BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00018031
         (587 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACF17642.1| putative branched-chain alpha-keto acid dehydroge...   169   2e-40
ref|XP_002270598.2| PREDICTED: lipoamide acyltransferase compone...   160   1e-37
ref|XP_003529559.1| PREDICTED: lipoamide acyltransferase compone...   157   2e-36
ref|XP_003550355.1| PREDICTED: lipoamide acyltransferase compone...   155   5e-36
emb|CBI22978.3| unnamed protein product [Vitis vinifera]              155   6e-36

>gb|ACF17642.1| putative branched-chain alpha-keto acid dehydrogenase E2 subunit
           [Capsicum annuum]
          Length = 505

 Score =  169 bits (429), Expect = 2e-40
 Identities = 90/182 (49%), Positives = 123/182 (67%), Gaps = 10/182 (5%)
 Frame = +1

Query: 52  RLLHRNPWRSAGWRWLLHTYTVADTSPLSPETAA---------RSSLLFIRNFTSML-SY 201
           R ++++  R++  R  +  +  +  +PL+P             +S L F RN T+   SY
Sbjct: 4   RKIYQSKLRNSVVRQSICRWFQSSVAPLTPAATVCSGSSGKRIQSQLHFFRNSTTAFCSY 63

Query: 202 SAQDFNFAKSPYGIKGCYFSTQPMSEVRVDGIVEIPLAQTGEGIAECELLKWFVKSGDHV 381
           +  + +F      ++GC F+TQ   ++   G+++IPLAQTGEGIAECELLKWFV+ GD V
Sbjct: 64  NKPNVSFT-----LRGCSFTTQAAIDLPAGGVIDIPLAQTGEGIAECELLKWFVQEGDLV 118

Query: 382 EEFQPLCEVQSDKATIEITSRYKGEVSEVLHVPGDIVKVGETLLKLFVSEVSVPAQTCDT 561
           EEFQPLCEVQSDKATIEITSRYKG++S++LHVPGDIVKVGETLLK+ + E+  P +T D 
Sbjct: 119 EEFQPLCEVQSDKATIEITSRYKGKISQILHVPGDIVKVGETLLKIGIDEIPDPIETSDA 178

Query: 562 LE 567
            E
Sbjct: 179 SE 180


>ref|XP_002270598.2| PREDICTED: lipoamide acyltransferase component of branched-chain
           alpha-keto acid dehydrogenase complex,
           mitochondrial-like [Vitis vinifera]
          Length = 506

 Score =  160 bits (405), Expect = 1e-37
 Identities = 92/173 (53%), Positives = 111/173 (64%), Gaps = 14/173 (8%)
 Frame = +1

Query: 55  LLHRNPW----RSAGWRWLLHTYTVADTSPLSPE---TAARSSLLFIRNFTSMLSYSAQD 213
           ++ R  W    R+A  RWL      A TSPLSP    +   SS +   +   +  Y+   
Sbjct: 1   MISRGIWQQKCRNAIRRWLRSC--AAQTSPLSPSPVVSLGNSSYIGFCSQPIVSRYAMAS 58

Query: 214 FNFAKS-------PYGIKGCYFSTQPMSEVRVDGIVEIPLAQTGEGIAECELLKWFVKSG 372
           F+           PY IK   FS+  + ++   GIV IPLAQTGEGIAECELLKWFVK G
Sbjct: 59  FSMVNDKLMDLNIPYSIKRSCFSSHALLDLPASGIVSIPLAQTGEGIAECELLKWFVKEG 118

Query: 373 DHVEEFQPLCEVQSDKATIEITSRYKGEVSEVLHVPGDIVKVGETLLKLFVSE 531
           D VEEFQPLCEVQSDKATIEITSRYKG VS++++VPGDIVKVGE+LLK+ V E
Sbjct: 119 DQVEEFQPLCEVQSDKATIEITSRYKGTVSQIIYVPGDIVKVGESLLKMVVEE 171


>ref|XP_003529559.1| PREDICTED: lipoamide acyltransferase component of branched-chain
           alpha-keto acid dehydrogenase complex,
           mitochondrial-like [Glycine max]
          Length = 515

 Score =  157 bits (396), Expect = 2e-36
 Identities = 91/175 (52%), Positives = 117/175 (66%), Gaps = 1/175 (0%)
 Frame = +1

Query: 40  MLSHRLLHRNPWRSAGWRWLLHTYTVADTSPLSPETAARSSLLFIRNFTSMLSYSAQDFN 219
           MLS R+  R    SA     L + + + +S  SP    R S     + + ++      FN
Sbjct: 1   MLSSRIWQRRALISAR---TLFSASASHSSSSSPAIPFRFSKANASSSSHLI------FN 51

Query: 220 FAKSPYGIKGCYFSTQPMSEVRVDGIVEIPLAQTGEGIAECELLKWFVKSGDHVEEFQPL 399
           FA+    +   +FSTQP  E+    IV++PLAQTGEGIAECELLKW+V+ GD+VE+FQPL
Sbjct: 52  FAR----VNIYWFSTQPALELPASKIVDVPLAQTGEGIAECELLKWYVQEGDYVEDFQPL 107

Query: 400 CEVQSDKATIEITSRYKGEVSEVLHVPGDIVKVGETLLKLFVSEVSVPAQT-CDT 561
           CEVQSDKATIEITSRYKG++S  L+VPGDIVKVGETLLK+ V E + P+ T CD+
Sbjct: 108 CEVQSDKATIEITSRYKGKISSFLYVPGDIVKVGETLLKILVDESAFPSGTPCDS 162


>ref|XP_003550355.1| PREDICTED: lipoamide acyltransferase component of branched-chain
           alpha-keto acid dehydrogenase complex,
           mitochondrial-like [Glycine max]
          Length = 515

 Score =  155 bits (392), Expect = 5e-36
 Identities = 77/117 (65%), Positives = 95/117 (81%), Gaps = 1/117 (0%)
 Frame = +1

Query: 214 FNFAKSPYGIKGCYFSTQPMSEVRVDGIVEIPLAQTGEGIAECELLKWFVKSGDHVEEFQ 393
           FNFA+    +   +FSTQP  E+    IV++PLAQTGEGIAECELLKW+V+ GD+VE+FQ
Sbjct: 50  FNFAR----VDRYWFSTQPALELPASKIVDVPLAQTGEGIAECELLKWYVQEGDYVEDFQ 105

Query: 394 PLCEVQSDKATIEITSRYKGEVSEVLHVPGDIVKVGETLLKLFVSEVSVPAQ-TCDT 561
           PLCEVQSDKATIEITSRYKG++S +L+VPGDIVKVGETLLK+ V E + P+   CD+
Sbjct: 106 PLCEVQSDKATIEITSRYKGKISNILYVPGDIVKVGETLLKILVDESTFPSGIPCDS 162


>emb|CBI22978.3| unnamed protein product [Vitis vinifera]
          Length = 463

 Score =  155 bits (391), Expect = 6e-36
 Identities = 78/115 (67%), Positives = 90/115 (78%)
 Frame = +1

Query: 187 SMLSYSAQDFNFAKSPYGIKGCYFSTQPMSEVRVDGIVEIPLAQTGEGIAECELLKWFVK 366
           SM++    D N    PY IK   FS+  + ++   GIV IPLAQTGEGIAECELLKWFVK
Sbjct: 5   SMVNDKLMDLNI---PYSIKRSCFSSHALLDLPASGIVSIPLAQTGEGIAECELLKWFVK 61

Query: 367 SGDHVEEFQPLCEVQSDKATIEITSRYKGEVSEVLHVPGDIVKVGETLLKLFVSE 531
            GD VEEFQPLCEVQSDKATIEITSRYKG VS++++VPGDIVKVGE+LLK+ V E
Sbjct: 62  EGDQVEEFQPLCEVQSDKATIEITSRYKGTVSQIIYVPGDIVKVGESLLKMVVEE 116