BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00018034
         (347 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002532719.1| ubiquitin-protein ligase, putative [Ricinus ...    75   6e-12
ref|XP_002308787.1| f-box family protein [Populus trichocarpa] g...    75   7e-12
ref|XP_002297705.1| predicted protein [Populus trichocarpa] gi|2...    70   2e-10
ref|XP_002520466.1| ubiquitin-protein ligase, putative [Ricinus ...    64   1e-08
ref|NP_189030.1| F-box/kelch-repeat protein [Arabidopsis thalian...    63   2e-08

>ref|XP_002532719.1| ubiquitin-protein ligase, putative [Ricinus communis]
           gi|223527546|gb|EEF29668.1| ubiquitin-protein ligase,
           putative [Ricinus communis]
          Length = 389

 Score = 75.1 bits (183), Expect = 6e-12
 Identities = 49/123 (39%), Positives = 66/123 (53%), Gaps = 10/123 (8%)
 Frame = +2

Query: 5   MVPSAPAEFPRSFSMIE--TSLCGFGYDEVNDDYKVVKIAECYVQFEGI-------KVIV 157
           +VP  P E  R F M     S+ GFGYD  NDDYK+V+IA    QF G+       +V V
Sbjct: 120 VVPYLPVELKRYFGMCSCRVSVFGFGYDLSNDDYKLVRIA----QFGGVDRKSFESEVKV 175

Query: 158 YSLRNNLWTWFNKDFPDDTHFTGKCGMFVGGALHWLAIKN-SWNCRETIVRFDLGLEQFK 334
           +SLR N W     D P    + G+ G++  GALHWL  ++      +TIV  DLG+E + 
Sbjct: 176 FSLRKNSWRRI-ADMPYCVLYPGENGIYANGALHWLVSQDPDSTVADTIVALDLGVEDYH 234

Query: 335 ELP 343
            +P
Sbjct: 235 VVP 237


>ref|XP_002308787.1| f-box family protein [Populus trichocarpa]
           gi|222854763|gb|EEE92310.1| f-box family protein
           [Populus trichocarpa]
          Length = 396

 Score = 74.7 bits (182), Expect = 7e-12
 Identities = 49/122 (40%), Positives = 63/122 (51%), Gaps = 10/122 (8%)
 Frame = +2

Query: 5   MVPSAPAEFPRSFSMIETSLC--GFGYDEVNDDYKVVKIAECYVQFEG-------IKVIV 157
           +VP  P E  R F     S+   GFGYD V DDYK+V+IA    QF G        +V V
Sbjct: 127 VVPFLPIELKRYFGTKSCSVYVFGFGYDSVRDDYKLVRIA----QFGGGGKRSFESEVKV 182

Query: 158 YSLRNNLWTWFNKDFPDDTHFTGKCGMFVGGALHWLAIKN-SWNCRETIVRFDLGLEQFK 334
           YSLR   W     D P   H+ G  G+F  GALHW+  +N   N    +V  DLG+E ++
Sbjct: 183 YSLRKQSWRRIG-DMPYCVHYPGANGVFANGALHWVVGENPESNVANIVVALDLGVEDYR 241

Query: 335 EL 340
           E+
Sbjct: 242 EV 243


>ref|XP_002297705.1| predicted protein [Populus trichocarpa] gi|222844963|gb|EEE82510.1|
           predicted protein [Populus trichocarpa]
          Length = 408

 Score = 70.1 bits (170), Expect = 2e-10
 Identities = 48/134 (35%), Positives = 66/134 (49%), Gaps = 21/134 (15%)
 Frame = +2

Query: 5   MVPSAPAEFPRSFSMIETS----LCGFGYDEVNDDYKVVKIAECYVQ----FEGIKVIVY 160
           M+P +P E P      + S      GFG+D +N+DYKVV+    Y      F   +V VY
Sbjct: 122 MIPVSPLELPNDLDDSKVSSLFNFYGFGHDPINEDYKVVRFIHFYGDSPDGFFHCEVKVY 181

Query: 161 SLRNNLWTWFNKDFPDDTHFT------GKC----GMFVGGALHWLAI---KNSWNCRETI 301
           SL++N W   + D+P D  F        +C    G+F   A+HW A    K   N  + I
Sbjct: 182 SLKSNSWKRID-DYPYDLRFILPPDYHPRCRRGYGVFANSAVHWKATVVGKGKENGSDLI 240

Query: 302 VRFDLGLEQFKELP 343
           V FDLG E+FK +P
Sbjct: 241 VAFDLGAEEFKIIP 254


>ref|XP_002520466.1| ubiquitin-protein ligase, putative [Ricinus communis]
           gi|223540308|gb|EEF41879.1| ubiquitin-protein ligase,
           putative [Ricinus communis]
          Length = 358

 Score = 64.3 bits (155), Expect = 1e-08
 Identities = 37/99 (37%), Positives = 58/99 (58%), Gaps = 2/99 (2%)
 Frame = +2

Query: 50  IETSLCGFGYDEVNDDYKVVKIAECYVQFEGIKVIVYSLRNNLWTWFNKDFPDDTHFTG- 226
           ++  + G GYD  +DDYKVV+I +C  + +G+   +YSLR+N WT  + DFP D +    
Sbjct: 143 LQYDVFGLGYDAASDDYKVVRIQKCRSKKDGVG--IYSLRSNSWTRLH-DFPCDNYEFDW 199

Query: 227 -KCGMFVGGALHWLAIKNSWNCRETIVRFDLGLEQFKEL 340
              G  V G L+WL  K +++   +I+ FD+  E+F  L
Sbjct: 200 TAMGKHVSGTLYWLCAKETYSV--SIIAFDILTEKFHAL 236


>ref|NP_189030.1| F-box/kelch-repeat protein [Arabidopsis thaliana]
           gi|75273451|sp|Q9LIR8.1|FBK67_ARATH RecName:
           Full=F-box/kelch-repeat protein At3g23880
           gi|9294656|dbj|BAB03005.1| unnamed protein product
           [Arabidopsis thaliana] gi|26449693|dbj|BAC41970.1|
           unknown protein [Arabidopsis thaliana]
           gi|30725382|gb|AAP37713.1| At3g23880 [Arabidopsis
           thaliana] gi|332643304|gb|AEE76825.1| F-box/kelch-repeat
           protein [Arabidopsis thaliana]
          Length = 364

 Score = 63.2 bits (152), Expect = 2e-08
 Identities = 38/96 (39%), Positives = 53/96 (55%), Gaps = 4/96 (4%)
 Frame = +2

Query: 68  GFGYDEVNDDYKVVKIAECYVQFEGIKVIVYSLRNNLWTWFNKDFPDDTHFTGK--CGMF 241
           GFGYDE  DDYKVV + +   Q + I+  +YS R  LW   N  FP       K   G++
Sbjct: 160 GFGYDESEDDYKVVALLQQRHQVK-IETKIYSTRQKLWR-SNTSFPSGVVVADKSRSGIY 217

Query: 242 VGGALHWLAI--KNSWNCRETIVRFDLGLEQFKELP 343
           + G L+W A    +SW    TI+ +D+  ++FKELP
Sbjct: 218 INGTLNWAATSSSSSW----TIISYDMSRDEFKELP 249