BLASTX nr result
ID: Bupleurum21_contig00017109
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00017109
(1287 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAB91554.1| beta 1-3 glucanase [Vitis vinifera] 312 1e-82
ref|XP_002277511.1| PREDICTED: glucan endo-1,3-beta-glucosidase,... 312 1e-82
gb|ACD45060.1| beta-1,3-glucanase [Vitis riparia] 310 7e-82
gb|AAF33405.1|AF230109_1 beta-1,3 glucanase [Populus tremula x P... 309 1e-81
gb|ADW08746.1| 1,3-beta-D-glucanase GH17_101 [Populus tremula x ... 309 1e-81
>emb|CAB91554.1| beta 1-3 glucanase [Vitis vinifera]
Length = 345
Score = 312 bits (800), Expect = 1e-82
Identities = 172/363 (47%), Positives = 231/363 (63%), Gaps = 2/363 (0%)
Frame = +2
Query: 44 SIMMLILFSIVAFLHATSGATVRHGPEPGPQPVGVCHGTFATNQPSAQQAVSLLQSAGIR 223
+I++L+ ++A LH T T GVC+G N P Q V L IR
Sbjct: 15 AILLLVFGLLMASLHLTVAQT------------GVCYGMEGDNLPPPGQVVGLYNQYSIR 62
Query: 224 RVRFYGPDYDAFEALRNTGIEVVLGVPNNDLQFIATSQANANDWIEQNIESYEDIKFRYI 403
R+R Y P+ A +ALR + IE+++GVPN+ LQ IA+ Q NAN W++ NI++Y +++FRY+
Sbjct: 63 RMRLYDPNQAALQALRGSNIELMIGVPNDALQNIASIQGNANSWVQNNIKNYGNVRFRYV 122
Query: 404 VVGNGISPYQFGGTPQHTPFLLDAMQNIQNGLSSSAKFNHIPVTTAIDQSEALDESFPPS 583
VGN +SP G T Q F+L AM+NI N +S++ N I V+TAID + L S+PPS
Sbjct: 123 AVGNEVSP--TGPTAQ---FVLPAMRNIFNAISAAGLGNQIKVSTAID-TGVLGTSYPPS 176
Query: 584 KGHFNREYLQFFNPVISFLVKYRTPLLVNLHPYYSYLHNKGDIPLDLKKGISISQDPRLQ 763
KG F E F NP+ISFLVK R PLLVNL+PY+SY+ N DI RL
Sbjct: 177 KGAFKPEVTSFLNPIISFLVKNRAPLLVNLYPYFSYIGNTRDI--------------RLD 222
Query: 764 YVIFQRINPIVQDGPFAYTNIFDAMVDSVYSALEKAGGSSLDVVVSEIGWPTADGEGASN 943
Y +F+ +VQDG Y N+FDA++D+VYSALE+ GG SL VV+SE GWP+A G +
Sbjct: 223 YALFKAPGVVVQDGQLGYKNLFDAILDAVYSALERVGGGSLQVVISESGWPSAGGTATTV 282
Query: 944 YIAATHNNNLINHVHKSGTPRRPQKFIETYIFSLFDENKR--EDNKHWGVFLNNKQAKYR 1117
A T+N+NLI HV K GTP++P IETY+F++F+EN++ E KHWG+FL NKQAKY
Sbjct: 283 GNAKTYNSNLIQHV-KGGTPKKPGGPIETYVFAMFNENRKSPEYEKHWGLFLPNKQAKYP 341
Query: 1118 INF 1126
INF
Sbjct: 342 INF 344
>ref|XP_002277511.1| PREDICTED: glucan endo-1,3-beta-glucosidase, basic isoform [Vitis
vinifera]
Length = 345
Score = 312 bits (799), Expect = 1e-82
Identities = 172/361 (47%), Positives = 230/361 (63%), Gaps = 2/361 (0%)
Frame = +2
Query: 50 MMLILFSIVAFLHATSGATVRHGPEPGPQPVGVCHGTFATNQPSAQQAVSLLQSAGIRRV 229
++L+ ++A LH T T GVC+G N P Q V L IRR+
Sbjct: 17 LLLVFGLLMASLHLTVAQT------------GVCYGMEGDNLPPPGQVVGLYNQYSIRRM 64
Query: 230 RFYGPDYDAFEALRNTGIEVVLGVPNNDLQFIATSQANANDWIEQNIESYEDIKFRYIVV 409
R Y P+ A +ALR + IE+++GVPN+ LQ IA+ Q NAN W++ NI++Y +++FRY+ V
Sbjct: 65 RLYDPNQAALQALRGSNIELMIGVPNDALQNIASIQGNANSWVQNNIKNYGNVRFRYVAV 124
Query: 410 GNGISPYQFGGTPQHTPFLLDAMQNIQNGLSSSAKFNHIPVTTAIDQSEALDESFPPSKG 589
GN +SP G T Q F+L AM+NI N +S++ N I V+TAID + L S+PPSKG
Sbjct: 125 GNEVSP--TGPTAQ---FVLPAMRNIFNAISAAGLGNQIKVSTAID-TGVLGTSYPPSKG 178
Query: 590 HFNREYLQFFNPVISFLVKYRTPLLVNLHPYYSYLHNKGDIPLDLKKGISISQDPRLQYV 769
F E F NP+ISFLVK R PLLVNL+PY+SY+ N DI RL Y
Sbjct: 179 AFKPEVTSFLNPIISFLVKNRAPLLVNLYPYFSYIGNTRDI--------------RLDYA 224
Query: 770 IFQRINPIVQDGPFAYTNIFDAMVDSVYSALEKAGGSSLDVVVSEIGWPTADGEGASNYI 949
+F+ +VQDG Y N+FDA++D+VYSALE+AGG SL VV+SE GWP+A G +
Sbjct: 225 LFKAPGVVVQDGQLGYRNLFDAILDAVYSALERAGGGSLQVVISESGWPSAGGTATTVDN 284
Query: 950 AATHNNNLINHVHKSGTPRRPQKFIETYIFSLFDENKR--EDNKHWGVFLNNKQAKYRIN 1123
A T+N+NLI HV K GTP++P IETY+F++F+EN++ E KHWG+FL NKQAKY IN
Sbjct: 285 AKTYNSNLIQHV-KGGTPKKPGGPIETYVFAMFNENRKSPEYEKHWGLFLPNKQAKYPIN 343
Query: 1124 F 1126
F
Sbjct: 344 F 344
>gb|ACD45060.1| beta-1,3-glucanase [Vitis riparia]
Length = 345
Score = 310 bits (793), Expect = 7e-82
Identities = 171/361 (47%), Positives = 227/361 (62%), Gaps = 2/361 (0%)
Frame = +2
Query: 50 MMLILFSIVAFLHATSGATVRHGPEPGPQPVGVCHGTFATNQPSAQQAVSLLQSAGIRRV 229
++L+ ++A LH T T GVC+G N P Q V L IRR+
Sbjct: 17 LLLVFGLLMASLHLTVAQT------------GVCYGMQGDNLPPPGQVVGLYNQYSIRRM 64
Query: 230 RFYGPDYDAFEALRNTGIEVVLGVPNNDLQFIATSQANANDWIEQNIESYEDIKFRYIVV 409
R Y P+ A +ALR + IE+++GVPN+ LQ IA+SQ NAN W++ NI++Y +++FRY+ V
Sbjct: 65 RLYDPNQAALQALRGSNIELMIGVPNDALQNIASSQGNANSWVQNNIKNYGNVRFRYVAV 124
Query: 410 GNGISPYQFGGTPQHTPFLLDAMQNIQNGLSSSAKFNHIPVTTAIDQSEALDESFPPSKG 589
GN +SP G T Q F+L AM+NI N +S++ N I V+TAID + L S+PPS+G
Sbjct: 125 GNEVSP--TGSTAQ---FVLPAMRNIFNAISAAGLGNQIKVSTAID-TGVLGTSYPPSQG 178
Query: 590 HFNREYLQFFNPVISFLVKYRTPLLVNLHPYYSYLHNKGDIPLDLKKGISISQDPRLQYV 769
F E F NP+ISFLV R PLLVNL+PY+SY+ N DI RL Y
Sbjct: 179 AFKPEVTSFLNPIISFLVNNRAPLLVNLYPYFSYIGNTRDI--------------RLDYA 224
Query: 770 IFQRINPIVQDGPFAYTNIFDAMVDSVYSALEKAGGSSLDVVVSEIGWPTADGEGASNYI 949
+F +VQDG Y N+FDA++D+VYSALEKAGG SL VV+SE GWP+A G +
Sbjct: 225 LFTAPGVVVQDGQLGYRNLFDAILDAVYSALEKAGGGSLQVVISESGWPSAGGTATTVDN 284
Query: 950 AATHNNNLINHVHKSGTPRRPQKFIETYIFSLFDENKR--EDNKHWGVFLNNKQAKYRIN 1123
A T+N+NLI HV K GTP++P IETY+F++FDEN++ E KHWG+F NKQ KY IN
Sbjct: 285 AKTYNSNLIQHV-KGGTPKKPGGPIETYVFAMFDENRKSPEYEKHWGLFSPNKQPKYPIN 343
Query: 1124 F 1126
F
Sbjct: 344 F 344
>gb|AAF33405.1|AF230109_1 beta-1,3 glucanase [Populus tremula x Populus alba]
Length = 343
Score = 309 bits (791), Expect = 1e-81
Identities = 164/332 (49%), Positives = 217/332 (65%), Gaps = 2/332 (0%)
Frame = +2
Query: 140 VGVCHGTFATNQPSAQQAVSLLQSAGIRRVRFYGPDYDAFEALRNTGIEVVLGVPNNDLQ 319
+GVC+G N P AQ+ + L GIRR+R Y P+ DA AL+ T IE++LGVPN DLQ
Sbjct: 34 IGVCYGMNG-NLPPAQEVIELYSQRGIRRMRLYDPNQDALRALQGTNIELMLGVPNADLQ 92
Query: 320 FIATSQANANDWIEQNIESYEDIKFRYIVVGNGISPYQFGGTPQHTPFLLDAMQNIQNGL 499
IA+SQ NAN W+++N+ S+ +++FRYI VGN + P+ + FL+ AM+NI+N L
Sbjct: 93 RIASSQTNANAWVQRNVRSFGNVRFRYIAVGNEVRPFD-----SYAQFLVPAMKNIRNAL 147
Query: 500 SSSAKFNHIPVTTAIDQSEALDESFPPSKGHFNREYLQFFNPVISFLVKYRTPLLVNLHP 679
SA +I V+TAID D+S PPSKG F ++ F +P+I FL+ + PLLVNL+P
Sbjct: 148 -YSAGLGNIKVSTAIDNGVIEDDSSPPSKGSFRGDHRPFLDPIIRFLLNNQAPLLVNLYP 206
Query: 680 YYSYLHNKGDIPLDLKKGISISQDPRLQYVIFQRINPIVQDGPFAYTNIFDAMVDSVYSA 859
Y SY N S+D RL Y +F + +V D P Y N+FDA++D+VY+A
Sbjct: 207 YLSYTGN--------------SEDIRLDYALFTAPSSLVSDPPLNYQNLFDAILDTVYAA 252
Query: 860 LEKAGGSSLDVVVSEIGWPTADGEGASNYIAATHNNNLINHVHKSGTPRRPQKFIETYIF 1039
LEK+GG SLD+VVSE GWPTA G G S A +NNNL+ HV K GTP++P K IETYIF
Sbjct: 253 LEKSGGGSLDIVVSESGWPTAGGTGTSVDNARIYNNNLVQHV-KRGTPKKPGKPIETYIF 311
Query: 1040 SLFDENKR--EDNKHWGVFLNNKQAKYRINFQ 1129
S+FDE + E KHWG+FL NKQ KY IN +
Sbjct: 312 SMFDETYKNPELEKHWGIFLPNKQPKYNINLR 343
>gb|ADW08746.1| 1,3-beta-D-glucanase GH17_101 [Populus tremula x Populus tremuloides]
Length = 343
Score = 309 bits (791), Expect = 1e-81
Identities = 164/332 (49%), Positives = 217/332 (65%), Gaps = 2/332 (0%)
Frame = +2
Query: 140 VGVCHGTFATNQPSAQQAVSLLQSAGIRRVRFYGPDYDAFEALRNTGIEVVLGVPNNDLQ 319
+GVC+G N P AQ+ + L GIRR+R Y P+ DA AL+ T IE++LGVPN DLQ
Sbjct: 34 IGVCYGMNG-NLPPAQEVIELYSQRGIRRMRLYDPNQDALRALQGTNIELMLGVPNADLQ 92
Query: 320 FIATSQANANDWIEQNIESYEDIKFRYIVVGNGISPYQFGGTPQHTPFLLDAMQNIQNGL 499
IA+SQ NAN W+++N+ S+ +++FRYI VGN + P+ + FL+ AM+NI+N L
Sbjct: 93 RIASSQTNANAWVQRNVRSFGNVRFRYIAVGNEVRPFD-----SYAQFLVPAMKNIRNAL 147
Query: 500 SSSAKFNHIPVTTAIDQSEALDESFPPSKGHFNREYLQFFNPVISFLVKYRTPLLVNLHP 679
SA +I V+TAID D+S PPSKG F ++ F +P+I FL+ + PLLVNL+P
Sbjct: 148 -YSAGLGNIKVSTAIDNGVIEDDSSPPSKGSFRGDHRPFLDPIIRFLLNNQAPLLVNLYP 206
Query: 680 YYSYLHNKGDIPLDLKKGISISQDPRLQYVIFQRINPIVQDGPFAYTNIFDAMVDSVYSA 859
Y SY N S+D RL Y +F + +V D P Y N+FDA++D+VY+A
Sbjct: 207 YLSYTGN--------------SEDIRLDYALFTAPSSLVSDPPLNYQNLFDAILDTVYAA 252
Query: 860 LEKAGGSSLDVVVSEIGWPTADGEGASNYIAATHNNNLINHVHKSGTPRRPQKFIETYIF 1039
LEK+GG SLD+VVSE GWPTA G G S A +NNNL+ HV K GTP++P K IETYIF
Sbjct: 253 LEKSGGGSLDIVVSESGWPTAGGTGTSVDNARIYNNNLVQHV-KRGTPKKPGKPIETYIF 311
Query: 1040 SLFDENKR--EDNKHWGVFLNNKQAKYRINFQ 1129
S+FDE + E KHWG+FL NKQ KY IN +
Sbjct: 312 SMFDETYKNPELEKHWGIFLPNKQPKYNINLR 343