BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00018093
         (1249 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002283468.1| PREDICTED: vacuolar amino acid transporter 1...   249   e-116
ref|XP_003633855.1| PREDICTED: vacuolar amino acid transporter 1...   250   e-116
ref|XP_002283458.2| PREDICTED: vacuolar amino acid transporter 1...   241   e-114
ref|XP_003633854.1| PREDICTED: vacuolar amino acid transporter 1...   241   e-113
ref|XP_002323861.1| amino acid transporter [Populus trichocarpa]...   243   e-111

>ref|XP_002283468.1| PREDICTED: vacuolar amino acid transporter 1 [Vitis vinifera]
            gi|296082904|emb|CBI22205.3| unnamed protein product
            [Vitis vinifera]
          Length = 428

 Score =  249 bits (637), Expect(2) = e-116
 Identities = 116/179 (64%), Positives = 149/179 (83%)
 Frame = +1

Query: 712  ILSYISATGVVAMLVILASIFWTGAFEGVGFHEKGELLNYKGIPTAVSLFAFCYCAHPVF 891
            ILSYISA+GV+A ++IL SI WTGAF+GVGF EKG  LN++GIPTAVSL+AFCYCAHPVF
Sbjct: 183  ILSYISASGVLACIIILGSILWTGAFDGVGFDEKGTSLNWQGIPTAVSLYAFCYCAHPVF 242

Query: 892  PTLYTSMENKKNFSMVMFLSFFLCSITYALIAVFGYLMFGSNLESQITLNLPVNKLSSKI 1071
            PTLYTSM  K  FS V+ + F  C+ITYA +A+ GYLMFGSN++SQITLNLP+ KLSS+I
Sbjct: 243  PTLYTSMRKKHQFSNVLLVCFIFCTITYAAMAILGYLMFGSNVQSQITLNLPIEKLSSRI 302

Query: 1072 AIYTILINPVAKFALIITPVMHSIENRLRSYSKKRSFSILIRTALLISIVTVALSIPFF 1248
            AIYT L+NP++K+AL++TP++ + EN L  Y KKR FS++IR+ L+ S + VAL++PFF
Sbjct: 303  AIYTTLVNPISKYALMVTPIVDATENWLPYYYKKRPFSLIIRSTLVFSTIIVALTVPFF 361



 Score =  198 bits (503), Expect(2) = e-116
 Identities = 101/176 (57%), Positives = 126/176 (71%)
 Frame = +2

Query: 77  MAAKLEDNYYLTVGLLQKNDKNAKQQEVNVGVVGESILENSFIGHSTEGTTSFFKTAFNA 256
           M A+  D + LT+ LL  +  + K +++      + +           G+TSFFKT FN 
Sbjct: 1   MEAEQRDGFTLTLPLLHDDQNHNKVEDIESNGRKQDV-----------GSTSFFKTCFNG 49

Query: 257 LNSLSGVGILSTPYALASGGWLSLILLFIIAMVTFYTALLIQRCMEADSTIKSYPDIGER 436
           LN+LSGVGILSTPYAL+SGGWLSL+LL +IA  TFYT LL++RCM+A   I++YPD+GER
Sbjct: 50  LNALSGVGILSTPYALSSGGWLSLVLLLVIATATFYTGLLLRRCMDAHPNIRTYPDVGER 109

Query: 437 AFGKKGRLIVLIFMNLELYLVATGFLILEGDNLTNLVPNMGFEINGFVFGGKQSFI 604
           AFGK GRL+V +FM  ELYLVATGFLILEGDNL NL PNMG EI G +  G+QSFI
Sbjct: 110 AFGKMGRLLVSVFMYTELYLVATGFLILEGDNLHNLFPNMGLEIWGLMIDGRQSFI 165


>ref|XP_003633855.1| PREDICTED: vacuolar amino acid transporter 1-like [Vitis vinifera]
            gi|296082909|emb|CBI22210.3| unnamed protein product
            [Vitis vinifera]
          Length = 417

 Score =  250 bits (639), Expect(2) = e-116
 Identities = 116/179 (64%), Positives = 150/179 (83%)
 Frame = +1

Query: 712  ILSYISATGVVAMLVILASIFWTGAFEGVGFHEKGELLNYKGIPTAVSLFAFCYCAHPVF 891
            ILSYISA+GV+A ++IL SI WTGAF+GVGF EKG  LN++GIPTAVSL+AFCYCAHPVF
Sbjct: 172  ILSYISASGVLACIIILGSILWTGAFDGVGFDEKGTSLNWQGIPTAVSLYAFCYCAHPVF 231

Query: 892  PTLYTSMENKKNFSMVMFLSFFLCSITYALIAVFGYLMFGSNLESQITLNLPVNKLSSKI 1071
            PTLYTSM  K  FS V+ + F  C+ITYA +A+ GYLMFGSN++SQITLNLP+ KLSS+I
Sbjct: 232  PTLYTSMRKKHQFSNVLLVCFIFCTITYAAMAILGYLMFGSNVQSQITLNLPIEKLSSRI 291

Query: 1072 AIYTILINPVAKFALIITPVMHSIENRLRSYSKKRSFSILIRTALLISIVTVALSIPFF 1248
            AIYT L+NP++K+AL++TP++++ EN L  Y KKR FS++IR+ L+ S + VAL++PFF
Sbjct: 292  AIYTTLVNPISKYALMVTPIVNATENWLPYYYKKRPFSLIIRSTLVFSTIIVALTVPFF 350



 Score =  194 bits (493), Expect(2) = e-116
 Identities = 94/133 (70%), Positives = 109/133 (81%)
 Frame = +2

Query: 206 GHSTEGTTSFFKTAFNALNSLSGVGILSTPYALASGGWLSLILLFIIAMVTFYTALLIQR 385
           G    G+TSFFKT FN LN+LSGVGILS PYAL+SGGWLSL+LL +IA  TFYT LL++R
Sbjct: 22  GKQDVGSTSFFKTCFNGLNALSGVGILSIPYALSSGGWLSLVLLLVIATATFYTGLLLRR 81

Query: 386 CMEADSTIKSYPDIGERAFGKKGRLIVLIFMNLELYLVATGFLILEGDNLTNLVPNMGFE 565
           CM+A   I++YPD+GERAFGKKGRL+V +FM  ELYLVATGFLILEGDNL NL PNMG E
Sbjct: 82  CMDAHPNIRTYPDVGERAFGKKGRLLVSVFMYTELYLVATGFLILEGDNLHNLFPNMGLE 141

Query: 566 INGFVFGGKQSFI 604
           I G +  G+QSFI
Sbjct: 142 IWGLMIDGRQSFI 154


>ref|XP_002283458.2| PREDICTED: vacuolar amino acid transporter 1-like [Vitis vinifera]
            gi|296082903|emb|CBI22204.3| unnamed protein product
            [Vitis vinifera]
          Length = 428

 Score =  241 bits (615), Expect(2) = e-114
 Identities = 112/179 (62%), Positives = 143/179 (79%)
 Frame = +1

Query: 712  ILSYISATGVVAMLVILASIFWTGAFEGVGFHEKGELLNYKGIPTAVSLFAFCYCAHPVF 891
            +LSYISA+GV A ++IL SI WTG F+GVGFH KG  LN+KGIPTA SL+AFCYCAHPVF
Sbjct: 183  MLSYISASGVFACIIILGSILWTGVFDGVGFHGKGTTLNWKGIPTAFSLYAFCYCAHPVF 242

Query: 892  PTLYTSMENKKNFSMVMFLSFFLCSITYALIAVFGYLMFGSNLESQITLNLPVNKLSSKI 1071
            PTLYTSM  K  FS V+ + F  C+ITYA +AV GYLMFGS ++SQITLNLP+ KLSS++
Sbjct: 243  PTLYTSMRKKNQFSTVLLVGFVFCTITYAAMAVLGYLMFGSEVQSQITLNLPIEKLSSRV 302

Query: 1072 AIYTILINPVAKFALIITPVMHSIENRLRSYSKKRSFSILIRTALLISIVTVALSIPFF 1248
            AIYT L+NP++K+ALI+ P++++ EN       +R FS+LIRTAL+ S + VAL++PFF
Sbjct: 303  AIYTTLVNPISKYALIVVPIVNATENWFPYCCNRRLFSLLIRTALVFSTIIVALTVPFF 361



 Score =  198 bits (504), Expect(2) = e-114
 Identities = 97/128 (75%), Positives = 111/128 (86%)
 Frame = +2

Query: 221 GTTSFFKTAFNALNSLSGVGILSTPYALASGGWLSLILLFIIAMVTFYTALLIQRCMEAD 400
           G+TSFFKT FN LN+LSG+GILS PYALASGGWLSL+LLF+IA+ TFYT LL+QRCM+ D
Sbjct: 38  GSTSFFKTCFNGLNALSGIGILSVPYALASGGWLSLMLLFVIALATFYTGLLLQRCMDVD 97

Query: 401 STIKSYPDIGERAFGKKGRLIVLIFMNLELYLVATGFLILEGDNLTNLVPNMGFEINGFV 580
             I++YPDIGE+AFGKKGRL+V IFM LELYLVATGFLILEGDNL NL P +GFEI G V
Sbjct: 98  LNIRTYPDIGEQAFGKKGRLMVSIFMYLELYLVATGFLILEGDNLHNLFPMVGFEIFGQV 157

Query: 581 FGGKQSFI 604
             G+QSFI
Sbjct: 158 IDGRQSFI 165


>ref|XP_003633854.1| PREDICTED: vacuolar amino acid transporter 1-like [Vitis vinifera]
          Length = 429

 Score =  241 bits (615), Expect(2) = e-113
 Identities = 113/180 (62%), Positives = 145/180 (80%), Gaps = 1/180 (0%)
 Frame = +1

Query: 712  ILSYISATGVVAMLVILASIFWTGAFEGVGFHEKGELLNYKGIPTAVSLFAFCYCAHPVF 891
            +LSYISA+GV A ++IL SI WTG F+GVGFH KG  LN+KGIPTA SL+AFCYCAHPVF
Sbjct: 183  MLSYISASGVFACIIILGSILWTGVFDGVGFHGKGTTLNWKGIPTAFSLYAFCYCAHPVF 242

Query: 892  PTLYTSMENKKNFSMVMFL-SFFLCSITYALIAVFGYLMFGSNLESQITLNLPVNKLSSK 1068
            PTLYTSM  K  FS V+ L  F  C+I YA +AV GYLMFGS ++SQITLNLP+ KLSS+
Sbjct: 243  PTLYTSMRKKNQFSTVILLVCFIFCTIAYAAMAVLGYLMFGSEVQSQITLNLPIEKLSSR 302

Query: 1069 IAIYTILINPVAKFALIITPVMHSIENRLRSYSKKRSFSILIRTALLISIVTVALSIPFF 1248
            +AIYT L+NP++K+AL++ P++++ EN   +Y  +RSFS+LIRTAL+ S + VAL++PFF
Sbjct: 303  VAIYTTLVNPISKYALMVAPIVNATENCFPNYCNRRSFSLLIRTALVFSTIIVALTVPFF 362



 Score =  195 bits (496), Expect(2) = e-113
 Identities = 105/177 (59%), Positives = 125/177 (70%), Gaps = 1/177 (0%)
 Frame = +2

Query: 77  MAAKLEDNYYLTVGLLQKNDKNAKQQEVNVGVVGESILENSFIGHSTE-GTTSFFKTAFN 253
           MA +  D + L   LL    +N +             +E S  G   + G+TSFFKT FN
Sbjct: 1   MAPEQLDGFSLAFPLLHDESQNVED------------MEYSSTGCKLDLGSTSFFKTCFN 48

Query: 254 ALNSLSGVGILSTPYALASGGWLSLILLFIIAMVTFYTALLIQRCMEADSTIKSYPDIGE 433
            LN+L G+GILS PYALASGGWLSL+LLF+I + TFYT LL+QRCM+ D  I++YPDIGE
Sbjct: 49  GLNALLGIGILSVPYALASGGWLSLMLLFVITLATFYTGLLLQRCMDVDLNIRTYPDIGE 108

Query: 434 RAFGKKGRLIVLIFMNLELYLVATGFLILEGDNLTNLVPNMGFEINGFVFGGKQSFI 604
           +AFGKKGRL+V IFM LELYLVATGFLILEGDNL NL P +GFEI G V  G+QSFI
Sbjct: 109 QAFGKKGRLMVSIFMYLELYLVATGFLILEGDNLHNLFPMVGFEIFGQVIDGRQSFI 165


>ref|XP_002323861.1| amino acid transporter [Populus trichocarpa]
            gi|222866863|gb|EEF03994.1| amino acid transporter
            [Populus trichocarpa]
          Length = 388

 Score =  243 bits (619), Expect(2) = e-111
 Identities = 117/179 (65%), Positives = 147/179 (82%)
 Frame = +1

Query: 712  ILSYISATGVVAMLVILASIFWTGAFEGVGFHEKGELLNYKGIPTAVSLFAFCYCAHPVF 891
            ILSYISA+GV+A  ++L SIFWTGAF+GVGF EKG L+N+ GIPTAVSL+AFCYCAHPVF
Sbjct: 146  ILSYISASGVIASAIVLISIFWTGAFDGVGFKEKGTLVNWHGIPTAVSLYAFCYCAHPVF 205

Query: 892  PTLYTSMENKKNFSMVMFLSFFLCSITYALIAVFGYLMFGSNLESQITLNLPVNKLSSKI 1071
            PTLYTSM+NK  FS VM L F LCS++YA +AV GYLMFG +++SQITLNLP  KLSSK+
Sbjct: 206  PTLYTSMKNKHQFSNVMILCFILCSLSYASMAVMGYLMFGPSVQSQITLNLPTEKLSSKL 265

Query: 1072 AIYTILINPVAKFALIITPVMHSIENRLRSYSKKRSFSILIRTALLISIVTVALSIPFF 1248
             IYT L++P+ K+AL+I P++++ ++ L    KKR FS+ I TAL+IS V VALS+PFF
Sbjct: 266  TIYTTLVSPITKYALMIIPIVNATKSWLPMNGKKRPFSLFIGTALVISNVIVALSLPFF 324



 Score =  187 bits (475), Expect(2) = e-111
 Identities = 90/128 (70%), Positives = 106/128 (82%)
 Frame = +2

Query: 221 GTTSFFKTAFNALNSLSGVGILSTPYALASGGWLSLILLFIIAMVTFYTALLIQRCMEAD 400
           G+TS FKT F+ LN+LSGVGILS PYAL+SGGWLSLILL +I+   FYT LLIQRCM+ D
Sbjct: 1   GSTSTFKTVFHGLNALSGVGILSIPYALSSGGWLSLILLCVISTAAFYTGLLIQRCMDLD 60

Query: 401 STIKSYPDIGERAFGKKGRLIVLIFMNLELYLVATGFLILEGDNLTNLVPNMGFEINGFV 580
           S I++YPDIGERAFGKKGR++V + M  ELYLV+TGFLILEGDNL NL PNMG E+ GF 
Sbjct: 61  SNIRTYPDIGERAFGKKGRILVSVLMYTELYLVSTGFLILEGDNLQNLFPNMGIEVAGFE 120

Query: 581 FGGKQSFI 604
            GG+QS +
Sbjct: 121 IGGRQSSV 128