BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00018022
         (793 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   320   3e-85
ref|XP_003521174.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   277   2e-72
ref|XP_002308030.1| predicted protein [Populus trichocarpa] gi|2...   268   1e-69
ref|XP_003553411.1| PREDICTED: LOW QUALITY PROTEIN: cytoplasmic ...   249   4e-64
ref|NP_567993.1| cytoplasmic tRNA 2-thiolation protein 2 [Arabid...   249   6e-64

>ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Vitis vinifera]
           gi|297739656|emb|CBI29838.3| unnamed protein product
           [Vitis vinifera]
          Length = 468

 Score =  320 bits (819), Expect = 3e-85
 Identities = 159/222 (71%), Positives = 182/222 (81%), Gaps = 1/222 (0%)
 Frame = -3

Query: 791 IAATVKGQGYSLAADIQYVDARWDIPVVHPLRDCITQELNMLCRLDSLKTLELYNGSRSG 612
           I+ATVKGQGYSL ADIQYVDARW+IPVV PLRDC+ QELNMLCRLD LKT+E+ N   SG
Sbjct: 248 ISATVKGQGYSLPADIQYVDARWEIPVVLPLRDCLAQELNMLCRLDGLKTMEVLNSPHSG 307

Query: 611 INGLISSFVKLLQEENPSRECTIVRTAGKLTPFHFNKIPEEADDCNSQLASQRRRKKFNL 432
           INGL++SFV LLQEENPSRECTIVRTAGKLTPFHFN+IP E+DD N  LAS+RR+KKFNL
Sbjct: 308 INGLVASFVTLLQEENPSRECTIVRTAGKLTPFHFNQIP-ESDDSNVHLASRRRQKKFNL 366

Query: 431 KTNELLPPESFCPICNSPLDRKNLCNL-TFESGFTSSEIFGSKCCSSCQFQILPKEPVDM 255
           K NE +PPES+CPICNSPL + +  NL +  S  T+++IFG+ CC SCQFQILPKEPV M
Sbjct: 367 KPNESVPPESYCPICNSPLSKSDHINLSSLGSDQTNADIFGATCCPSCQFQILPKEPVSM 426

Query: 254 EHFYSLLPQSIIDRVKDGSCGNRRSTREQIHDCLLSDSEDET 129
           EHFYSLLPQ  + + KD S   +R  REQI D LLSDSED T
Sbjct: 427 EHFYSLLPQQFVVQAKDRSFSMQRQLREQIQDFLLSDSEDGT 468


>ref|XP_003521174.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Glycine
           max]
          Length = 458

 Score =  277 bits (708), Expect = 2e-72
 Identities = 139/221 (62%), Positives = 167/221 (75%)
 Frame = -3

Query: 791 IAATVKGQGYSLAADIQYVDARWDIPVVHPLRDCITQELNMLCRLDSLKTLELYNGSRSG 612
           I+ATVKGQGYSL ADIQYVDARW++PVV PLRDC  QE+NMLC LD LKT++L     S 
Sbjct: 239 ISATVKGQGYSLPADIQYVDARWEVPVVLPLRDCFAQEINMLCHLDGLKTVKLSTDLCSS 298

Query: 611 INGLISSFVKLLQEENPSRECTIVRTAGKLTPFHFNKIPEEADDCNSQLASQRRRKKFNL 432
           INGL+SSFV LLQEENPSRE TIVRTAGKLTPF FN+IPE  D  N  LA++RR+K++NL
Sbjct: 299 INGLVSSFVALLQEENPSRESTIVRTAGKLTPFQFNRIPEIIDG-NVPLATRRRQKRYNL 357

Query: 431 KTNELLPPESFCPICNSPLDRKNLCNLTFESGFTSSEIFGSKCCSSCQFQILPKEPVDME 252
           K+NE +  ESFCP+CNSPLD+  + + +      SS+IF + CCSSC+FQILP + + ME
Sbjct: 358 KSNESVSSESFCPLCNSPLDKSEITDWSNIDSHRSSDIFYNTCCSSCRFQILPSDSMAME 417

Query: 251 HFYSLLPQSIIDRVKDGSCGNRRSTREQIHDCLLSDSEDET 129
            FY  LPQS++ R K  + GN    REQI D LLSD EDET
Sbjct: 418 QFYMDLPQSVVGRAKQANNGNLSLLREQIQDYLLSDGEDET 458


>ref|XP_002308030.1| predicted protein [Populus trichocarpa] gi|222854006|gb|EEE91553.1|
           predicted protein [Populus trichocarpa]
          Length = 443

 Score =  268 bits (684), Expect = 1e-69
 Identities = 131/201 (65%), Positives = 159/201 (79%)
 Frame = -3

Query: 791 IAATVKGQGYSLAADIQYVDARWDIPVVHPLRDCITQELNMLCRLDSLKTLELYNGSRSG 612
           +AATVKGQGYSL+ADIQYVDARW+IPVV PLRDC +QELN+LC+LD LKTL+ ++ + SG
Sbjct: 240 LAATVKGQGYSLSADIQYVDARWEIPVVLPLRDCTSQELNLLCQLDGLKTLQPFDSTPSG 299

Query: 611 INGLISSFVKLLQEENPSRECTIVRTAGKLTPFHFNKIPEEADDCNSQLASQRRRKKFNL 432
           INGLISSFV +LQEEN SRECTI+RTAGKLTPF FN+IP E D+CN  LA +RR+K++NL
Sbjct: 300 INGLISSFVNVLQEENSSRECTILRTAGKLTPFEFNRIP-ETDNCNVPLAIRRRQKRYNL 358

Query: 431 KTNELLPPESFCPICNSPLDRKNLCNLTFESGFTSSEIFGSKCCSSCQFQILPKEPVDME 252
           KT E +  E FCPICN+PL   N  NL+      SS  F + CCSSCQFQI+PK+P  M 
Sbjct: 359 KTKESISSELFCPICNAPLKNFNSLNLSSLKSCQSSR-FNAACCSSCQFQIIPKDPSSMG 417

Query: 251 HFYSLLPQSIIDRVKDGSCGN 189
           +F SLLPQ ++DR K G+C N
Sbjct: 418 NFCSLLPQQVVDRAKHGNCSN 438


>ref|XP_003553411.1| PREDICTED: LOW QUALITY PROTEIN: cytoplasmic tRNA 2-thiolation
           protein 2-like, partial [Glycine max]
          Length = 389

 Score =  249 bits (637), Expect = 4e-64
 Identities = 123/201 (61%), Positives = 151/201 (75%)
 Frame = -3

Query: 791 IAATVKGQGYSLAADIQYVDARWDIPVVHPLRDCITQELNMLCRLDSLKTLELYNGSRSG 612
           I+ATVKGQGYSL ADIQYVDARW++PVV PLRDC  QE+NMLCRLD LKT++L     S 
Sbjct: 185 ISATVKGQGYSLPADIQYVDARWEVPVVLPLRDCFAQEINMLCRLDGLKTVKLSTDLCSS 244

Query: 611 INGLISSFVKLLQEENPSRECTIVRTAGKLTPFHFNKIPEEADDCNSQLASQRRRKKFNL 432
           INGL+SSFV LLQEENPSRE +IVRTAGKLTPF FN+IPE  D  N  L ++R +K++NL
Sbjct: 245 INGLVSSFVALLQEENPSRESSIVRTAGKLTPFQFNRIPEIIDS-NVPLTTRRCQKRYNL 303

Query: 431 KTNELLPPESFCPICNSPLDRKNLCNLTFESGFTSSEIFGSKCCSSCQFQILPKEPVDME 252
           K+NE +  ESFCP+CNSPLD+  + + +      SS+IF + CCSSC+FQILP +   ME
Sbjct: 304 KSNESVSSESFCPLCNSPLDKSEIVDWSNIDSHRSSDIFYNTCCSSCRFQILPSDSTTME 363

Query: 251 HFYSLLPQSIIDRVKDGSCGN 189
            FY   PQS++ R K  + GN
Sbjct: 364 QFYMDFPQSVVSRPKQANNGN 384


>ref|NP_567993.1| cytoplasmic tRNA 2-thiolation protein 2 [Arabidopsis thaliana]
           gi|332278212|sp|O65628.3|CTU2_ARATH RecName:
           Full=Cytoplasmic tRNA 2-thiolation protein 2
           gi|115646788|gb|ABJ17118.1| At4g35910 [Arabidopsis
           thaliana] gi|332661188|gb|AEE86588.1| cytoplasmic tRNA
           2-thiolation protein 2 [Arabidopsis thaliana]
          Length = 458

 Score =  249 bits (635), Expect = 6e-64
 Identities = 127/220 (57%), Positives = 160/220 (72%), Gaps = 1/220 (0%)
 Frame = -3

Query: 791 IAATVKGQGYSLAADIQYVDARWDIPVVHPLRDCITQELNMLCRLDSLKTLELYNGSRSG 612
           + ATVKG+GYSL+ADIQ+VDARW +P+V PLRDC+  E+  LC LD LKT+EL   S+ G
Sbjct: 239 LTATVKGRGYSLSADIQHVDARWKVPIVLPLRDCVRLEITRLCLLDGLKTVELACRSQCG 298

Query: 611 INGLISSFVKLLQEENPSRECTIVRTAGKLTPFHFNKIPEEADDCNSQLASQRRRKKFNL 432
           IN L+SSFV LLQEENPSRECTIVRTA KLTPF+FNKIP E DD N  +A+QRR K+FNL
Sbjct: 299 INDLVSSFVALLQEENPSRECTIVRTAAKLTPFYFNKIP-ETDDSNVPMATQRRLKRFNL 357

Query: 431 KTNELLPPESFCPICNSPLDRKNLCNL-TFESGFTSSEIFGSKCCSSCQFQILPKEPVDM 255
           K +  +  E+FCPICN PL+R +   L TFE G   S++  + CCSSC+FQILP++   +
Sbjct: 358 KYDGSMTTEAFCPICNGPLNRSDSSELDTFEEG-QESDVLYAACCSSCRFQILPQDGSSL 416

Query: 254 EHFYSLLPQSIIDRVKDGSCGNRRSTREQIHDCLLSDSED 135
           E F S LP  +I +VK     ++   RE+I DCLL D E+
Sbjct: 417 EQFSSFLPDHMISQVKHQKVDSQAYLREKIKDCLLLDDEE 456