BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00016240
         (1798 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002273941.2| PREDICTED: putative E3 ubiquitin-protein lig...   426   e-116
emb|CBI30594.3| unnamed protein product [Vitis vinifera]              426   e-116
ref|XP_002305782.1| predicted protein [Populus trichocarpa] gi|2...   387   e-105
emb|CBI21880.3| unnamed protein product [Vitis vinifera]              380   e-103
ref|XP_002274885.2| PREDICTED: putative E3 ubiquitin-protein lig...   375   e-101

>ref|XP_002273941.2| PREDICTED: putative E3 ubiquitin-protein ligase RING1a-like [Vitis
            vinifera]
          Length = 534

 Score =  426 bits (1095), Expect = e-116
 Identities = 237/442 (53%), Positives = 285/442 (64%), Gaps = 2/442 (0%)
 Frame = +2

Query: 50   TMPAQKRPLDTRTXXXXXXXXXXXXXXVAEVQQXXXXXXXXXXIGDKDEFITVALADIRK 229
            +MPAQKR  D                   +              G+KDEF+ V LA+IRK
Sbjct: 101  SMPAQKRAFDAMEGDPLQRHHGNHHEPPEDGDGYESDRSPSWSNGEKDEFVIVKLAEIRK 160

Query: 230  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 409
            EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD
Sbjct: 161  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 220

Query: 410  ALISALYPDIDKYXXXXXXXXXXXRARNKQIQASIAQTLRRQSEALVXXXXXXXXXXXXX 589
            ALI+ALYPDIDKY           + RNKQIQASIAQTLRRQSEAL              
Sbjct: 221  ALIAALYPDIDKYEQEELAFHEEEKTRNKQIQASIAQTLRRQSEALGRKRTTAKATAAAF 280

Query: 590  XXXPQVXXXXXXXXXXXXXEHQESDEEDNANDHDRAKDSSSADEQVTEVKPKRNRRWGGF 769
                Q                   D+ED AN +D  KDSSSA++   EVKPKR+RRWGG 
Sbjct: 281  ARRSQGNYRRGRRNYRATDLLGSEDDED-ANGNDGGKDSSSAEDHSAEVKPKRSRRWGGV 339

Query: 770  RXXXXXXXXXXXXXXXXXXX-EVNRDSLGASAGLVGNSEMLAWGRGGTRSNTRHGSVNAG 946
            R                    E NR+++GASAGLVGNSE+LAWG+GG RS+TR+GSV+ G
Sbjct: 340  RFSQPSSGAANADGGGDENDLEPNREAMGASAGLVGNSEILAWGKGGMRSHTRYGSVSGG 399

Query: 947  NGKQARNMRLSKLIDCIQNTVQN-AQLNIHLILVTLDKQRIPSLQCPHIRCSPTMSIRNL 1123
            NGK AR+ RLSKL+D ++N  QN  +L+IHL+LV+LD+QRIPSLQ P++ C PT+S+R+L
Sbjct: 400  NGKNARSSRLSKLVDYLRNLDQNDDELDIHLMLVSLDEQRIPSLQTPYLCCRPTLSVRHL 459

Query: 1124 CQFVGLKTSVEADDVEILLVKGLHSEADTTLTSSVSISSRTIDPCKDELHILEEHETLAE 1303
            CQ+V L+T + AD+VEIL++K   S+ + + +SS        DPC D L +LE  ETLA 
Sbjct: 460  CQYVALQTPLLADEVEILVIKEQLSKLNPSNSSSP-------DPCSDRLQVLEAQETLAV 512

Query: 1304 LQAMYSVNGRELLFAYRQKLSA 1369
            L+ M + N   LL AYR+KLSA
Sbjct: 513  LKTMRNANQSHLLLAYRRKLSA 534


>emb|CBI30594.3| unnamed protein product [Vitis vinifera]
          Length = 433

 Score =  426 bits (1094), Expect = e-116
 Identities = 237/441 (53%), Positives = 284/441 (64%), Gaps = 2/441 (0%)
 Frame = +2

Query: 53   MPAQKRPLDTRTXXXXXXXXXXXXXXVAEVQQXXXXXXXXXXIGDKDEFITVALADIRKE 232
            MPAQKR  D                   +              G+KDEF+ V LA+IRKE
Sbjct: 1    MPAQKRAFDAMEGDPLQRHHGNHHEPPEDGDGYESDRSPSWSNGEKDEFVIVKLAEIRKE 60

Query: 233  VQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDA 412
            VQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDA
Sbjct: 61   VQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDA 120

Query: 413  LISALYPDIDKYXXXXXXXXXXXRARNKQIQASIAQTLRRQSEALVXXXXXXXXXXXXXX 592
            LI+ALYPDIDKY           + RNKQIQASIAQTLRRQSEAL               
Sbjct: 121  LIAALYPDIDKYEQEELAFHEEEKTRNKQIQASIAQTLRRQSEALGRKRTTAKATAAAFA 180

Query: 593  XXPQVXXXXXXXXXXXXXEHQESDEEDNANDHDRAKDSSSADEQVTEVKPKRNRRWGGFR 772
               Q                   D+ED AN +D  KDSSSA++   EVKPKR+RRWGG R
Sbjct: 181  RRSQGNYRRGRRNYRATDLLGSEDDED-ANGNDGGKDSSSAEDHSAEVKPKRSRRWGGVR 239

Query: 773  XXXXXXXXXXXXXXXXXXX-EVNRDSLGASAGLVGNSEMLAWGRGGTRSNTRHGSVNAGN 949
                                E NR+++GASAGLVGNSE+LAWG+GG RS+TR+GSV+ GN
Sbjct: 240  FSQPSSGAANADGGGDENDLEPNREAMGASAGLVGNSEILAWGKGGMRSHTRYGSVSGGN 299

Query: 950  GKQARNMRLSKLIDCIQNTVQN-AQLNIHLILVTLDKQRIPSLQCPHIRCSPTMSIRNLC 1126
            GK AR+ RLSKL+D ++N  QN  +L+IHL+LV+LD+QRIPSLQ P++ C PT+S+R+LC
Sbjct: 300  GKNARSSRLSKLVDYLRNLDQNDDELDIHLMLVSLDEQRIPSLQTPYLCCRPTLSVRHLC 359

Query: 1127 QFVGLKTSVEADDVEILLVKGLHSEADTTLTSSVSISSRTIDPCKDELHILEEHETLAEL 1306
            Q+V L+T + AD+VEIL++K   S+ + + +SS        DPC D L +LE  ETLA L
Sbjct: 360  QYVALQTPLLADEVEILVIKEQLSKLNPSNSSSP-------DPCSDRLQVLEAQETLAVL 412

Query: 1307 QAMYSVNGRELLFAYRQKLSA 1369
            + M + N   LL AYR+KLSA
Sbjct: 413  KTMRNANQSHLLLAYRRKLSA 433


>ref|XP_002305782.1| predicted protein [Populus trichocarpa] gi|222848746|gb|EEE86293.1|
            predicted protein [Populus trichocarpa]
          Length = 463

 Score =  387 bits (994), Expect = e-105
 Identities = 211/397 (53%), Positives = 266/397 (67%), Gaps = 5/397 (1%)
 Frame = +2

Query: 188  KDEFITVALADIRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTH 367
            K+E++ V L++IRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTH
Sbjct: 73   KEEYVVVKLSEIRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTH 132

Query: 368  CASRRSLRDDPNYDALISALYPDIDKYXXXXXXXXXXXRARNKQIQASIAQTLRRQSEAL 547
            CASRRSLRDDPNYDALISALYPDIDKY           +ARNKQIQA+IAQT  RQ+EAL
Sbjct: 133  CASRRSLRDDPNYDALISALYPDIDKYEEEELAFQEEEKARNKQIQATIAQTFHRQAEAL 192

Query: 548  VXXXXXXXXXXXXXXXXPQVXXXXXXXXXXXXXE---HQESDEEDNANDHDRAKDSSSAD 718
                                                  Q SD+ ++AN  D  KDSSSAD
Sbjct: 193  SRKKTTAKVTAAAFARRTPSRFRDAHSRGRRNYRMAGFQGSDDNEDANG-DGGKDSSSAD 251

Query: 719  EQVTEVKPKRNRRWGGFRXXXXXXXXXXXXXXXXXXXEVNRDSLGASAGLVGNSEMLAWG 898
            E   EVKPKR ++W G R                   EVN++S+G S+GLVG+SE LAWG
Sbjct: 252  EHSAEVKPKRCKKWAGARSAAVNADGGGDENDS----EVNKESVGGSSGLVGSSERLAWG 307

Query: 899  RGGTRSNTRHGSVNAGNGKQARNMRLSKLIDCIQNTVQNA-QLNIHLILVTLDKQRIPSL 1075
            + G RS+TR+GS N  N K AR+ R+SKL D ++N   N  +L+I+L+LV+ D+QR+PSL
Sbjct: 308  KNGMRSHTRYGSANGSNVKNARSSRISKLADYLRNLEGNDNELDINLMLVSFDEQRVPSL 367

Query: 1076 QCPHIRCSPTMSIRNLCQFVGLKTSVEADDVEILLVKGLHSEADTTLTSSVSISS-RTID 1252
            Q P++ C PT+SI  LCQ+V  +TS++A++VEI LV+ ++S+ D +++ S  IS    ID
Sbjct: 368  QRPYLCCRPTLSINKLCQYVAFQTSLQANEVEIYLVREMNSKVDFSISMSTPISKPGIID 427

Query: 1253 PCKDELHILEEHETLAELQAMYSVNGRELLFAYRQKL 1363
            PCKD+L +LEE ETL  L+    ++G  LL AYR+K+
Sbjct: 428  PCKDKLQVLEEQETLGRLKTNNFIHG-YLLLAYRKKV 463


>emb|CBI21880.3| unnamed protein product [Vitis vinifera]
          Length = 568

 Score =  380 bits (975), Expect = e-103
 Identities = 211/403 (52%), Positives = 261/403 (64%), Gaps = 11/403 (2%)
 Frame = +2

Query: 185  DKDEFITVALADIRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRT 364
            +K EF+ V L DIRK+VQCPICLGII+KTRTVMECLHRFCRECIDKSMRLGNNECPACRT
Sbjct: 163  EKPEFVFVGLPDIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRT 222

Query: 365  HCASRRSLRDDPNYDALISALYPDIDKYXXXXXXXXXXXRARNKQIQASIAQTLRRQSEA 544
            HCASRRSLRDDPNYDALI+ALYPDIDKY           + RNKQIQASIAQ L+RQSEA
Sbjct: 223  HCASRRSLRDDPNYDALIAALYPDIDKYEEEELAFHEEEQTRNKQIQASIAQILQRQSEA 282

Query: 545  LVXXXXXXXXXXXXXXXXPQ---VXXXXXXXXXXXXXEHQESDEEDNANDHDRAKDSSSA 715
            L                  Q                 + Q SD+ ++ ND+D  K SSSA
Sbjct: 283  LNRKRTLGKDIAGPFMTRSQRNYRNAYSRRRKNGRGSDLQGSDDNEDENDNDGGKGSSSA 342

Query: 716  DEQVTEVKPKRNRRWGGFR--XXXXXXXXXXXXXXXXXXXEVNRDSLGASAGLVGNSEML 889
            +E+ TEVK +R +R  G R                     EVNR+S G S GLV N EML
Sbjct: 343  EERSTEVKRRRYKRRAGIRLIQPSSSSVANSDGGCIENDLEVNRESRGFSPGLVWNPEML 402

Query: 890  AWGRGGTRSNTRHGSVNAGNGKQARNMRLSKLIDCIQNTVQN-AQLNIHLILVTLDKQRI 1066
            AWGRGG RS+TRHGS +  N K AR+ RLSKL+D +++  +N  +L++HL+L++LDK+  
Sbjct: 403  AWGRGGARSHTRHGSSSGCNHKSARSTRLSKLVDYLRSLGENDNELDVHLMLISLDKEST 462

Query: 1067 PSLQCPHIRCSPTMSIRNLCQFVGLKTSVEADDVEILLVKGLHSEADTTLTSSVSISSR- 1243
            PSLQ P++ C PT+S+R+LC++V L+T + A++VEIL+VKG H++       S S+    
Sbjct: 463  PSLQEPYLCCRPTLSVRHLCEYVALQTPLRAEEVEILVVKGCHNDYQPFPNPSTSVDDLN 522

Query: 1244 ----TIDPCKDELHILEEHETLAELQAMYSVNGRELLFAYRQK 1360
                 IDP KDEL ILE  ETL  LQA  +    +L+ AYRQK
Sbjct: 523  PDPIVIDPSKDELQILEGQETLVGLQANGTSCRNQLVLAYRQK 565


>ref|XP_002274885.2| PREDICTED: putative E3 ubiquitin-protein ligase RING1a-like [Vitis
            vinifera]
          Length = 412

 Score =  375 bits (964), Expect = e-101
 Identities = 209/399 (52%), Positives = 258/399 (64%), Gaps = 11/399 (2%)
 Frame = +2

Query: 197  FITVALADIRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCAS 376
            F+ V L DIRK+VQCPICLGII+KTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCAS
Sbjct: 11   FVFVGLPDIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCAS 70

Query: 377  RRSLRDDPNYDALISALYPDIDKYXXXXXXXXXXXRARNKQIQASIAQTLRRQSEALVXX 556
            RRSLRDDPNYDALI+ALYPDIDKY           + RNKQIQASIAQ L+RQSEAL   
Sbjct: 71   RRSLRDDPNYDALIAALYPDIDKYEEEELAFHEEEQTRNKQIQASIAQILQRQSEALNRK 130

Query: 557  XXXXXXXXXXXXXXPQ---VXXXXXXXXXXXXXEHQESDEEDNANDHDRAKDSSSADEQV 727
                           Q                 + Q SD+ ++ ND+D  K SSSA+E+ 
Sbjct: 131  RTLGKDIAGPFMTRSQRNYRNAYSRRRKNGRGSDLQGSDDNEDENDNDGGKGSSSAEERS 190

Query: 728  TEVKPKRNRRWGGFR--XXXXXXXXXXXXXXXXXXXEVNRDSLGASAGLVGNSEMLAWGR 901
            TEVK +R +R  G R                     EVNR+S G S GLV N EMLAWGR
Sbjct: 191  TEVKRRRYKRRAGIRLIQPSSSSVANSDGGCIENDLEVNRESRGFSPGLVWNPEMLAWGR 250

Query: 902  GGTRSNTRHGSVNAGNGKQARNMRLSKLIDCIQNTVQN-AQLNIHLILVTLDKQRIPSLQ 1078
            GG RS+TRHGS +  N K AR+ RLSKL+D +++  +N  +L++HL+L++LDK+  PSLQ
Sbjct: 251  GGARSHTRHGSSSGCNHKSARSTRLSKLVDYLRSLGENDNELDVHLMLISLDKESTPSLQ 310

Query: 1079 CPHIRCSPTMSIRNLCQFVGLKTSVEADDVEILLVKGLHSEADTTLTSSVSISSR----- 1243
             P++ C PT+S+R+LC++V L+T + A++VEIL+VKG H++       S S+        
Sbjct: 311  EPYLCCRPTLSVRHLCEYVALQTPLRAEEVEILVVKGCHNDYQPFPNPSTSVDDLNPDPI 370

Query: 1244 TIDPCKDELHILEEHETLAELQAMYSVNGRELLFAYRQK 1360
             IDP KDEL ILE  ETL  LQA  +    +L+ AYRQK
Sbjct: 371  VIDPSKDELQILEGQETLVGLQANGTSCRNQLVLAYRQK 409