BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00018146
         (1255 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002264927.2| PREDICTED: E3 ubiquitin-protein ligase PUB23...   508   e-141
ref|XP_002264761.1| PREDICTED: E3 ubiquitin-protein ligase PUB23...   499   e-139
ref|XP_004134357.1| PREDICTED: E3 ubiquitin-protein ligase PUB23...   406   e-111
ref|XP_004155855.1| PREDICTED: E3 ubiquitin-protein ligase PUB23...   327   3e-87
ref|XP_002447013.1| hypothetical protein SORBIDRAFT_06g026870 [S...   327   4e-87

>ref|XP_002264927.2| PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Vitis vinifera]
          Length = 413

 Score =  508 bits (1308), Expect = e-141
 Identities = 257/408 (62%), Positives = 324/408 (79%), Gaps = 3/408 (0%)
 Frame = +2

Query: 8    MDFPLDFRCPISMELMKDPVTISTGVSYERKHIEKWINSYQKKTCPATMQTLDNFEVTPN 187
            MDFP+DFRCPISMELM+DPVTISTGV+YERK+IEKW+ SY KKTCP TMQ +++F++TPN
Sbjct: 3    MDFPVDFRCPISMELMQDPVTISTGVTYERKNIEKWLFSYNKKTCPTTMQRINSFDITPN 62

Query: 188  HTLKRLILSWRNSYCXXXXXXXXXXXIKHEEFVSLLNTIDSSCPFKVSSLKKLRSIVQMS 367
            HTLKRLIL+W+N              +KH+E VSLL T++S+ PFKV+SLKKLR+IV + 
Sbjct: 63   HTLKRLILAWQNEEAARSCESRPVRSLKHDELVSLLATMEST-PFKVNSLKKLRAIVGLG 121

Query: 368  EEIKIDFKRCGGXXXXXXXXXXXXXXNSDFSIFRACEEALGVLHE-LPLSDDDDETILLL 544
             E+K DF RCGG                DF  F ACEEALGVLH+ L LS++D++TI LL
Sbjct: 122  HEVKDDFMRCGGVEVLVQIIAQILIETFDFVAFTACEEALGVLHQYLSLSEEDEKTIQLL 181

Query: 545  SKPECIKSMTIMLQRGSAQSRFYTISIIHKMTRVDYDWK-YIAEDQGIDFFKALLELVSD 721
            SKPE IKS+ IMLQRGS ++RFY ++I  K+ +VDY+WK ++ +DQG +FF++LLELVSD
Sbjct: 182  SKPESIKSLAIMLQRGSGEARFYAVTIFQKLAQVDYNWKKFVIQDQGFEFFRSLLELVSD 241

Query: 722  E-MGTKSSSYALQVLIDILTSSKKSRLKAIEAGAMCILIELLPDSNRSKCEKVLQLIKLL 898
            + + TK+S+ AL++LI+IL SSK SRLK IEAGA+CILIELLPDSNRSKCEK+L L+KLL
Sbjct: 242  DQICTKASASALELLIEILGSSKTSRLKVIEAGAVCILIELLPDSNRSKCEKMLLLLKLL 301

Query: 899  CKSADGRLAFIEHGVGIGAISKKMLHVSLVSTKIVVKILWLICSFNPTNVRVIEDMLFFG 1078
            C+ A+GRLA +EHG+GI AISKK+LHVS  +TKI VKILWLICS++P+  RV+E++L FG
Sbjct: 302  CECAEGRLALVEHGLGIAAISKKLLHVSSAATKITVKILWLICSYHPSE-RVLEELLIFG 360

Query: 1079 SVLKLEALLHIDGGSSTKDIVVKILKLHGSSWMRYPCFPCDLKEFLSL 1222
            SV KL ALLHID  SSTK+ VVKI KLHG+SW + PCFPC+LK+ L L
Sbjct: 361  SVKKLLALLHIDNRSSTKEKVVKIFKLHGNSWRQNPCFPCELKDHLGL 408


>ref|XP_002264761.1| PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Vitis vinifera]
          Length = 411

 Score =  499 bits (1284), Expect = e-139
 Identities = 254/409 (62%), Positives = 322/409 (78%), Gaps = 3/409 (0%)
 Frame = +2

Query: 8    MDFPLDFRCPISMELMKDPVTISTGVSYERKHIEKWINSYQKKTCPATMQTLDNFEVTPN 187
            MDFP+DFRCPISMELM+DPVTISTGV+YERK+IEKW+ SY  KTCP TMQ +++F++TPN
Sbjct: 1    MDFPVDFRCPISMELMQDPVTISTGVTYERKNIEKWLFSYNNKTCPTTMQRINSFDITPN 60

Query: 188  HTLKRLILSWRNSYCXXXXXXXXXXXIKHEEFVSLLNTIDSSCPFKVSSLKKLRSIVQMS 367
            HTLKRLIL+W+N              +KH+E VSLL T++S+ PFKV+SLKKLR+IV + 
Sbjct: 61   HTLKRLILAWQNEEAARSCDSRPVRSLKHDELVSLLATMEST-PFKVNSLKKLRAIVGLG 119

Query: 368  EEIKIDFKRCGGXXXXXXXXXXXXXXNSDFSIFRACEEALGVLHE-LPLSDDDDETILLL 544
             E+K DF RCGG              +SDF  F ACEEALGVLH+ L LS+DD++TI LL
Sbjct: 120  HEVKDDFMRCGGVEVLVQIIAQILVESSDFVAFTACEEALGVLHQYLYLSEDDEKTIQLL 179

Query: 545  SKPECIKSMTIMLQRGSAQSRFYTISIIHKMTRVDYDWK-YIAEDQGIDFFKALLELVSD 721
            SKPE IKS+ IMLQRGS ++RF  ++I  K+ + DY+WK ++ +DQG +FF++LLELVSD
Sbjct: 180  SKPESIKSLAIMLQRGSGEARFCAVTIFQKLAQADYNWKKFVIQDQGFEFFRSLLELVSD 239

Query: 722  E-MGTKSSSYALQVLIDILTSSKKSRLKAIEAGAMCILIELLPDSNRSKCEKVLQLIKLL 898
            + + TK+S+ AL++LI+IL SSKKSRLKAIEAGA+CILIELLPDSNRSKCEK+L L+KLL
Sbjct: 240  DQICTKASASALELLIEILGSSKKSRLKAIEAGAVCILIELLPDSNRSKCEKMLLLLKLL 299

Query: 899  CKSADGRLAFIEHGVGIGAISKKMLHVSLVSTKIVVKILWLICSFNPTNVRVIEDMLFFG 1078
            C+ A+GRLA +EHG+GI AISKK+LHVS  STKI VKILWLICS++P+  RV+E+ML  G
Sbjct: 300  CECAEGRLALVEHGLGIAAISKKLLHVSSASTKITVKILWLICSYHPSE-RVLEEMLILG 358

Query: 1079 SVLKLEALLHIDGGSSTKDIVVKILKLHGSSWMRYPCFPCDLKEFLSLS 1225
            SV KL  LLHI   SSTK+ VVKI +LH +SW +YPCFP +LK++  L+
Sbjct: 359  SVKKLLVLLHIGNRSSTKEKVVKIFRLHRNSWRQYPCFPWELKDYSGLT 407


>ref|XP_004134357.1| PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis sativus]
          Length = 415

 Score =  406 bits (1044), Expect = e-111
 Identities = 220/417 (52%), Positives = 298/417 (71%), Gaps = 12/417 (2%)
 Frame = +2

Query: 2    LNMDFPLDFRCPISMELMKDPVTISTGVSYERKHIEKWINSYQKKTCPATMQTL----DN 169
            ++ DFP  FRCPISMELM+DPVT+STGV+Y+RK IEKW+ +Y KKTCPATMQTL    ++
Sbjct: 1    MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60

Query: 170  FEVTPNHTLKRLILSWRNSYCXXXXXXXXXXXIKHEEFVSLLNTIDSSCPFKVSSLKKLR 349
            F +TPN  LKRLIL+W+ +                EE  SLL T++SS PFKVSSL+KLR
Sbjct: 61   FVITPNLNLKRLILAWKINDSALTPATAL------EEVSSLLATVESS-PFKVSSLRKLR 113

Query: 350  SIVQMSEEIKIDFKRCGGXXXXXXXXXXXXXXNSDFSIFRACEEALGVLHELPLSDDDDE 529
            S+V+M++ +K +F R  G               SDFS    CEEALGVL + P+S++D  
Sbjct: 114  SMVEMNDGMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQFPISNEDKL 173

Query: 530  TILLLSKPECIKSMTIMLQRGSAQSRFYTISIIHKMTR-VDYDWKYIAEDQGIDFFKALL 706
               +  + E +KS+ +++QRGSA+ RFY + I+  + +  +Y+W  I E+QGIDFFK LL
Sbjct: 174  FDEMYKREELMKSIAVVVQRGSAEGRFYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLL 233

Query: 707  ELVSDEMGTKSSSYALQVLIDILTSSKKSRLKAIEAGAMCILIELLPDSNRSKCEKVLQL 886
            ELVSDE  TK+SS AL V+I+IL+SSK+SR+KAIEAGA+ ILIELLP+S RSKCE++LQ+
Sbjct: 234  ELVSDEFPTKASSCALDVMIEILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQI 293

Query: 887  IKLLCKSADGRLAFIEHGVGIGAISKKMLHVSLVSTKIVVKILWLICSFNPTNVRVIEDM 1066
            ++ LC+ A+GRLA +EHG+GI A++KK+L+VS V+TKI VKIL  I SF+P   RV+++M
Sbjct: 294  LQSLCECAEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRE-RVVDEM 352

Query: 1067 LFFGSVLKLEALLHID-------GGSSTKDIVVKILKLHGSSWMRYPCFPCDLKEFL 1216
            +  G+V KL  +LH+D       G SSTK+ V+KILK+HG+ W R PCFP +LK +L
Sbjct: 353  MACGAVKKLLMILHMDSTGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYL 409


>ref|XP_004155855.1| PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis sativus]
          Length = 365

 Score =  327 bits (839), Expect = 3e-87
 Identities = 183/367 (49%), Positives = 254/367 (69%), Gaps = 12/367 (3%)
 Frame = +2

Query: 152  MQTL----DNFEVTPNHTLKRLILSWRNSYCXXXXXXXXXXXIKHEEFVSLLNTIDSSCP 319
            MQTL    ++F +TPN  LKRLIL+W+ +                EE  SLL T++SS P
Sbjct: 1    MQTLAAGAEDFVITPNLNLKRLILAWKINDSALTPATAL------EEVSSLLATVESS-P 53

Query: 320  FKVSSLKKLRSIVQMSEEIKIDFKRCGGXXXXXXXXXXXXXXNSDFSIFRACEEALGVLH 499
            FKVSSL+KLRS+++M++ +K +F R  G               SDFS    CEEALGVL 
Sbjct: 54   FKVSSLRKLRSMIEMNDGMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLC 113

Query: 500  ELPLSDDDDETILLLSKPECIKSMTIMLQRGSAQSRFYTISIIHKMTR-VDYDWKYIAED 676
            + P+S++D     +  + E +KS+ +++QRGSA+ RFY + I+  + +  +Y+W  I E+
Sbjct: 114  QFPISNEDKLFDEMYKREELMKSIAVVVQRGSAEGRFYAVKILRNIAKNTNYNWGSIMEE 173

Query: 677  QGIDFFKALLELVSDEMGTKSSSYALQVLIDILTSSKKSRLKAIEAGAMCILIELLPDSN 856
            QGIDFFK LLELVSDE  TK+SS AL V+I+IL+SSK+SR+KAIEAGA+ ILIELLP+S 
Sbjct: 174  QGIDFFKPLLELVSDEFPTKASSCALDVMIEILSSSKRSRVKAIEAGAVWILIELLPESG 233

Query: 857  RSKCEKVLQLIKLLCKSADGRLAFIEHGVGIGAISKKMLHVSLVSTKIVVKILWLICSFN 1036
            RSKCE++LQ+++ LC+ A+GRLA +EHG+GI A++KK+L+VS V+TKI VKIL  I SF+
Sbjct: 234  RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFH 293

Query: 1037 PTNVRVIEDMLFFGSVLKLEALLHID-------GGSSTKDIVVKILKLHGSSWMRYPCFP 1195
            P   RV+++M+  G+V KL  +LH+D       G SSTK+ V+KILK+HG+ W R PCFP
Sbjct: 294  PRE-RVVDEMMACGAVKKLLMILHMDSAGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFP 352

Query: 1196 CDLKEFL 1216
             +LK +L
Sbjct: 353  SELKNYL 359


>ref|XP_002447013.1| hypothetical protein SORBIDRAFT_06g026870 [Sorghum bicolor]
            gi|241938196|gb|EES11341.1| hypothetical protein
            SORBIDRAFT_06g026870 [Sorghum bicolor]
          Length = 412

 Score =  327 bits (838), Expect = 4e-87
 Identities = 180/408 (44%), Positives = 259/408 (63%), Gaps = 8/408 (1%)
 Frame = +2

Query: 17   PLDFRCPISMELMKDPVTISTGVSYERKHIEKWINSYQKKTCPATMQTLDNFEVTPNHTL 196
            P  F CPISMELM+DPVT+STGV+Y+R+ IE+W   Y K TCPATMQ L++F++TPNHTL
Sbjct: 7    PQLFLCPISMELMEDPVTVSTGVTYDRRSIERWFFKYGKTTCPATMQRLNSFDLTPNHTL 66

Query: 197  KRLILSWRNSYCXXXXXXXXXXXIKHEEFVSLLNTIDSSCPFKVSSLKKLRSIVQMSEEI 376
            KR+I +W +              +  E+  S+L  I+++ PFKV++LK LR  +      
Sbjct: 67   KRVISTWLDRASSSSSSTPLCTKLAREKLPSVLAGIEAT-PFKVTALKNLRRCMDEDVAA 125

Query: 377  KIDFKRCGGXXXXXXXXXXXXXXNS---DFSIFRACEEALGVLHELPLSDDDDETILLLS 547
            + DF   GG              +    DFS FR CEEA  VL  LPLSDD   ++ L+ 
Sbjct: 126  QQDFVAYGGIQVLGRVMTQALAESCAGVDFSAFRTCEEAGAVLATLPLSDD--ASVELVL 183

Query: 548  KPECIKSMTIMLQRGSAQSRFYTISIIHKMTRV---DYDWKYIAEDQGIDFFKALLELVS 718
            KPEC+K +  ++QRGSA SR + ++I+ K++     D DW    +    D  K+LLEL+S
Sbjct: 184  KPECMKPVVAVVQRGSADSRLHAMAILAKISGASGADRDWTPGVDVD--DLVKSLLELLS 241

Query: 719  DEMGTKSSSYALQVLIDILTSSKKSR-LKAIEAGAMCILIELLPDSNRSKCEKVLQLIKL 895
            D    K SS AL VL+D+   S+ +R  KA+E GA+C+L+ELLPD++R   E+ L L+K 
Sbjct: 242  DGASAKLSSRALDVLLDVTARSRGARRAKAVEVGAVCVLVELLPDADRRVAERALLLLKR 301

Query: 896  LCKSADGRLAFIEHGVGIGAISKKMLHVSLVSTKIVVKILWLI-CSFNPTNVRVIEDMLF 1072
            LCK  +GRLAF EH + + A+++ ML VS++++++ V +LWL+ CS  P   RV++DML 
Sbjct: 302  LCKCPEGRLAFAEHALAVSAVARTMLRVSVLASRLAVSVLWLVACSATPAE-RVLDDMLV 360

Query: 1073 FGSVLKLEALLHIDGGSSTKDIVVKILKLHGSSWMRYPCFPCDLKEFL 1216
             G V KL ALL ++  +STK+   K+L++HG  W +YPCFP DL+++L
Sbjct: 361  SGGVGKLLALLQVENSASTKEKAAKLLRVHGPFWRQYPCFPTDLRDYL 408