BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00016296
         (2443 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002264607.1| PREDICTED: DNA topoisomerase 3-alpha [Vitis ...   959   0.0  
ref|XP_002307047.1| predicted protein [Populus trichocarpa] gi|2...   900   0.0  
ref|XP_004136599.1| PREDICTED: DNA topoisomerase 3-alpha-like [C...   882   0.0  
ref|XP_002527391.1| prokaryotic DNA topoisomerase, putative [Ric...   905   0.0  
ref|NP_201197.1| DNA topoisomerase III [Arabidopsis thaliana] gi...   867   0.0  

>ref|XP_002264607.1| PREDICTED: DNA topoisomerase 3-alpha [Vitis vinifera]
          Length = 914

 Score =  959 bits (2480), Expect(2) = 0.0
 Identities = 451/554 (81%), Positives = 502/554 (90%)
 Frame = -1

Query: 2443 QWLILWLDCDREGENIAFEVVQVCTEANRQLNVWRARFSSIIEREIHQSVQRLGRPNQLF 2264
            QWL+LWLDCDREGENIAFEV++VC + NR ++VWRARFS++I+REIH+SVQ L RPNQLF
Sbjct: 109  QWLVLWLDCDREGENIAFEVLEVCAQVNRHIDVWRARFSALIDREIHESVQNLVRPNQLF 168

Query: 2263 SDAVDVRQEIDLRIGASFTRFQTMLLRDAFVLDFAADGRNVVLSYGPCQFPTLGFVVERY 2084
            +DAVDVRQEIDLRIGASFTRFQTMLLRDAFVLDFAAD RN+VLSYGPCQFPTLGFVVERY
Sbjct: 169  ADAVDVRQEIDLRIGASFTRFQTMLLRDAFVLDFAADDRNLVLSYGPCQFPTLGFVVERY 228

Query: 2083 WEIQSHEPEEFWAINCTHNSDNCTASLNWMRGHLFDYTCAVIIYEMCIEEPTATVTNVRQ 1904
            WEIQSHEPEEFW INC+H SD  T++ NWMR HLFDYTCAVIIYEMCI+EPTATVT VRQ
Sbjct: 229  WEIQSHEPEEFWTINCSHKSDEGTSTFNWMRAHLFDYTCAVIIYEMCIQEPTATVTKVRQ 288

Query: 1903 RETLKRPPHPLSTIELEKRASRYFRMGAKQTMKIAEELYLSGFISYPRTETDSFSSQADL 1724
            +E LK PPHPL+TIELEKRASRYF M ++ TMK+AE+LY +GFISYPRTETD FS + DL
Sbjct: 289  QEKLKYPPHPLNTIELEKRASRYFHMSSEHTMKVAEDLYQTGFISYPRTETDYFSERTDL 348

Query: 1723 HTVVRDQQQHPVWGSYAQRLLDPEAGLWKNPSNGGHDDKAHPPIHPTKFSSGESNWTPDH 1544
            H +V++Q  HP+WGSYAQRLLDP AGLW+NPSNGGHDDKAHPPIHPTKFS+GES W+ DH
Sbjct: 349  HGIVQEQLGHPIWGSYAQRLLDPGAGLWRNPSNGGHDDKAHPPIHPTKFSAGESGWSQDH 408

Query: 1543 HKLYELVVRHFLACVSQPAVGAETTVDIDIAGELFCTTGRVILAKNYLEVYRFESWGGSI 1364
             +LYELVVRHFLACVSQPAVGAET V+IDIAGE F ++GRVI+AKNYL+VYRFESWGGS+
Sbjct: 409  QRLYELVVRHFLACVSQPAVGAETIVEIDIAGESFSSSGRVIIAKNYLDVYRFESWGGSM 468

Query: 1363 IPTYTLGQQFIPTTLTLDSGVTRPPPLLSEADLMECMDKAGIGTDATMHDHIQKLLDRYY 1184
            IPTY  GQQFIPTTLTLDSGVTRPPPLLSEADL+ CMDKAGIGTDATMHDHI+KLLDR+Y
Sbjct: 469  IPTYVFGQQFIPTTLTLDSGVTRPPPLLSEADLLNCMDKAGIGTDATMHDHIKKLLDRFY 528

Query: 1183 AIKDLNTTRFTPTNLGEALVMGYDDMGYELWKPYLRAMMESDMKAVSVGTKNKAEVLATC 1004
            A KD N TRF+PTNLGEALVMGYDDMGYELWKPYLR+MME DMKAVS+GTK + EVL TC
Sbjct: 529  ATKDSN-TRFSPTNLGEALVMGYDDMGYELWKPYLRSMMECDMKAVSIGTKRRNEVLTTC 587

Query: 1003 LEQMKACFVDARLKKVKLFEAMDVFFERSNRPVTNEQHTFGDVVRRCDRCRESDMVLKRK 824
            LEQMKACF+DARL KVKL EAM +FFERSNR   +EQH  GDVVR+C  C+ESDMVL+RK
Sbjct: 588  LEQMKACFLDARLNKVKLLEAMALFFERSNRSSGDEQHEVGDVVRQCTLCQESDMVLRRK 647

Query: 823  PDGNFMVGCLGYPQ 782
            PDGNFMVGCLGYPQ
Sbjct: 648  PDGNFMVGCLGYPQ 661



 Score =  226 bits (575), Expect(2) = 0.0
 Identities = 120/256 (46%), Positives = 145/256 (56%), Gaps = 3/256 (1%)
 Frame = -2

Query: 783  KNAVWLPGPISEAXXXXXXXXXXTPGPVLKIQFKFRRMEIPMHFDVEHLGCIGGCDDILK 604
            +N VWLPG ISEA          TPGPV KIQFKFRR+EIP +++++HLGC+ GCD+IL+
Sbjct: 663  RNVVWLPGSISEAAITTISCNHCTPGPVYKIQFKFRRLEIPPNYNIDHLGCVCGCDEILR 722

Query: 603  QLIETCGTGSRDILGVHXXXXXXXXXXXNVQHSIPRQRTCSHCRQTGHTSNNCP-XXXXX 427
            QL E CGTGSR    +            N Q S  RQ  C HC QTGH+SN+CP      
Sbjct: 723  QLTEICGTGSR----ISARGRGSNASSTNAQRSNTRQTACIHCSQTGHSSNDCPSRVPRS 778

Query: 426  XXXXXXXXXXXXGEPSIPCGSCGSPCMLRTANTENNRGKKFYSCQG--CNFFIWEDVXXX 253
                        GE SIPC  CG+PC+LRTANTENNRG+KFYSCQ   CNFF+WED    
Sbjct: 779  RNSQSHRTNMPSGESSIPCNVCGTPCILRTANTENNRGRKFYSCQSQECNFFVWEDSLNS 838

Query: 252  XXXXXXXXXXXATAQXXXXXXXXXXXXXXXXXXXXXGSNANGGNFVSATGEQITDRRCFI 73
                       A +                      G+  +   FVSATG+ ++ RRCF+
Sbjct: 839  SSGGRSAQPATARSSASNPSRRGGRGRGDRGRGRQSGARTSDTTFVSATGDPVSGRRCFV 898

Query: 72   CQDPSHFANVCPSRGM 25
            C DPSHFANVCP+RGM
Sbjct: 899  CGDPSHFANVCPNRGM 914


>ref|XP_002307047.1| predicted protein [Populus trichocarpa] gi|222856496|gb|EEE94043.1|
            predicted protein [Populus trichocarpa]
          Length = 910

 Score =  900 bits (2327), Expect(2) = 0.0
 Identities = 428/554 (77%), Positives = 484/554 (87%), Gaps = 1/554 (0%)
 Frame = -1

Query: 2440 WLILWLDCDREGENIAFEVVQVCTEANRQLNVWRARFSSIIEREIHQSVQRLGRPNQLFS 2261
            WL+LWLDCDREGENIAFEV++VC   NR L + RARFS++IER+IH++ Q L  PNQ FS
Sbjct: 110  WLVLWLDCDREGENIAFEVMEVCKGVNRNLTIRRARFSALIERDIHEAAQNLIAPNQWFS 169

Query: 2260 DAVDVRQEIDLRIGASFTRFQTMLLRDAFVLDFAADGRNVVLSYGPCQFPTLGFVVERYW 2081
            DAVD RQEIDLRIGASFTRFQTMLLRD FV+D A D RN+VLSYGPCQFPTLGFVVERYW
Sbjct: 170  DAVDARQEIDLRIGASFTRFQTMLLRDRFVIDSAQDDRNLVLSYGPCQFPTLGFVVERYW 229

Query: 2080 EIQSHEPEEFWAINCTHNSDNCTASLNWMRGHLFDYTCAVIIYEMCIEEPTATVTNVR-Q 1904
            EIQSHEPEEFW INCTH SD   AS NWMRGHLFDYT +VI+YEMC+EEPTATV+N   Q
Sbjct: 230  EIQSHEPEEFWTINCTHRSDEGLASFNWMRGHLFDYTSSVILYEMCVEEPTATVSNRNHQ 289

Query: 1903 RETLKRPPHPLSTIELEKRASRYFRMGAKQTMKIAEELYLSGFISYPRTETDSFSSQADL 1724
            +E LK PP+PLSTIELEKRASRYFRM ++QTMK+AE+LY +GFISYPRTETDSFSS+ DL
Sbjct: 290  QEKLKYPPYPLSTIELEKRASRYFRMSSEQTMKVAEDLYQAGFISYPRTETDSFSSRTDL 349

Query: 1723 HTVVRDQQQHPVWGSYAQRLLDPEAGLWKNPSNGGHDDKAHPPIHPTKFSSGESNWTPDH 1544
            HT+V++ Q+HPVWGSYAQRLLDP AGLW+NPSNGGHDDKAHPPIHPTKFS+GES W+ DH
Sbjct: 350  HTIVQEHQEHPVWGSYAQRLLDPGAGLWRNPSNGGHDDKAHPPIHPTKFSAGESRWSQDH 409

Query: 1543 HKLYELVVRHFLACVSQPAVGAETTVDIDIAGELFCTTGRVILAKNYLEVYRFESWGGSI 1364
            H+LYELVVRHFLACVSQPAVGAET V+IDIAGE F  +GRVILAKNYL+VYRFESWG S+
Sbjct: 410  HRLYELVVRHFLACVSQPAVGAETVVEIDIAGERFSASGRVILAKNYLDVYRFESWGDSM 469

Query: 1363 IPTYTLGQQFIPTTLTLDSGVTRPPPLLSEADLMECMDKAGIGTDATMHDHIQKLLDRYY 1184
            IPTY  GQQFIP TLTLDSGVTRPPPLLSEADL+ CMDKAGIGTDATMHDHI+KLLDR+Y
Sbjct: 470  IPTYLQGQQFIPATLTLDSGVTRPPPLLSEADLLSCMDKAGIGTDATMHDHIKKLLDRFY 529

Query: 1183 AIKDLNTTRFTPTNLGEALVMGYDDMGYELWKPYLRAMMESDMKAVSVGTKNKAEVLATC 1004
              KD N TRF+PTNLGEALVMGYDDMGYELWKP LR++ME DMK VSVG K+KAEVLATC
Sbjct: 530  VTKDAN-TRFSPTNLGEALVMGYDDMGYELWKPNLRSLMECDMKEVSVGNKSKAEVLATC 588

Query: 1003 LEQMKACFVDARLKKVKLFEAMDVFFERSNRPVTNEQHTFGDVVRRCDRCRESDMVLKRK 824
            L+QMKACF+DA++ KVKL EAM +FF RS+R   ++    G+ VR C  C+E++MVLK+ 
Sbjct: 589  LQQMKACFLDAKVNKVKLLEAMAIFFNRSDRSNGDDSSALGENVRPCGLCQEANMVLKKN 648

Query: 823  PDGNFMVGCLGYPQ 782
             DGNFMVGC G+PQ
Sbjct: 649  RDGNFMVGCSGFPQ 662



 Score =  214 bits (544), Expect(2) = 0.0
 Identities = 121/255 (47%), Positives = 137/255 (53%), Gaps = 3/255 (1%)
 Frame = -2

Query: 783  KNAVWLPGPISEAXXXXXXXXXXTPGPVLKIQFKFRRMEIPMHFDVEHLGCIGGCDDILK 604
            +NAVWLPGP+ EA          TPGPV  IQFKFR++EIP  F+V HLGCIGGCD+ L+
Sbjct: 664  RNAVWLPGPVLEATITNNICNSCTPGPVYLIQFKFRQLEIPPGFNVNHLGCIGGCDETLR 723

Query: 603  QLIETCGTGSRDILGVHXXXXXXXXXXXNVQHSIPRQRTCSHCRQTGHTSNNCP-XXXXX 427
            QLIE CGTGSR    V            N Q S  RQ  C +C QTGH S +CP      
Sbjct: 724  QLIEICGTGSR----VQGGNGPPTTTPSNPQRSNSRQAPCIYCYQTGHASTDCPSRISAT 779

Query: 426  XXXXXXXXXXXXGEPSIPCGSCGSPCMLRTANTENNRGKKFYSC--QGCNFFIWEDVXXX 253
                        GE SIPC +CG+PC+LRTANT NNRG+KFYSC  Q CNFF+WED    
Sbjct: 780  RHVQSHGMNQQNGESSIPCSTCGTPCVLRTANTANNRGRKFYSCSSQACNFFVWED---- 835

Query: 252  XXXXXXXXXXXATAQXXXXXXXXXXXXXXXXXXXXXGSNANGGNFVSATGEQITDRRCFI 73
                       A                           A    FVSATGE I+ RRCF+
Sbjct: 836  -NLNNGSAPRSAPRPNMSNSASNPSRRGGRGRGVQNAGRAADVTFVSATGEPISGRRCFV 894

Query: 72   CQDPSHFANVCPSRG 28
            C DPSHFAN CPSRG
Sbjct: 895  CGDPSHFANACPSRG 909


>ref|XP_004136599.1| PREDICTED: DNA topoisomerase 3-alpha-like [Cucumis sativus]
          Length = 925

 Score =  882 bits (2280), Expect(2) = 0.0
 Identities = 414/555 (74%), Positives = 479/555 (86%), Gaps = 1/555 (0%)
 Frame = -1

Query: 2443 QWLILWLDCDREGENIAFEVVQVCTEANRQLNVWRARFSSIIEREIHQSVQRLGRPNQLF 2264
            QWL+LWLDCDREGENIAFEVV+VCT  N  LN+ RA FS++IER+IH +VQ L +PN+ F
Sbjct: 115  QWLVLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIERDIHDAVQNLVQPNKWF 174

Query: 2263 SDAVDVRQEIDLRIGASFTRFQTMLLRDAFVLDFAADGRNVVLSYGPCQFPTLGFVVERY 2084
            +DAVD RQEIDLRIGASFTRFQT+LL+DAF +D   D RN+VLSYGPCQFPTLGFVVERY
Sbjct: 175  ADAVDARQEIDLRIGASFTRFQTLLLKDAFNIDSVTDDRNIVLSYGPCQFPTLGFVVERY 234

Query: 2083 WEIQSHEPEEFWAINCTHNSDNCTASLNWMRGHLFDYTCAVIIYEMCIEEPTATVTNVRQ 1904
            WEIQSHEPEEFW INC+HNSD+  ++ +W RG+LFDYTCAV+ YEMC++E TATV NVR+
Sbjct: 235  WEIQSHEPEEFWTINCSHNSDDGISTFSWTRGNLFDYTCAVVFYEMCVQESTATVVNVRR 294

Query: 1903 RETLKRPPHPLSTIELEKRASRYFRMGAKQTMKIAEELYLSGFISYPRTETDSFSSQADL 1724
            +ETLK PPHPL+TIELEKRASRYFRM ++ TMK+AE+LY +GFISYPRTETD FS + DL
Sbjct: 295  QETLKYPPHPLNTIELEKRASRYFRMSSEHTMKVAEDLYQAGFISYPRTETDCFSQRTDL 354

Query: 1723 HTVVRDQQQHPVWGSYAQRLLDPEAGLWKNPSNGGHDDKAHPPIHPTKFSSGESNWTPDH 1544
            H +VR+QQ+H +WG+YAQRLLDPE+ LW+NPS GGHDDKAHPPIHPTKFS+GE  W+ DH
Sbjct: 355  HGIVREQQEHQIWGAYAQRLLDPESRLWRNPSGGGHDDKAHPPIHPTKFSAGERGWSQDH 414

Query: 1543 HKLYELVVRHFLACVSQPAVGAETTVDIDIAGELFCTTGRV-ILAKNYLEVYRFESWGGS 1367
            H+LYELVVRHFLACVSQPAVGA TTV+IDIAGELF T+ R   + +NYL+VYRFESWGGS
Sbjct: 415  HRLYELVVRHFLACVSQPAVGAVTTVEIDIAGELFSTSKRANSILRNYLDVYRFESWGGS 474

Query: 1366 IIPTYTLGQQFIPTTLTLDSGVTRPPPLLSEADLMECMDKAGIGTDATMHDHIQKLLDRY 1187
             IPTY  GQQF+PTTLTLDSGVTRPPPLLSEADL+ CMDK GIGTDATMHDHI+KLLDR+
Sbjct: 475  TIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEADLLSCMDKEGIGTDATMHDHIKKLLDRF 534

Query: 1186 YAIKDLNTTRFTPTNLGEALVMGYDDMGYELWKPYLRAMMESDMKAVSVGTKNKAEVLAT 1007
            YA KD N TRFTPTNLGEALVMGYDDMGY LWKP LRA+ME +MKAVS GTK K EVLA+
Sbjct: 535  YATKDAN-TRFTPTNLGEALVMGYDDMGYNLWKPNLRAIMEHEMKAVSEGTKRKDEVLAS 593

Query: 1006 CLEQMKACFVDARLKKVKLFEAMDVFFERSNRPVTNEQHTFGDVVRRCDRCRESDMVLKR 827
            CL+QMK CF+DAR+ K KL EAM+VFFERSN    +E    G+VV+ C  C++SDMVLKR
Sbjct: 594  CLQQMKTCFLDARVNKQKLLEAMEVFFERSNGSGGDENRAVGEVVQLCGLCQDSDMVLKR 653

Query: 826  KPDGNFMVGCLGYPQ 782
              DGN+MVGCLG+PQ
Sbjct: 654  NRDGNYMVGCLGFPQ 668



 Score =  208 bits (530), Expect(2) = 0.0
 Identities = 117/264 (44%), Positives = 136/264 (51%), Gaps = 12/264 (4%)
 Frame = -2

Query: 783  KNAVWLPGPISEAXXXXXXXXXXTPGPVLKIQFKFRRMEIPMHFDVEHLGCIGGCDDILK 604
            +N VWLPG I+EA          TPGPV  IQFKFR++EIP +++  HLGC+GGCDDIL+
Sbjct: 670  RNVVWLPGSIAEATVTSDRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILR 729

Query: 603  QLIETCGTGSRDILGVHXXXXXXXXXXXNVQHSIPRQRTCSHCRQTGHTSNNCPXXXXXX 424
            QL E CGTGSR                 NVQ   P Q  C  C+Q GH+SN+CP      
Sbjct: 730  QLTEICGTGSR------VSARGRGTSSSNVQRDNPGQSVCIFCQQVGHSSNDCP---SQS 780

Query: 423  XXXXXXXXXXXGEPSIPCGSCGSPCMLRTANTENNRGKKFYSCQG--CNFFIWED----- 265
                       GE S+ C SCGSPC+LRTANT NNRG+KFY CQ   CNFF+WED     
Sbjct: 781  VGSRSAQRRQSGESSVSCSSCGSPCVLRTANTANNRGRKFYKCQSQECNFFVWEDNVDNG 840

Query: 264  -----VXXXXXXXXXXXXXXATAQXXXXXXXXXXXXXXXXXXXXXGSNANGGNFVSATGE 100
                 V                                       G       FVSATGE
Sbjct: 841  NGGNSVQRSNVRSSGSAPSNLNPGQNTGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGE 900

Query: 99   QITDRRCFICQDPSHFANVCPSRG 28
             I+DRRCF+C DPSHFANVCP+RG
Sbjct: 901  PISDRRCFVCGDPSHFANVCPNRG 924


>ref|XP_002527391.1| prokaryotic DNA topoisomerase, putative [Ricinus communis]
            gi|223533243|gb|EEF34998.1| prokaryotic DNA
            topoisomerase, putative [Ricinus communis]
          Length = 833

 Score =  905 bits (2340), Expect(2) = 0.0
 Identities = 429/554 (77%), Positives = 483/554 (87%)
 Frame = -1

Query: 2443 QWLILWLDCDREGENIAFEVVQVCTEANRQLNVWRARFSSIIEREIHQSVQRLGRPNQLF 2264
            QWLILWLDCDREGENIAFEVV VC  ANR L V RARFS++I+REIH++ Q L  PNQ F
Sbjct: 112  QWLILWLDCDREGENIAFEVVDVCRSANRYLTVRRARFSALIDREIHEAAQNLVSPNQWF 171

Query: 2263 SDAVDVRQEIDLRIGASFTRFQTMLLRDAFVLDFAADGRNVVLSYGPCQFPTLGFVVERY 2084
            SDAVD RQEIDLRIGASFTRFQT+LLRD FV+D A D RN+VLSYGPCQFPTLGF+VERY
Sbjct: 172  SDAVDARQEIDLRIGASFTRFQTLLLRDKFVIDSAQDDRNLVLSYGPCQFPTLGFIVERY 231

Query: 2083 WEIQSHEPEEFWAINCTHNSDNCTASLNWMRGHLFDYTCAVIIYEMCIEEPTATVTNVRQ 1904
            WEIQSHE EEFW INC+H S    A+ +WMRGHLFDYT AVI+YE+C+ EPTATVT V+Q
Sbjct: 232  WEIQSHESEEFWTINCSHTSVEGIANFSWMRGHLFDYTSAVILYELCVLEPTATVTKVQQ 291

Query: 1903 RETLKRPPHPLSTIELEKRASRYFRMGAKQTMKIAEELYLSGFISYPRTETDSFSSQADL 1724
            +E  K PP+PLSTIELEKRASRYF M ++QTMK+AE+LY SGFISYPRTETD FSS+ DL
Sbjct: 292  KEKQKYPPYPLSTIELEKRASRYFHMSSEQTMKVAEDLYQSGFISYPRTETDGFSSRTDL 351

Query: 1723 HTVVRDQQQHPVWGSYAQRLLDPEAGLWKNPSNGGHDDKAHPPIHPTKFSSGESNWTPDH 1544
            HT+VRDQQ HP+WGSYAQRLLDP  GLW+NPS GGHDDKAHPPIHPTKFS GE+ W+ DH
Sbjct: 352  HTIVRDQQAHPIWGSYAQRLLDPGTGLWRNPSTGGHDDKAHPPIHPTKFSPGETGWSQDH 411

Query: 1543 HKLYELVVRHFLACVSQPAVGAETTVDIDIAGELFCTTGRVILAKNYLEVYRFESWGGSI 1364
            H++YELVVRHFLACVSQPAVGAETTV+IDIAGE F  +GRVILAKNYL+VYRFESWGGS+
Sbjct: 412  HRVYELVVRHFLACVSQPAVGAETTVEIDIAGEQFSASGRVILAKNYLDVYRFESWGGSM 471

Query: 1363 IPTYTLGQQFIPTTLTLDSGVTRPPPLLSEADLMECMDKAGIGTDATMHDHIQKLLDRYY 1184
            IPTY  GQQF+PTTLTLDSGVTRPPPLLSE+DL+ CMDKAGIGTDATMHDHI+KLLDR+Y
Sbjct: 472  IPTYVHGQQFMPTTLTLDSGVTRPPPLLSESDLLSCMDKAGIGTDATMHDHIKKLLDRFY 531

Query: 1183 AIKDLNTTRFTPTNLGEALVMGYDDMGYELWKPYLRAMMESDMKAVSVGTKNKAEVLATC 1004
            A KD N TRF+PTNLGEALVMGYDDMGYELWKP LR+MME DMK VS+G K+KAEVL TC
Sbjct: 532  ATKDAN-TRFSPTNLGEALVMGYDDMGYELWKPNLRSMMEYDMKEVSMGHKSKAEVLETC 590

Query: 1003 LEQMKACFVDARLKKVKLFEAMDVFFERSNRPVTNEQHTFGDVVRRCDRCRESDMVLKRK 824
            L+QMK CF+DAR+ KVKL EAM VFFERS+R   N+ H   D+VR+C  CRE++MVL+R 
Sbjct: 591  LQQMKTCFLDARINKVKLLEAMAVFFERSDRSSGNDNHATVDIVRQCGLCRETNMVLRRN 650

Query: 823  PDGNFMVGCLGYPQ 782
             DGNFMVGC+GYPQ
Sbjct: 651  RDGNFMVGCMGYPQ 664



 Score =  167 bits (422), Expect(2) = 0.0
 Identities = 90/172 (52%), Positives = 101/172 (58%), Gaps = 3/172 (1%)
 Frame = -2

Query: 783  KNAVWLPGPISEAXXXXXXXXXXTPGPVLKIQFKFRRMEIPMHFDVEHLGCIGGCDDILK 604
            +NA+WLPGPI EA          TPGPV  IQFKFR++EIP  F V HLGCIGGCDD L+
Sbjct: 666  RNAIWLPGPIIEATVTTSVCNYCTPGPVYLIQFKFRQLEIPPGFSVNHLGCIGGCDDTLR 725

Query: 603  QLIETCGTGSRDILGVHXXXXXXXXXXXNVQHSIPRQRTCSHCRQTGHTSNNCP-XXXXX 427
            QL + CGTGSR    V            N Q S  R  TC HC QTGH S +CP      
Sbjct: 726  QLTDICGTGSR----VQARARAPNTTPSNSQQSNTRFATCVHCHQTGHASIDCPLQLSGS 781

Query: 426  XXXXXXXXXXXXGEPSIPCGSCGSPCMLRTANTENNRGKKFYSC--QGCNFF 277
                        GE S+ CG+CG+PC LRTANT NNRG+KFYSC  Q CNFF
Sbjct: 782  RRVQSRGTNEQMGESSVTCGTCGTPCALRTANTANNRGRKFYSCPSQSCNFF 833


>ref|NP_201197.1| DNA topoisomerase III [Arabidopsis thaliana]
            gi|8777305|dbj|BAA96895.1| DNA topoisomerase III
            [Arabidopsis thaliana] gi|169218912|gb|ACA50279.1|
            topoisomerase 3 alpha [Arabidopsis thaliana]
            gi|332010433|gb|AED97816.1| DNA topoisomerase III
            [Arabidopsis thaliana]
          Length = 926

 Score =  867 bits (2241), Expect(2) = 0.0
 Identities = 411/554 (74%), Positives = 467/554 (84%), Gaps = 1/554 (0%)
 Frame = -1

Query: 2440 WLILWLDCDREGENIAFEVVQVCTEANRQLNVWRARFSSIIEREIHQSVQRLGRPNQLFS 2261
            WL+LWLDCDREGENIAFEVV VC      L + RA FS++I+R+IH++VQ L  PNQLF+
Sbjct: 117  WLVLWLDCDREGENIAFEVVDVCRAVKHNLFIRRAHFSALIDRDIHEAVQNLRDPNQLFA 176

Query: 2260 DAVDVRQEIDLRIGASFTRFQTMLLRDAFVLDFAADGRNVVLSYGPCQFPTLGFVVERYW 2081
            +AVD RQEIDLRIGASFTRFQTMLLRD F +D   + R+ V+SYGPCQFPTLGF+VERYW
Sbjct: 177  EAVDARQEIDLRIGASFTRFQTMLLRDRFAIDSTGEERSRVISYGPCQFPTLGFIVERYW 236

Query: 2080 EIQSHEPEEFWAINCTHNSDNCTASLNWMRGHLFDYTCAVIIYEMCIEEPTATVTNV-RQ 1904
            EIQ+HEPEEFW INC+H S+   A+ NWMRGHLFDY  AVI+YEMC+EEPTATV NV   
Sbjct: 237  EIQAHEPEEFWTINCSHQSEEGLATFNWMRGHLFDYASAVILYEMCVEEPTATVMNVPHP 296

Query: 1903 RETLKRPPHPLSTIELEKRASRYFRMGAKQTMKIAEELYLSGFISYPRTETDSFSSQADL 1724
            RE  K PP+PL+TIELEKRASRYFR+ ++ TMK+AEELY +GFISYPRTETDSFSS+ DL
Sbjct: 297  RERFKYPPYPLNTIELEKRASRYFRLSSEHTMKVAEELYQAGFISYPRTETDSFSSRTDL 356

Query: 1723 HTVVRDQQQHPVWGSYAQRLLDPEAGLWKNPSNGGHDDKAHPPIHPTKFSSGESNWTPDH 1544
              +V +Q +HP WGSYAQRLL+PE GLW+NP+NGGHDDKAHPPIHPTKFSSGESNW+ DH
Sbjct: 357  RAMVEEQTRHPAWGSYAQRLLEPEGGLWRNPANGGHDDKAHPPIHPTKFSSGESNWSRDH 416

Query: 1543 HKLYELVVRHFLACVSQPAVGAETTVDIDIAGELFCTTGRVILAKNYLEVYRFESWGGSI 1364
              +YELVVRH+LACVSQPAV AETTV+IDIAGE F  +GR ILAKNYLEVYRFESWGGS+
Sbjct: 417  LNVYELVVRHYLACVSQPAVAAETTVEIDIAGERFSASGRAILAKNYLEVYRFESWGGSV 476

Query: 1363 IPTYTLGQQFIPTTLTLDSGVTRPPPLLSEADLMECMDKAGIGTDATMHDHIQKLLDRYY 1184
            IP Y  GQQFIPTTLTLD+ VTRPPPLL EADL+ CMDKAGIGTDATMHDHI+KLLDR Y
Sbjct: 477  IPVYEKGQQFIPTTLTLDAAVTRPPPLLCEADLLSCMDKAGIGTDATMHDHIKKLLDRGY 536

Query: 1183 AIKDLNTTRFTPTNLGEALVMGYDDMGYELWKPYLRAMMESDMKAVSVGTKNKAEVLATC 1004
            A KD N TRF+PTNLGEALVMGYDDMGYELWKP LRA+ME DM  VSVG K KAEVL TC
Sbjct: 537  ATKDAN-TRFSPTNLGEALVMGYDDMGYELWKPNLRALMEHDMNEVSVGRKTKAEVLETC 595

Query: 1003 LEQMKACFVDARLKKVKLFEAMDVFFERSNRPVTNEQHTFGDVVRRCDRCRESDMVLKRK 824
            L+QMKACF+DAR+KK KL EAM +FFERSN    +E  T G+VVRRC+ C ESDM L++ 
Sbjct: 596  LQQMKACFLDARVKKSKLLEAMTIFFERSNNTDESESQTAGEVVRRCNLCNESDMALRKN 655

Query: 823  PDGNFMVGCLGYPQ 782
             DGNFMVGC+ YPQ
Sbjct: 656  RDGNFMVGCMNYPQ 669



 Score =  196 bits (498), Expect(2) = 0.0
 Identities = 111/255 (43%), Positives = 133/255 (52%), Gaps = 4/255 (1%)
 Frame = -2

Query: 783  KNAVWLPGPISEAXXXXXXXXXXTPGPVLKIQFKFRRMEIPMHFDVEHLGCIGGCDDILK 604
            +NAVWLPGP  EA           PGPV KI FKFR++ IP  FDV HLGC+GGCDDILK
Sbjct: 671  RNAVWLPGPTLEASVTTNVCQSCGPGPVYKILFKFRQIGIPPGFDVNHLGCVGGCDDILK 730

Query: 603  QLIETCGTGSRDILGVHXXXXXXXXXXXNVQHSIPRQ-RTCSHCRQTGHTSNNCP-XXXX 430
            QLI+ CGTGSR                 N+Q S  RQ   C HC+Q GH S NCP     
Sbjct: 731  QLIDICGTGSRS----QARRTPGTAPSNNIQGSNTRQSNVCIHCQQRGHASTNCPSRVPA 786

Query: 429  XXXXXXXXXXXXXGEPSIPCGSCGSPCMLRTANTENNRGKKFYSC--QGCNFFIWEDVXX 256
                          E ++ C +CGS C+LRTANTE NRG++F+SC  QGC+FF WED   
Sbjct: 787  SRNSRPTATNPRNDESTVSCNTCGSQCVLRTANTEANRGRQFFSCPTQGCSFFAWED--- 843

Query: 255  XXXXXXXXXXXXATAQXXXXXXXXXXXXXXXXXXXXXGSNANGGNFVSATGEQITDRRCF 76
                        + +                      G   +G +FVSATGE ++  RCF
Sbjct: 844  SINNSSGNATTGSNSGGSGRRGSRGRGRGGRGGQSSGGRRGSGTSFVSATGEPVSGIRCF 903

Query: 75   ICQDPSHFANVCPSR 31
             C DPSHFAN CP+R
Sbjct: 904  SCGDPSHFANACPNR 918