BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00016294
         (1081 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002330745.1| predicted protein [Populus trichocarpa] gi|2...   475   e-132
emb|CBI19390.3| unnamed protein product [Vitis vinifera]              473   e-131
ref|XP_002283369.1| PREDICTED: chitobiosyldiphosphodolichol beta...   473   e-131
gb|AFK48851.1| unknown [Medicago truncatula]                          470   e-130
ref|XP_003596188.1| Chitobiosyldiphosphodolichol beta-mannosyltr...   470   e-130

>ref|XP_002330745.1| predicted protein [Populus trichocarpa] gi|222872521|gb|EEF09652.1|
            predicted protein [Populus trichocarpa]
          Length = 481

 Score =  475 bits (1223), Expect = e-132
 Identities = 228/300 (76%), Positives = 259/300 (86%), Gaps = 3/300 (1%)
 Frame = +3

Query: 162  IGRRGRAAVVVLGDIGRSPRMQYHALSLARQACLEVDIVAYGGSDPHSAVLEHKSIHIHE 341
            +G+RGRA VVVLGDIGRSPRMQYHALSLARQA L+VDIVAYGGS+PH A+ E++SIHIH+
Sbjct: 1    MGKRGRACVVVLGDIGRSPRMQYHALSLARQASLQVDIVAYGGSEPHMALRENQSIHIHK 60

Query: 342  MNQWPPIPRSLPKILRPLVLLIKPVVQFLMLLWFLCVKIPPPDVFIVQNPPSVPTLVAVK 521
            M QWP IP+ LPK+ +P +LL+KP+ QFLMLLW+LCVKIPPPDVF+VQNPPSVPTLVAVK
Sbjct: 61   MKQWPAIPQGLPKMFKPFMLLLKPLFQFLMLLWYLCVKIPPPDVFLVQNPPSVPTLVAVK 120

Query: 522  WASWLRHSKFIVDWHNFGYTLLALSLGRSSPFVAVYRWFEKHYGKMADGSLCVTQAMQLE 701
            WASWLR++KFIVDWHNFGYTLLALSLGR+S FV VYRWFE+HYGKMA GSLCVT+AMQ E
Sbjct: 121  WASWLRNAKFIVDWHNFGYTLLALSLGRNSRFVTVYRWFERHYGKMAHGSLCVTRAMQHE 180

Query: 702  LAQNWGIEAIVLYDQPPEFFHPTSLDEKHKLFCRIDKNLKEPYGLQDCISNGKDG-HDPN 878
            L QNWGI+A VLYDQPPEFFHP SL+EKHKLFCR+ K++ E  G+ DC S+G  G   PN
Sbjct: 181  LTQNWGIKAAVLYDQPPEFFHPASLEEKHKLFCRLGKHISESQGVHDCASHGAVGMGSPN 240

Query: 879  AN--LFTIQVGTDINLNQNNPALIVSSTSWTPDEDFNILLEAALMYDRRVAALLNEDDST 1052
             N  LFT  V  DI L  N PAL+VSSTSWT DEDF ILLEAA+MYDRRVAA+LNEDDST
Sbjct: 241  LNETLFTTMVADDIFLKPNRPALVVSSTSWTADEDFGILLEAAVMYDRRVAAILNEDDST 300


>emb|CBI19390.3| unnamed protein product [Vitis vinifera]
          Length = 484

 Score =  473 bits (1218), Expect = e-131
 Identities = 233/314 (74%), Positives = 261/314 (83%), Gaps = 3/314 (0%)
 Frame = +3

Query: 144  GKSKASIGRRGRAAVVVLGDIGRSPRMQYHALSLARQACLEVDIVAYGGSDPHSAVLEHK 323
            G+ + +IGRRGRAAVVVLGDIGRSPRMQYHALSLARQA LEVDIVAYGGS PHSAVLE+ 
Sbjct: 3    GRKEENIGRRGRAAVVVLGDIGRSPRMQYHALSLARQASLEVDIVAYGGSTPHSAVLENP 62

Query: 324  SIHIHEMNQWPPIPRSLPKILRPLVLLIKPVVQFLMLLWFLCVKIPPPDVFIVQNPPSVP 503
            SIHIH M QWP IPR + KI  PLVLL K + QF  L WFLCVKIP PDVFIVQNPPSVP
Sbjct: 63   SIHIHTMKQWPTIPRIVSKIFYPLVLLFKALFQFFTLFWFLCVKIPSPDVFIVQNPPSVP 122

Query: 504  TLVAVKWASWLRHSKFIVDWHNFGYTLLALSLGRSSPFVAVYRWFEKHYGKMADGSLCVT 683
            TLVAVKWASWLR+S FIVDWHNFGYTLL LSLGRSS FVA+Y WFEK+YGK A+GSLCVT
Sbjct: 123  TLVAVKWASWLRNSAFIVDWHNFGYTLLGLSLGRSSRFVALYHWFEKYYGKAANGSLCVT 182

Query: 684  QAMQLELAQNWGIEAIVLYDQPPEFFHPTSLDEKHKLFCRIDKNLKEPYGLQDCISNGKD 863
            +AMQ ELAQNWGI+A VLYDQPPEFF PT ++EKHKLFCR+ K+L  P G QDC++ G  
Sbjct: 183  RAMQHELAQNWGIKATVLYDQPPEFFRPTPMEEKHKLFCRLHKDLCHPRGGQDCVTAGTM 242

Query: 864  ---GHDPNANLFTIQVGTDINLNQNNPALIVSSTSWTPDEDFNILLEAALMYDRRVAALL 1034
                 D +  LFT ++ TDI L  N PAL+VSSTSWTPDEDF +LLEAA+MYDRRVAA+L
Sbjct: 243  ELWNQDTDETLFTAKMDTDIFLKSNRPALVVSSTSWTPDEDFGMLLEAAVMYDRRVAAIL 302

Query: 1035 NEDDSTPDDVLSRE 1076
            NEDDST ++VL +E
Sbjct: 303  NEDDSTKEEVLWKE 316


>ref|XP_002283369.1| PREDICTED: chitobiosyldiphosphodolichol beta-mannosyltransferase-like
            [Vitis vinifera]
          Length = 598

 Score =  473 bits (1218), Expect = e-131
 Identities = 233/314 (74%), Positives = 261/314 (83%), Gaps = 3/314 (0%)
 Frame = +3

Query: 144  GKSKASIGRRGRAAVVVLGDIGRSPRMQYHALSLARQACLEVDIVAYGGSDPHSAVLEHK 323
            G+ + +IGRRGRAAVVVLGDIGRSPRMQYHALSLARQA LEVDIVAYGGS PHSAVLE+ 
Sbjct: 118  GRKEENIGRRGRAAVVVLGDIGRSPRMQYHALSLARQASLEVDIVAYGGSTPHSAVLENP 177

Query: 324  SIHIHEMNQWPPIPRSLPKILRPLVLLIKPVVQFLMLLWFLCVKIPPPDVFIVQNPPSVP 503
            SIHIH M QWP IPR + KI  PLVLL K + QF  L WFLCVKIP PDVFIVQNPPSVP
Sbjct: 178  SIHIHTMKQWPTIPRIVSKIFYPLVLLFKALFQFFTLFWFLCVKIPSPDVFIVQNPPSVP 237

Query: 504  TLVAVKWASWLRHSKFIVDWHNFGYTLLALSLGRSSPFVAVYRWFEKHYGKMADGSLCVT 683
            TLVAVKWASWLR+S FIVDWHNFGYTLL LSLGRSS FVA+Y WFEK+YGK A+GSLCVT
Sbjct: 238  TLVAVKWASWLRNSAFIVDWHNFGYTLLGLSLGRSSRFVALYHWFEKYYGKAANGSLCVT 297

Query: 684  QAMQLELAQNWGIEAIVLYDQPPEFFHPTSLDEKHKLFCRIDKNLKEPYGLQDCISNGKD 863
            +AMQ ELAQNWGI+A VLYDQPPEFF PT ++EKHKLFCR+ K+L  P G QDC++ G  
Sbjct: 298  RAMQHELAQNWGIKATVLYDQPPEFFRPTPMEEKHKLFCRLHKDLCHPRGGQDCVTAGTM 357

Query: 864  ---GHDPNANLFTIQVGTDINLNQNNPALIVSSTSWTPDEDFNILLEAALMYDRRVAALL 1034
                 D +  LFT ++ TDI L  N PAL+VSSTSWTPDEDF +LLEAA+MYDRRVAA+L
Sbjct: 358  ELWNQDTDETLFTAKMDTDIFLKSNRPALVVSSTSWTPDEDFGMLLEAAVMYDRRVAAIL 417

Query: 1035 NEDDSTPDDVLSRE 1076
            NEDDST ++VL +E
Sbjct: 418  NEDDSTKEEVLWKE 431


>gb|AFK48851.1| unknown [Medicago truncatula]
          Length = 484

 Score =  470 bits (1209), Expect = e-130
 Identities = 221/316 (69%), Positives = 267/316 (84%), Gaps = 5/316 (1%)
 Frame = +3

Query: 144  GKSKASI--GRRGRAAVVVLGDIGRSPRMQYHALSLARQACLEVDIVAYGGSDPHSAVLE 317
            GKSK  +  GRRGRA VVVLGDIGRSPRMQYHALSL+ QA LEVDIVAYGGS+PH+++L 
Sbjct: 3    GKSKEKMEGGRRGRACVVVLGDIGRSPRMQYHALSLSNQAFLEVDIVAYGGSEPHTSLLA 62

Query: 318  HKSIHIHEMNQWPPIPRSLPKILRPLVLLIKPVVQFLMLLWFLCVKIPPPDVFIVQNPPS 497
            + SIHIH M QWP   ++LPKIL+P +LL+KP++QF+MLLW+LCVKIP PD+FIVQNPPS
Sbjct: 63   NPSIHIHLMKQWPTARQNLPKILQPFMLLLKPLIQFVMLLWYLCVKIPSPDIFIVQNPPS 122

Query: 498  VPTLVAVKWASWLRHSKFIVDWHNFGYTLLALSLGRSSPFVAVYRWFEKHYGKMADGSLC 677
            VPTLVAVKWASWLR+S F++DWHNFGYTLL +SLGR+S FV++Y+WFEKHYGKMAD SLC
Sbjct: 123  VPTLVAVKWASWLRNSSFVIDWHNFGYTLLGMSLGRNSRFVSLYKWFEKHYGKMADTSLC 182

Query: 678  VTQAMQLELAQNWGIEAIVLYDQPPEFFHPTSLDEKHKLFCRIDKNLKEPYGLQDCISNG 857
            VT+AMQ ELAQNWGI A VLYDQPP+FFHP SL+E+HKLFCR++++   P G+ DC+SNG
Sbjct: 183  VTKAMQHELAQNWGINATVLYDQPPDFFHPASLEERHKLFCRLNEHFYHPLGVGDCVSNG 242

Query: 858  ---KDGHDPNANLFTIQVGTDINLNQNNPALIVSSTSWTPDEDFNILLEAALMYDRRVAA 1028
                     N  LFT + G++++L  N PAL+VSSTSWTPDEDF ILLEAALMYDRRVAA
Sbjct: 243  TSLMSSQIQNETLFTTEAGSNVSLKPNRPALVVSSTSWTPDEDFGILLEAALMYDRRVAA 302

Query: 1029 LLNEDDSTPDDVLSRE 1076
            +LNEDDS  ++V+ +E
Sbjct: 303  ILNEDDSLDEEVIWKE 318


>ref|XP_003596188.1| Chitobiosyldiphosphodolichol beta-mannosyltransferase [Medicago
            truncatula] gi|355485236|gb|AES66439.1|
            Chitobiosyldiphosphodolichol beta-mannosyltransferase
            [Medicago truncatula]
          Length = 498

 Score =  470 bits (1209), Expect = e-130
 Identities = 221/316 (69%), Positives = 267/316 (84%), Gaps = 5/316 (1%)
 Frame = +3

Query: 144  GKSKASI--GRRGRAAVVVLGDIGRSPRMQYHALSLARQACLEVDIVAYGGSDPHSAVLE 317
            GKSK  +  GRRGRA VVVLGDIGRSPRMQYHALSL+ QA LEVDIVAYGGS+PH+++L 
Sbjct: 3    GKSKEKMEGGRRGRACVVVLGDIGRSPRMQYHALSLSNQAFLEVDIVAYGGSEPHTSLLA 62

Query: 318  HKSIHIHEMNQWPPIPRSLPKILRPLVLLIKPVVQFLMLLWFLCVKIPPPDVFIVQNPPS 497
            + SIHIH M QWP   ++LPKIL+P +LL+KP++QF+MLLW+LCVKIP PD+FIVQNPPS
Sbjct: 63   NPSIHIHLMKQWPTARQNLPKILQPFMLLLKPLIQFVMLLWYLCVKIPSPDIFIVQNPPS 122

Query: 498  VPTLVAVKWASWLRHSKFIVDWHNFGYTLLALSLGRSSPFVAVYRWFEKHYGKMADGSLC 677
            VPTLVAVKWASWLR+S F++DWHNFGYTLL +SLGR+S FV++Y+WFEKHYGKMAD SLC
Sbjct: 123  VPTLVAVKWASWLRNSSFVIDWHNFGYTLLGMSLGRNSRFVSLYKWFEKHYGKMADTSLC 182

Query: 678  VTQAMQLELAQNWGIEAIVLYDQPPEFFHPTSLDEKHKLFCRIDKNLKEPYGLQDCISNG 857
            VT+AMQ ELAQNWGI A VLYDQPP+FFHP SL+E+HKLFCR++++   P G+ DC+SNG
Sbjct: 183  VTKAMQHELAQNWGINATVLYDQPPDFFHPASLEERHKLFCRLNEHFYHPLGVGDCVSNG 242

Query: 858  ---KDGHDPNANLFTIQVGTDINLNQNNPALIVSSTSWTPDEDFNILLEAALMYDRRVAA 1028
                     N  LFT + G++++L  N PAL+VSSTSWTPDEDF ILLEAALMYDRRVAA
Sbjct: 243  TSLMSSQIQNETLFTTEAGSNVSLKPNRPALVVSSTSWTPDEDFGILLEAALMYDRRVAA 302

Query: 1029 LLNEDDSTPDDVLSRE 1076
            +LNEDDS  ++V+ +E
Sbjct: 303  ILNEDDSLDEEVIWKE 318