E W+V ML++E+AKR   G ESINLI AGKVLKD    +  +L
Sbjct: 10   AKLKVAGTWAGLLEVETENWTVPMLREEIAKRSNMGTESINLIFAGKVLKDCTSEEKNSL 69

Query: 1739 TQLGLKNNSKIMATRVSADQGKALMLEDQKAMAEEERSRRLSRIKAAATSLAKRHADGSL 1560
            +QLG+KNNSKI+A RVS ++GK L  E    +A++ER+ RL+RI+AA T+L+KRHADG+L
Sbjct: 70   SQLGIKNNSKILACRVSVEEGKTLKNE---LLADDERNHRLARIRAAVTALSKRHADGAL 126

Query: 1559 PVEDFNLELENQSGEKVQLGTENDQRAIMTGLMLQANAKQLIKRQKYKDALEVLAMGEEA 1380
            P+EDF++ELE+QSG+KV   +E D+ AIMTGLML  + K+ I++Q + DALEVL MGEEA
Sbjct: 127  PIEDFDIELEDQSGKKVHF-SETDRLAIMTGLMLHTSGKRFIRKQMFTDALEVLTMGEEA 185

Query: 1379 FSICDQKLIEMVDNVSILQIDMVWCYFMLKDISWLSVAGVRLAKAREGIERAHGKESIRV 1200
            FS+C+ K IE+VDN+ ILQIDMVWCYFML+DI+W++VAG+RL KAREG+ERAHGK+S R 
Sbjct: 186  FSLCNPKSIELVDNIPILQIDMVWCYFMLRDIAWIAVAGLRLKKAREGLERAHGKDSSRF 245

Query: 1199 RILQGGHCPELPLYLRMDLLEGVVAYHSGQLQKSKASLTSAQTKYMRLQVPDELLSLLMS 1020
            R+LQ G   EL LYLR++LLEGVVAYHSGQ  KS+  L SAQ K+ +LQVPDE LSL+MS
Sbjct: 246  RLLQAGRTSELALYLRLELLEGVVAYHSGQFNKSRKFLASAQEKFFQLQVPDEALSLVMS 305

Query: 1019 MGYKQNDAKRALRMSGQDVGSAVXXXXXXXXXXXXXXXXXXXXXXEIMEQKRYGKTPLGK 840
            MG+ + DAKRALRMS QD+ SAV                      EIMEQKRYG TPL K
Sbjct: 306  MGFGEWDAKRALRMSNQDIQSAVNFLVVEREKREQKREDDIRRRNEIMEQKRYGVTPLKK 365

Query: 839  AVDLQRLNELESIGFEKALAAEALRRNENDTQKALDDLTNPETNASLQLNVESNRRKRQR 660
            AVDLQRL E+ SIGFEK LAAEALR+NENDTQKALDDLTNPE N +LQ N+E  +R+RQ+
Sbjct: 366  AVDLQRLTEVVSIGFEKELAAEALRKNENDTQKALDDLTNPEANTALQRNIELRKRRRQQ 425

Query: 659  QEADAVIQKLVSMGFPRANATAAVNEYGTEVEALNHLLAVQSNPXXXXXXXXXXXXXXXX 480
            +  DA I++LVSMGF R+    AV   G+  +A++ LL                      
Sbjct: 426  RATDATIERLVSMGFERSRVIGAVQAGGSLEQAMHQLLTYSRADTTVAAENSANAHVSAV 485

Query: 479  SHPMLRADG-------ENVFGSQLPVVNQTSGDGSNGGPSTSIQXXXXXXXXXXXXXXEL 321
            ++     D        +N+    L +      D SN GPS + +              EL
Sbjct: 486  NNNASAPDSTPTDLALDNLDPDALDI------DCSNEGPSAA-EIEQRDVEMEGEIADEL 538

Query: 320  HKGDALSDYDIDVTKEGEAINEYLAMITNAE 228
             +GDALSDYDI+V KEGEAINEYLA++ +A+
Sbjct: 539  ARGDALSDYDIEVAKEGEAINEYLALLASAD 569


>ref|XP_002331011.1| predicted protein [Populus trichocarpa] gi|222872941|gb|EEF10072.1|
            predicted protein [Populus trichocarpa]
          Length = 566

 Score =  558 bits (1437), Expect = e-156
 Identities = 313/571 (54%), Positives = 394/571 (69%), Gaps = 14/571 (2%)
 Frame = -1

Query: 1910 AKLKIAGAWSGVLEVELETWSVIMLKDEVAKRLGCGPESINLISAGKVLKD---GDDQNL 1740
            AKLK+AG W+GVLEVE E W+V+ML++E+AKR   G ESINLI AGKVLKD    +  +L
Sbjct: 2    AKLKVAGTWAGVLEVEPENWTVLMLREEIAKRSNMGTESINLIFAGKVLKDCTSEEKNSL 61

Query: 1739 TQLGLKNNSKIMATRVSADQGKALMLEDQKAMAEEERSRRLSRIKAAATSLAKRHADGSL 1560
            +QLG+KNNSKI+A RVS ++GK L  E    +A++ER+RRL+RIKAA T+L+KRHADG+L
Sbjct: 62   SQLGIKNNSKILACRVSVEEGKTLKNE---LLADDERNRRLARIKAAVTALSKRHADGAL 118

Query: 1559 PVEDFNLELENQSGEKVQLGTENDQRAIMTGLMLQANAKQLIKRQKYKDALEVLAMGEEA 1380
            P+EDF++ELE+QSG+KV   +E D++AIMTGLML  + K+ I++Q + DALEVL MGEEA
Sbjct: 119  PIEDFDIELEDQSGKKVHF-SETDRQAIMTGLMLHTSGKRFIRKQMFSDALEVLTMGEEA 177

Query: 1379 FSICDQKLIEMVDNVSILQIDMVWCYFMLKDISWLSVAGVRLAKAREGIERAHGKESIRV 1200
            FS+C+ K IE+VDN+ ILQIDMVWCYFML+DI+WLSVAG+RL KAREG+ERAHGK+S R 
Sbjct: 178  FSLCNPKSIELVDNIPILQIDMVWCYFMLQDIAWLSVAGLRLEKAREGLERAHGKDSSRF 237

Query: 1199 RILQGGHCPELPLYLRMDLLEGVVAYHSGQLQKSKASLTSAQTKYMRLQVPDELLSLLMS 1020
            R+LQ G   EL LYLR++LLEGVVAYHSGQ  KS+  LTSAQ K+ +LQVPDE LS++MS
Sbjct: 238  RLLQAGCSSELALYLRLELLEGVVAYHSGQFDKSRKFLTSAQAKFFQLQVPDEALSVVMS 297

Query: 1019 MGYKQNDAKRALRMSGQDVGSAVXXXXXXXXXXXXXXXXXXXXXXEIMEQKRYGKTPLGK 840
            +G+ + DAKRALRMS QDV SAV                      EI +QKRYG TPL K
Sbjct: 298  IGFGEQDAKRALRMSNQDVQSAVNFLVLEREKREQKREDDIRRRNEIKQQKRYGVTPLKK 357

Query: 839  AVDLQRLNELESIGFEKALAAEALRRNENDTQKALDDLTNPETNASLQLNVESNRRKRQR 660
            AVDLQ+L EL SIGFEK LAAEALR+NEN +Q+ALDDLTNPE N  LQ  +ES +RKRQ+
Sbjct: 358  AVDLQKLTELVSIGFEKELAAEALRKNENGSQEALDDLTNPEANTELQRAIESRKRKRQQ 417

Query: 659  QEADAVIQKLVSMGFPRANATAAVNEYGTEVEALNHLLA---VQSNPXXXXXXXXXXXXX 489
            +  DA +++LVSMGF R+    AV   G+  E ++ LL      +               
Sbjct: 418  RATDATVEQLVSMGFERSRVIGAVQAGGSLEEVMHQLLTHPQADTTAAADDSSNAHDSSG 477

Query: 488  XXXSHPMLRADGENVFGSQLP-------VVNQTSG-DGSNGGPSTSIQXXXXXXXXXXXX 333
               +   L A+         P       +   T G D SN GPS                
Sbjct: 478  NNNASTDLNANDNACAPDSTPTNPAVENLSPDTLGIDNSNEGPSAE----QRDLEMEDEI 533

Query: 332  XXELHKGDALSDYDIDVTKEGEAINEYLAMI 240
              EL +GDALSDY+IDVT+EG+AINEYLA++
Sbjct: 534  ADELTRGDALSDYNIDVTQEGDAINEYLALL 564


>ref|XP_004139279.1| PREDICTED: NEDD8 ultimate buster 1-like [Cucumis sativus]
          Length = 556

 Score =  557 bits (1436), Expect = e-156
 Identities = 319/569 (56%), Positives = 394/569 (69%), Gaps = 5/569 (0%)
 Frame = -1

Query: 1910 AKLKIAGAWSGVLEVELETWSVIMLKDEVAKRLGCGPESINLISAGKVLKDGD-DQNLTQ 1734
            AKLKI+G W+GVL+VEL+ W+V+ML++E+A+R  CG ESINLIS G++LKDGD  + L  
Sbjct: 2    AKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLVH 61

Query: 1733 LGLKNNSKIMATRVSADQGKALMLEDQKAMAEEERSRRLSRIKAAATSLAKRHADGSLPV 1554
            LGLKNNS+I+A RV+A++GKAL  E    MAEEERS RL+R+KAAAT+LA+RHA+G LPV
Sbjct: 62   LGLKNNSRILARRVAAEEGKALKNE---LMAEEERSSRLARVKAAATALAERHANGFLPV 118

Query: 1553 EDFNLELENQSGEKVQLGTENDQRAIMTGLMLQANAKQLIKRQKYKDALEVLAMGEEAFS 1374
            EDFN+ELE+QSG+KV  G+E DQ+AIM GLML ANAKQLI++  YKDALEVL MGEEAF+
Sbjct: 119  EDFNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFT 178

Query: 1373 ICDQKLIEMVDNVSILQIDMVWCYFMLKDISWLSVAGVRLAKAREGIERAHGKESIRVRI 1194
            +C+ KLIE VDNVSILQIDMVWCYFMLKDI WLS AG+RLAKAREGIERAHGK+S RVRI
Sbjct: 179  LCNPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRI 238

Query: 1193 LQGGHCPELPLYLRMDLLEGVVAYHSGQLQKSKASLTSAQTKYMRLQVPDELLSLLMSMG 1014
            LQ G  PEL L+LR++LLEGV AYHS +  KS+ ++ SA+ KY +LQV D+ LS +M MG
Sbjct: 239  LQAGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMG 298

Query: 1013 YKQNDAKRALRMSGQDVGSAVXXXXXXXXXXXXXXXXXXXXXXEIMEQKRYGKTPLGKAV 834
            +K+ +AKRALRM  QDV  AV                      EIMEQK YG TP  KAV
Sbjct: 299  FKEKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAV 358

Query: 833  DLQRLNELESIGFEKALAAEALRRNENDTQKALDDLTNPETNASLQLNVESNRRKRQRQE 654
            +LQ L +L SIG+EK LAAEALRRNEND QKALDDL +PE+N+ +Q  VES +RKRQR+ 
Sbjct: 359  NLQILEKLASIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKR 418

Query: 653  ADAVIQKLVSMGFPRANATAAVNEYGTEVEALNHLLAVQSNPXXXXXXXXXXXXXXXXSH 474
            AD  + KLV MGF +  A AA    G    +L   + + S+P                 +
Sbjct: 419  ADDAVDKLVCMGFDKTRAEAAFEAGG----SLEQAVIILSDP----GTNSTVDGQPDSVN 470

Query: 473  PMLRADGENVFGSQLPVVNQTSGD--GSNGGPSTSIQXXXXXXXXXXXXXXELHKGDALS 300
              L ++G +   S +   NQ   D     GGP  SI               EL+   A+S
Sbjct: 471  AALTSEGASSSLSNI-AENQEMLDTLDDEGGP--SIGEEDRDLEMEDELARELNGATAVS 527

Query: 299  DYDIDVTKEGEAINEYLAMITNA--EENA 219
            DYD+DVTKEGEAI EYL ++ +    ENA
Sbjct: 528  DYDMDVTKEGEAITEYLGLLDSMMNSENA 556