BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00018279
         (2016 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002284299.1| PREDICTED: tRNA (guanine-N(1)-)-methyltransf...   615   e-173
ref|XP_002525790.1| conserved hypothetical protein [Ricinus comm...   603   e-170
ref|XP_004146841.1| PREDICTED: tRNA (guanine(37)-N1)-methyltrans...   585   e-164
ref|XP_003539574.1| PREDICTED: tRNA (guanine-N(1)-)-methyltransf...   566   e-159
ref|XP_003543273.1| PREDICTED: tRNA (guanine-N(1)-)-methyltransf...   563   e-158

>ref|XP_002284299.1| PREDICTED: tRNA (guanine-N(1)-)-methyltransferase-like [Vitis
            vinifera] gi|363805566|sp|F6H2F8.1|TRM51_VITVI RecName:
            Full=tRNA (guanine(37)-N1)-methyltransferase 1; AltName:
            Full=M1G-methyltransferase 1; AltName: Full=tRNA [GM37]
            methyltransferase 1; AltName: Full=tRNA methyltransferase
            5 homolog 1
          Length = 608

 Score =  615 bits (1587), Expect = e-173
 Identities = 305/441 (69%), Positives = 358/441 (81%)
 Frame = -3

Query: 2002 SLNRRIYGKSERDGERLSPVLYRDKLVNTFDARGFVKFRNLAKLSRPKRRKKQDIVGEKQ 1823
            SLNRRIYGK+E DGE LSPVLYRD L  TFD++GF  FRNLAKLSRPK++K++      +
Sbjct: 163  SLNRRIYGKAEGDGEILSPVLYRDTLAKTFDSQGFANFRNLAKLSRPKKKKRRKEEERSE 222

Query: 1822 VREKKGGEVYAXXXXXXXXXXXXXXXXXXXXDFSGRKWRGSTRLLLLDERYGDKELDEMP 1643
             +++ G   +A                    +F  ++WRGSTRLLLLDERY DK ++E+P
Sbjct: 223  GKKRTGKNEFAMVEVVEDGEEGEDLRGLLGEEFKRKRWRGSTRLLLLDERYADKGVEELP 282

Query: 1642 EAIKAVFNKGGSESMTSAIELVRCNLTLFYTYWQMNEILEALLPAGLIIPSAFETVGHIA 1463
            EAIKAV  +   +SMTS  ELV+C LTLFY YWQMNEILEALLP G+I+PSAFE VGHIA
Sbjct: 283  EAIKAVLKEDTGQSMTSTFELVKCKLTLFYNYWQMNEILEALLPEGMIVPSAFEMVGHIA 342

Query: 1462 HLNLRDEHHSHKKLIAKVVLDKNKPKIQTVVNKIDAIHNDYRTMQLEVLAGNHSLVTTVV 1283
            HLNLRDEH  +KKLIAKVVLDKNKPKIQTVVNK DAIHNDYRTMQLEVLAGN SLVTTV+
Sbjct: 343  HLNLRDEHLPYKKLIAKVVLDKNKPKIQTVVNKTDAIHNDYRTMQLEVLAGNRSLVTTVI 402

Query: 1282 ENGLRFHVDLATVYWNSKLATERQRLVNCFTPSDIVCDVFAGVGPIAIAAAKRVKHVYAN 1103
            ENG+RF VDLATVYWNS+LATERQRL+NCFT +D+VCDVF+GVGPIAI+AAK+VK VYAN
Sbjct: 403  ENGMRFQVDLATVYWNSRLATERQRLLNCFTRNDVVCDVFSGVGPIAISAAKKVKRVYAN 462

Query: 1102 DLNPDAVDFLQRNCVLNKLERKIEVFNMDGRRFIDSVFARLKARCITQVVMNLPNDAAEY 923
            DLNP A+++L+ N VLNKLERKI+VFNMDGRRFI+++F   KA  ITQVVMNLPNDAAE+
Sbjct: 463  DLNPYAIEYLESNSVLNKLERKIKVFNMDGRRFINAMFTSDKAESITQVVMNLPNDAAEF 522

Query: 922  LDVFRGLFGDYNTAKTLTLPKVHVYGFSKAQDPEFEFQERIRIALSEVAFEIQMHRVRLV 743
            LD FRG+F   +  K L LP +HVYGFSKAQDPEF+F +RIRIALSEVA +++MHRVRLV
Sbjct: 523  LDAFRGIFRKKSRDKQLKLPMIHVYGFSKAQDPEFDFHQRIRIALSEVAVDVEMHRVRLV 582

Query: 742  APGKWMMCASFVLPERVAFTK 680
            APGKWM+ ASF+LP+ V F K
Sbjct: 583  APGKWMLRASFILPKSVVFAK 603


>ref|XP_002525790.1| conserved hypothetical protein [Ricinus communis]
            gi|223534940|gb|EEF36626.1| conserved hypothetical
            protein [Ricinus communis]
          Length = 641

 Score =  603 bits (1554), Expect = e-170
 Identities = 305/444 (68%), Positives = 358/444 (80%), Gaps = 3/444 (0%)
 Frame = -3

Query: 2008 LVSLNRRIYGKSERDGERLSPVLYRDKLVNTFDARGFVKFRNLAKLSRPKRRKKQD--IV 1835
            L +L+RRIYG++E DGE LSPVLYR++L   F++RGF KFRNLAK+SRP +RK+++  + 
Sbjct: 192  LDALDRRIYGRAEGDGEELSPVLYRERLAKEFNSRGFFKFRNLAKISRPPKRKRKEGEVE 251

Query: 1834 GEKQVREKKGGEVYAXXXXXXXXXXXXXXXXXXXXDFSG-RKWRGSTRLLLLDERYGDKE 1658
            G +++R+K G + +A                    DF G +KWRGSTRLLLLDERY DK 
Sbjct: 252  GGEKMRKKNGKDQFAVVEVMEADDEEGDWKGLLGDDFKGSKKWRGSTRLLLLDERYADKR 311

Query: 1657 LDEMPEAIKAVFNKGGSESMTSAIELVRCNLTLFYTYWQMNEILEALLPAGLIIPSAFET 1478
            ++++P AIKA   +   E+ TS  ELVRC LTL Y YWQMNE+LEALLP G IIPSAFET
Sbjct: 312  MEDLPRAIKAALQEAMKENSTSTFELVRCKLTLSYNYWQMNEVLEALLPEGAIIPSAFET 371

Query: 1477 VGHIAHLNLRDEHHSHKKLIAKVVLDKNKPKIQTVVNKIDAIHNDYRTMQLEVLAGNHSL 1298
            VGHIAHLNLR+EH  +KKLIAKVVLDKNKPKIQTVVNKIDAI NDYRTMQLEVLAGNHSL
Sbjct: 372  VGHIAHLNLRNEHMPYKKLIAKVVLDKNKPKIQTVVNKIDAIQNDYRTMQLEVLAGNHSL 431

Query: 1297 VTTVVENGLRFHVDLATVYWNSKLATERQRLVNCFTPSDIVCDVFAGVGPIAIAAAKRVK 1118
            VT VVENGLRFHVDLA VYWNS+LATERQRL++ FT +D+VCDVF+GVGPIA++AA  VK
Sbjct: 432  VTMVVENGLRFHVDLAKVYWNSRLATERQRLLDGFTRNDVVCDVFSGVGPIALSAATIVK 491

Query: 1117 HVYANDLNPDAVDFLQRNCVLNKLERKIEVFNMDGRRFIDSVFARLKARCITQVVMNLPN 938
             VYANDLNP AVD+L+RN VLNKLERK++VFNMDGRRFID++F+  KA+ ITQVVMNLPN
Sbjct: 492  RVYANDLNPCAVDYLERNSVLNKLERKVKVFNMDGRRFIDAMFSSDKAQTITQVVMNLPN 551

Query: 937  DAAEYLDVFRGLFGDYNTAKTLTLPKVHVYGFSKAQDPEFEFQERIRIALSEVAFEIQMH 758
            DAAEYLD FRGLF D    + + LP +HVYGFSKA DPEFEF ERIRIAL+E A  ++M 
Sbjct: 552  DAAEYLDAFRGLFKDNPKKREVALPMIHVYGFSKAHDPEFEFHERIRIALAEAAVHVEMR 611

Query: 757  RVRLVAPGKWMMCASFVLPERVAF 686
            RVRLVAPGKWM+CASF LPE VAF
Sbjct: 612  RVRLVAPGKWMLCASFRLPESVAF 635


>ref|XP_004146841.1| PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 1-like [Cucumis
            sativus]
          Length = 626

 Score =  585 bits (1507), Expect = e-164
 Identities = 301/445 (67%), Positives = 354/445 (79%), Gaps = 1/445 (0%)
 Frame = -3

Query: 2011 SLVSLNRRIYGKSERDGERLSPVLYRDKLVNTFDARGFVKFRNLAKLSRPKRRKKQDIVG 1832
            S+  L+RRIYGK+E DGE LSPVLYR+KLV TFD++G+V FR+LAK+SR KRRK++   G
Sbjct: 176  SIDLLHRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEG 235

Query: 1831 EKQVREKKGGEVYAXXXXXXXXXXXXXXXXXXXXD-FSGRKWRGSTRLLLLDERYGDKEL 1655
              +   + G   +A                    D F G KWRGSTRLLLLDE+Y  K +
Sbjct: 236  RDEKMRRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGV 295

Query: 1654 DEMPEAIKAVFNKGGSESMTSAIELVRCNLTLFYTYWQMNEILEALLPAGLIIPSAFETV 1475
            +E+PEAIKA+      E M   IELVRC LTLFY YWQMNE+L+ALLP  +IIPSAFETV
Sbjct: 296  EELPEAIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETV 355

Query: 1474 GHIAHLNLRDEHHSHKKLIAKVVLDKNKPKIQTVVNKIDAIHNDYRTMQLEVLAGNHSLV 1295
            GHIAHLNLRDEH S+KKLIAKV+LDKNKPKIQTVVNK DAI NDYRTMQLEVLAGNHSLV
Sbjct: 356  GHIAHLNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLV 415

Query: 1294 TTVVENGLRFHVDLATVYWNSKLATERQRLVNCFTPSDIVCDVFAGVGPIAIAAAKRVKH 1115
            TTVVENGLRFH+DLATVYWNS+LATERQRL++ FT +D++CDVFAGVGPI+I+AA+ VK 
Sbjct: 416  TTVVENGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKK 475

Query: 1114 VYANDLNPDAVDFLQRNCVLNKLERKIEVFNMDGRRFIDSVFARLKARCITQVVMNLPND 935
            VYANDLNP AV++L+RN V NKLERKIEVFNM+GRRFI ++FA  KA  ITQVVMNLPND
Sbjct: 476  VYANDLNPYAVEYLERNSVANKLERKIEVFNMEGRRFIRAMFASEKAPKITQVVMNLPND 535

Query: 934  AAEYLDVFRGLFGDYNTAKTLTLPKVHVYGFSKAQDPEFEFQERIRIALSEVAFEIQMHR 755
            AAEYLD F+G+  D  + K  TLP +HVYGFSKA+DPEF+F ERIRIA++EVA ++ + R
Sbjct: 536  AAEYLDAFKGILRD-RSDKEFTLPIIHVYGFSKARDPEFDFHERIRIAITEVAVDVNVRR 594

Query: 754  VRLVAPGKWMMCASFVLPERVAFTK 680
            VRLVAPGKWM+CASF LP  VA +K
Sbjct: 595  VRLVAPGKWMLCASFRLPRSVALSK 619


>ref|XP_003539574.1| PREDICTED: tRNA (guanine-N(1)-)-methyltransferase-like [Glycine max]
          Length = 594

 Score =  566 bits (1459), Expect = e-159
 Identities = 288/443 (65%), Positives = 347/443 (78%), Gaps = 2/443 (0%)
 Frame = -3

Query: 2008 LVSLNRRIYGKSERDGERLSPVLYRDKLVNTFDARGFVKFRNLAKLSRPKRRKKQDIVGE 1829
            LVSL RRI+GK++ DG+ L PVLYRD+L  TF+  GFVKFR+LAK+SRP R K++    E
Sbjct: 144  LVSLQRRIHGKADGDGDVLGPVLYRDRLARTFNTSGFVKFRHLAKISRPNRNKRRRKSRE 203

Query: 1828 KQVREKKGGE--VYAXXXXXXXXXXXXXXXXXXXXDFSGRKWRGSTRLLLLDERYGDKEL 1655
                 K  GE    A                    +F+  KWRGSTRLLLLDERY  + +
Sbjct: 204  ASEEVKPVGENRFAAVEVVEDEEEGSDGLRNLLGEEFARSKWRGSTRLLLLDERYAGRGM 263

Query: 1654 DEMPEAIKAVFNKGGSESMTSAIELVRCNLTLFYTYWQMNEILEALLPAGLIIPSAFETV 1475
            +E+PEAIKAV  +   +S    +ELVRC LTLFY YWQM E+LEALLP G+IIPSAFETV
Sbjct: 264  EELPEAIKAVLKEYAEKSTNLTLELVRCKLTLFYDYWQMTEVLEALLPEGMIIPSAFETV 323

Query: 1474 GHIAHLNLRDEHHSHKKLIAKVVLDKNKPKIQTVVNKIDAIHNDYRTMQLEVLAGNHSLV 1295
            GHIAHLNLRDEH  +K+LIAKVVLDKNKP+IQTVVNKID+I N+YRTMQLEVLAGNHSLV
Sbjct: 324  GHIAHLNLRDEHLPYKRLIAKVVLDKNKPRIQTVVNKIDSIQNEYRTMQLEVLAGNHSLV 383

Query: 1294 TTVVENGLRFHVDLATVYWNSKLATERQRLVNCFTPSDIVCDVFAGVGPIAIAAAKRVKH 1115
            TTVVENGLRF VDLATVYW+S+L TERQRL++ FT +D+VCDVF+GVGP+AI+AA+ VK 
Sbjct: 384  TTVVENGLRFQVDLATVYWSSRLGTERQRLLSGFTRNDVVCDVFSGVGPLAISAARIVKR 443

Query: 1114 VYANDLNPDAVDFLQRNCVLNKLERKIEVFNMDGRRFIDSVFARLKARCITQVVMNLPND 935
            V+ANDLNP AV++L+RNCVLNKLERKI+VFNMDGRRFI +++    ++ ITQVVMNLP++
Sbjct: 444  VFANDLNPYAVEYLERNCVLNKLERKIKVFNMDGRRFIKAMYDSDISQSITQVVMNLPSE 503

Query: 934  AAEYLDVFRGLFGDYNTAKTLTLPKVHVYGFSKAQDPEFEFQERIRIALSEVAFEIQMHR 755
            AAE+LD FRG++ +       TLP +HVYGFSKA+DPEF+F ERIRIAL EVA  + M R
Sbjct: 504  AAEFLDAFRGIYKNRPKDGEYTLPLIHVYGFSKARDPEFDFHERIRIALLEVAVNVDMRR 563

Query: 754  VRLVAPGKWMMCASFVLPERVAF 686
            VRLVAPGKWM+CASF+LP+ VAF
Sbjct: 564  VRLVAPGKWMLCASFILPKSVAF 586


>ref|XP_003543273.1| PREDICTED: tRNA (guanine-N(1)-)-methyltransferase-like [Glycine max]
          Length = 601

 Score =  563 bits (1451), Expect = e-158
 Identities = 288/448 (64%), Positives = 348/448 (77%), Gaps = 7/448 (1%)
 Frame = -3

Query: 2008 LVSLNRRIYGKSERDGERLSPVLYRDKLVNTFDARGFVKFRNLAKLSRP-------KRRK 1850
            LVSL RRI+GK++ DG+ LSPVLYRD+L  TF+  GFVKFR+LAK+SRP       KR++
Sbjct: 148  LVSLQRRIHGKADGDGDVLSPVLYRDRLARTFNTSGFVKFRHLAKISRPNRNKRRRKRKE 207

Query: 1849 KQDIVGEKQVREKKGGEVYAXXXXXXXXXXXXXXXXXXXXDFSGRKWRGSTRLLLLDERY 1670
            K+   G K+V E  G                         +F+ +KWRGSTRLLLLDERY
Sbjct: 208  KESSEGVKRVGEN-GFAAVEVIEEEEEGEESGGLRNLLGEEFARKKWRGSTRLLLLDERY 266

Query: 1669 GDKELDEMPEAIKAVFNKGGSESMTSAIELVRCNLTLFYTYWQMNEILEALLPAGLIIPS 1490
             D+  +E+PEA KAV  +   +S    +ELVRC LTLFY YWQMNE+LE LLP G+IIPS
Sbjct: 267  ADRGFEELPEATKAVLKEYAEKSTNLMLELVRCKLTLFYDYWQMNEVLETLLPEGMIIPS 326

Query: 1489 AFETVGHIAHLNLRDEHHSHKKLIAKVVLDKNKPKIQTVVNKIDAIHNDYRTMQLEVLAG 1310
            AFETVGHIAHLNLRDEH  +K LIAKVVLDKNKP+IQTVVNKID I N+YRTMQLEVLAG
Sbjct: 327  AFETVGHIAHLNLRDEHLPYKWLIAKVVLDKNKPRIQTVVNKIDTIQNEYRTMQLEVLAG 386

Query: 1309 NHSLVTTVVENGLRFHVDLATVYWNSKLATERQRLVNCFTPSDIVCDVFAGVGPIAIAAA 1130
            NHSLVTTVVENG+ F VDLATVYW+S+L TERQRL++ FT +D+VCDVF+GVGP+AI+AA
Sbjct: 387  NHSLVTTVVENGIHFQVDLATVYWSSRLGTERQRLLSGFTRNDVVCDVFSGVGPLAISAA 446

Query: 1129 KRVKHVYANDLNPDAVDFLQRNCVLNKLERKIEVFNMDGRRFIDSVFARLKARCITQVVM 950
            + VK V+ANDLNP AV++L+RNCVLNKL+RKI+VFNMDGRRFI S++    ++ ITQVVM
Sbjct: 447  RIVKRVFANDLNPYAVEYLERNCVLNKLDRKIKVFNMDGRRFIKSMYDSDISQSITQVVM 506

Query: 949  NLPNDAAEYLDVFRGLFGDYNTAKTLTLPKVHVYGFSKAQDPEFEFQERIRIALSEVAFE 770
            NLP++AAE+LD FRG++ +       TLP +HVYGFSKA+DPEF+F ERIRIAL EVA  
Sbjct: 507  NLPSEAAEFLDAFRGIYKNRPKDGEYTLPLIHVYGFSKARDPEFDFHERIRIALLEVAVN 566

Query: 769  IQMHRVRLVAPGKWMMCASFVLPERVAF 686
            + M RVRLVAPGKWM+CASF+LP+ VAF
Sbjct: 567  VDMRRVRLVAPGKWMLCASFLLPKSVAF 594