BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00017314
         (1814 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002285061.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   448   e-123
ref|XP_002510140.1| cytochrome P450, putative [Ricinus communis]...   443   e-122
ref|XP_002327185.1| cytochrome P450 [Populus trichocarpa] gi|222...   437   e-120
ref|XP_003538935.1| PREDICTED: cytochrome P450 81D1-like [Glycin...   437   e-120
ref|XP_002283502.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   436   e-119

>ref|XP_002285061.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 508

 Score =  448 bits (1153), Expect = e-123
 Identities = 225/462 (48%), Positives = 306/462 (66%), Gaps = 4/462 (0%)
 Frame = +3

Query: 264  IKEPLHQTLEKLSSKYGNILLLHFGFKKVLVVCSPKAVQECFTTHDTIFANRPVSMATTH 443
            +KEP+H++L+ LS +YG +L L FGF+ VL++ SP AV+ECFT HD +FANRP  +A  H
Sbjct: 50   LKEPVHRSLQHLSDQYGPVLTLQFGFRTVLLLSSPSAVEECFTKHDQVFANRPRLLAGKH 109

Query: 444  LSYDFTTITVAPYGDLWRNLRRLAAVEIFSTNRIDFFTXXXXXXXXXXXXXXXXXXXXXX 623
            L YD+TT+ VAPYG  WRNLRRL  +EIFSTNR++ F                       
Sbjct: 110  LHYDYTTLGVAPYGQHWRNLRRLTTLEIFSTNRLNMFLGIRQDEVKFLLRNLFQRSGQGF 169

Query: 624  XXXXXX--FNELAFNILSMTIGGKRYYGENVEEAEEARKVRFIMREMLDISGNSNVGDFF 797
                     +EL+FNI++  + GKRY+G +VE+ EEA + R I++E+L+ SG SN GDF 
Sbjct: 170  ARVEMKSRLSELSFNIITRIVAGKRYFGTDVEDFEEASRFRDIIKEILETSGASNAGDFL 229

Query: 798  PFLRWFDYGGVEKKMVGFMKSLDLFLQDLIDKRRAIIPMLNDDHEDTANHGKNWKKQTMI 977
            PFL+WFD+ G++K+++   +  D FLQ LID+ R         + + +   +  K +T +
Sbjct: 230  PFLQWFDFQGLKKRVLALQRRTDAFLQGLIDEGR---------NHNNSYRQERGKTKTFV 280

Query: 978  DHLLLMQDSEPENYSDKIIKGIIMVLLIAGTDTLSLTLEWAMALLLNHPESMEKVRAEIE 1157
            D +L +Q+SEPE Y+D IIKG+I+ LL AGTDT ++T+EWAM+LLLNHP  ++KV+ E++
Sbjct: 281  DSMLALQNSEPEYYADHIIKGMILTLLTAGTDTSAVTMEWAMSLLLNHPTVLDKVKTELD 340

Query: 1158 THVPKDRLLVEQDLSKLNYLQNVITETLRLHPPLPFLIPHECSADCTVGGYNVSRGTMLL 1337
              +   RL+ E DLS L YL+ ++ ETLRL P  P L+ HE S DC++GGY+V  GTMLL
Sbjct: 341  CKIGHQRLVEEPDLSDLPYLRAIVNETLRLFPAAPLLVAHESSDDCSIGGYDVRGGTMLL 400

Query: 1338 VNVRAIHRDPTVWSKPNEFIPERHE-CQGEE-MMIPFGSGRRGCPGAGLGKRVLGLALGR 1511
            VN  AIHRD  VW  P  F PER E  +GE    IPFG GRRGCPGAGL  RV+GLAL  
Sbjct: 401  VNAWAIHRDAKVWEDPTSFRPERFEGGEGEACRFIPFGLGRRGCPGAGLANRVMGLALAA 460

Query: 1512 LIQVFEWERIGVELEDMTEGPGFSTPKIKPLEAFCKPRAATV 1637
            L+Q FEW+R+G    DM+EG G + PK +PLEA C+ R + +
Sbjct: 461  LVQCFEWQRVGEVEVDMSEGKGLTMPKAQPLEAMCRARNSMI 502


>ref|XP_002510140.1| cytochrome P450, putative [Ricinus communis]
            gi|223550841|gb|EEF52327.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 504

 Score =  443 bits (1140), Expect = e-122
 Identities = 233/512 (45%), Positives = 319/512 (62%), Gaps = 13/512 (2%)
 Frame = +3

Query: 141  MEETYWYLVCIIFLMYFLLMKKQ---------KNNXXXXXXXXXXXXXXXIKEPLHQTLE 293
            MEET+   VCI+F  + +L+  +         +N                +KEP+H++L+
Sbjct: 1    MEETW---VCILFTTFVILLLSRHFWSKRTTYRNLPPSPPKLPIIGHLHLMKEPVHRSLQ 57

Query: 294  KLSSKYGNILLLHFGFKKVLVVCSPKAVQECFTTHDTIFANRPVSMATTHLSYDFTTITV 473
             LS+KYG I+ L FG + V+++ SP  V+ECFT +D + A+RP   A  +L YD+TTI  
Sbjct: 58   DLSNKYGPIIFLSFGSQPVIIISSPSLVEECFTKNDIVLADRPRRQAGKYLHYDYTTIGA 117

Query: 474  APYGDLWRNLRRLAAVEIFSTNRIDFFTXXXXXXXXXXXXXXXXXXXXXXXXXXXXFN-- 647
            A YGDLWRNLRRLA VEI STNR++ F                                 
Sbjct: 118  ANYGDLWRNLRRLATVEILSTNRLNMFHGIRQEEVRMLVKNLFQSAGQVSAKVEMKSRLV 177

Query: 648  ELAFNILSMTIGGKRYYGENVEEAEEARKVRFIMREMLDISGNSNVGDFFPFLRWFDYGG 827
             L+FNI+   + GKRY+G  V++ EEA +   ++RE   +SG +N+GDFFP +RW DY G
Sbjct: 178  GLSFNIIMRMVAGKRYFGSEVKDVEEATQFHDVIRETFVLSGAANLGDFFPLIRWLDYRG 237

Query: 828  VEKKMVGFMKSLDLFLQDLIDKRRAIIPMLNDDHEDTANHGKNWKKQTMIDHLLLMQDSE 1007
            +EK++V   K++DL  Q LID+ R            +    K+ K  TMID +L +Q+ +
Sbjct: 238  IEKRLVSARKNMDLLFQRLIDEHR--------HKRGSCLEDKSCK--TMIDVVLSLQEFQ 287

Query: 1008 PENYSDKIIKGIIMVLLIAGTDTLSLTLEWAMALLLNHPESMEKVRAEIETHVPKDRLLV 1187
            PE YSD+IIKG+IM +L AGTDT ++T+EWAM+LLLNHP+++ K RAE++ HV +DRL+ 
Sbjct: 288  PEYYSDEIIKGLIMAMLTAGTDTSAVTIEWAMSLLLNHPKALTKARAELDIHVGQDRLVD 347

Query: 1188 EQDLSKLNYLQNVITETLRLHPPLPFLIPHECSADCTVGGYNVSRGTMLLVNVRAIHRDP 1367
            EQDL KL YL  +I ETLRL P  P L+PH+ S DC +GG+++ +GT+L VN  A+HRDP
Sbjct: 348  EQDLPKLQYLHCIINETLRLFPAAPLLVPHKSSDDCKIGGFDIPQGTVLSVNAWALHRDP 407

Query: 1368 TVWSKPNEFIPERHECQGEE--MMIPFGSGRRGCPGAGLGKRVLGLALGRLIQVFEWERI 1541
             +W  PN F PER E    E  +++PFG GRR CPGAGL  RV+GLAL  LIQ F+WERI
Sbjct: 408  KIWEDPNSFRPERFEGIKYETCLLVPFGLGRRSCPGAGLANRVVGLALAALIQCFDWERI 467

Query: 1542 GVELEDMTEGPGFSTPKIKPLEAFCKPRAATV 1637
              E  DM EGPG + PK++PLEA CK R + +
Sbjct: 468  TEEEIDMLEGPGLTMPKVQPLEAMCKIRESMI 499


>ref|XP_002327185.1| cytochrome P450 [Populus trichocarpa] gi|222835500|gb|EEE73935.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 601

 Score =  437 bits (1125), Expect = e-120
 Identities = 227/506 (44%), Positives = 321/506 (63%), Gaps = 11/506 (2%)
 Frame = +3

Query: 141  MEETYWYLVCIIFLMYFLLMK-------KQKNNXXXXXXXXXXXXXXXIKEPLHQTLEKL 299
            ME ++W+    +F  + L  K         KN                +K+P+H+TL +L
Sbjct: 1    MEVSHWFNFAALFFFFVLASKLVIYKLGNPKNLPPSPPSRPIIGHLHLLKQPIHRTLCEL 60

Query: 300  SSKYGNILLLHFGFKKVLVVCSPKAVQECFTTHDTIFANRPVSMATTHLSYDFTTITVAP 479
            S KYG+IL L FG +KVLV+ SP AV+ECFT  D IFANRP ++A  HL+Y+ TT+  + 
Sbjct: 61   SKKYGDILFLRFGARKVLVISSPSAVEECFTRKDVIFANRPRTLAGKHLNYNSTTMGFSS 120

Query: 480  YGDLWRNLRRLAAVEIFSTNRIDFFTXXXXXXXXXXXXXXXXXXXXXXXXXXXX--FNEL 653
            YG+ WRNLRRL  +E+FS +R+  F+                              F EL
Sbjct: 121  YGEHWRNLRRLTTIELFSASRVASFSDIRKEEVQLLLNQLFRDSSKQQAKVGLTASFMEL 180

Query: 654  AFNILSMTIGGKRYYGENVEEAEEARKVRFIMREMLDISGNSNVGDFFPFLRWFDYGGVE 833
             FN++   I GKRYYG+ V + EEA + + I++EM  + G+SN+ DFFP L+W D+ G+E
Sbjct: 181  TFNVMMRMIAGKRYYGKEVVD-EEAGQFQNIIKEMEALRGSSNMNDFFPVLQWIDFQGLE 239

Query: 834  KKMVGFMKSLDLFLQDLIDKRRAIIPMLNDDHEDTANHGKNWKKQTMIDHLLLMQDSEPE 1013
            K+M+G  K +D FLQDLI++ + +    +   + T   G   +  T+ID +L ++++EPE
Sbjct: 240  KRMMGLKKKMDKFLQDLIEEHQKVRSQSSQSTKITGL-GNQKRNMTLIDVMLSLKETEPE 298

Query: 1014 NYSDKIIKGIIMVLLIAGTDTLSLTLEWAMALLLNHPESMEKVRAEIETHVPKDRLLVEQ 1193
             Y+D+ IKG+IM  L AG+ T + TLEWAM+LLLN+PE+M K   EI+  V  + +L E 
Sbjct: 299  FYTDQTIKGVIMSTLTAGSQTSAATLEWAMSLLLNNPETMRKASEEIDAIVGTEHILDEV 358

Query: 1194 DLSKLNYLQNVITETLRLHPPLPFLIPHECSADCTVGGYNVSRGTMLLVNVRAIHRDPTV 1373
            D++KL+YLQN++ ET RL PP P L+PHE S DCT+ G++V RGTMLLVN  +IHRD  +
Sbjct: 359  DVTKLSYLQNILNETFRLFPPAPLLLPHESSEDCTISGFHVPRGTMLLVNTWSIHRDTKL 418

Query: 1374 WSKPNEFIPERHE-CQGE-EMMIPFGSGRRGCPGAGLGKRVLGLALGRLIQVFEWERIGV 1547
            W +P +F+PER E  +GE   ++PFG+GRR CPGAGL KR++GL LG LIQ FEW+R+  
Sbjct: 419  WVEPTKFMPERFEGGEGEGYKLLPFGAGRRACPGAGLAKRIIGLTLGVLIQCFEWDRVSK 478

Query: 1548 ELEDMTEGPGFSTPKIKPLEAFCKPR 1625
            E  ++TEG G + PK +PLEA C+PR
Sbjct: 479  EEINLTEGTGLTIPKAEPLEALCRPR 504



 Score = 75.9 bits (185), Expect = 3e-11
 Identities = 38/75 (50%), Positives = 47/75 (62%)
 Frame = +3

Query: 1413 CQGEEMMIPFGSGRRGCPGAGLGKRVLGLALGRLIQVFEWERIGVELEDMTEGPGFSTPK 1592
            C+  + M+   S  R CPGAGL  RV+GLAL  LIQ FEWERI  E  DM+EG G +  K
Sbjct: 523  CRPRQSMVNLLSSMRSCPGAGLANRVVGLALASLIQCFEWERIREEEVDMSEGSGITMLK 582

Query: 1593 IKPLEAFCKPRAATV 1637
             KPL+A  K R + +
Sbjct: 583  AKPLKAMFKARMSMI 597


>ref|XP_003538935.1| PREDICTED: cytochrome P450 81D1-like [Glycine max]
          Length = 580

 Score =  437 bits (1123), Expect = e-120
 Identities = 229/501 (45%), Positives = 319/501 (63%), Gaps = 8/501 (1%)
 Frame = +3

Query: 159  YLVCIIFLMYFLLMKKQKNNXXXXXXXXXXXXXXXIKEPLHQTLEKLSSKYGNILLLHFG 338
            +L+  ++++  +L+K +                  IKEPLH +L KL+ KYG I+ L  G
Sbjct: 83   FLLLFLYVLKSILLKSKNLPPSPPYALPLIGHLHLIKEPLHLSLHKLTDKYGPIIFLCLG 142

Query: 339  FKKVLVVCSPKAVQECFTTHDTIFANRPVSMATTHLSYDFTTITVAPYGDLWRNLRRLAA 518
             +KVLVV SP AV+ECFT +D  FANRP ++A  HL+Y+ TTI VA YG  WRNLRRL  
Sbjct: 143  TRKVLVVSSPSAVEECFTKNDITFANRPQTLAAKHLNYNKTTIGVASYGHYWRNLRRLTT 202

Query: 519  VEIFSTNRIDFFTXXXXXXXXXXXXXXXXXXXXXXXXXXXX---FNELAFNILSMTIGGK 689
            VE+FST R+   T                                 E++FNI+   I GK
Sbjct: 203  VELFSTTRLAMLTSVRVEEVQLMVKQLFEECKGRQQIMIDLRARLLEVSFNIMLRMISGK 262

Query: 690  RYYGENVEEAEEARKVRFIMREMLDISGNSNVGDFFPFLRWFDYGGVEKKMVGFMKSLDL 869
            RYYG++   A+E ++ + +M+E +++ G+ N+ DFFP L+W D+GGVEKKMV  MK +D 
Sbjct: 263  RYYGKHAI-AQEGKEFQILMKEFVELLGSGNLNDFFPLLQWVDFGGVEKKMVKLMKKMDS 321

Query: 870  FLQDLIDKRRAIIPMLNDDHEDTANHGKNWKKQTMIDHLLLMQDSEPENYSDKIIKGIIM 1049
            FLQ L+D+      +++++ ++        K  T+ID +L +Q +EPE Y+ + +KG+I+
Sbjct: 322  FLQKLLDEHCTRRNVMSEEEKERR------KSMTLIDVMLDLQQTEPEFYTHETVKGVIL 375

Query: 1050 VLLIAGTDTLSLTLEWAMALLLNHPESMEKVRAEIETHVPKDRLLVEQDLSKLNYLQNVI 1229
             +L+AG++T + T+EWA +LLLNHP+ M KV+ EI+T+V +D++L   D +KL YLQNVI
Sbjct: 376  AMLVAGSETSATTMEWAFSLLLNHPKKMNKVKEEIDTYVGQDQMLNGLDTTKLKYLQNVI 435

Query: 1230 TETLRLHPPLPFLIPHECSADCTVGGYNVSRGTMLLVNVRAIHRDPTVWSKPNEFIPERH 1409
            TETLRL+P  P L+PHE S DC V G+++ RGTMLLVN+  +HRD  +W  P  F+PER 
Sbjct: 436  TETLRLYPVAPLLLPHESSNDCKVCGFDIPRGTMLLVNLWTLHRDANLWVDPAMFVPERF 495

Query: 1410 ECQGEEM-----MIPFGSGRRGCPGAGLGKRVLGLALGRLIQVFEWERIGVELEDMTEGP 1574
            E  GEE      MIPFG GRR CPGA L KRV+G ALG LIQ FEWERIG +  DMTEG 
Sbjct: 496  E--GEEADEVYNMIPFGIGRRACPGAVLAKRVMGHALGTLIQCFEWERIGHQEIDMTEGI 553

Query: 1575 GFSTPKIKPLEAFCKPRAATV 1637
            G + PK++PL A C+PR + +
Sbjct: 554  GLTMPKLEPLVALCRPRQSMI 574


>ref|XP_002283502.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 508

 Score =  436 bits (1121), Expect = e-119
 Identities = 225/505 (44%), Positives = 312/505 (61%), Gaps = 12/505 (2%)
 Frame = +3

Query: 147  ETYWYLVCIIFLMYFLLMK--------KQKNNXXXXXXXXXXXXXXXIKEPLHQTLEKLS 302
            E  W    + FL + L +K        K+ N                +K PLH+TL +LS
Sbjct: 2    EARWLYSSLSFLFFALAVKFLLQRNKGKRLNLPPSPPGFPIFGHLHLLKGPLHRTLHRLS 61

Query: 303  SKYGNILLLHFGFKKVLVVCSPKAVQECFTTHDTIFANRPVSMATTHLSYDFTTITVAPY 482
             ++G I+ L FG + V+VV SP AV+ECFT +D IFANRP  +   ++ YD+T +++APY
Sbjct: 62   ERHGPIVSLRFGSRPVIVVSSPSAVEECFTKNDVIFANRPKFVMGKYIGYDYTVVSLAPY 121

Query: 483  GDLWRNLRRLAAVEIFSTNRIDFFTXXXXXXXXXXXXXXXXXXXXXXXXXXXX--FNELA 656
            GD WRNLRRL+AVEIF++NR++ F                               F+EL 
Sbjct: 122  GDHWRNLRRLSAVEIFASNRLNLFLGIRRDEIKQLLLRLSRNSVENFAKVELKSMFSELL 181

Query: 657  FNILSMTIGGKRYYGENVEEAEEARKVRFIMREMLDISGNSNVGDFFPFLRWFDYGGVEK 836
             NI    + GKR+YG+N+++ EEAR+ R I +E+L+ SG SN GDF P L+W DY G  K
Sbjct: 182  LNITMRMVAGKRFYGDNMKDVEEAREFREISKEILEFSGTSNPGDFLPILQWIDYQGYNK 241

Query: 837  KMVGFMKSLDLFLQDLIDKRRAIIPMLNDDHEDTANHGKNWKKQTMIDHLLLMQDSEPEN 1016
            + +   K +D+FLQ L+D+ R+      +   D  N      + TMIDHLL +Q+SEPE 
Sbjct: 242  RALRLGKKMDVFLQGLLDECRS------NKRSDLEN------RNTMIDHLLSLQESEPEY 289

Query: 1017 YSDKIIKGIIMVLLIAGTDTLSLTLEWAMALLLNHPESMEKVRAEIETHVPKDRLLVEQD 1196
            Y+D+IIKG+I+ + + G DT ++T+EWAM+LLLNHPE ++K R E++TH+  D L+ E D
Sbjct: 290  YTDEIIKGLIVAMQVGGADTTAVTIEWAMSLLLNHPEVLKKARDELDTHIGHDCLIDETD 349

Query: 1197 LSKLNYLQNVITETLRLHPPLPFLIPHECSADCTVGGYNVSRGTMLLVNVRAIHRDPTVW 1376
            L KL YLQ++I+E+LRL P  P L+PH  + DC + G++V  GTMLLVN  A+HRDP +W
Sbjct: 350  LPKLQYLQSIISESLRLFPSTPLLVPHFSTEDCKLRGFDVPGGTMLLVNAWALHRDPKLW 409

Query: 1377 SKPNEFIPERHECQGEE--MMIPFGSGRRGCPGAGLGKRVLGLALGRLIQVFEWERIGVE 1550
            + P  F PER E    E   ++PFG GRR CPG GL  RV+GL LG LIQ F+W+R+  +
Sbjct: 410  NDPTSFKPERFETGESETYKLLPFGVGRRACPGIGLANRVMGLTLGSLIQCFDWKRVDEK 469

Query: 1551 LEDMTEGPGFSTPKIKPLEAFCKPR 1625
              DM EG G + PK++PLEA CK R
Sbjct: 470  EIDMAEGQGLTMPKVEPLEAMCKTR 494