BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00018201
         (1943 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002263801.1| PREDICTED: putative acyl-CoA synthetase YngI...   787   0.0  
ref|XP_002521743.1| AMP dependent ligase, putative [Ricinus comm...   783   0.0  
ref|XP_002263877.2| PREDICTED: medium-chain-fatty-acid--CoA liga...   781   0.0  
emb|CAN64147.1| hypothetical protein VITISV_042774 [Vitis vinifera]   766   0.0  
ref|XP_002321934.1| predicted protein [Populus trichocarpa] gi|2...   758   0.0  

>ref|XP_002263801.1| PREDICTED: putative acyl-CoA synthetase YngI-like [Vitis vinifera]
          Length = 549

 Score =  787 bits (2032), Expect = 0.0
 Identities = 377/554 (68%), Positives = 451/554 (81%)
 Frame = -3

Query: 1797 MDQFKPTRANSCPLTPLTFLERAATVFGDDPSVVFNSRVFTWSETKERCFRLASSIVSLG 1618
            MD  KP  ANS PLTPL FL+RAATV+GD PSV++NS  +TWS+T  RC ++ASSI S G
Sbjct: 1    MDSLKPRPANSSPLTPLGFLDRAATVYGDCPSVLYNSITYTWSQTHRRCLQVASSIKSFG 60

Query: 1617 IQRGQVVTVLAPNIPAMYELQFAVPMAGAVINNVNTRLDARSVAVILCHAESKLVFVCAQ 1438
            I RG VV+V+APN+PAMYEL FAVPM+GAV+N +NTRLDAR+V+V+L H+ESKLVFV   
Sbjct: 61   IGRGHVVSVVAPNVPAMYELHFAVPMSGAVLNTINTRLDARTVSVLLRHSESKLVFVDCL 120

Query: 1437 YCHILLEAISMLPPDFQHPSLVIINAGDYVSLSPPDLSNFLCSHEDMVERGDPRFEWIRP 1258
               ++LEA+S+ PP+ Q P LV+I   +   ++P    +F+C++ED+VERGDP F+W+RP
Sbjct: 121  SRALILEALSLFPPNTQWPLLVLIADEE---VAPSSTVDFICTYEDLVERGDPEFKWVRP 177

Query: 1257 ENEWQPMTLNYTSGTTSAPKGVVHSHRSIFIITVDSLIDWSVPKQPVYLWTLPMFHSNGW 1078
            E+EW P+ LNYTSGTTS+PKGVVH HR IFI+T+DSL++WSVPKQPVYLWTLPMFH NGW
Sbjct: 178  ESEWDPIILNYTSGTTSSPKGVVHCHRGIFIVTIDSLVEWSVPKQPVYLWTLPMFHGNGW 237

Query: 1077 SFTWGMAAVGGTNICLRKFDATLIHTAIPKYGVTHMCGAPVVLNMLSNSPKAKKLKKPVH 898
            +FTWGMAA+GGTN+CLRKFDA +I+ AIPKYG+THMC APVVLNMLSN P  + L  PV 
Sbjct: 238  TFTWGMAAIGGTNVCLRKFDARIIYDAIPKYGITHMCAAPVVLNMLSNYPNHQPLGDPVK 297

Query: 897  FLTGGAPPPAAVLLRTEALGFIVSHGFGMTEVAGVVVSCAWKRHWNVLPALERARLKARQ 718
             LT GAPPP +VLLRTE+LGF+VSHG+GMTE AGVVVSCAWK  W+  PA ERAR+KARQ
Sbjct: 298  ILTAGAPPPYSVLLRTESLGFVVSHGYGMTETAGVVVSCAWKPQWDRFPASERARVKARQ 357

Query: 717  GVRTIGMTEVNIVDPKSGIIVKPDGLTQGEIVLRGGCLMLGYLKDPEGTAKCMKNDGWLY 538
            GVRT+ MTEV++VDPKSG+ VK DG + GEIVLRG C+MLGYLKDPEGT+KCM  +GWLY
Sbjct: 358  GVRTVVMTEVDVVDPKSGVSVKRDGSSLGEIVLRGACIMLGYLKDPEGTSKCMSKEGWLY 417

Query: 537  TGDVGVMHPDGYLEIKDRSKDVIIXXXXXXXXXXXXXVLYLNPVVNEAAVVARPDEFWGE 358
            TGDV VMHPDGY+EIKDRSKDVII             VLY +P VNEAAVVARPD+FWGE
Sbjct: 418  TGDVAVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYTHPAVNEAAVVARPDDFWGE 477

Query: 357  TPCAFVSLKVAAGMIENAKEKEIMEFCRDRLPHYMVPKTVVFMGELPKNATGKIQKFSLR 178
            TPCAFVSLK  +   +    KE+ME+CR R+PHYMVPKTVVF  ELPK +TGKIQKF LR
Sbjct: 478  TPCAFVSLKPESP--DKPTAKEMMEYCRARMPHYMVPKTVVFKEELPKTSTGKIQKFKLR 535

Query: 177  DIAKSLGNSPASRL 136
            DIAK++G SPASR+
Sbjct: 536  DIAKAMGCSPASRM 549


>ref|XP_002521743.1| AMP dependent ligase, putative [Ricinus communis]
            gi|223538956|gb|EEF40553.1| AMP dependent ligase,
            putative [Ricinus communis]
          Length = 556

 Score =  783 bits (2023), Expect = 0.0
 Identities = 372/558 (66%), Positives = 454/558 (81%), Gaps = 4/558 (0%)
 Frame = -3

Query: 1797 MDQFKPTRANSCPLTPLTFLERAATVFGDDPSVVFNSRVFTWSETKERCFRLASSIVSLG 1618
            MD  KP  ANS PLTPL+FLERA+TV+GD PS+V+N+  +TWS+T  RC +LASS+ S G
Sbjct: 1    MDHLKPKPANSFPLTPLSFLERASTVYGDCPSIVYNTATYTWSQTHRRCLQLASSLSSGG 60

Query: 1617 IQRGQVVTVLAPNIPAMYELQFAVPMAGAVINNVNTRLDARSVAVILCHAESKLVFVCAQ 1438
              RG VV+V+APN P MYELQFAVPM+GAV+NN+NTRLDAR+V+V+L H++SKLVFV   
Sbjct: 61   FTRGDVVSVVAPNTPPMYELQFAVPMSGAVLNNINTRLDARTVSVLLRHSQSKLVFVDCL 120

Query: 1437 YCHILLEAISMLPPDFQHPSLVIINAGDYV----SLSPPDLSNFLCSHEDMVERGDPRFE 1270
               ++LEA+S+ P   + P+LV+I   D      S S     +F C++E MVE+GDP F+
Sbjct: 121  SRDLVLEAVSLFPSSVKRPTLVLITDDDEAPASSSTSSSSTVDFCCTYESMVEKGDPEFK 180

Query: 1269 WIRPENEWQPMTLNYTSGTTSAPKGVVHSHRSIFIITVDSLIDWSVPKQPVYLWTLPMFH 1090
            W++P++EW PM LNYTSGTTS+PKGVVH HR I+IITV+SLIDW+VP+QPVYLWTLP+FH
Sbjct: 181  WVQPQSEWDPMILNYTSGTTSSPKGVVHCHRGIYIITVESLIDWNVPRQPVYLWTLPLFH 240

Query: 1089 SNGWSFTWGMAAVGGTNICLRKFDATLIHTAIPKYGVTHMCGAPVVLNMLSNSPKAKKLK 910
            +NGWSF WGMAAVGGTNIC+RKFDA+ I+  I ++GVTHMCGAPVVLNML+NSP  + LK
Sbjct: 241  ANGWSFPWGMAAVGGTNICIRKFDASSIYGLIKRHGVTHMCGAPVVLNMLTNSPDNEPLK 300

Query: 909  KPVHFLTGGAPPPAAVLLRTEALGFIVSHGFGMTEVAGVVVSCAWKRHWNVLPALERARL 730
            +PVH LT GAPPPAAVL RTE+LGF+VSHG+G+TE AG+VVSCAWK  WN +PA ERARL
Sbjct: 301  RPVHILTAGAPPPAAVLFRTESLGFVVSHGYGLTETAGLVVSCAWKPKWNTVPASERARL 360

Query: 729  KARQGVRTIGMTEVNIVDPKSGIIVKPDGLTQGEIVLRGGCLMLGYLKDPEGTAKCMKND 550
            KARQGVR  G TE++++DPK+G+ VK DGL+ GEIVLRGGC+M+GYLKDP+GT+KC+K+D
Sbjct: 361  KARQGVRIAGFTEIDVIDPKTGLSVKRDGLSLGEIVLRGGCVMMGYLKDPDGTSKCLKDD 420

Query: 549  GWLYTGDVGVMHPDGYLEIKDRSKDVIIXXXXXXXXXXXXXVLYLNPVVNEAAVVARPDE 370
            GW YTGDVGVMHPDGYLEIKDRSKDVII             VLY NPVVNEAAVVARPDE
Sbjct: 421  GWFYTGDVGVMHPDGYLEIKDRSKDVIISGGENISSVEVESVLYSNPVVNEAAVVARPDE 480

Query: 369  FWGETPCAFVSLKVAAGMIENAKEKEIMEFCRDRLPHYMVPKTVVFMGELPKNATGKIQK 190
            +WGETPCAFVSL+   G  E   EK+I+E+CR+++PHYMVPKTVVF  ELPK +TGKIQK
Sbjct: 481  YWGETPCAFVSLR--DGAREKVSEKDIIEYCREKMPHYMVPKTVVFKEELPKTSTGKIQK 538

Query: 189  FSLRDIAKSLGNSPASRL 136
            F LRDIAK++G S  SR+
Sbjct: 539  FVLRDIAKAMGASKVSRM 556


>ref|XP_002263877.2| PREDICTED: medium-chain-fatty-acid--CoA ligase [Vitis vinifera]
          Length = 588

 Score =  781 bits (2016), Expect = 0.0
 Identities = 380/554 (68%), Positives = 450/554 (81%)
 Frame = -3

Query: 1797 MDQFKPTRANSCPLTPLTFLERAATVFGDDPSVVFNSRVFTWSETKERCFRLASSIVSLG 1618
            M++ KP  ANS PLT L+FL+RAATV+GD PSVV+NS  +TWS+T  RC ++ASSI SLG
Sbjct: 40   MEELKPRPANSSPLTTLSFLDRAATVYGDCPSVVYNSTTYTWSQTHHRCLQVASSIKSLG 99

Query: 1617 IQRGQVVTVLAPNIPAMYELQFAVPMAGAVINNVNTRLDARSVAVILCHAESKLVFVCAQ 1438
            I RGQVV+V+APNIPAMYEL FAVPMAGAV+N +NTRLDA++V+V+L H+ESKL+FV  Q
Sbjct: 100  IGRGQVVSVVAPNIPAMYELHFAVPMAGAVLNTINTRLDAKTVSVLLRHSESKLIFVDYQ 159

Query: 1437 YCHILLEAISMLPPDFQHPSLVIINAGDYVSLSPPDLSNFLCSHEDMVERGDPRFEWIRP 1258
               ++LE++S+ PP  Q P LV+I   +    SPP   +FLC++EDMV RGDP F+WIRP
Sbjct: 160  SRPLILESLSLFPPSSQRPLLVLITDDED---SPPSTVDFLCTYEDMVGRGDPEFKWIRP 216

Query: 1257 ENEWQPMTLNYTSGTTSAPKGVVHSHRSIFIITVDSLIDWSVPKQPVYLWTLPMFHSNGW 1078
            E+EW PM LNYTSGTTS+PKGVVH HR IFI+TVDSLIDW VPKQPVYLWTLPMFH+NGW
Sbjct: 217  ESEWNPMILNYTSGTTSSPKGVVHCHRGIFIVTVDSLIDWCVPKQPVYLWTLPMFHANGW 276

Query: 1077 SFTWGMAAVGGTNICLRKFDATLIHTAIPKYGVTHMCGAPVVLNMLSNSPKAKKLKKPVH 898
            SF WGMAAVGGTN+CLRKFDA++I+ AI  + VTHMCGAPVVLNMLSN P  + LK PV 
Sbjct: 277  SFPWGMAAVGGTNVCLRKFDASVIYEAIRVHRVTHMCGAPVVLNMLSNWPGTEPLKNPVQ 336

Query: 897  FLTGGAPPPAAVLLRTEALGFIVSHGFGMTEVAGVVVSCAWKRHWNVLPALERARLKARQ 718
             LT GAPPPAA+LLRTE+ GFIVSHG+G+TE  G+VVSC WK+ WN LPA ERARLK+RQ
Sbjct: 337  ILTAGAPPPAAILLRTESRGFIVSHGYGLTETGGLVVSCVWKQKWNRLPATERARLKSRQ 396

Query: 717  GVRTIGMTEVNIVDPKSGIIVKPDGLTQGEIVLRGGCLMLGYLKDPEGTAKCMKNDGWLY 538
            GVRT+GMTEV+++DP SG+ VK DG + GEIVLRGGC+MLGYLKDP+GT+K + +DGW +
Sbjct: 397  GVRTVGMTEVDVLDPGSGLSVKRDGSSLGEIVLRGGCIMLGYLKDPKGTSKSLTDDGWFF 456

Query: 537  TGDVGVMHPDGYLEIKDRSKDVIIXXXXXXXXXXXXXVLYLNPVVNEAAVVARPDEFWGE 358
            TGDVGVMH DGYLEIKDRSKDVII             VLY +P VNEAAVVARPD+FWGE
Sbjct: 457  TGDVGVMHSDGYLEIKDRSKDVIISGGENLSSVEVESVLYTHPAVNEAAVVARPDDFWGE 516

Query: 357  TPCAFVSLKVAAGMIENAKEKEIMEFCRDRLPHYMVPKTVVFMGELPKNATGKIQKFSLR 178
            TPCAFVSLK      E   EKEIME+CR R+PHYMVPKTVVF  ELPK +TGKIQKF LR
Sbjct: 517  TPCAFVSLK--PERTEQPTEKEIMEYCRARMPHYMVPKTVVFEEELPKTSTGKIQKFVLR 574

Query: 177  DIAKSLGNSPASRL 136
            + AK++G+S  SR+
Sbjct: 575  ETAKAMGSSRVSRM 588


>emb|CAN64147.1| hypothetical protein VITISV_042774 [Vitis vinifera]
          Length = 537

 Score =  766 bits (1977), Expect = 0.0
 Identities = 375/554 (67%), Positives = 441/554 (79%)
 Frame = -3

Query: 1797 MDQFKPTRANSCPLTPLTFLERAATVFGDDPSVVFNSRVFTWSETKERCFRLASSIVSLG 1618
            MD  KP  ANS PLTPL FL+RAATV+GD PSV++NS  +TWS+T  RC ++ASSI S G
Sbjct: 1    MDSLKPRPANSSPLTPLGFLDRAATVYGDCPSVLYNSITYTWSQTHRRCLQVASSIKSFG 60

Query: 1617 IQRGQVVTVLAPNIPAMYELQFAVPMAGAVINNVNTRLDARSVAVILCHAESKLVFVCAQ 1438
            I RG VV+V+APN+PAMYEL FAVPM+GAV+N +NTRLDAR+V+V+L H+ESKLVFV   
Sbjct: 61   IGRGHVVSVVAPNVPAMYELHFAVPMSGAVLNTINTRLDARTVSVLLRHSESKLVFVDCL 120

Query: 1437 YCHILLEAISMLPPDFQHPSLVIINAGDYVSLSPPDLSNFLCSHEDMVERGDPRFEWIRP 1258
               ++LEA+S+ PP+ Q P LV+I A + V+ S                RGDP F+W+RP
Sbjct: 121  SRALILEALSLFPPNTQWPLLVLI-ADEEVAPSST--------------RGDPEFKWVRP 165

Query: 1257 ENEWQPMTLNYTSGTTSAPKGVVHSHRSIFIITVDSLIDWSVPKQPVYLWTLPMFHSNGW 1078
            E+EW P+ LNYTSGTTS+PKGVVH HR IFI+T+DSL++WSVPKQPVYLWTLPMFH NGW
Sbjct: 166  ESEWDPIILNYTSGTTSSPKGVVHCHRGIFIVTIDSLVEWSVPKQPVYLWTLPMFHGNGW 225

Query: 1077 SFTWGMAAVGGTNICLRKFDATLIHTAIPKYGVTHMCGAPVVLNMLSNSPKAKKLKKPVH 898
            +FTWGMAAVGGTN+CLRKFDA +I+ AIPKYG+THMC APVVLNMLSN P  + L  PV 
Sbjct: 226  TFTWGMAAVGGTNVCLRKFDARIIYDAIPKYGITHMCAAPVVLNMLSNYPNHQPLGYPVK 285

Query: 897  FLTGGAPPPAAVLLRTEALGFIVSHGFGMTEVAGVVVSCAWKRHWNVLPALERARLKARQ 718
             LT GAPPP +VLLRTE+LGF+VSHG+GMTE AGVVVSCAWK  W+  PA ERAR+KARQ
Sbjct: 286  ILTAGAPPPYSVLLRTESLGFVVSHGYGMTETAGVVVSCAWKPQWDRFPASERARVKARQ 345

Query: 717  GVRTIGMTEVNIVDPKSGIIVKPDGLTQGEIVLRGGCLMLGYLKDPEGTAKCMKNDGWLY 538
            GVRT+ MTEV++VDPKSG+ VK DG + GEIVLRG C+MLGYLKDPEGT+KCM  +GWLY
Sbjct: 346  GVRTVVMTEVDVVDPKSGVSVKRDGSSLGEIVLRGACIMLGYLKDPEGTSKCMSKEGWLY 405

Query: 537  TGDVGVMHPDGYLEIKDRSKDVIIXXXXXXXXXXXXXVLYLNPVVNEAAVVARPDEFWGE 358
            TGDV VMHPDGY+EIKDRSKDVII             VLY +P VNEAAVVARPD+FWGE
Sbjct: 406  TGDVAVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYTHPAVNEAAVVARPDDFWGE 465

Query: 357  TPCAFVSLKVAAGMIENAKEKEIMEFCRDRLPHYMVPKTVVFMGELPKNATGKIQKFSLR 178
            TPCAFVSLK   G  +    KE+ME+CR R+PHYMVPKTVVF  ELPK +TGKIQKF LR
Sbjct: 466  TPCAFVSLK--PGSPDKPTAKEMMEYCRARMPHYMVPKTVVFKEELPKTSTGKIQKFKLR 523

Query: 177  DIAKSLGNSPASRL 136
            DIAK++G SPASR+
Sbjct: 524  DIAKAMGCSPASRM 537


>ref|XP_002321934.1| predicted protein [Populus trichocarpa] gi|222868930|gb|EEF06061.1|
            predicted protein [Populus trichocarpa]
          Length = 553

 Score =  758 bits (1956), Expect = 0.0
 Identities = 364/555 (65%), Positives = 439/555 (79%), Gaps = 1/555 (0%)
 Frame = -3

Query: 1797 MDQFKPTRANSCPLTPLTFLERAATVFGDDPSVVFNSRVFTWSETKERCFRLASSIVSLG 1618
            MD+ +P   NS  LTP+ FL+RAATV+GD PS+++N   +TWS+T  RC +LASS+ S+G
Sbjct: 1    MDELRPRPPNSYALTPVGFLDRAATVYGDCPSIIYNDISYTWSQTHSRCLQLASSLSSIG 60

Query: 1617 IQRGQVVTVLAPNIPAMYELQFAVPMAGAVINNVNTRLDARSVAVILCHAESKLVFVCAQ 1438
            I +G VV+V+APNIPAMYEL FAVPMAGA++N +NTRLDAR+++++LCHAESKL+FV   
Sbjct: 61   INKGHVVSVIAPNIPAMYELHFAVPMAGAILNTLNTRLDARTISLLLCHAESKLLFVDPM 120

Query: 1437 YCHILLEAISMLPPDFQHPSLVIINAGDYVSLSPPDLSNFLCSHEDMVERGDPRFEWIRP 1258
            Y  ++ EAIS+ PP+ +HP LV+I   + VS       +F  ++E +VE+GDP F WIRP
Sbjct: 121  YVSLVHEAISLFPPNTKHPPLVLIADDEVVSQQSSPTIHFYDTYESLVEKGDPAFNWIRP 180

Query: 1257 ENEWQPMTLNYTSGTTSAPKGVVHSHRSIFIITVDSLIDWSVPKQPVYLWTLPMFHSNGW 1078
            +N++ P+ LNYTSGTT++PKGVVH HR +FI+T+DSLIDWS PKQPV+LWTLPMFHSNGW
Sbjct: 181  KNDFDPIALNYTSGTTASPKGVVHCHRGLFIVTLDSLIDWSFPKQPVFLWTLPMFHSNGW 240

Query: 1077 SFTWGMAAVGGTNICLRKFDATLIHTAIPKYGVTHMCGAPVVLNMLSNSPK-AKKLKKPV 901
            S+ WGMAAVGGTNIC+RKFDA  I+  I K+ VTHMCGAPVVLNMLSNSP   K LK PV
Sbjct: 241  SYPWGMAAVGGTNICIRKFDAPTIYGLIEKHRVTHMCGAPVVLNMLSNSPTIIKPLKNPV 300

Query: 900  HFLTGGAPPPAAVLLRTEALGFIVSHGFGMTEVAGVVVSCAWKRHWNVLPALERARLKAR 721
            H +T GAPPPA +L RTE+LGF+V HG+G+TE  G+VVSCAWKR W++ PA ERARLKAR
Sbjct: 301  HIITAGAPPPATILSRTESLGFVVGHGYGLTETGGIVVSCAWKRQWSLFPATERARLKAR 360

Query: 720  QGVRTIGMTEVNIVDPKSGIIVKPDGLTQGEIVLRGGCLMLGYLKDPEGTAKCMKNDGWL 541
            QGVRTIGM EV++VDP +G  VK DG T GEIVLRGGC+MLGYLKDP  TAKCM  +GW 
Sbjct: 361  QGVRTIGMNEVDVVDPITGTSVKRDGSTLGEIVLRGGCIMLGYLKDPVATAKCMTENGWF 420

Query: 540  YTGDVGVMHPDGYLEIKDRSKDVIIXXXXXXXXXXXXXVLYLNPVVNEAAVVARPDEFWG 361
            YTGDVGVMHPDGYLEIKDRSKDVII             VLY  P +NEAAVVARPDEFWG
Sbjct: 421  YTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTFPDINEAAVVARPDEFWG 480

Query: 360  ETPCAFVSLKVAAGMIENAKEKEIMEFCRDRLPHYMVPKTVVFMGELPKNATGKIQKFSL 181
            ETPCAFV+LK A    ++  EKEI+E+CR RLPHYMVPKTVV   ELPK ATGKIQK  L
Sbjct: 481  ETPCAFVTLKEAC--CKSTTEKEIIEYCRARLPHYMVPKTVVVTEELPKTATGKIQKALL 538

Query: 180  RDIAKSLGNSPASRL 136
            RD+AK +G+S  SR+
Sbjct: 539  RDMAKDMGSSRVSRM 553