BLASTX nr result
ID: Bupleurum21_contig00018186
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00018186
(1185 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002284901.1| PREDICTED: uncharacterized protein LOC100258... 166 e-115
ref|XP_004147268.1| PREDICTED: uncharacterized protein LOC101214... 169 e-113
emb|CBI16268.3| unnamed protein product [Vitis vinifera] 166 e-110
gb|AFK76482.1| tRNA ligase [Solanum melongena] 176 e-110
ref|XP_002526346.1| hypothetical protein RCOM_1399320 [Ricinus c... 159 e-105
>ref|XP_002284901.1| PREDICTED: uncharacterized protein LOC100258617 [Vitis vinifera]
Length = 1165
Score = 166 bits (421), Expect(6) = e-115
Identities = 78/101 (77%), Positives = 86/101 (85%), Gaps = 2/101 (1%)
Frame = +1
Query: 535 IYTLL--FVLGRTFREAWGTLANKKQSEFNDYLERNRMCISMELVTTVLGDHGQRPREDY 708
IYT + FVLGR F EAWGT A KKQ EFND++ERNR+ ISMELVT VLGDHGQRP+EDY
Sbjct: 232 IYTAVGVFVLGRMFHEAWGTAARKKQVEFNDFIERNRISISMELVTAVLGDHGQRPQEDY 291
Query: 709 VVVTAVTELGKGKPVFYSTPDVIGFCRKWHLPTNHVWMFST 831
VVVTAVTELG GKP FYSTPD+I FCR+W LPTNHVW+ ST
Sbjct: 292 VVVTAVTELGNGKPKFYSTPDIIAFCREWRLPTNHVWLLST 332
Score = 161 bits (407), Expect(6) = e-115
Identities = 80/103 (77%), Positives = 90/103 (87%)
Frame = +3
Query: 243 GAKTASAEKSNTALSNMFTSNMIENFTVDKSTYSRAQIRATFYPKFENEKSDQEVRMKMI 422
GA+ A AEKS LS +F+SN + +FTVD STYS AQIRATFYPKFENEKSDQE+R +MI
Sbjct: 134 GAEMAVAEKSCAGLSKLFSSNALADFTVDNSTYSLAQIRATFYPKFENEKSDQEIRTRMI 193
Query: 423 ELVCKGLATVEVSLKHSGSLFMYAGHEGGSYAKNSYGNIYTAV 551
E+V KGLAT+EVSLKHSGSLFMYAG EGG+YAKNSYGNIYTAV
Sbjct: 194 EMVSKGLATLEVSLKHSGSLFMYAGPEGGAYAKNSYGNIYTAV 236
Score = 110 bits (276), Expect(6) = e-115
Identities = 54/75 (72%), Positives = 63/75 (84%)
Frame = +3
Query: 960 ISIPGSKDHIKVQGEILEGLVGRIVSHESSKHMEEILRNSHPFQLEGVNLDLGPSLREIC 1139
IS+PGSKDH+KVQGEILEGLV RIVSHESSKH+E++LR+ P E DLGPSLREIC
Sbjct: 365 ISVPGSKDHVKVQGEILEGLVARIVSHESSKHLEKVLRDFPPPPSEAAGSDLGPSLREIC 424
Query: 1140 AANRSDEKQEMIRLM 1184
AANRSDEKQ++ L+
Sbjct: 425 AANRSDEKQQIKALL 439
Score = 35.0 bits (79), Expect(6) = e-115
Identities = 15/17 (88%), Positives = 17/17 (100%)
Frame = +2
Query: 914 EGTASPVCQALDEVADI 964
EGTA+PVC+ALDEVADI
Sbjct: 349 EGTATPVCKALDEVADI 365
Score = 28.9 bits (63), Expect(6) = e-115
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = +1
Query: 187 PVMDTQSIWKPKSYGTM 237
PV ++IWKPKS+GT+
Sbjct: 94 PVQGQEAIWKPKSFGTV 110
Score = 24.3 bits (51), Expect(6) = e-115
Identities = 12/45 (26%), Positives = 25/45 (55%)
Frame = +2
Query: 2 NRAVHMEQRWKKKPQAIKGSLTAATEDVSNRLRDMNVAENSGQSH 136
+R++++ + + S + A E V+NR + V E+SGQ++
Sbjct: 31 SRSLNLRRSISDSVMSSNESASEAAEAVTNRFGGLAVDESSGQTY 75
>ref|XP_004147268.1| PREDICTED: uncharacterized protein LOC101214899 [Cucumis sativus]
Length = 1135
Score = 169 bits (428), Expect(6) = e-113
Identities = 80/101 (79%), Positives = 87/101 (86%), Gaps = 2/101 (1%)
Frame = +1
Query: 535 IYTLL--FVLGRTFREAWGTLANKKQSEFNDYLERNRMCISMELVTTVLGDHGQRPREDY 708
IYT + FVLGR FREAWG A KKQ+EFND+LE NRMCISMELVT VLGDHGQRPREDY
Sbjct: 207 IYTAVGVFVLGRMFREAWGAEAAKKQAEFNDFLESNRMCISMELVTAVLGDHGQRPREDY 266
Query: 709 VVVTAVTELGKGKPVFYSTPDVIGFCRKWHLPTNHVWMFST 831
VVVTAVTELGKGKP FYST ++I FCR W LPTNHVW+FS+
Sbjct: 267 VVVTAVTELGKGKPKFYSTAEIIAFCRNWRLPTNHVWLFSS 307
Score = 157 bits (396), Expect(6) = e-113
Identities = 75/98 (76%), Positives = 87/98 (88%)
Frame = +3
Query: 258 SAEKSNTALSNMFTSNMIENFTVDKSTYSRAQIRATFYPKFENEKSDQEVRMKMIELVCK 437
+A+ +LS +F SN IE FTVD STY++AQIRATFYPKFENEKSDQE+R +MIE+V K
Sbjct: 114 AAQDGVVSLSQLFKSNQIEKFTVDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIEMVSK 173
Query: 438 GLATVEVSLKHSGSLFMYAGHEGGSYAKNSYGNIYTAV 551
GLAT+EVSLKHSGSLFMYAGHEGG+YAKNS+GNIYTAV
Sbjct: 174 GLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAV 211
Score = 105 bits (263), Expect(6) = e-113
Identities = 54/77 (70%), Positives = 63/77 (81%), Gaps = 1/77 (1%)
Frame = +3
Query: 957 QISIPGSKDHIKVQGEILEGLVGRIVSHESSKHMEEILRNSHPF-QLEGVNLDLGPSLRE 1133
+IS+PGSKDHIKVQGEILEGLV R+VSHESSKHM+++L EG LDLGPSLRE
Sbjct: 339 EISVPGSKDHIKVQGEILEGLVARMVSHESSKHMQKVLEEFPALPDNEGGGLDLGPSLRE 398
Query: 1134 ICAANRSDEKQEMIRLM 1184
ICAANRSDEKQ++ L+
Sbjct: 399 ICAANRSDEKQQIKALL 415
Score = 32.3 bits (72), Expect(6) = e-113
Identities = 21/51 (41%), Positives = 29/51 (56%), Gaps = 2/51 (3%)
Frame = +2
Query: 20 EQRWKKKPQAIKGSL--TAATEDVSNRLRDMNVAENSGQSHTSVVSARHGD 166
EQ+WK+K +A + S AA E V+N L + V E S Q H SA+ G+
Sbjct: 12 EQKWKEKAKADRNSTESEAAAEVVTNALGKLRVTE-SDQPHVLTSSAQFGN 61
Score = 30.4 bits (67), Expect(6) = e-113
Identities = 13/17 (76%), Positives = 16/17 (94%)
Frame = +2
Query: 914 EGTASPVCQALDEVADI 964
EGTA+ VC+ALDEVA+I
Sbjct: 324 EGTATSVCKALDEVAEI 340
Score = 25.8 bits (55), Expect(6) = e-113
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +1
Query: 187 PVMDTQSIWKPKSYGT 234
P + ++IWKPK+YGT
Sbjct: 70 PGLAHRAIWKPKAYGT 85
>emb|CBI16268.3| unnamed protein product [Vitis vinifera]
Length = 1029
Score = 166 bits (421), Expect(4) = e-110
Identities = 78/101 (77%), Positives = 86/101 (85%), Gaps = 2/101 (1%)
Frame = +1
Query: 535 IYTLL--FVLGRTFREAWGTLANKKQSEFNDYLERNRMCISMELVTTVLGDHGQRPREDY 708
IYT + FVLGR F EAWGT A KKQ EFND++ERNR+ ISMELVT VLGDHGQRP+EDY
Sbjct: 96 IYTAVGVFVLGRMFHEAWGTAARKKQVEFNDFIERNRISISMELVTAVLGDHGQRPQEDY 155
Query: 709 VVVTAVTELGKGKPVFYSTPDVIGFCRKWHLPTNHVWMFST 831
VVVTAVTELG GKP FYSTPD+I FCR+W LPTNHVW+ ST
Sbjct: 156 VVVTAVTELGNGKPKFYSTPDIIAFCREWRLPTNHVWLLST 196
Score = 157 bits (397), Expect(4) = e-110
Identities = 78/99 (78%), Positives = 87/99 (87%)
Frame = +3
Query: 255 ASAEKSNTALSNMFTSNMIENFTVDKSTYSRAQIRATFYPKFENEKSDQEVRMKMIELVC 434
A AEKS LS +F+SN + +FTVD STYS AQIRATFYPKFENEKSDQE+R +MIE+V
Sbjct: 2 AVAEKSCAGLSKLFSSNALADFTVDNSTYSLAQIRATFYPKFENEKSDQEIRTRMIEMVS 61
Query: 435 KGLATVEVSLKHSGSLFMYAGHEGGSYAKNSYGNIYTAV 551
KGLAT+EVSLKHSGSLFMYAG EGG+YAKNSYGNIYTAV
Sbjct: 62 KGLATLEVSLKHSGSLFMYAGPEGGAYAKNSYGNIYTAV 100
Score = 110 bits (276), Expect(4) = e-110
Identities = 54/75 (72%), Positives = 63/75 (84%)
Frame = +3
Query: 960 ISIPGSKDHIKVQGEILEGLVGRIVSHESSKHMEEILRNSHPFQLEGVNLDLGPSLREIC 1139
IS+PGSKDH+KVQGEILEGLV RIVSHESSKH+E++LR+ P E DLGPSLREIC
Sbjct: 229 ISVPGSKDHVKVQGEILEGLVARIVSHESSKHLEKVLRDFPPPPSEAAGSDLGPSLREIC 288
Query: 1140 AANRSDEKQEMIRLM 1184
AANRSDEKQ++ L+
Sbjct: 289 AANRSDEKQQIKALL 303
Score = 35.0 bits (79), Expect(4) = e-110
Identities = 15/17 (88%), Positives = 17/17 (100%)
Frame = +2
Query: 914 EGTASPVCQALDEVADI 964
EGTA+PVC+ALDEVADI
Sbjct: 213 EGTATPVCKALDEVADI 229
>gb|AFK76482.1| tRNA ligase [Solanum melongena]
Length = 1167
Score = 176 bits (447), Expect(4) = e-110
Identities = 82/101 (81%), Positives = 90/101 (89%), Gaps = 2/101 (1%)
Frame = +1
Query: 535 IYTLL--FVLGRTFREAWGTLANKKQSEFNDYLERNRMCISMELVTTVLGDHGQRPREDY 708
IYT + FVLGR FREAWGT A+KKQ+EFN++LERNRMCISMELVT VLGDHGQRPR+DY
Sbjct: 243 IYTAVGVFVLGRMFREAWGTKASKKQAEFNEFLERNRMCISMELVTAVLGDHGQRPRDDY 302
Query: 709 VVVTAVTELGKGKPVFYSTPDVIGFCRKWHLPTNHVWMFST 831
VVTAVTELG GKP FYSTPDVI FCR+W LPTNHVW+FST
Sbjct: 303 AVVTAVTELGNGKPTFYSTPDVIAFCREWRLPTNHVWLFST 343
Score = 160 bits (404), Expect(4) = e-110
Identities = 79/121 (65%), Positives = 95/121 (78%), Gaps = 10/121 (8%)
Frame = +3
Query: 219 KIVWN-----NVTGAKTASA-----EKSNTALSNMFTSNMIENFTVDKSTYSRAQIRATF 368
K++W V+GA A E+ + LS +F N++ENFTVD ST+SRAQ+RATF
Sbjct: 127 KVIWKPKSYGTVSGAPVVEAGKTPVEQKSALLSKLFKGNLLENFTVDNSTFSRAQVRATF 186
Query: 369 YPKFENEKSDQEVRMKMIELVCKGLATVEVSLKHSGSLFMYAGHEGGSYAKNSYGNIYTA 548
YPKFENEKSDQE+R +MIE+V KGLA VEV+LKHSGSLFMYAGHEGG+YAKNS+GNIYTA
Sbjct: 187 YPKFENEKSDQEIRTRMIEMVSKGLAIVEVTLKHSGSLFMYAGHEGGAYAKNSFGNIYTA 246
Query: 549 V 551
V
Sbjct: 247 V 247
Score = 102 bits (253), Expect(4) = e-110
Identities = 50/69 (72%), Positives = 58/69 (84%)
Frame = +3
Query: 960 ISIPGSKDHIKVQGEILEGLVGRIVSHESSKHMEEILRNSHPFQLEGVNLDLGPSLREIC 1139
IS+PGSKDHIKVQGEILEGLV RIV ESS+HME +LR+ P EG LDLGP+LREIC
Sbjct: 376 ISVPGSKDHIKVQGEILEGLVARIVKRESSEHMERVLRDFPPPPSEGEGLDLGPTLREIC 435
Query: 1140 AANRSDEKQ 1166
AANRS+++Q
Sbjct: 436 AANRSEKQQ 444
Score = 30.0 bits (66), Expect(4) = e-110
Identities = 13/17 (76%), Positives = 15/17 (88%)
Frame = +2
Query: 914 EGTASPVCQALDEVADI 964
EGTA+ VC+AL EVADI
Sbjct: 360 EGTATTVCEALSEVADI 376
>ref|XP_002526346.1| hypothetical protein RCOM_1399320 [Ricinus communis]
gi|223534305|gb|EEF36017.1| hypothetical protein
RCOM_1399320 [Ricinus communis]
Length = 747
Score = 159 bits (401), Expect(4) = e-105
Identities = 79/118 (66%), Positives = 95/118 (80%), Gaps = 7/118 (5%)
Frame = +3
Query: 219 KIVWN-----NVTGAKTAS--AEKSNTALSNMFTSNMIENFTVDKSTYSRAQIRATFYPK 377
K++W V+GA KSN LS +F N++ENFTVD STY++AQIRATFYPK
Sbjct: 51 KLIWKPRSYGTVSGAVEVENVVPKSNVNLSQLFKGNLLENFTVDNSTYAQAQIRATFYPK 110
Query: 378 FENEKSDQEVRMKMIELVCKGLATVEVSLKHSGSLFMYAGHEGGSYAKNSYGNIYTAV 551
FENEKSDQE+R++MIE+V K LATVEV+LKHSGSLFMYAGH+GG+YAKNS+GNIYTAV
Sbjct: 111 FENEKSDQEIRIRMIEMVSKDLATVEVTLKHSGSLFMYAGHKGGAYAKNSFGNIYTAV 168
Score = 153 bits (387), Expect(4) = e-105
Identities = 75/101 (74%), Positives = 83/101 (82%), Gaps = 2/101 (1%)
Frame = +1
Query: 535 IYTLL--FVLGRTFREAWGTLANKKQSEFNDYLERNRMCISMELVTTVLGDHGQRPREDY 708
IYT + FVLGR FREAWG A +KQ+EFN++LERNRMCISMELVT VLGDHGQ
Sbjct: 164 IYTAVGVFVLGRMFREAWGAAAARKQAEFNEFLERNRMCISMELVTAVLGDHGQ------ 217
Query: 709 VVVTAVTELGKGKPVFYSTPDVIGFCRKWHLPTNHVWMFST 831
+VVTAVTELG GKP FYSTP+VI FCRKW LPTNHVW+FST
Sbjct: 218 LVVTAVTELGNGKPKFYSTPEVIAFCRKWRLPTNHVWLFST 258
Score = 112 bits (280), Expect(4) = e-105
Identities = 55/69 (79%), Positives = 59/69 (85%)
Frame = +3
Query: 960 ISIPGSKDHIKVQGEILEGLVGRIVSHESSKHMEEILRNSHPFQLEGVNLDLGPSLREIC 1139
IS+PGSKDHIKVQGEILEGLV RIVSH+SSKHM E+LR P EG LDLGPSLREIC
Sbjct: 291 ISVPGSKDHIKVQGEILEGLVARIVSHDSSKHMNEVLREYPPPPAEGAELDLGPSLREIC 350
Query: 1140 AANRSDEKQ 1166
AANR+DEKQ
Sbjct: 351 AANRTDEKQ 359
Score = 28.1 bits (61), Expect(4) = e-105
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = +2
Query: 914 EGTASPVCQALDEVADI 964
EGTA+ VC ALDEVA I
Sbjct: 275 EGTATTVCSALDEVAVI 291