BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00017991
         (671 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFO67231.1| putative cytochrome P450, partial [Aralia elata]       220   2e-55
dbj|BAL45198.1| cytochrome P450 monooxygenase [Medicago truncatula]   203   3e-50
gb|ABC59078.1| cytochrome P450 monooxygenase CYP72A59 [Medicago ...   202   4e-50
ref|XP_003543244.1| PREDICTED: secologanin synthase-like [Glycin...   200   2e-49
ref|XP_003634731.1| PREDICTED: LOW QUALITY PROTEIN: secologanin ...   199   4e-49

>gb|AFO67231.1| putative cytochrome P450, partial [Aralia elata]
          Length = 155

 Score =  220 bits (560), Expect = 2e-55
 Identities = 100/142 (70%), Positives = 121/142 (85%)
 Frame = -1

Query: 584 KIYNRLWVRPKQMEKQLKELGFKGNPYRFLHGDMKDFFSIAAQANSKPINFTNDIRSRVL 405
           K+ NRLWVRPK++EK L+ELGF GNPY+FLHGDMK+F  I+  A SKP++F++D+ +RVL
Sbjct: 14  KLVNRLWVRPKKIEKYLRELGFSGNPYKFLHGDMKEFSRISEAAMSKPMDFSHDVGARVL 73

Query: 404 PYVHHVVNKHGKSSFVWFGAKAKLNITDPTLVKEILLKFEEFHKVYPDPVADLTVGGLST 225
           PY HH+V K+G +SF+WFGAK +LNI DP LVKEIL K +EFHKVYPDPVADL VGGLST
Sbjct: 74  PYHHHLVKKYGNNSFMWFGAKPRLNIMDPVLVKEILSKPDEFHKVYPDPVADLVVGGLST 133

Query: 224 AHGETWPRHRKILNATFSLEKL 159
           AHGE WPRHRKI+N  F+LEKL
Sbjct: 134 AHGEKWPRHRKIINQAFNLEKL 155


>dbj|BAL45198.1| cytochrome P450 monooxygenase [Medicago truncatula]
          Length = 518

 Score =  203 bits (516), Expect = 3e-50
 Identities = 95/194 (48%), Positives = 132/194 (68%)
 Frame = -1

Query: 584 KIYNRLWVRPKQMEKQLKELGFKGNPYRFLHGDMKDFFSIAAQANSKPINFTNDIRSRVL 405
           +I N LW++PK++EK L+E G KGN YR L GD+KD   I  +A +KP+N ++DI  RV 
Sbjct: 22  RILNWLWLKPKKLEKLLREQGLKGNSYRLLVGDIKDLLKIRKEATTKPMNLSDDIVPRVY 81

Query: 404 PYVHHVVNKHGKSSFVWFGAKAKLNITDPTLVKEILLKFEEFHKVYPDPVADLTVGGLST 225
            +VH  V KHGK+SF+WFG   ++NITDP  +K++L K  +F K   +P+  L   GL +
Sbjct: 82  SFVHQSVTKHGKNSFIWFGPTPRVNITDPDQIKDVLNKIYDFQKPNANPLVRLLANGLVS 141

Query: 224 AHGETWPRHRKILNATFSLEKLKGTLPDIYLSCQDTISKWKALLVSTAGNTEIDAWPYMK 45
             GE W +HRKI+N  F LEKLK  LP  + SC D ISKW+ +L S+ G+ E+D WP+++
Sbjct: 142 LEGEQWSKHRKIINPAFHLEKLKIMLPIFHKSCDDMISKWEKML-SSDGSCEMDVWPFLQ 200

Query: 44  NLARDMLSRVAFGS 3
           N A D++SR AFGS
Sbjct: 201 NFASDVISRAAFGS 214


>gb|ABC59078.1| cytochrome P450 monooxygenase CYP72A59 [Medicago truncatula]
          Length = 518

 Score =  202 bits (515), Expect = 4e-50
 Identities = 95/194 (48%), Positives = 131/194 (67%)
 Frame = -1

Query: 584 KIYNRLWVRPKQMEKQLKELGFKGNPYRFLHGDMKDFFSIAAQANSKPINFTNDIRSRVL 405
           +I N LW++PK++EK L+E G KGN YR L GDM D   +  +A SKP+N ++DI  RV 
Sbjct: 22  RILNWLWLKPKKLEKLLREQGLKGNSYRLLVGDMNDLLKMRKEATSKPMNLSDDIVPRVY 81

Query: 404 PYVHHVVNKHGKSSFVWFGAKAKLNITDPTLVKEILLKFEEFHKVYPDPVADLTVGGLST 225
            +VH  V KHGK+SF+WFG   ++NITDP  +K++L K  +F K   +P+  L   GL +
Sbjct: 82  SFVHQSVTKHGKNSFIWFGPTPRVNITDPDQIKDVLNKIYDFQKPNANPLVRLLANGLVS 141

Query: 224 AHGETWPRHRKILNATFSLEKLKGTLPDIYLSCQDTISKWKALLVSTAGNTEIDAWPYMK 45
             GE W +HRKI+N  F LEKLK  LP  + SC D ISKW+ +L S+ G+ E+D WP+++
Sbjct: 142 LEGEQWSKHRKIINPAFHLEKLKIMLPIFHKSCDDLISKWEKML-SSDGSCEMDVWPFLQ 200

Query: 44  NLARDMLSRVAFGS 3
           N A D++SR AFGS
Sbjct: 201 NFASDVISRAAFGS 214


>ref|XP_003543244.1| PREDICTED: secologanin synthase-like [Glycine max]
          Length = 486

 Score =  200 bits (509), Expect = 2e-49
 Identities = 91/194 (46%), Positives = 134/194 (69%)
 Frame = -1

Query: 584 KIYNRLWVRPKQMEKQLKELGFKGNPYRFLHGDMKDFFSIAAQANSKPINFTNDIRSRVL 405
           ++ N LW+RPK++E+ L+E G +GNPYR L GD+K+   +  +A SKP+N ++DI  RV 
Sbjct: 27  RMLNWLWIRPKRLERLLREQGLQGNPYRILVGDLKEIVKLQMEARSKPMNLSHDIVPRVF 86

Query: 404 PYVHHVVNKHGKSSFVWFGAKAKLNITDPTLVKEILLKFEEFHKVYPDPVADLTVGGLST 225
            ++H  V KHGK+SF+WFG K ++ +TDP L+K++L K  +F K   +P+A L   GL  
Sbjct: 87  AHLHQSVLKHGKNSFIWFGPKPRVTLTDPELIKDVLNKISDFRKPEANPLAKLLATGLVN 146

Query: 224 AHGETWPRHRKILNATFSLEKLKGTLPDIYLSCQDTISKWKALLVSTAGNTEIDAWPYMK 45
             GE W +HR+++N  FSLEKLK  LP  + SC D I KW+ +L S  G+ E+D WP+++
Sbjct: 147 YDGEKWNKHRRLINPAFSLEKLKIMLPIFFKSCNDLIIKWEGML-SYDGSCEMDVWPFLQ 205

Query: 44  NLARDMLSRVAFGS 3
           NLA D+++R AFGS
Sbjct: 206 NLASDVIARTAFGS 219


>ref|XP_003634731.1| PREDICTED: LOW QUALITY PROTEIN: secologanin synthase-like [Vitis
           vinifera]
          Length = 517

 Score =  199 bits (506), Expect = 4e-49
 Identities = 90/194 (46%), Positives = 129/194 (66%)
 Frame = -1

Query: 584 KIYNRLWVRPKQMEKQLKELGFKGNPYRFLHGDMKDFFSIAAQANSKPINFTNDIRSRVL 405
           ++ N +W+RPK++E+ L+E G  GN YR LHGD K+   +  +A S+PI+ ++D   RVL
Sbjct: 22  RLLNWVWLRPKRLERCLREQGLAGNSYRLLHGDFKEMSMMLKEAYSRPISLSDDTTPRVL 81

Query: 404 PYVHHVVNKHGKSSFVWFGAKAKLNITDPTLVKEILLKFEEFHKVYPDPVADLTVGGLST 225
           P+  H + K+GK+SF WFG    +NI +P L++++LLK   F K  P P+  L V GL T
Sbjct: 82  PFHFHFIKKYGKNSFAWFGPNPMVNIMEPELIRDVLLKSNVFQKPPPHPLGKLLVSGLVT 141

Query: 224 AHGETWPRHRKILNATFSLEKLKGTLPDIYLSCQDTISKWKALLVSTAGNTEIDAWPYMK 45
             GE W + RKI+N  F LEKLK  LP  +LSC D ++KWK  ++S  G+ E+D WPY++
Sbjct: 142 LEGERWAKRRKIINPAFHLEKLKNMLPAFHLSCSDMVTKWK--MLSVGGSCELDVWPYLE 199

Query: 44  NLARDMLSRVAFGS 3
           NL  D++SR AFGS
Sbjct: 200 NLTGDVISRTAFGS 213