BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00017475
         (1339 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003554768.1| PREDICTED: aspartic proteinase nepenthesin-1...   319   1e-84
ref|XP_003520873.1| PREDICTED: aspartic proteinase nepenthesin-1...   311   2e-82
ref|XP_002523337.1| Aspartic proteinase nepenthesin-1 precursor,...   310   4e-82
ref|XP_002302634.1| predicted protein [Populus trichocarpa] gi|2...   310   5e-82
ref|XP_002263964.1| PREDICTED: aspartic proteinase nepenthesin-1...   307   4e-81

>ref|XP_003554768.1| PREDICTED: aspartic proteinase nepenthesin-1-like [Glycine max]
          Length = 472

 Score =  319 bits (817), Expect = 1e-84
 Identities = 162/256 (63%), Positives = 189/256 (73%), Gaps = 1/256 (0%)
 Frame = -3

Query: 767 EQDTLSLQLHHLDHLNLGDANATGDALFKSRLSRDAARVEALHAISAVKTNSTTTRRRGK 588
           E++ LSL LHH+D L+   +N T + LF+ RL RDA RVE + A++A+  N +  RR G 
Sbjct: 58  EEEALSLHLHHIDALS---SNKTPEQLFQLRLQRDAKRVEGVVALAAL--NQSHARRSGS 112

Query: 587 XXXXXXXXXXSGLAHGSGEYFTRLGVGTPARYSYMVLDTGSDVVWIQCSPCRNCYTQSDP 408
                      GLA GSGEYFTR+GVGTPARY YMVLDTGSDVVW+QC+PCR CYTQ+DP
Sbjct: 113 SFSSSIIS---GLAQGSGEYFTRIGVGTPARYVYMVLDTGSDVVWLQCAPCRKCYTQADP 169

Query: 407 VFDPTKSSTFAGVTCASPLCKRLDSPGC-NKGKKCLYQVSYGDGSFTVGEFSTETMTFRR 231
           VFDPTKS T+AG+ C +PLC+RLDSPGC NK K C YQVSYGDGSFT G+FSTET+TFRR
Sbjct: 170 VFDPTKSRTYAGIPCGAPLCRRLDSPGCNNKNKVCQYQVSYGDGSFTFGDFSTETLTFRR 229

Query: 230 NRVKNIALGCGHDNEGLFVXXXXXXXXXXXXLSFPSQAGPRFGGAFSYCLVDRSASSKPS 51
            RV  +ALGCGHDNEGLF+            LSFP Q G RF   FSYCLVDRSAS+KPS
Sbjct: 230 TRVTRVALGCGHDNEGLFIGAAGLLGLGRGRLSFPVQTGRRFNQKFSYCLVDRSASAKPS 289

Query: 50  SLIFGSSAVSRKAVFT 3
           S++FG SAVSR A FT
Sbjct: 290 SVVFGDSAVSRTARFT 305


>ref|XP_003520873.1| PREDICTED: aspartic proteinase nepenthesin-1-like [Glycine max]
          Length = 461

 Score =  311 bits (798), Expect = 2e-82
 Identities = 167/264 (63%), Positives = 186/264 (70%), Gaps = 4/264 (1%)
 Frame = -3

Query: 782 VDETLE--QDTLSLQLH-HLDHLNLGDANATGDALFKSRLSRDAARVEALHAISAVKTNS 612
           + ETL   Q+TLSL LH HL H++   +N T + LF  RL RDA RVEAL        N 
Sbjct: 40  LSETLSEPQETLSLSLHLHLHHIDALSSNKTPEQLFHLRLQRDAKRVEAL-------LNQ 92

Query: 611 TTTRRRGKXXXXXXXXXXSGLAHGSGEYFTRLGVGTPARYSYMVLDTGSDVVWIQCSPCR 432
              RR             SGLA GSGEYFTR+GVGTPARY YMVLDTGSDVVW+QC+PCR
Sbjct: 93  IHARRSA--GSSFSSSIISGLAQGSGEYFTRIGVGTPARYVYMVLDTGSDVVWLQCAPCR 150

Query: 431 NCYTQSDPVFDPTKSSTFAGVTCASPLCKRLDSPGC-NKGKKCLYQVSYGDGSFTVGEFS 255
            CYTQ+D VFDPTKS T+AG+ C +PLC+RLDSPGC NK K C YQVSYGDGSFT G+FS
Sbjct: 151 KCYTQTDHVFDPTKSRTYAGIPCGAPLCRRLDSPGCSNKNKVCQYQVSYGDGSFTFGDFS 210

Query: 254 TETMTFRRNRVKNIALGCGHDNEGLFVXXXXXXXXXXXXLSFPSQAGPRFGGAFSYCLVD 75
           TET+TFRRNRV  +ALGCGHDNEGLF             LSFP Q G RF   FSYCLVD
Sbjct: 211 TETLTFRRNRVTRVALGCGHDNEGLFTGAAGLLGLGRGRLSFPVQTGRRFNHKFSYCLVD 270

Query: 74  RSASSKPSSLIFGSSAVSRKAVFT 3
           RSAS+KPSS+IFG SAVSR A FT
Sbjct: 271 RSASAKPSSVIFGDSAVSRTAHFT 294


>ref|XP_002523337.1| Aspartic proteinase nepenthesin-1 precursor, putative [Ricinus
           communis] gi|223537425|gb|EEF39053.1| Aspartic
           proteinase nepenthesin-1 precursor, putative [Ricinus
           communis]
          Length = 469

 Score =  310 bits (795), Expect = 4e-82
 Identities = 161/253 (63%), Positives = 183/253 (72%), Gaps = 1/253 (0%)
 Frame = -3

Query: 758 TLSLQLHHLDHLNLGDANATGDALFKSRLSRDAARVEALHAISAVKTNSTTTRRRGKXXX 579
           T S+QLHH+D L+    N+T + LF +RL RDAARVEA   IS +   + T +R G    
Sbjct: 59  TFSVQLHHVDALSF---NSTPETLFTTRLQRDAARVEA---ISYLAETAGTGKRVGTGFS 112

Query: 578 XXXXXXXSGLAHGSGEYFTRLGVGTPARYSYMVLDTGSDVVWIQCSPCRNCYTQSDPVFD 399
                   GLA GSGEYFTR+GVGTP RY YMVLDTGSD+VWIQC+PC+ CY QSDPVFD
Sbjct: 113 SSVIS---GLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWIQCAPCKRCYAQSDPVFD 169

Query: 398 PTKSSTFAGVTCASPLCKRLDSPGCNKGKK-CLYQVSYGDGSFTVGEFSTETMTFRRNRV 222
           P KS +FA + C SPLC RLDSPGCN  K+ C+YQVSYGDGSFT G+FSTET+TFRR RV
Sbjct: 170 PRKSRSFASIACRSPLCHRLDSPGCNTQKQTCMYQVSYGDGSFTFGDFSTETLTFRRTRV 229

Query: 221 KNIALGCGHDNEGLFVXXXXXXXXXXXXLSFPSQAGPRFGGAFSYCLVDRSASSKPSSLI 42
             +ALGCGHDNEGLFV            LSFPSQ G RF   FSYCLVDRSASSKPSS++
Sbjct: 230 ARVALGCGHDNEGLFVGAAGLLGLGRGRLSFPSQTGRRFNHKFSYCLVDRSASSKPSSMV 289

Query: 41  FGSSAVSRKAVFT 3
           FG SAVSR A FT
Sbjct: 290 FGDSAVSRTARFT 302


>ref|XP_002302634.1| predicted protein [Populus trichocarpa] gi|222844360|gb|EEE81907.1|
           predicted protein [Populus trichocarpa]
          Length = 490

 Score =  310 bits (794), Expect = 5e-82
 Identities = 158/253 (62%), Positives = 188/253 (74%), Gaps = 1/253 (0%)
 Frame = -3

Query: 758 TLSLQLHHLDHLNLGDANATGDALFKSRLSRDAARVEALHAISAVKTNSTTTRRRGKXXX 579
           +LS+QLHHLD L+   ++ T   LF SRL+RDA+RV++L +++A   ++  TR RG    
Sbjct: 77  SLSVQLHHLDALS---SDETPQDLFNSRLARDASRVKSLTSLAAAVGSTNRTRARGPGFS 133

Query: 578 XXXXXXXSGLAHGSGEYFTRLGVGTPARYSYMVLDTGSDVVWIQCSPCRNCYTQSDPVFD 399
                   GLA GSGEYFTRLGVGTPARY +MVLDTGSDVVWIQC+PC+ CY+Q+DPVF+
Sbjct: 134 SSVTS---GLAQGSGEYFTRLGVGTPARYVFMVLDTGSDVVWIQCAPCKKCYSQTDPVFN 190

Query: 398 PTKSSTFAGVTCASPLCKRLDSPGCNKGKK-CLYQVSYGDGSFTVGEFSTETMTFRRNRV 222
           PTKS +FA + C SPLC+RLDSPGC+  K  CLYQVSYGDGSFT GEFSTET+TFR  RV
Sbjct: 191 PTKSRSFANIPCGSPLCRRLDSPGCSTKKHICLYQVSYGDGSFTYGEFSTETLTFRGTRV 250

Query: 221 KNIALGCGHDNEGLFVXXXXXXXXXXXXLSFPSQAGPRFGGAFSYCLVDRSASSKPSSLI 42
             +ALGCGHDNEGLF+            LSFPSQ G RF   FSYCLVDRSASSKPS ++
Sbjct: 251 GRVALGCGHDNEGLFIGAAGLLGLGRGRLSFPSQIGRRFSRKFSYCLVDRSASSKPSYMV 310

Query: 41  FGSSAVSRKAVFT 3
           FG SA+SR A FT
Sbjct: 311 FGDSAISRTARFT 323


>ref|XP_002263964.1| PREDICTED: aspartic proteinase nepenthesin-1 [Vitis vinifera]
           gi|147788999|emb|CAN64659.1| hypothetical protein
           VITISV_009613 [Vitis vinifera]
          Length = 489

 Score =  307 bits (787), Expect = 4e-81
 Identities = 158/250 (63%), Positives = 180/250 (72%), Gaps = 5/250 (2%)
 Frame = -3

Query: 737 HLDHLNLGDANATGDALFKSRLSRDAARVEALHAISAVKTNSTTTRRRGKXXXXXXXXXX 558
           HL+H ++   NAT +ALF  RL RDA RVEAL  ++A        RR G+          
Sbjct: 77  HLEHRDVLAFNATPEALFNLRLQRDAFRVEALSKMAAAAGG----RRAGRNGTHAQGGGF 132

Query: 557 S-----GLAHGSGEYFTRLGVGTPARYSYMVLDTGSDVVWIQCSPCRNCYTQSDPVFDPT 393
           S     GLA GSGEYFTRLGVGTP +Y YMVLDTGSDVVWIQC+PCR CY+Q+DPVFDP 
Sbjct: 133 SSSVTSGLAQGSGEYFTRLGVGTPPKYVYMVLDTGSDVVWIQCAPCRKCYSQTDPVFDPK 192

Query: 392 KSSTFAGVTCASPLCKRLDSPGCNKGKKCLYQVSYGDGSFTVGEFSTETMTFRRNRVKNI 213
           KS +F+ ++C SPLC RLDSPGCN  + CLYQV+YGDGSFT GEFSTET+TFR  RV  +
Sbjct: 193 KSGSFSSISCRSPLCLRLDSPGCNSRQSCLYQVAYGDGSFTFGEFSTETLTFRGTRVPKV 252

Query: 212 ALGCGHDNEGLFVXXXXXXXXXXXXLSFPSQAGPRFGGAFSYCLVDRSASSKPSSLIFGS 33
           ALGCGHDNEGLFV            LSFP+Q G RFG  FSYCLVDRSASSKPSS++FG 
Sbjct: 253 ALGCGHDNEGLFVGAAGLLGLGRGRLSFPTQTGLRFGRKFSYCLVDRSASSKPSSVVFGQ 312

Query: 32  SAVSRKAVFT 3
           SAVSR AVFT
Sbjct: 313 SAVSRTAVFT 322