BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00016237
         (1191 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265287.1| PREDICTED: ribosomal RNA large subunit methy...   562   e-158
ref|XP_002300266.1| predicted protein [Populus trichocarpa] gi|2...   558   e-156
ref|XP_004136937.1| PREDICTED: dual-specificity RNA methyltransf...   555   e-156
ref|NP_188597.1| radical SAM domain-containing protein [Arabidop...   551   e-155
dbj|BAB02548.1| unnamed protein product [Arabidopsis thaliana]        543   e-152

>ref|XP_002265287.1| PREDICTED: ribosomal RNA large subunit methyltransferase N [Vitis
            vinifera] gi|147817733|emb|CAN60150.1| hypothetical
            protein VITISV_044331 [Vitis vinifera]
            gi|296083052|emb|CBI22456.3| unnamed protein product
            [Vitis vinifera]
          Length = 372

 Score =  562 bits (1449), Expect = e-158
 Identities = 280/371 (75%), Positives = 309/371 (83%)
 Frame = +2

Query: 56   MGFRSVFDASVIRTEFEIAGINTQFIPLIWKYLLQNPNCEWEEIPSLPSAAYALLRSKFK 235
            M   S+FD   +R +F  AGI+  FIP IWKY++QNPNC+W++I SLPSAAY LLRS FK
Sbjct: 1    MAISSIFDGGELRRQFHSAGISPNFIPFIWKYVVQNPNCDWDDITSLPSAAYPLLRSHFK 60

Query: 236  PXXXXXXXXXXXXXQVTTKLLIKLQNGAFVEAVIMRYDSSLGILGGKPRPGGLRSTLCIS 415
            P              VTTKLLIKLQNG+FVEAVIMRYD+ LG  GGKPRPGG RSTLCIS
Sbjct: 61   PSTSSLHTVIDSSDNVTTKLLIKLQNGSFVEAVIMRYDTRLGKYGGKPRPGGPRSTLCIS 120

Query: 416  SQVGCKMGCTFCATGTMGFKNNLSSGEIVEQLVHASRISKIRNIVFMGMGEPLNNYNALI 595
            SQVGCKMGC FCATG+MGFK+NLSSGEIVEQLVHAS  S+IRN+VFMGMGEPLNNY+AL+
Sbjct: 121  SQVGCKMGCKFCATGSMGFKSNLSSGEIVEQLVHASHFSQIRNVVFMGMGEPLNNYSALV 180

Query: 596  EAIQVMTTSPFHLSPKKITVSTVGIIHAIKKFHGDMPNVNLAVSLHAPVQDIRCQIMPAA 775
            EAI +M  SPF LSPKKITVSTVGIIHAI K   D+PN+NLAVSLHAPVQDIRCQIMPAA
Sbjct: 181  EAIHIMQGSPFQLSPKKITVSTVGIIHAINKLQSDLPNLNLAVSLHAPVQDIRCQIMPAA 240

Query: 776  RAFPLEKLMAALQEYQNNSQQKIFIEYIMLDGINDEEQHAHQLGRLLETFEVVVNLIPFN 955
            RAFPLEKLM  L+ YQ NS QKIFIEYIMLD +NDEEQHAHQLG+LLETF+VVVNLIPFN
Sbjct: 241  RAFPLEKLMDTLRTYQTNSGQKIFIEYIMLDEVNDEEQHAHQLGKLLETFQVVVNLIPFN 300

Query: 956  PIGSSSHFGTSSEQKVVIFQKILRGTYEIRTTVRKQMGQDISGACGQLVINMPSKRSVTN 1135
            PIG+ S+F TSSEQKV  FQKILRGTY IRTTVRKQMGQDISGACGQLV+N P KRS  +
Sbjct: 301  PIGNLSYFKTSSEQKVARFQKILRGTYNIRTTVRKQMGQDISGACGQLVVNQPDKRSTGS 360

Query: 1136 AAPLADIEDLN 1168
            A  L DIEDL+
Sbjct: 361  AGLLTDIEDLH 371


>ref|XP_002300266.1| predicted protein [Populus trichocarpa] gi|222847524|gb|EEE85071.1|
            predicted protein [Populus trichocarpa]
          Length = 373

 Score =  558 bits (1438), Expect = e-156
 Identities = 278/370 (75%), Positives = 311/370 (84%)
 Frame = +2

Query: 56   MGFRSVFDASVIRTEFEIAGINTQFIPLIWKYLLQNPNCEWEEIPSLPSAAYALLRSKFK 235
            MG  S+FD   +R EFE AGINT FIP IWKY+++NPNCEW++IP LPSAAY+LLRSKFK
Sbjct: 1    MGVGSIFDGPDVRAEFEKAGINTHFIPFIWKYVIKNPNCEWDDIPDLPSAAYSLLRSKFK 60

Query: 236  PXXXXXXXXXXXXXQVTTKLLIKLQNGAFVEAVIMRYDSSLGILGGKPRPGGLRSTLCIS 415
                           VTTKLL+KLQNGAFVEAVIMRYD+ LG   GKPRPGG RSTLCIS
Sbjct: 61   TSTSSVDSVINSNDGVTTKLLVKLQNGAFVEAVIMRYDTRLGKYCGKPRPGGPRSTLCIS 120

Query: 416  SQVGCKMGCTFCATGTMGFKNNLSSGEIVEQLVHASRISKIRNIVFMGMGEPLNNYNALI 595
            SQVGCKMGC FCATG+MGFKNNLSSGEIVEQLVHAS +S+IRN+VFMGMGEPLNNY+AL+
Sbjct: 121  SQVGCKMGCKFCATGSMGFKNNLSSGEIVEQLVHASCLSQIRNVVFMGMGEPLNNYSALV 180

Query: 596  EAIQVMTTSPFHLSPKKITVSTVGIIHAIKKFHGDMPNVNLAVSLHAPVQDIRCQIMPAA 775
            EA++ M+  PF LSPK+ITVSTVGIIHAI K H D+P +NLAVSLHAPVQD+RCQIMPAA
Sbjct: 181  EAVRAMSGVPFQLSPKRITVSTVGIIHAINKLHKDLPGLNLAVSLHAPVQDVRCQIMPAA 240

Query: 776  RAFPLEKLMAALQEYQNNSQQKIFIEYIMLDGINDEEQHAHQLGRLLETFEVVVNLIPFN 955
            RAFPLEKLM ALQ YQ NS QKIFIEYIMLDG+NDEEQHAHQLG+LLETF+VVVNLIPFN
Sbjct: 241  RAFPLEKLMDALQVYQKNSMQKIFIEYIMLDGVNDEEQHAHQLGKLLETFDVVVNLIPFN 300

Query: 956  PIGSSSHFGTSSEQKVVIFQKILRGTYEIRTTVRKQMGQDISGACGQLVINMPSKRSVTN 1135
            PIGS S F TSSE+KV+ FQKILRG   IRTTVRKQMGQDISGACGQLV+N+P ++   N
Sbjct: 301  PIGSLSQFRTSSEEKVLRFQKILRGVNNIRTTVRKQMGQDISGACGQLVVNLPDEKKPPN 360

Query: 1136 AAPLADIEDL 1165
               + DIEDL
Sbjct: 361  LG-VTDIEDL 369


>ref|XP_004136937.1| PREDICTED: dual-specificity RNA methyltransferase RlmN 1-like
            [Cucumis sativus] gi|449495695|ref|XP_004159917.1|
            PREDICTED: dual-specificity RNA methyltransferase RlmN
            1-like [Cucumis sativus]
          Length = 384

 Score =  555 bits (1430), Expect = e-156
 Identities = 277/380 (72%), Positives = 318/380 (83%), Gaps = 11/380 (2%)
 Frame = +2

Query: 62   FRSVFDASVIRTEFEIAGINTQFIPLIWKYLLQNPN----------CEWEEI-PSLPSAA 208
            FRS FDA  +RTEFE AGI T FIP IWK+LL +PN           +WE++ PSLPSAA
Sbjct: 4    FRSAFDAGELRTEFEKAGIKTTFIPFIWKHLLHDPNNIFNHNLASEFQWEQVVPSLPSAA 63

Query: 209  YALLRSKFKPXXXXXXXXXXXXXQVTTKLLIKLQNGAFVEAVIMRYDSSLGILGGKPRPG 388
            Y+LLR KFKP             QVTTKLLIKLQ+GAFVEAVIMRYD+ LG  GGKPRPG
Sbjct: 64   YSLLRLKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKPRPG 123

Query: 389  GLRSTLCISSQVGCKMGCTFCATGTMGFKNNLSSGEIVEQLVHASRISKIRNIVFMGMGE 568
            GLRSTLCISSQVGCKMGC FCATGTMGFK+NL+SGEIVEQLVHASR S+IRNIVFMGMGE
Sbjct: 124  GLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEIVEQLVHASRFSQIRNIVFMGMGE 183

Query: 569  PLNNYNALIEAIQVMTTSPFHLSPKKITVSTVGIIHAIKKFHGDMPNVNLAVSLHAPVQD 748
            PLNNY++L+EA++ M   PF LSPK+IT+STVGIIHAI K H D+P +NLAVSLHAPVQD
Sbjct: 184  PLNNYSSLVEAVRAMMAPPFLLSPKRITISTVGIIHAINKLHSDLPGLNLAVSLHAPVQD 243

Query: 749  IRCQIMPAARAFPLEKLMAALQEYQNNSQQKIFIEYIMLDGINDEEQHAHQLGRLLETFE 928
            IRCQIMPAARAFPL+KLM ALQEYQ  SQQKI IEYIMLDG+NDEEQHAHQLG+LLETF+
Sbjct: 244  IRCQIMPAARAFPLQKLMDALQEYQKKSQQKILIEYIMLDGVNDEEQHAHQLGKLLETFQ 303

Query: 929  VVVNLIPFNPIGSSSHFGTSSEQKVVIFQKILRGTYEIRTTVRKQMGQDISGACGQLVIN 1108
            V+VNLIPFNPIGSSS F TSS++KV +FQ++LRGTY IRTT+RKQMGQDISGACGQLV++
Sbjct: 304  VIVNLIPFNPIGSSSQFKTSSDEKVSVFQQVLRGTYNIRTTIRKQMGQDISGACGQLVVS 363

Query: 1109 MPSKRSVTNAAPLADIEDLN 1168
            +P++ S   ++ + DIEDL+
Sbjct: 364  LPNRSSSKQSSVVPDIEDLH 383


>ref|NP_188597.1| radical SAM domain-containing protein [Arabidopsis thaliana]
            gi|28393068|gb|AAO41968.1| unknown protein [Arabidopsis
            thaliana] gi|28827394|gb|AAO50541.1| unknown protein
            [Arabidopsis thaliana] gi|332642748|gb|AEE76269.1|
            radical SAM domain-containing protein [Arabidopsis
            thaliana]
          Length = 372

 Score =  551 bits (1421), Expect = e-155
 Identities = 271/370 (73%), Positives = 311/370 (84%)
 Frame = +2

Query: 56   MGFRSVFDASVIRTEFEIAGINTQFIPLIWKYLLQNPNCEWEEIPSLPSAAYALLRSKFK 235
            M  +SVFDAS I++EFE AGIN +F   IWKY++QNP+C W+EIPSLPSAAY+LL SKFK
Sbjct: 1    MKLKSVFDASEIKSEFESAGINPKFAIQIWKYVIQNPDCVWDEIPSLPSAAYSLLHSKFK 60

Query: 236  PXXXXXXXXXXXXXQVTTKLLIKLQNGAFVEAVIMRYDSSLGILGGKPRPGGLRSTLCIS 415
                            T+KLLIKLQNGAFVEAV+MRYD+ LG+LGGKPRPGG+RSTLCIS
Sbjct: 61   TLTSSLHSLFHSSDGTTSKLLIKLQNGAFVEAVVMRYDTRLGMLGGKPRPGGIRSTLCIS 120

Query: 416  SQVGCKMGCTFCATGTMGFKNNLSSGEIVEQLVHASRISKIRNIVFMGMGEPLNNYNALI 595
            SQVGCKMGCTFCATGTMGFK+NL+SGEIVEQLVHASRI+ IRNIVFMGMGEPLNNYNA++
Sbjct: 121  SQVGCKMGCTFCATGTMGFKSNLTSGEIVEQLVHASRIADIRNIVFMGMGEPLNNYNAVV 180

Query: 596  EAIQVMTTSPFHLSPKKITVSTVGIIHAIKKFHGDMPNVNLAVSLHAPVQDIRCQIMPAA 775
            EA++VM   PF LSPK+IT+STVGI+HAI K H D+P V+LAVSLHAPVQ+IRCQIMPAA
Sbjct: 181  EAVRVMLNQPFQLSPKRITISTVGIVHAINKLHNDLPGVSLAVSLHAPVQEIRCQIMPAA 240

Query: 776  RAFPLEKLMAALQEYQNNSQQKIFIEYIMLDGINDEEQHAHQLGRLLETFEVVVNLIPFN 955
            RAFPL+KLM ALQ +Q NSQQKIFIEYIMLDG+ND+EQHAH LG LL+TF+VV+NLIPFN
Sbjct: 241  RAFPLQKLMDALQTFQKNSQQKIFIEYIMLDGVNDQEQHAHLLGELLKTFQVVINLIPFN 300

Query: 956  PIGSSSHFGTSSEQKVVIFQKILRGTYEIRTTVRKQMGQDISGACGQLVINMPSKRSVTN 1135
            PIGS+S F TSS Q V  FQKILR TY+IRTT+RK+MGQDISGACGQLV+N P  +    
Sbjct: 301  PIGSTSQFETSSIQGVSRFQKILRETYKIRTTIRKEMGQDISGACGQLVVNQPDIKKTPG 360

Query: 1136 AAPLADIEDL 1165
               L DIEDL
Sbjct: 361  TVELRDIEDL 370


>dbj|BAB02548.1| unnamed protein product [Arabidopsis thaliana]
          Length = 382

 Score =  543 bits (1400), Expect = e-152
 Identities = 271/380 (71%), Positives = 311/380 (81%), Gaps = 10/380 (2%)
 Frame = +2

Query: 56   MGFRSVFDASVIRTEFEIAGINTQFIPLIWKYLLQNPNCEWEEIPSLPSAAYALLRSKFK 235
            M  +SVFDAS I++EFE AGIN +F   IWKY++QNP+C W+EIPSLPSAAY+LL SKFK
Sbjct: 1    MKLKSVFDASEIKSEFESAGINPKFAIQIWKYVIQNPDCVWDEIPSLPSAAYSLLHSKFK 60

Query: 236  PXXXXXXXXXXXXXQVTTKLLIKLQNGAFVEAVIMRYDSSLGILGGKPRPGGLRSTLCIS 415
                            T+KLLIKLQNGAFVEAV+MRYD+ LG+LGGKPRPGG+RSTLCIS
Sbjct: 61   TLTSSLHSLFHSSDGTTSKLLIKLQNGAFVEAVVMRYDTRLGMLGGKPRPGGIRSTLCIS 120

Query: 416  SQVGCKMGCTFCATGTMGFKNNLSSGEIVEQLVHASRISKIRNIVFM----------GMG 565
            SQVGCKMGCTFCATGTMGFK+NL+SGEIVEQLVHASRI+ IRNIVFM          GMG
Sbjct: 121  SQVGCKMGCTFCATGTMGFKSNLTSGEIVEQLVHASRIADIRNIVFMLLPWFDDCDQGMG 180

Query: 566  EPLNNYNALIEAIQVMTTSPFHLSPKKITVSTVGIIHAIKKFHGDMPNVNLAVSLHAPVQ 745
            EPLNNYNA++EA++VM   PF LSPK+IT+STVGI+HAI K H D+P V+LAVSLHAPVQ
Sbjct: 181  EPLNNYNAVVEAVRVMLNQPFQLSPKRITISTVGIVHAINKLHNDLPGVSLAVSLHAPVQ 240

Query: 746  DIRCQIMPAARAFPLEKLMAALQEYQNNSQQKIFIEYIMLDGINDEEQHAHQLGRLLETF 925
            +IRCQIMPAARAFPL+KLM ALQ +Q NSQQKIFIEYIMLDG+ND+EQHAH LG LL+TF
Sbjct: 241  EIRCQIMPAARAFPLQKLMDALQTFQKNSQQKIFIEYIMLDGVNDQEQHAHLLGELLKTF 300

Query: 926  EVVVNLIPFNPIGSSSHFGTSSEQKVVIFQKILRGTYEIRTTVRKQMGQDISGACGQLVI 1105
            +VV+NLIPFNPIGS+S F TSS Q V  FQKILR TY+IRTT+RK+MGQDISGACGQLV+
Sbjct: 301  QVVINLIPFNPIGSTSQFETSSIQGVSRFQKILRETYKIRTTIRKEMGQDISGACGQLVV 360

Query: 1106 NMPSKRSVTNAAPLADIEDL 1165
            N P  +       L DIEDL
Sbjct: 361  NQPDIKKTPGTVELRDIEDL 380