BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00017477
         (1474 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFD98849.1| DNA polymerase alpha catalytic subunit [Solanum l...   672   0.0  
ref|XP_002523609.1| DNA polymerase alpha catalytic subunit, puta...   665   0.0  
ref|XP_002277947.2| PREDICTED: DNA polymerase alpha catalytic su...   662   0.0  
ref|XP_002310719.1| predicted protein [Populus trichocarpa] gi|2...   659   0.0  
emb|CBH32588.1| DNA polymerase alpha catalytic subunit,putative,...   605   0.0  

>gb|AFD98849.1| DNA polymerase alpha catalytic subunit [Solanum lycopersicum]
          Length = 1561

 Score =  672 bits (1734), Expect = 0.0
 Identities = 331/394 (84%), Positives = 357/394 (90%)
 Frame = +2

Query: 293  KSKKGPGYAGGLVLEPKKGLYDKYILLLDFNSLYPSIIQEYNICFTTVERPVDGSIPRLP 472
            K+KKGP Y+GGLVLEPK+GLYDKYILLLDFNSLYPSIIQEYNICFTTVE  +DGS+P LP
Sbjct: 926  KAKKGPSYSGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVESSLDGSVPHLP 985

Query: 473  SSRRTGVLPELLKNLVERRRMVKSWLKTASGLKVQQLDIQQQALKLTANSMYGCLGFASS 652
            SS+RTG+LPELLKNLVERRRMVKSWLKTASGLK QQ DIQQQALKLTANSMYGCLGF++S
Sbjct: 986  SSKRTGLLPELLKNLVERRRMVKSWLKTASGLKAQQFDIQQQALKLTANSMYGCLGFSNS 1045

Query: 653  RFYAKPLAELITLQGREILQSTVDLVQNNLNLEVIYGDTDSIMIYSGLDDVAKAKSISGK 832
            RFYAK LAELIT QGREILQSTVDLVQN LNLEVIYGDTDSIMIYSGLDD+ K+K+I+ K
Sbjct: 1046 RFYAKSLAELITSQGREILQSTVDLVQNLLNLEVIYGDTDSIMIYSGLDDIGKSKAIAAK 1105

Query: 833  VIQEVNKKYKCLEIDLDGXXXXXXXXXXXXXXXXXXQIKDGKPYEVIERKGLDMVRRDWS 1012
            VIQEVNKKY+CLEIDLDG                  Q KDGKPYEVIE+KGLDMVRRDWS
Sbjct: 1106 VIQEVNKKYRCLEIDLDGLYKRMLLLKKKKYAAVKVQFKDGKPYEVIEKKGLDMVRRDWS 1165

Query: 1013 LLAKELGDYCLSQILSGGSCEDVVESIHDALMKVQEDMRNGRIALEKYVITKSLTRAPEA 1192
            LL+KELGD+CLSQILSGGSCEDV+ESIH+ALMKVQE MR G+I LEKYVITKSLT+ PEA
Sbjct: 1166 LLSKELGDFCLSQILSGGSCEDVIESIHNALMKVQEQMRTGQIELEKYVITKSLTKPPEA 1225

Query: 1193 YPDARNQPHVEVALRLKKNGYVTGCSAGDTVPYVICCEQGNSLTTSVGIAQRARHPDELK 1372
            YPDA++QPHVEVALRLKK+GYVTGCSAGDTVPYVICCEQGN  TTSVGIAQRARHPDELK
Sbjct: 1226 YPDAKSQPHVEVALRLKKSGYVTGCSAGDTVPYVICCEQGNGSTTSVGIAQRARHPDELK 1285

Query: 1373 TDNGNWMVDIDYYLAQQIHPVVSRLCASIQGTSP 1474
             DNGNW+VDIDYYLAQQIHPV+SRLCASIQGTSP
Sbjct: 1286 RDNGNWIVDIDYYLAQQIHPVISRLCASIQGTSP 1319



 Score = 87.4 bits (215), Expect(2) = 3e-22
 Identities = 53/116 (45%), Positives = 68/116 (58%)
 Frame = +3

Query: 72   GKTLQGARAQRVEYLLLHAFHAKKFIVPDKQSFQMKDTKAPKRKTKNGVEENEIQNLNPA 251
            GKTLQGARAQRVEYLLLHAFHAKKFIVPDK S   ++ K  KRK   G E  E + ++  
Sbjct: 852  GKTLQGARAQRVEYLLLHAFHAKKFIVPDKFSSHAREAKITKRKLNQGDEGKETEPIDAD 911

Query: 252  DTNIDIESPQMINGSPKRGPAMLVV*FWNRKRVFMTSTYYFWILIVSTHRLYRNTI 419
            D NI+     + +G  K+GP+         KR      Y  +IL++  + LY + I
Sbjct: 912  DPNIEGGILDINHGKAKKGPSYSGGLVLEPKR----GLYDKYILLLDFNSLYPSII 963



 Score = 45.4 bits (106), Expect(2) = 3e-22
 Identities = 23/38 (60%), Positives = 26/38 (68%), Gaps = 9/38 (23%)
 Frame = +1

Query: 1   TDAWLSMELMFH---------LTNISGNLWGNRVKLSR 87
           TDAWLSMELMFH         LTNISGNLWG  ++ +R
Sbjct: 822 TDAWLSMELMFHLSVLPLTRQLTNISGNLWGKTLQGAR 859


>ref|XP_002523609.1| DNA polymerase alpha catalytic subunit, putative [Ricinus communis]
            gi|223537171|gb|EEF38804.1| DNA polymerase alpha
            catalytic subunit, putative [Ricinus communis]
          Length = 1526

 Score =  665 bits (1717), Expect = 0.0
 Identities = 324/398 (81%), Positives = 357/398 (89%)
 Frame = +2

Query: 281  NDKWKSKKGPGYAGGLVLEPKKGLYDKYILLLDFNSLYPSIIQEYNICFTTVERPVDGSI 460
            ND+ K KKGP Y GGLVLEPKKGLYDKY+LLLDFNSLYPSIIQEYNICFTTVE+  DG +
Sbjct: 886  NDRGKGKKGPAYVGGLVLEPKKGLYDKYVLLLDFNSLYPSIIQEYNICFTTVEKSTDGLV 945

Query: 461  PRLPSSRRTGVLPELLKNLVERRRMVKSWLKTASGLKVQQLDIQQQALKLTANSMYGCLG 640
            PRLPS + TGVLPELLKNLVERRRMVKSW+K ASGLKVQQLDIQQQALKLTANSMYGCLG
Sbjct: 946  PRLPSIKTTGVLPELLKNLVERRRMVKSWMKNASGLKVQQLDIQQQALKLTANSMYGCLG 1005

Query: 641  FASSRFYAKPLAELITLQGREILQSTVDLVQNNLNLEVIYGDTDSIMIYSGLDDVAKAKS 820
            F++SRFYAKPLAELITLQGREILQSTVDLVQNNLNLEVIYGDTDSIMIYSGL+D+ KAK+
Sbjct: 1006 FSNSRFYAKPLAELITLQGREILQSTVDLVQNNLNLEVIYGDTDSIMIYSGLEDITKAKA 1065

Query: 821  ISGKVIQEVNKKYKCLEIDLDGXXXXXXXXXXXXXXXXXXQIKDGKPYEVIERKGLDMVR 1000
            I+GKVIQEVNKKY+CLEIDLDG                  Q KDG PYEVIERKGLDMVR
Sbjct: 1066 IAGKVIQEVNKKYRCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGTPYEVIERKGLDMVR 1125

Query: 1001 RDWSLLAKELGDYCLSQILSGGSCEDVVESIHDALMKVQEDMRNGRIALEKYVITKSLTR 1180
            RDWSLL+KELGD+CL+QILSGGSCEDVVESIH++LMKVQE+MRNG++ALEKY+ITK+LT+
Sbjct: 1126 RDWSLLSKELGDFCLAQILSGGSCEDVVESIHNSLMKVQEEMRNGQVALEKYIITKTLTK 1185

Query: 1181 APEAYPDARNQPHVEVALRLKKNGYVTGCSAGDTVPYVICCEQGNSLTTSVGIAQRARHP 1360
             PEAYPDA+NQPHV VALRLK++GY TGCS GDTVPY+ICCEQG +  +S GIAQRARHP
Sbjct: 1186 PPEAYPDAKNQPHVMVALRLKQSGYTTGCSVGDTVPYIICCEQGATPGSSSGIAQRARHP 1245

Query: 1361 DELKTDNGNWMVDIDYYLAQQIHPVVSRLCASIQGTSP 1474
            DELK D+G WM+DIDYYL+QQIHPVVSRLCASIQGTSP
Sbjct: 1246 DELKKDDGKWMIDIDYYLSQQIHPVVSRLCASIQGTSP 1283



 Score =  101 bits (251), Expect(2) = 2e-26
 Identities = 51/82 (62%), Positives = 60/82 (73%), Gaps = 1/82 (1%)
 Frame = +3

Query: 72   GKTLQGARAQRVEYLLLHAFHAKKFIVPDKQSFQM-KDTKAPKRKTKNGVEENEIQNLNP 248
            GKTLQGARAQRVEYLLLHAFHAKK+IVPDK SF + K+TK  KR+  NGVEE   + L+ 
Sbjct: 815  GKTLQGARAQRVEYLLLHAFHAKKYIVPDKNSFHLKKETKMTKRRIHNGVEEKNAEELDT 874

Query: 249  ADTNIDIESPQMINGSPKRGPA 314
               N D +SP+   G  K+GPA
Sbjct: 875  DHANFDNDSPENDRGKGKKGPA 896



 Score = 45.4 bits (106), Expect(2) = 2e-26
 Identities = 23/38 (60%), Positives = 26/38 (68%), Gaps = 9/38 (23%)
 Frame = +1

Query: 1   TDAWLSMELMFH---------LTNISGNLWGNRVKLSR 87
           TDAWLSMELMFH         LTNISGNLWG  ++ +R
Sbjct: 785 TDAWLSMELMFHLSVLPLTRQLTNISGNLWGKTLQGAR 822


>ref|XP_002277947.2| PREDICTED: DNA polymerase alpha catalytic subunit-like [Vitis
            vinifera]
          Length = 1522

 Score =  662 bits (1707), Expect = 0.0
 Identities = 329/398 (82%), Positives = 357/398 (89%)
 Frame = +2

Query: 281  NDKWKSKKGPGYAGGLVLEPKKGLYDKYILLLDFNSLYPSIIQEYNICFTTVERPVDGSI 460
            N++ K KKGP Y+GGLVLEPK+GLYDKYILLLDFNSLYPSIIQEYNICFTTVER  DGS+
Sbjct: 886  NNQGKGKKGPAYSGGLVLEPKRGLYDKYILLLDFNSLYPSIIQEYNICFTTVERSPDGSV 945

Query: 461  PRLPSSRRTGVLPELLKNLVERRRMVKSWLKTASGLKVQQLDIQQQALKLTANSMYGCLG 640
            PRLPSS+ TGVLPELLK LVERR+ VKS LKTASGLKVQQLDIQQQALKLTANSMYGCLG
Sbjct: 946  PRLPSSKTTGVLPELLKKLVERRKTVKSRLKTASGLKVQQLDIQQQALKLTANSMYGCLG 1005

Query: 641  FASSRFYAKPLAELITLQGREILQSTVDLVQNNLNLEVIYGDTDSIMIYSGLDDVAKAKS 820
            F++SRFYAKPLAELITLQGREILQSTVDLVQNNLNLEVIYGDTDSIMIY+GLDD+ KAK+
Sbjct: 1006 FSNSRFYAKPLAELITLQGREILQSTVDLVQNNLNLEVIYGDTDSIMIYTGLDDITKAKA 1065

Query: 821  ISGKVIQEVNKKYKCLEIDLDGXXXXXXXXXXXXXXXXXXQIKDGKPYEVIERKGLDMVR 1000
            I+GKVIQEVNKKY+CLEIDLDG                  Q KDG PYEVIERKGLDMVR
Sbjct: 1066 IAGKVIQEVNKKYRCLEIDLDGLYKRMLLLKKKKYAAVKLQFKDGTPYEVIERKGLDMVR 1125

Query: 1001 RDWSLLAKELGDYCLSQILSGGSCEDVVESIHDALMKVQEDMRNGRIALEKYVITKSLTR 1180
            RDWSLL+KELGD+ LSQILSGGSCEDVVESIH++LMKVQEDMRNG +ALEKY+ITKSLT+
Sbjct: 1126 RDWSLLSKELGDFSLSQILSGGSCEDVVESIHNSLMKVQEDMRNGEVALEKYIITKSLTK 1185

Query: 1181 APEAYPDARNQPHVEVALRLKKNGYVTGCSAGDTVPYVICCEQGNSLTTSVGIAQRARHP 1360
             PEAYPD +NQPHV+VALRLK++GY TGCSAGDTVPY+ICCEQG S  +S GIAQRARHP
Sbjct: 1186 PPEAYPD-KNQPHVQVALRLKQSGYSTGCSAGDTVPYIICCEQGTSSGSSTGIAQRARHP 1244

Query: 1361 DELKTDNGNWMVDIDYYLAQQIHPVVSRLCASIQGTSP 1474
            DELK DNG WM+DIDYYLAQQIHPVVSRLCASIQGTSP
Sbjct: 1245 DELKRDNGKWMIDIDYYLAQQIHPVVSRLCASIQGTSP 1282



 Score = 85.5 bits (210), Expect(2) = 1e-21
 Identities = 51/117 (43%), Positives = 70/117 (59%), Gaps = 1/117 (0%)
 Frame = +3

Query: 72   GKTLQGARAQRVEYLLLHAFHAKKFIVPDKQSFQMKDTKAPKRK-TKNGVEENEIQNLNP 248
            GKTLQGARAQRVEYLLLH FHAKK+IVPDK S  +++TK  KR+  + G E+  I  L+ 
Sbjct: 815  GKTLQGARAQRVEYLLLHEFHAKKYIVPDKISSHLRETKWTKRRMNQGGAEDRNIDELDV 874

Query: 249  ADTNIDIESPQMINGSPKRGPAMLVV*FWNRKRVFMTSTYYFWILIVSTHRLYRNTI 419
             D +++ ++     G  K+GPA         KR      Y  +IL++  + LY + I
Sbjct: 875  DDAHVENDAHHNNQGKGKKGPAYSGGLVLEPKR----GLYDKYILLLDFNSLYPSII 927



 Score = 45.4 bits (106), Expect(2) = 1e-21
 Identities = 23/38 (60%), Positives = 26/38 (68%), Gaps = 9/38 (23%)
 Frame = +1

Query: 1   TDAWLSMELMFH---------LTNISGNLWGNRVKLSR 87
           TDAWLSMELMFH         LTNISGNLWG  ++ +R
Sbjct: 785 TDAWLSMELMFHLSVLPLTRLLTNISGNLWGKTLQGAR 822


>ref|XP_002310719.1| predicted protein [Populus trichocarpa] gi|222853622|gb|EEE91169.1|
            predicted protein [Populus trichocarpa]
          Length = 1312

 Score =  659 bits (1700), Expect = 0.0
 Identities = 324/400 (81%), Positives = 357/400 (89%), Gaps = 2/400 (0%)
 Frame = +2

Query: 281  NDKWKSKKGPGYAGGLVLEPKKGLYDKYILLLDFNSLYPSIIQEYNICFTTVERPVDGSI 460
            +D  K KKGP YAGGLVLEPKKGLYDKY+LLLDFNSLYPSIIQEYNICFTTVER +DG +
Sbjct: 671  SDHGKRKKGPAYAGGLVLEPKKGLYDKYVLLLDFNSLYPSIIQEYNICFTTVERSMDGLV 730

Query: 461  PRLPSSRRTGVLPELLKNLVERRRMVKSWLKTASGLKVQQLDIQQQALKLTANSMYGCLG 640
            PRLPSS+ TGVLPELLKNLVERRRMVKSW+K ASGLKVQQLDIQQQALKLTANSMYGCLG
Sbjct: 731  PRLPSSKTTGVLPELLKNLVERRRMVKSWMKNASGLKVQQLDIQQQALKLTANSMYGCLG 790

Query: 641  FASSRFYAKPLAELITLQGREILQSTVDLVQNNLNLEVIYGDTDSIMIYSGLDDVAKAKS 820
            F++SRFYAKPLAELITLQGREILQSTVDLVQNNLNLEVIYGDTDSIMIYSGLDD+ KAK+
Sbjct: 791  FSNSRFYAKPLAELITLQGREILQSTVDLVQNNLNLEVIYGDTDSIMIYSGLDDIPKAKA 850

Query: 821  ISGKVIQEVNKKYKCLEIDLDGXXXXXXXXXXXXXXXXXXQIKDGKPYEVIERKGLDMVR 1000
            I+GKVIQEVNKKY+CLEIDLDG                  Q KDG PYEVIERKGLDMVR
Sbjct: 851  IAGKVIQEVNKKYRCLEIDLDGLYKRMLLLKKKKYAAVKVQFKDGTPYEVIERKGLDMVR 910

Query: 1001 RDWSLLAKELGDYCLSQILSGGSCEDVVESIHDALMKVQEDMRNGRIALEKYVITKSLTR 1180
            RDWSLL+KELGD+CL+QILSGGSCEDV+ESIH++LMKVQEDMR+G++ALEKYVITK+LT+
Sbjct: 911  RDWSLLSKELGDFCLAQILSGGSCEDVIESIHNSLMKVQEDMRSGQVALEKYVITKTLTK 970

Query: 1181 APEAYPDARNQPHVEVALRLKKNGYVTGCSAGDTVPYVICCEQ--GNSLTTSVGIAQRAR 1354
             PEAYPDA+NQPH  VALRLK++GY  GCSAGDTVPY+ICCEQ  G S  +  GIAQRAR
Sbjct: 971  PPEAYPDAKNQPHALVALRLKQSGYTAGCSAGDTVPYIICCEQGAGASAVSLTGIAQRAR 1030

Query: 1355 HPDELKTDNGNWMVDIDYYLAQQIHPVVSRLCASIQGTSP 1474
            HPDELK D+G W++DI+YYL+QQIHPVVSRLCASIQGTSP
Sbjct: 1031 HPDELKCDDGKWIIDIEYYLSQQIHPVVSRLCASIQGTSP 1070



 Score = 94.7 bits (234), Expect(2) = 2e-24
 Identities = 45/81 (55%), Positives = 58/81 (71%)
 Frame = +3

Query: 72  GKTLQGARAQRVEYLLLHAFHAKKFIVPDKQSFQMKDTKAPKRKTKNGVEENEIQNLNPA 251
           GKTLQGARAQRVEYLLLHAFHAKK+IVPDK S ++K+ K  KR+  NG+E+     L+  
Sbjct: 601 GKTLQGARAQRVEYLLLHAFHAKKYIVPDKMSSRVKEAKITKRRINNGIEDRNADELDTD 660

Query: 252 DTNIDIESPQMINGSPKRGPA 314
             N + ++ Q  +G  K+GPA
Sbjct: 661 AANFENDNQQSDHGKRKKGPA 681



 Score = 45.4 bits (106), Expect(2) = 2e-24
 Identities = 23/38 (60%), Positives = 26/38 (68%), Gaps = 9/38 (23%)
 Frame = +1

Query: 1   TDAWLSMELMFH---------LTNISGNLWGNRVKLSR 87
           TDAWLSMELMFH         LTNISGNLWG  ++ +R
Sbjct: 571 TDAWLSMELMFHLSILPLTRQLTNISGNLWGKTLQGAR 608


>emb|CBH32588.1| DNA polymerase alpha catalytic subunit,putative,expressed [Triticum
            aestivum]
          Length = 1369

 Score =  605 bits (1560), Expect(3) = 0.0
 Identities = 303/401 (75%), Positives = 346/401 (86%)
 Frame = +2

Query: 272  VSTNDKWKSKKGPGYAGGLVLEPKKGLYDKYILLLDFNSLYPSIIQEYNICFTTVERPVD 451
            V   D  K +K P YAGGLVLEPKKGLYDKYILLLDFNSLYPSIIQE+NICFTTVER  D
Sbjct: 749  VHHGDPGKVRKVPSYAGGLVLEPKKGLYDKYILLLDFNSLYPSIIQEFNICFTTVERSSD 808

Query: 452  GSIPRLPSSRRTGVLPELLKNLVERRRMVKSWLKTASGLKVQQLDIQQQALKLTANSMYG 631
            G++P LP+S+ TGVLPELLK+LVERRRMVKSWLKTASGLK QQ DIQQQALKLTANSMYG
Sbjct: 809  GNLPNLPTSKATGVLPELLKSLVERRRMVKSWLKTASGLKRQQFDIQQQALKLTANSMYG 868

Query: 632  CLGFASSRFYAKPLAELITLQGREILQSTVDLVQNNLNLEVIYGDTDSIMIYSGLDDVAK 811
            CLGF SSRF+AKPLAELITLQGREILQ+TV+LVQNNLNLEVIYGDTDSIMIY+GLDD++K
Sbjct: 869  CLGFTSSRFFAKPLAELITLQGREILQNTVNLVQNNLNLEVIYGDTDSIMIYTGLDDISK 928

Query: 812  AKSISGKVIQEVNKKYKCLEIDLDGXXXXXXXXXXXXXXXXXXQIKDGKPYEVIERKGLD 991
            AKSI+GKVIQEVNKKY+CLEIDLDG                   + DG   E IERKGLD
Sbjct: 929  AKSIAGKVIQEVNKKYRCLEIDLDGVYKRMLLLKKKKYAAIKVAL-DGSLRENIERKGLD 987

Query: 992  MVRRDWSLLAKELGDYCLSQILSGGSCEDVVESIHDALMKVQEDMRNGRIALEKYVITKS 1171
            MVRRDWSLL+KE+GD+CL+QILSGG+C+DVVESIH++L++VQ  M++G+I LEKY+ITKS
Sbjct: 988  MVRRDWSLLSKEIGDFCLNQILSGGTCDDVVESIHNSLVQVQAQMKSGQIELEKYIITKS 1047

Query: 1172 LTRAPEAYPDARNQPHVEVALRLKKNGYVTGCSAGDTVPYVICCEQGNSLTTSVGIAQRA 1351
            LT+APE YPDA+NQPHV+VALRLK+NG+ +GCSAGDTVPY+IC +Q +  T S GIAQRA
Sbjct: 1048 LTKAPEDYPDAKNQPHVQVALRLKQNGF-SGCSAGDTVPYIICSQQDSDKTHSGGIAQRA 1106

Query: 1352 RHPDELKTDNGNWMVDIDYYLAQQIHPVVSRLCASIQGTSP 1474
            RHPDEL  D   WM+DI+YYL+QQIHPVVSRLCASI+GTSP
Sbjct: 1107 RHPDELTRDPNKWMIDIEYYLSQQIHPVVSRLCASIEGTSP 1147



 Score = 58.5 bits (140), Expect(3) = 0.0
 Identities = 31/44 (70%), Positives = 35/44 (79%)
 Frame = +3

Query: 72  GKTLQGARAQRVEYLLLHAFHAKKFIVPDKQSFQMKDTKAPKRK 203
           GKTLQGARAQRVEYLLLH+F +KKFIVPDK +   K+    KRK
Sbjct: 688 GKTLQGARAQRVEYLLLHSFPSKKFIVPDKFA-ACKELNFAKRK 730



 Score = 39.3 bits (90), Expect(3) = 0.0
 Identities = 21/38 (55%), Positives = 25/38 (65%), Gaps = 9/38 (23%)
 Frame = +1

Query: 1   TDAWLSMELMFHL---------TNISGNLWGNRVKLSR 87
           TDA LS+ELMFHL         TNISGNLWG  ++ +R
Sbjct: 658 TDACLSLELMFHLSVLPLTRQLTNISGNLWGKTLQGAR 695