BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00018157
         (1850 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAL30454.1|AF362949_1 endo-beta-1,4-glucanase precursor [Nico...   748   0.0  
ref|XP_002299478.1| predicted protein [Populus trichocarpa] gi|2...   736   0.0  
ref|XP_003530388.1| PREDICTED: endoglucanase 6-like [Glycine max]     733   0.0  
ref|XP_002270880.1| PREDICTED: endoglucanase 6 [Vitis vinifera] ...   733   0.0  
emb|CAI68019.1| endo-beta-1,4-glucanase [Prunus persica]              731   0.0  

>gb|AAL30454.1|AF362949_1 endo-beta-1,4-glucanase precursor [Nicotiana tabacum]
          Length = 624

 Score =  748 bits (1932), Expect = 0.0
 Identities = 365/501 (72%), Positives = 394/501 (78%)
 Frame = -1

Query: 1850 YLIKAHPEPNVFYGEVGDGNTDHYCWQRPEDMTTSRQAYRIDPSNPGSDLAGEXXXXXXX 1671
            YL+KAHPEPNV YGEVGDG TDHYCWQRPEDMTTSR AYRIDPS PGSDLAGE       
Sbjct: 130  YLLKAHPEPNVLYGEVGDGTTDHYCWQRPEDMTTSRAAYRIDPSRPGSDLAGETAAAMAA 189

Query: 1670 XXMVFRRSDPSYSKTLLSHAYQLFDFADKYRGKYDGSITVAQKYYRSVSGYADELLWGAA 1491
              +VFR S+P+Y+K LL+HAYQLF+FADKYRGKYD SITVAQKYYRSVSGYADELLW AA
Sbjct: 190  ASIVFRNSNPAYAKELLTHAYQLFEFADKYRGKYDSSITVAQKYYRSVSGYADELLWAAA 249

Query: 1490 WLYKATNSEYYLNYLGNNGDALGGTGWAMTEFGWDVKYAGVQTLVAKFLMGGNTGNHASV 1311
            WLYKA+N EYYLNYLG NGDALGGTGW+MTEFGWDVKYAGVQTL AKFLM GN GNHA V
Sbjct: 250  WLYKASNKEYYLNYLGENGDALGGTGWSMTEFGWDVKYAGVQTLAAKFLMQGNAGNHAPV 309

Query: 1310 FEQYQEKAEFFMCSCLGKSNHNVQKTPGGLIFRQKWNNLQFVTSASFLLTVYSDYLTSAG 1131
            FE+YQEKAE FMC+CLGK N N+ K+PGGLIFRQ+WNN+QFVTSASFL TVYSDYL SA 
Sbjct: 310  FEKYQEKAENFMCACLGKGNQNIHKSPGGLIFRQRWNNMQFVTSASFLATVYSDYLASAR 369

Query: 1130 KTLKCSSGNVQPSELFSFAKSQVDYILGDNPRATSYMVGYGSNYPRQTHHRGSSIVSIKD 951
            K+LKCSSG V PSEL SFAKSQVDYILGDNPRATSYMVGYG+NYPRQ HHRGSSIVS+K 
Sbjct: 370  KSLKCSSGTVLPSELLSFAKSQVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSVKK 429

Query: 950  DPSFVSCRGGYATWFSRKSSDPNLLNGAIVGGPDAYDNFADQRDNYEQTEPATYNNAPXX 771
            DPSFVSCRGGYATWFSRK+SDPNLL GAIVGGPDAYDNFADQRDNYEQTEPATYNNAP  
Sbjct: 430  DPSFVSCRGGYATWFSRKASDPNLLAGAIVGGPDAYDNFADQRDNYEQTEPATYNNAPLI 489

Query: 770  XXXXXXXXXXXGYNQLLPVILPTPKAIAVQXXXXXXXXXXXXPVASMQQIAVEQKITTSW 591
                        Y+QLLPV +P PK    Q            P +S   I +EQK T SW
Sbjct: 490  GVLARLHGGQSKYSQLLPVAIPQPKPDPEQ-------KVTPAPASSTADITIEQKETASW 542

Query: 590  VSGGKTYYRYXXXXXXXXXXXXXXXXXXXXXLYGPLWGLEKSGNVYVFPAWIKQLDAGKS 411
            V  GKTYYRY                     LYG LWGL K G+ YVFPAW+  L AGK+
Sbjct: 543  VPKGKTYYRYSVIVTNKSAMTMKNLKLSIYQLYGSLWGLSKYGDSYVFPAWLNSLPAGKT 602

Query: 410  IEFVYIHSASPAAVSVSRYTL 348
            +EFVY+HSASPA VS+S YTL
Sbjct: 603  LEFVYVHSASPATVSISSYTL 623


>ref|XP_002299478.1| predicted protein [Populus trichocarpa] gi|222846736|gb|EEE84283.1|
            predicted protein [Populus trichocarpa]
            gi|347466587|gb|AEO97206.1| endo-1,4-beta-glucanase
            [Populus trichocarpa] gi|347466641|gb|AEO97233.1|
            endo-1,4-beta-glucanase [Populus trichocarpa]
          Length = 622

 Score =  736 bits (1899), Expect = 0.0
 Identities = 351/501 (70%), Positives = 398/501 (79%)
 Frame = -1

Query: 1850 YLIKAHPEPNVFYGEVGDGNTDHYCWQRPEDMTTSRQAYRIDPSNPGSDLAGEXXXXXXX 1671
            YLIKAHP+P+V YGEVGDGNTDH+CWQRPEDMTT R+AY+IDPSNPGSDLAGE       
Sbjct: 125  YLIKAHPQPDVLYGEVGDGNTDHHCWQRPEDMTTDRRAYKIDPSNPGSDLAGETAAAMAA 184

Query: 1670 XXMVFRRSDPSYSKTLLSHAYQLFDFADKYRGKYDGSITVAQKYYRSVSGYADELLWGAA 1491
              +VFRRS+PSY+  LL+HA QLFDFAD+YRGKYD SITVAQKYYRSVSGY DELLW AA
Sbjct: 185  ASIVFRRSNPSYANELLTHARQLFDFADRYRGKYDSSITVAQKYYRSVSGYNDELLWAAA 244

Query: 1490 WLYKATNSEYYLNYLGNNGDALGGTGWAMTEFGWDVKYAGVQTLVAKFLMGGNTGNHASV 1311
            W+Y+ATN++YYLNYLGNNGD++GGTGW MTEFGWDVKYAGVQTLVAKFLM G  G+HA V
Sbjct: 245  WMYQATNNQYYLNYLGNNGDSMGGTGWGMTEFGWDVKYAGVQTLVAKFLMQGKAGHHAPV 304

Query: 1310 FEQYQEKAEFFMCSCLGKSNHNVQKTPGGLIFRQKWNNLQFVTSASFLLTVYSDYLTSAG 1131
            FE+YQ+KAE+FMCSCLGK + NVQKTPGGLIFRQ+WNN+QFVTSASFL TVYSDYL SAG
Sbjct: 305  FEKYQQKAEYFMCSCLGKGSRNVQKTPGGLIFRQRWNNMQFVTSASFLTTVYSDYLASAG 364

Query: 1130 KTLKCSSGNVQPSELFSFAKSQVDYILGDNPRATSYMVGYGSNYPRQTHHRGSSIVSIKD 951
            + L C++GNV P++L +FAKSQVDYILGDNPRATSYMVGYG+NYPRQ HHRGSSIVS K 
Sbjct: 365  RNLNCAAGNVAPTQLLAFAKSQVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSYKV 424

Query: 950  DPSFVSCRGGYATWFSRKSSDPNLLNGAIVGGPDAYDNFADQRDNYEQTEPATYNNAPXX 771
            DP+FV+CRGGYATWFSRK SDPNLL GAIVGGPDAYDNFAD+RDNYEQTEPATYNNAP  
Sbjct: 425  DPTFVTCRGGYATWFSRKGSDPNLLTGAIVGGPDAYDNFADERDNYEQTEPATYNNAPLL 484

Query: 770  XXXXXXXXXXXGYNQLLPVILPTPKAIAVQXXXXXXXXXXXXPVASMQQIAVEQKITTSW 591
                       GYNQLLPV++P P    ++            P ++   +A+ QK T +W
Sbjct: 485  GLLARLSGGHGGYNQLLPVVVPAP----IEKKPAPQTKITPAPASTSAPVAIVQKTTATW 540

Query: 590  VSGGKTYYRYXXXXXXXXXXXXXXXXXXXXXLYGPLWGLEKSGNVYVFPAWIKQLDAGKS 411
            ++ GKTYYRY                     LYGPLWGL KSGN Y FP+WI  L AGKS
Sbjct: 541  IAKGKTYYRYSTIVTNKSAKELKDIKLSISKLYGPLWGLTKSGNFYAFPSWITSLAAGKS 600

Query: 410  IEFVYIHSASPAAVSVSRYTL 348
            +EFVY+HSAS A VSVS YTL
Sbjct: 601  LEFVYVHSASAADVSVSSYTL 621


>ref|XP_003530388.1| PREDICTED: endoglucanase 6-like [Glycine max]
          Length = 625

 Score =  733 bits (1893), Expect = 0.0
 Identities = 349/501 (69%), Positives = 397/501 (79%)
 Frame = -1

Query: 1850 YLIKAHPEPNVFYGEVGDGNTDHYCWQRPEDMTTSRQAYRIDPSNPGSDLAGEXXXXXXX 1671
            Y IKAHP+ NV YGEVGDGNTDHYCWQRPEDMTT R AY++DPSNPGSDLAGE       
Sbjct: 124  YFIKAHPQANVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKVDPSNPGSDLAGETAAAMAA 183

Query: 1670 XXMVFRRSDPSYSKTLLSHAYQLFDFADKYRGKYDGSITVAQKYYRSVSGYADELLWGAA 1491
              +VFRRS+P+Y+  LL HAYQLFDFADKYRGKYD SITVAQKYYRS+SGY DELLW AA
Sbjct: 184  ASIVFRRSNPAYAGELLRHAYQLFDFADKYRGKYDSSITVAQKYYRSISGYNDELLWAAA 243

Query: 1490 WLYKATNSEYYLNYLGNNGDALGGTGWAMTEFGWDVKYAGVQTLVAKFLMGGNTGNHASV 1311
            WLY+A+N++YYL+YLG NGD++GGTGW+MTEFGWDVKYAGVQTLVAKFLM G +G+HA V
Sbjct: 244  WLYQASNNQYYLDYLGRNGDSMGGTGWSMTEFGWDVKYAGVQTLVAKFLMQGKSGHHAPV 303

Query: 1310 FEQYQEKAEFFMCSCLGKSNHNVQKTPGGLIFRQKWNNLQFVTSASFLLTVYSDYLTSAG 1131
            FE+YQ+KAE FMCSCLGKSN NVQKTPGGLIFRQ+WNN+QFVTSASFL TVYSDYL S+G
Sbjct: 304  FERYQQKAETFMCSCLGKSNRNVQKTPGGLIFRQRWNNMQFVTSASFLATVYSDYLASSG 363

Query: 1130 KTLKCSSGNVQPSELFSFAKSQVDYILGDNPRATSYMVGYGSNYPRQTHHRGSSIVSIKD 951
            + L+CSSGNV P+EL S AKSQVDY+LGDNPRATSYMVGYGSN+P++ HHRGSSIVSIK 
Sbjct: 364  RNLRCSSGNVPPAELLSLAKSQVDYLLGDNPRATSYMVGYGSNFPQRVHHRGSSIVSIKV 423

Query: 950  DPSFVSCRGGYATWFSRKSSDPNLLNGAIVGGPDAYDNFADQRDNYEQTEPATYNNAPXX 771
            +PSFVSCRGGYATWFS K SDPNLL GA+VGGPDAYD+FAD+RDNYEQTEPATYNNAP  
Sbjct: 424  NPSFVSCRGGYATWFSSKRSDPNLLTGALVGGPDAYDDFADERDNYEQTEPATYNNAPLI 483

Query: 770  XXXXXXXXXXXGYNQLLPVILPTPKAIAVQXXXXXXXXXXXXPVASMQQIAVEQKITTSW 591
                       GYNQLLPV++P PK    +            P +    I++EQK TTSW
Sbjct: 484  GILARLGGGHGGYNQLLPVVVPAPKPAVTKPQPTPSPKTTPSPASWSGPISIEQKRTTSW 543

Query: 590  VSGGKTYYRYXXXXXXXXXXXXXXXXXXXXXLYGPLWGLEKSGNVYVFPAWIKQLDAGKS 411
            V+ GKTYYRY                     LYGP+WG+ KSG+ Y FP+W+  L AGKS
Sbjct: 544  VANGKTYYRYSTVVTNKSNKSLNSLNLSISKLYGPIWGVTKSGDSYTFPSWLSSLSAGKS 603

Query: 410  IEFVYIHSASPAAVSVSRYTL 348
            +EFVYIHSASPA VSV+ Y L
Sbjct: 604  LEFVYIHSASPADVSVANYVL 624


>ref|XP_002270880.1| PREDICTED: endoglucanase 6 [Vitis vinifera]
            gi|147821654|emb|CAN66000.1| hypothetical protein
            VITISV_031862 [Vitis vinifera]
            gi|297742327|emb|CBI34476.3| unnamed protein product
            [Vitis vinifera]
          Length = 629

 Score =  733 bits (1891), Expect = 0.0
 Identities = 351/501 (70%), Positives = 394/501 (78%)
 Frame = -1

Query: 1850 YLIKAHPEPNVFYGEVGDGNTDHYCWQRPEDMTTSRQAYRIDPSNPGSDLAGEXXXXXXX 1671
            YLIKAHP P+V YGEVGDGNTDH+CWQRPEDMTT R+AY+IDPSNPGSDLAGE       
Sbjct: 128  YLIKAHPHPDVLYGEVGDGNTDHFCWQRPEDMTTDRRAYKIDPSNPGSDLAGETAAAMAA 187

Query: 1670 XXMVFRRSDPSYSKTLLSHAYQLFDFADKYRGKYDGSITVAQKYYRSVSGYADELLWGAA 1491
              +VFRRS+PSY+  LL+HA QLF+F DKYRGKYD SITVAQKYYRS SGYADELLW AA
Sbjct: 188  ASIVFRRSNPSYANELLTHARQLFEFGDKYRGKYDSSITVAQKYYRSYSGYADELLWAAA 247

Query: 1490 WLYKATNSEYYLNYLGNNGDALGGTGWAMTEFGWDVKYAGVQTLVAKFLMGGNTGNHASV 1311
            WLY+ATN++YYL+YLGNNG +LGGTGW+MTEFGWD KYAGVQ LVAK LM G  G + SV
Sbjct: 248  WLYQATNNQYYLSYLGNNGQSLGGTGWSMTEFGWDAKYAGVQVLVAKMLMQGKGGRYTSV 307

Query: 1310 FEQYQEKAEFFMCSCLGKSNHNVQKTPGGLIFRQKWNNLQFVTSASFLLTVYSDYLTSAG 1131
            F+QYQ+KAEFFMCSCLGK   N+QKTPGGL++RQ+WNN+QFVTSASFL+TVYSDYL SAG
Sbjct: 308  FQQYQQKAEFFMCSCLGKGTRNIQKTPGGLLYRQRWNNMQFVTSASFLVTVYSDYLASAG 367

Query: 1130 KTLKCSSGNVQPSELFSFAKSQVDYILGDNPRATSYMVGYGSNYPRQTHHRGSSIVSIKD 951
            K L C++G+V PS L SFAKSQVDYILGDNPRATSYMVGYG+NYPRQ HHRGSSIVSIK 
Sbjct: 368  KNLNCAAGSVSPSGLISFAKSQVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKV 427

Query: 950  DPSFVSCRGGYATWFSRKSSDPNLLNGAIVGGPDAYDNFADQRDNYEQTEPATYNNAPXX 771
            +PSFVSCRGGYATWFSRK SDPNLL GAIVGGPDAYDNFAD+RDNYEQTEPATYNNAP  
Sbjct: 428  NPSFVSCRGGYATWFSRKGSDPNLLTGAIVGGPDAYDNFADERDNYEQTEPATYNNAPLI 487

Query: 770  XXXXXXXXXXXGYNQLLPVILPTPKAIAVQXXXXXXXXXXXXPVASMQQIAVEQKITTSW 591
                       GYNQLLPV+LP PK +  Q            P ++  +IA+EQK+TTSW
Sbjct: 488  GLLARLSGGHGGYNQLLPVVLPAPKEVVTQPKSAPTPKVTPAPASTSAEIAIEQKLTTSW 547

Query: 590  VSGGKTYYRYXXXXXXXXXXXXXXXXXXXXXLYGPLWGLEKSGNVYVFPAWIKQLDAGKS 411
             + G TY RY                     LYGPLWGL KSG+ Y FP+W+K L AG+ 
Sbjct: 548  KAKGNTYNRYSTIVTNKSAKTLKDLELTISKLYGPLWGLTKSGDSYGFPSWLKALAAGEK 607

Query: 410  IEFVYIHSASPAAVSVSRYTL 348
            +EFVYIHSA PA VSVS YTL
Sbjct: 608  LEFVYIHSADPAEVSVSSYTL 628


>emb|CAI68019.1| endo-beta-1,4-glucanase [Prunus persica]
          Length = 620

 Score =  731 bits (1887), Expect = 0.0
 Identities = 352/501 (70%), Positives = 394/501 (78%)
 Frame = -1

Query: 1850 YLIKAHPEPNVFYGEVGDGNTDHYCWQRPEDMTTSRQAYRIDPSNPGSDLAGEXXXXXXX 1671
            Y IKAHPEP V YGEVGDGNTDHYCWQRPEDMTT R+AY+I PSNPGSDLAGE       
Sbjct: 125  YFIKAHPEPYVLYGEVGDGNTDHYCWQRPEDMTTDRRAYKISPSNPGSDLAGETAAAMAA 184

Query: 1670 XXMVFRRSDPSYSKTLLSHAYQLFDFADKYRGKYDGSITVAQKYYRSVSGYADELLWGAA 1491
              +VFR S+P+Y+  LL HA+QLFDFADKYRGKYD SITVAQKYYRS+SGY DELLW AA
Sbjct: 185  ASIVFRHSNPAYASELLRHAHQLFDFADKYRGKYDSSITVAQKYYRSISGYNDELLWAAA 244

Query: 1490 WLYKATNSEYYLNYLGNNGDALGGTGWAMTEFGWDVKYAGVQTLVAKFLMGGNTGNHASV 1311
            WL++ATN++YYLNYLGNNGD++GGTGW MTEFGWDVKY GVQTLVAKFLM G  G + +V
Sbjct: 245  WLFQATNNQYYLNYLGNNGDSMGGTGWGMTEFGWDVKYPGVQTLVAKFLMQGKAGRNTAV 304

Query: 1310 FEQYQEKAEFFMCSCLGKSNHNVQKTPGGLIFRQKWNNLQFVTSASFLLTVYSDYLTSAG 1131
            FE+YQ+KAE+FMCSC+GK + N QKTPGGLIFRQ+WNN+QFVTSASFL TVYSDYLTSAG
Sbjct: 305  FEKYQQKAEYFMCSCIGKGSRNAQKTPGGLIFRQRWNNMQFVTSASFLATVYSDYLTSAG 364

Query: 1130 KTLKCSSGNVQPSELFSFAKSQVDYILGDNPRATSYMVGYGSNYPRQTHHRGSSIVSIKD 951
            KTL C++GNV PSEL SFAKSQVDYILGDNPRATSYMVGYG+N+P+Q HHR SSIVS K 
Sbjct: 365  KTLSCAAGNVAPSELLSFAKSQVDYILGDNPRATSYMVGYGNNFPQQVHHRASSIVSYKK 424

Query: 950  DPSFVSCRGGYATWFSRKSSDPNLLNGAIVGGPDAYDNFADQRDNYEQTEPATYNNAPXX 771
            D SFVSCRGGYATWFSRK+SDPNLL GAIVGGPDAYDNFADQRDNYEQTEPATYNNAP  
Sbjct: 425  DSSFVSCRGGYATWFSRKASDPNLLTGAIVGGPDAYDNFADQRDNYEQTEPATYNNAPLL 484

Query: 770  XXXXXXXXXXXGYNQLLPVILPTPKAIAVQXXXXXXXXXXXXPVASMQQIAVEQKITTSW 591
                       GYNQLLPV+   PK   +             P +S   IA++QK+TTSW
Sbjct: 485  GILARLNAGHGGYNQLLPVVTTQPKVAPL------PKITPVAPASSSSPIAIQQKLTTSW 538

Query: 590  VSGGKTYYRYXXXXXXXXXXXXXXXXXXXXXLYGPLWGLEKSGNVYVFPAWIKQLDAGKS 411
            VS G TYYRY                     LYGP+WGL K+G+ YVFP+WI  L AGKS
Sbjct: 539  VSKGVTYYRYSTVVTNKSAKTLTNLKLSVSKLYGPIWGLTKAGDSYVFPSWINSLSAGKS 598

Query: 410  IEFVYIHSASPAAVSVSRYTL 348
            +EFVYIHSASPA V+VS Y+L
Sbjct: 599  LEFVYIHSASPAVVAVSSYSL 619