BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00017589
         (1163 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AGF25266.1| ent-kaurenoic acid oxidase [Pyrus communis]            486   e-135
dbj|BAG71199.1| ent-kaurenoic acid oxidase [Lactuca sativa]           471   e-130
gb|AEF32085.1| ent-kaurenoic acid oxidase [Castanea mollissima]       465   e-128
ref|XP_004168072.1| PREDICTED: LOW QUALITY PROTEIN: ent-kaurenoi...   463   e-128
ref|XP_004153984.1| PREDICTED: ent-kaurenoic acid oxidase 1-like...   463   e-128

>gb|AGF25266.1| ent-kaurenoic acid oxidase [Pyrus communis]
          Length = 503

 Score =  486 bits (1250), Expect = e-135
 Identities = 227/328 (69%), Positives = 276/328 (84%)
 Frame = +1

Query: 1    IEKMVKSGLDKWSKMGQIEFLTELRRLTFRIVMYIFMSSESEQVLEALEKEYTTLNYGVR 180
            IE++V + L+KWSK+GQIEFLT+LR+LTF+I+MYIF+SSESE V+EALE+EYT LNYGVR
Sbjct: 172  IEEIVITSLEKWSKLGQIEFLTQLRKLTFKIIMYIFLSSESEPVMEALEREYTVLNYGVR 231

Query: 181  ALAINVPGFAYHGALKARKKLVAIFQSIVSERRLRXXXXXXXXXXXXXXXALLAVEDENG 360
            A+AIN+PGFAYH ALKARK LVAIFQSIV ERR +               ALL VED++G
Sbjct: 232  AMAINLPGFAYHKALKARKNLVAIFQSIVDERRAQRKSGNYYVKKKDMMDALLDVEDDDG 291

Query: 361  RKLNDEEIIDVLLMYLNAGHESSGHIIMWSTVYLQAHPEIFXXXXXXXXXXXXNRPPNQE 540
            RKLNDE+IIDVLLMYLNAGHESSGH IMW+TV+LQA+PE F             RPP Q+
Sbjct: 292  RKLNDEDIIDVLLMYLNAGHESSGHTIMWATVFLQANPEAFQRAKAEQEEILKRRPPTQK 351

Query: 541  GMTLREYRQMEYLSKVIDEALRLVTFSLTVFREAKKDVKISGYTIPKGWKVLVWFRTVHF 720
            G+TL+EYR+M+YL +VIDE LR++TFSLTVFREAKKDVKI+GY+IPKGWKVLVWFR++H+
Sbjct: 352  GLTLKEYREMDYLCQVIDETLRVITFSLTVFREAKKDVKINGYSIPKGWKVLVWFRSIHY 411

Query: 721  DPEVYPDPQKFDPSRWDDFTPKAGAFLPFGGGTRLCPGNDLAKLEISVFLHHFLLNYELE 900
            D E+YP+P +F+PSRWD+FTPKA +FLPFG G+ LCPGNDLAKLEI++FLHHFLLNY++E
Sbjct: 412  DSELYPNPMEFNPSRWDNFTPKAFSFLPFGAGSHLCPGNDLAKLEIAIFLHHFLLNYKME 471

Query: 901  RDNPECPVMYLPHCRPKDNCLGKVRKVG 984
            R NP CP+MYLPH RPKDNC+ K++K G
Sbjct: 472  RTNPGCPLMYLPHTRPKDNCVAKIKKCG 499


>dbj|BAG71199.1| ent-kaurenoic acid oxidase [Lactuca sativa]
          Length = 493

 Score =  471 bits (1211), Expect = e-130
 Identities = 225/327 (68%), Positives = 262/327 (80%)
 Frame = +1

Query: 1    IEKMVKSGLDKWSKMGQIEFLTELRRLTFRIVMYIFMSSESEQVLEALEKEYTTLNYGVR 180
            IE  V   L+KWSKMG+IEFLT LR+LTF+I+M+IF+S+ESE V++ALEKEYT LNYGVR
Sbjct: 166  IETNVVLALEKWSKMGRIEFLTYLRKLTFQIIMHIFLSTESEHVMDALEKEYTVLNYGVR 225

Query: 181  ALAINVPGFAYHGALKARKKLVAIFQSIVSERRLRXXXXXXXXXXXXXXXALLAVEDENG 360
            A+AIN+PGFAY+ ALKARK LVAI Q++V ERR +               ALL   DE G
Sbjct: 226  AMAINIPGFAYYNALKARKNLVAILQTVVYERR-KKREANEGMSKKDMLDALLDTTDEKG 284

Query: 361  RKLNDEEIIDVLLMYLNAGHESSGHIIMWSTVYLQAHPEIFXXXXXXXXXXXXNRPPNQE 540
            RKL+DEEIID L+MYLNAGHESSGHI MWST+ LQAHPE F            N PP QE
Sbjct: 285  RKLDDEEIIDTLVMYLNAGHESSGHITMWSTILLQAHPEFFHIAKEEQERIVKNMPPTQE 344

Query: 541  GMTLREYRQMEYLSKVIDEALRLVTFSLTVFREAKKDVKISGYTIPKGWKVLVWFRTVHF 720
            G+TL+EYRQMEYLSKVIDE LRLVTFSL  FREAKKDV I GY IPKGWKVL+WFR+VH 
Sbjct: 345  GLTLKEYRQMEYLSKVIDETLRLVTFSLMTFREAKKDVDIKGYFIPKGWKVLLWFRSVHH 404

Query: 721  DPEVYPDPQKFDPSRWDDFTPKAGAFLPFGGGTRLCPGNDLAKLEISVFLHHFLLNYELE 900
            +PE+YP P++F+PSRWDD  PK G FLPFG G+RLCPGNDLAKLEI++FLHHFL+NYE E
Sbjct: 405  NPELYPQPKEFNPSRWDDLVPKPGTFLPFGAGSRLCPGNDLAKLEIAIFLHHFLVNYEFE 464

Query: 901  RDNPECPVMYLPHCRPKDNCLGKVRKV 981
            R+NPECP+MYLPH RPKDNC+G+VR+V
Sbjct: 465  RENPECPIMYLPHSRPKDNCVGRVRRV 491


>gb|AEF32085.1| ent-kaurenoic acid oxidase [Castanea mollissima]
          Length = 492

 Score =  465 bits (1196), Expect = e-128
 Identities = 223/326 (68%), Positives = 262/326 (80%)
 Frame = +1

Query: 1    IEKMVKSGLDKWSKMGQIEFLTELRRLTFRIVMYIFMSSESEQVLEALEKEYTTLNYGVR 180
            IE++V S LDKW+ MG+IEFLTELR+LTFRI+MYIF+SSESE V+ ALE+EYT LNYGVR
Sbjct: 162  IEEIVTSSLDKWTTMGEIEFLTELRKLTFRIIMYIFLSSESEPVMTALEREYTALNYGVR 221

Query: 181  ALAINVPGFAYHGALKARKKLVAIFQSIVSERRLRXXXXXXXXXXXXXXXALLAVEDENG 360
            A+AIN+PGFAYH ALKARK LVA FQSIV +RR                 AL+ V+DENG
Sbjct: 222  AMAINIPGFAYHKALKARKNLVATFQSIVDDRR-NQKKVSVPTKKKDMMDALIDVKDENG 280

Query: 361  RKLNDEEIIDVLLMYLNAGHESSGHIIMWSTVYLQAHPEIFXXXXXXXXXXXXNRPPNQE 540
            RKL DEEIID+L+MYLNAGHESSGH  MW+T++L  HPE               R P Q+
Sbjct: 281  RKLTDEEIIDILVMYLNAGHESSGHTAMWATIFLNQHPEYLQKAKKEQEEIVRKRLPEQK 340

Query: 541  GMTLREYRQMEYLSKVIDEALRLVTFSLTVFREAKKDVKISGYTIPKGWKVLVWFRTVHF 720
            G+TL+E R+MEYLSKVIDE LR++TFSLTVFREAK+DV I+GYTIPKGW+VLVWFR+VH 
Sbjct: 341  GLTLKEIREMEYLSKVIDETLRVITFSLTVFREAKQDVNIAGYTIPKGWRVLVWFRSVHL 400

Query: 721  DPEVYPDPQKFDPSRWDDFTPKAGAFLPFGGGTRLCPGNDLAKLEISVFLHHFLLNYELE 900
            DPE+Y +P++F PSRWD+FTPKAGAFLPFG GTRLCPGNDLAKLEIS+FLHHFLLNY+LE
Sbjct: 401  DPEIYENPKEFKPSRWDNFTPKAGAFLPFGAGTRLCPGNDLAKLEISIFLHHFLLNYQLE 460

Query: 901  RDNPECPVMYLPHCRPKDNCLGKVRK 978
            R NP  P MYLPH RP DNCL +++K
Sbjct: 461  RLNPGSPSMYLPHTRPVDNCLARIKK 486


>ref|XP_004168072.1| PREDICTED: LOW QUALITY PROTEIN: ent-kaurenoic acid oxidase 1-like
            [Cucumis sativus]
          Length = 484

 Score =  463 bits (1191), Expect = e-128
 Identities = 223/328 (67%), Positives = 264/328 (80%), Gaps = 1/328 (0%)
 Frame = +1

Query: 1    IEKMVKSGLDKWSKMGQIEFLTELRRLTFRIVMYIFMSSESEQVLEALEKEYTTLNYGVR 180
            IEK+V S LDKW  MG+IEFLTELR+LTF+I+MYIF+SSESE ++EALE+EYT LN GVR
Sbjct: 156  IEKIVVSSLDKWGNMGEIEFLTELRKLTFKIIMYIFLSSESETIMEALEREYTMLNLGVR 215

Query: 181  ALAINVPGFAYHGALKARKKLVAIFQSIVSERRL-RXXXXXXXXXXXXXXXALLAVEDEN 357
            A+AIN+PGFAY+ ALKARK L A F SIV  RR+ R               ALL  EDE+
Sbjct: 216  AMAINIPGFAYYKALKARKNLEATFGSIVRGRRMERERKENSKTMKRDMLDALLEAEDED 275

Query: 358  GRKLNDEEIIDVLLMYLNAGHESSGHIIMWSTVYLQAHPEIFXXXXXXXXXXXXNRPPNQ 537
            GR+L+DEEIIDVL+MYLNAGHESSGH +MW+T++LQ HP                RP  Q
Sbjct: 276  GRRLSDEEIIDVLIMYLNAGHESSGHTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQ 335

Query: 538  EGMTLREYRQMEYLSKVIDEALRLVTFSLTVFREAKKDVKISGYTIPKGWKVLVWFRTVH 717
            +G+TL+E RQM YLSKVIDE LR+VTFSLTVFREAK DVKISGYTIPKGWKVLVWFR+VH
Sbjct: 336  KGLTLKEVRQMPYLSKVIDETLRVVTFSLTVFREAKHDVKISGYTIPKGWKVLVWFRSVH 395

Query: 718  FDPEVYPDPQKFDPSRWDDFTPKAGAFLPFGGGTRLCPGNDLAKLEISVFLHHFLLNYEL 897
            FD E YPDP++F+PSRWDDF PKAG+FLPFG G+RLCPGNDLAKLEISVFLH+FLLNY+L
Sbjct: 396  FDSETYPDPREFNPSRWDDFIPKAGSFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKL 455

Query: 898  ERDNPECPVMYLPHCRPKDNCLGKVRKV 981
            ER NPE P+ YLPH +P+DNCLG+++++
Sbjct: 456  ERVNPESPIRYLPHSKPEDNCLGRIKRL 483


>ref|XP_004153984.1| PREDICTED: ent-kaurenoic acid oxidase 1-like [Cucumis sativus]
          Length = 407

 Score =  463 bits (1191), Expect = e-128
 Identities = 223/328 (67%), Positives = 264/328 (80%), Gaps = 1/328 (0%)
 Frame = +1

Query: 1    IEKMVKSGLDKWSKMGQIEFLTELRRLTFRIVMYIFMSSESEQVLEALEKEYTTLNYGVR 180
            IEK+V S LDKW  MG+IEFLTELR+LTF+I+MYIF+SSESE ++EALE+EYT LN GVR
Sbjct: 79   IEKIVVSSLDKWGNMGEIEFLTELRKLTFKIIMYIFLSSESETIMEALEREYTMLNLGVR 138

Query: 181  ALAINVPGFAYHGALKARKKLVAIFQSIVSERRL-RXXXXXXXXXXXXXXXALLAVEDEN 357
            A+AIN+PGFAY+ ALKARK L A F SIV  RR+ R               ALL  EDE+
Sbjct: 139  AMAINIPGFAYYKALKARKNLEATFGSIVRGRRMERERKENSKTMKRDMLDALLEAEDED 198

Query: 358  GRKLNDEEIIDVLLMYLNAGHESSGHIIMWSTVYLQAHPEIFXXXXXXXXXXXXNRPPNQ 537
            GR+L+DEEIIDVL+MYLNAGHESSGH +MW+T++LQ HP                RP  Q
Sbjct: 199  GRRLSDEEIIDVLIMYLNAGHESSGHTMMWATIFLQQHPHFLQKAKAEQEEMIKKRPVGQ 258

Query: 538  EGMTLREYRQMEYLSKVIDEALRLVTFSLTVFREAKKDVKISGYTIPKGWKVLVWFRTVH 717
            +G+TL+E RQM YLSKVIDE LR+VTFSLTVFREAK DVKISGYTIPKGWKVLVWFR+VH
Sbjct: 259  KGLTLKEVRQMPYLSKVIDETLRVVTFSLTVFREAKHDVKISGYTIPKGWKVLVWFRSVH 318

Query: 718  FDPEVYPDPQKFDPSRWDDFTPKAGAFLPFGGGTRLCPGNDLAKLEISVFLHHFLLNYEL 897
            FD E YPDP++F+PSRWDDF PKAG+FLPFG G+RLCPGNDLAKLEISVFLH+FLLNY+L
Sbjct: 319  FDSETYPDPREFNPSRWDDFIPKAGSFLPFGAGSRLCPGNDLAKLEISVFLHYFLLNYKL 378

Query: 898  ERDNPECPVMYLPHCRPKDNCLGKVRKV 981
            ER NPE P+ YLPH +P+DNCLG+++++
Sbjct: 379  ERVNPESPIRYLPHSKPEDNCLGRIKRL 406