BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00018239
         (1254 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003555526.1| PREDICTED: pyruvate kinase isozyme A, chloro...   515   e-143
ref|XP_002315744.1| predicted protein [Populus trichocarpa] gi|2...   514   e-143
ref|XP_002517044.1| pyruvate kinase, putative [Ricinus communis]...   514   e-143
sp|Q40545.1|KPYA_TOBAC RecName: Full=Pyruvate kinase isozyme A, ...   513   e-143
ref|XP_002311649.1| predicted protein [Populus trichocarpa] gi|2...   510   e-142

>ref|XP_003555526.1| PREDICTED: pyruvate kinase isozyme A, chloroplastic-like [Glycine
            max]
          Length = 655

 Score =  515 bits (1326), Expect = e-143
 Identities = 256/363 (70%), Positives = 308/363 (84%), Gaps = 7/363 (1%)
 Frame = +2

Query: 185  TTPTLLVS--NGGASQPPL--STIEV---VDKEIDVESVNGYRKTKIVCTIGPSTCGFDE 343
            ++P +LVS  NG  S PP   S+IEV    + E+        R+TK+VCT+GP+TCGFD+
Sbjct: 134  SSPEILVSDNNGAPSPPPSDPSSIEVDAVTEAELKENGFRSTRRTKLVCTVGPATCGFDQ 193

Query: 344  LEALAAGGMNVARLNMCHATHQWHRNVIRNLRKLNRDRGFCVSIMIDTQGNQIHMGDLAS 523
            LEALA GGMNVAR+NMCH T +WH+ VI  +R+LN ++GF V+IM+DT+G++IHMGDL  
Sbjct: 194  LEALAVGGMNVARINMCHGTREWHKEVIDRVRRLNHEKGFAVAIMMDTEGSEIHMGDLGG 253

Query: 524  ASSSAKVDHGDVWTFSVRSYDSDELPESTVTVNYDGFAEDVCVGDELIVDGGMARFEVVY 703
            ASS AK D G++WTFSVR++DS  LP+ T+ VNY+GFAEDV VGDEL+VDGGM RFEV+ 
Sbjct: 254  ASS-AKADDGEIWTFSVRAFDS-ALPQRTINVNYEGFAEDVKVGDELLVDGGMVRFEVIQ 311

Query: 704  KIGPDVKCLCTDPGLLLPRANLTFWRDGKFVQERNAMLPTISAKDWLDIDFGISEGVDFI 883
            KIGPDVKC CTDPGLLLPRANLTFWR+G  V+ERNAMLPTIS+KDWLDIDFGISEGVDFI
Sbjct: 312  KIGPDVKCRCTDPGLLLPRANLTFWRNGSLVRERNAMLPTISSKDWLDIDFGISEGVDFI 371

Query: 884  AVSFVKSAEVINHLKTYLAAHARNSKISVISKIENVDSLKNLDTIIEASDGVMVARGDLG 1063
            A+SFVKSAEVI HLK+Y+AA +R+S ISVI+KIE++DSLKNL+ I+ A+DG MVARGDLG
Sbjct: 372  AISFVKSAEVITHLKSYIAARSRDSDISVIAKIESIDSLKNLEEIVLAADGAMVARGDLG 431

Query: 1064 AHIPLEQVPSVQQKIVEMCRELNKPVIVASQLLESMIEYPIPTRAEVADVTEVVRQQADA 1243
            A IPLEQVPS QQ+IV++CR+LNKPVIVASQLLESMIEYP PTRAEVADV+E VRQ+ADA
Sbjct: 432  AQIPLEQVPSAQQRIVQVCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRADA 491

Query: 1244 LML 1252
            LML
Sbjct: 492  LML 494


>ref|XP_002315744.1| predicted protein [Populus trichocarpa] gi|222864784|gb|EEF01915.1|
            predicted protein [Populus trichocarpa]
          Length = 590

 Score =  514 bits (1324), Expect = e-143
 Identities = 259/379 (68%), Positives = 309/379 (81%), Gaps = 23/379 (6%)
 Frame = +2

Query: 185  TTPTLLVSNGGASQ-------------PPLSTIEVVDKEIDVESV-------NGYR---K 295
            T+P +L+SN G                PP  +I      I+V++V       NG+R   +
Sbjct: 53   TSPQVLISNNGTGASGILSSTPQDYDTPPSQSIISDSSSIEVDAVTEVELKENGFRSTRR 112

Query: 296  TKIVCTIGPSTCGFDELEALAAGGMNVARLNMCHATHQWHRNVIRNLRKLNRDRGFCVSI 475
            TK+VCTIGP+TCGF+ELEALA GGMNVAR+NMCH T +WHR VI  +R+LN ++GF V+I
Sbjct: 113  TKLVCTIGPATCGFEELEALAVGGMNVARINMCHGTREWHRRVIERVRRLNEEKGFAVAI 172

Query: 476  MIDTQGNQIHMGDLASASSSAKVDHGDVWTFSVRSYDSDELPESTVTVNYDGFAEDVCVG 655
            M+DT+G++IHMGDL  ASS AK + G++WTFSVR++DS   PE TV VNYDGFAEDV VG
Sbjct: 173  MMDTEGSEIHMGDLGGASS-AKAEDGEIWTFSVRAFDSHR-PERTVNVNYDGFAEDVKVG 230

Query: 656  DELIVDGGMARFEVVYKIGPDVKCLCTDPGLLLPRANLTFWRDGKFVQERNAMLPTISAK 835
            DEL+VDGGM RFEV+ KIGPDVKC CTDPGL+LPRAN+TFWRDG  V+ERNAMLPTIS+K
Sbjct: 231  DELLVDGGMVRFEVMEKIGPDVKCRCTDPGLMLPRANVTFWRDGSLVRERNAMLPTISSK 290

Query: 836  DWLDIDFGISEGVDFIAVSFVKSAEVINHLKTYLAAHARNSKISVISKIENVDSLKNLDT 1015
            DWLDIDFGISEGVDFIA+SFVKSAEVI HLK+Y+AA +R+S I+VI+KIE++DSLKNL+ 
Sbjct: 291  DWLDIDFGISEGVDFIAISFVKSAEVITHLKSYIAARSRDSDIAVIAKIESIDSLKNLEE 350

Query: 1016 IIEASDGVMVARGDLGAHIPLEQVPSVQQKIVEMCRELNKPVIVASQLLESMIEYPIPTR 1195
            II+ASDG MVARGDLGA IPLEQVPS QQ IV++CR+LNKPVIVASQLLESMIEYP PTR
Sbjct: 351  IIQASDGAMVARGDLGAQIPLEQVPSAQQNIVQICRQLNKPVIVASQLLESMIEYPTPTR 410

Query: 1196 AEVADVTEVVRQQADALML 1252
            AEVADV+E VRQ+ADALML
Sbjct: 411  AEVADVSEAVRQRADALML 429


>ref|XP_002517044.1| pyruvate kinase, putative [Ricinus communis]
            gi|2497539|sp|Q43117.1|KPYA_RICCO RecName: Full=Pyruvate
            kinase isozyme A, chloroplastic; Flags: Precursor
            gi|169703|gb|AAA33870.1| ATP:pyruvate phosphotransferase
            [Ricinus communis] gi|223543679|gb|EEF45207.1| pyruvate
            kinase, putative [Ricinus communis]
          Length = 583

 Score =  514 bits (1323), Expect = e-143
 Identities = 260/383 (67%), Positives = 314/383 (81%), Gaps = 17/383 (4%)
 Frame = +2

Query: 155  IKVCSIRSEITTPTLLVSNGGASQPPL------STIEVVD-KEIDVESV-------NGYR 292
            IK  +  S  + P +LV++ G     +       ++ V D   I+V++V       NG+R
Sbjct: 42   IKASTSPSSSSDPQVLVADNGTGNSGVLYNNNNKSVTVSDPSSIEVDAVTETELKENGFR 101

Query: 293  ---KTKIVCTIGPSTCGFDELEALAAGGMNVARLNMCHATHQWHRNVIRNLRKLNRDRGF 463
               +TK+VCTIGP+TCGF+ELEALA GGMNVAR+NMCH T +WH++VI  +R+LN ++GF
Sbjct: 102  STRRTKLVCTIGPATCGFEELEALAVGGMNVARINMCHGTREWHKSVIERVRRLNEEKGF 161

Query: 464  CVSIMIDTQGNQIHMGDLASASSSAKVDHGDVWTFSVRSYDSDELPESTVTVNYDGFAED 643
             V+IM+DT+G++IHMGDL  ASS AK + G++WTFSVR+YDS   PE T+ VNYDGFAED
Sbjct: 162  AVAIMMDTEGSEIHMGDLGGASS-AKAEDGEIWTFSVRAYDSPR-PERTINVNYDGFAED 219

Query: 644  VCVGDELIVDGGMARFEVVYKIGPDVKCLCTDPGLLLPRANLTFWRDGKFVQERNAMLPT 823
            V VGDEL+VDGGM RFEV+ KIGPDVKC CTDPGLLLPRANLTFWRDG  V+ERNAMLPT
Sbjct: 220  VKVGDELLVDGGMVRFEVIEKIGPDVKCRCTDPGLLLPRANLTFWRDGSLVRERNAMLPT 279

Query: 824  ISAKDWLDIDFGISEGVDFIAVSFVKSAEVINHLKTYLAAHARNSKISVISKIENVDSLK 1003
            IS+KDWLDIDFGI+EGVDFIA+SFVKSAEVINHLK+Y+AA +R+S I+VI+KIE++DSLK
Sbjct: 280  ISSKDWLDIDFGIAEGVDFIAISFVKSAEVINHLKSYIAARSRDSDIAVIAKIESIDSLK 339

Query: 1004 NLDTIIEASDGVMVARGDLGAHIPLEQVPSVQQKIVEMCRELNKPVIVASQLLESMIEYP 1183
            NL+ II ASDG MVARGDLGA IPLEQVPS QQ IV++CR+LNKPVIVASQLLESMIEYP
Sbjct: 340  NLEEIIRASDGAMVARGDLGAQIPLEQVPSAQQNIVQVCRQLNKPVIVASQLLESMIEYP 399

Query: 1184 IPTRAEVADVTEVVRQQADALML 1252
             PTRAEVADV+E VRQ+ADALML
Sbjct: 400  TPTRAEVADVSEAVRQRADALML 422


>sp|Q40545.1|KPYA_TOBAC RecName: Full=Pyruvate kinase isozyme A, chloroplastic; Flags:
            Precursor gi|482936|emb|CAA82222.1| pyruvate kinase;
            plastid isozyme [Nicotiana tabacum]
          Length = 593

 Score =  513 bits (1320), Expect = e-143
 Identities = 251/346 (72%), Positives = 294/346 (84%)
 Frame = +2

Query: 215  GASQPPLSTIEVVDKEIDVESVNGYRKTKIVCTIGPSTCGFDELEALAAGGMNVARLNMC 394
            G     +    V + E+        R+TK++CTIGP+TCGF++LE LA GGMNVAR+NMC
Sbjct: 89   GTDSSSIEVDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLERLAEGGMNVARINMC 148

Query: 395  HATHQWHRNVIRNLRKLNRDRGFCVSIMIDTQGNQIHMGDLASASSSAKVDHGDVWTFSV 574
            H T +WHR VI  LR+LN ++GF V+IM+DT+G++IHMGDL  ASS AK + G++W F+V
Sbjct: 149  HGTREWHRMVIERLRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASS-AKAEDGEIWNFTV 207

Query: 575  RSYDSDELPESTVTVNYDGFAEDVCVGDELIVDGGMARFEVVYKIGPDVKCLCTDPGLLL 754
            RS+D   LPE TVTVNYDGFAEDV VGDEL+VDGGM RFEV+ KIGPDVKCLCTDPGLLL
Sbjct: 208  RSFDPP-LPERTVTVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLL 266

Query: 755  PRANLTFWRDGKFVQERNAMLPTISAKDWLDIDFGISEGVDFIAVSFVKSAEVINHLKTY 934
            PRANLTFWRDGK V+ERNAMLPTIS+KDWLDIDFGI+EGVDFIAVSFVKSAEVI HLK+Y
Sbjct: 267  PRANLTFWRDGKLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVIKHLKSY 326

Query: 935  LAAHARNSKISVISKIENVDSLKNLDTIIEASDGVMVARGDLGAHIPLEQVPSVQQKIVE 1114
            + A AR+S ISVI+KIE++DSLKNL+ II+ASDG MVARGDLGA IPLEQVPS QQKIV+
Sbjct: 327  IQARARDSDISVIAKIESIDSLKNLEEIIQASDGAMVARGDLGAQIPLEQVPSEQQKIVQ 386

Query: 1115 MCRELNKPVIVASQLLESMIEYPIPTRAEVADVTEVVRQQADALML 1252
            +CR+LN+PVIVASQLLESMIEYPIPTRAEVADV+E VRQ+ DALML
Sbjct: 387  ICRQLNRPVIVASQLLESMIEYPIPTRAEVADVSEAVRQRGDALML 432


>ref|XP_002311649.1| predicted protein [Populus trichocarpa] gi|222851469|gb|EEE89016.1|
            predicted protein [Populus trichocarpa]
          Length = 591

 Score =  510 bits (1313), Expect = e-142
 Identities = 255/382 (66%), Positives = 308/382 (80%), Gaps = 23/382 (6%)
 Frame = +2

Query: 176  SEITTPTLLVSNGGASQ-------------PPLSTIEVVDKEIDVESV-------NGYR- 292
            S  T P +L+S+ G                PP  +       IDV++        NG+R 
Sbjct: 51   SSTTPPQVLISDNGTGAGEILSSTPQDYDTPPSQSFLSDSSSIDVDAATEVELKENGFRS 110

Query: 293  --KTKIVCTIGPSTCGFDELEALAAGGMNVARLNMCHATHQWHRNVIRNLRKLNRDRGFC 466
              +TK+VCTIGP+TCGF+EL+ALA GGMNVAR+NMCH T +WH+ VI  +R+LN ++GF 
Sbjct: 111  TRRTKLVCTIGPATCGFEELQALAVGGMNVARINMCHGTREWHKRVIERVRRLNEEKGFA 170

Query: 467  VSIMIDTQGNQIHMGDLASASSSAKVDHGDVWTFSVRSYDSDELPESTVTVNYDGFAEDV 646
            V+IM+DT+G++IHMGDL  ASS AK + G++WTFSVR++D    PE T+ VNYDGFAEDV
Sbjct: 171  VAIMMDTEGSEIHMGDLGGASS-AKAEDGEIWTFSVRAFDLPR-PERTINVNYDGFAEDV 228

Query: 647  CVGDELIVDGGMARFEVVYKIGPDVKCLCTDPGLLLPRANLTFWRDGKFVQERNAMLPTI 826
             VGDEL+VDGGM RFEV+ KIGPDVKC CTDPGL+LPRANLTFWRDG  V+ERNAMLPTI
Sbjct: 229  KVGDELLVDGGMVRFEVIEKIGPDVKCRCTDPGLMLPRANLTFWRDGSLVRERNAMLPTI 288

Query: 827  SAKDWLDIDFGISEGVDFIAVSFVKSAEVINHLKTYLAAHARNSKISVISKIENVDSLKN 1006
            S+KDWLDIDFGI+EGVDFIA+SFVKSAEVINHLK+Y+AA +R+S I+VI+KIE++DSL+N
Sbjct: 289  SSKDWLDIDFGIAEGVDFIAISFVKSAEVINHLKSYIAARSRDSDIAVIAKIESIDSLRN 348

Query: 1007 LDTIIEASDGVMVARGDLGAHIPLEQVPSVQQKIVEMCRELNKPVIVASQLLESMIEYPI 1186
            L+ II+ASDG MVARGDLGA IPLEQVPS QQKIV++CR+LNKPVIVASQLLESMIEYP 
Sbjct: 349  LEEIIQASDGAMVARGDLGAQIPLEQVPSAQQKIVQICRQLNKPVIVASQLLESMIEYPT 408

Query: 1187 PTRAEVADVTEVVRQQADALML 1252
            PTRAEVADV+E VRQ+ADALML
Sbjct: 409  PTRAEVADVSEAVRQRADALML 430