BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00016191
         (1161 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002263131.2| PREDICTED: probable 2-oxoglutarate/Fe(II)-de...   491   e-136
gb|AAM12973.1| strong similarity to naringenin 3-dioxygenase [Ar...   456   e-126
ref|XP_002868142.1| hypothetical protein ARALYDRAFT_355126 [Arab...   455   e-126
ref|XP_003537378.1| PREDICTED: probable 2-oxoglutarate/Fe(II)-de...   440   e-121
ref|XP_003553156.1| PREDICTED: naringenin,2-oxoglutarate 3-dioxy...   439   e-121

>ref|XP_002263131.2| PREDICTED: probable 2-oxoglutarate/Fe(II)-dependent dioxygenase-like
            [Vitis vinifera]
          Length = 355

 Score =  491 bits (1264), Expect = e-136
 Identities = 248/352 (70%), Positives = 280/352 (79%), Gaps = 16/352 (4%)
 Frame = -2

Query: 1121 MEVAAGEPVRVQSLS-----QVPPQYIQPPETRPNN--PHTSPTLSSAIPTIDLSIHR-- 969
            MEV  GEP RVQ+L+     QVPP+YIQ PE RP    P T    SS +P IDL      
Sbjct: 1    MEVVVGEPTRVQTLAEKNPTQVPPEYIQAPENRPQIKIPTTEDNTSSTVPVIDLFGFDPD 60

Query: 968  RATVIRREIAGACRDWGAFHVINHGVPIELLNDMRNNGRAFFEDHDLQGKLKYSCTPDSP 789
                +RRE+  AC++WGAF V NHGVP  LL+ MR+ G +FF+D  +  KL Y+C P S 
Sbjct: 61   HWGDVRRELGQACKEWGAFQVTNHGVPFHLLHRMRSVGLSFFKDSPMSHKLSYACPPGSA 120

Query: 788  ASEGYGSRMLVQ-SNDTVLDWRDYFDHHTLPVSRRNPSNWPLSPPNYREVVVEYSDRMEA 612
            ASEGYGSRMLV  SNDTVLDWRD+FDHHTLP+SRRNPS WP  PPNYREVVVEYSD M+A
Sbjct: 121  ASEGYGSRMLVSTSNDTVLDWRDFFDHHTLPLSRRNPSRWPDFPPNYREVVVEYSDCMKA 180

Query: 611  LAQKLMRLISESLGLPTSCIEEAVGEFYQNITVSYYPPCPQPELTLGLQSHSDMGAITLL 432
            LAQKL+  +SESLGLP+SCIE+AVG+FYQNITVSYYPPCPQPELTLGLQSHSDMGAITLL
Sbjct: 181  LAQKLLAFMSESLGLPSSCIEDAVGDFYQNITVSYYPPCPQPELTLGLQSHSDMGAITLL 240

Query: 431  IQDDVGGLQVLKDGEWVTVHPVSDAIVVILADQTEVITNGQYRSAQHRAITNSSRARLSV 252
            IQDDVGGLQVLKDGEWV V P+SDAIVVILADQTE+ITNG YRSAQHRAI N+++ARLSV
Sbjct: 241  IQDDVGGLQVLKDGEWVMVQPLSDAIVVILADQTEIITNGNYRSAQHRAIANANKARLSV 300

Query: 251  ATFHDPGKTTKVSPV------IHPPRYKQVIYGDYVSSWYTKGPEGKRNIEA 114
            ATFHDP KT K+SP         PPRY Q++YG+YVSSWYTKGPEGKRNI+A
Sbjct: 301  ATFHDPAKTMKISPASALVTESFPPRYCQIVYGEYVSSWYTKGPEGKRNIDA 352


>gb|AAM12973.1| strong similarity to naringenin 3-dioxygenase [Arabidopsis thaliana]
            gi|21387115|gb|AAM47961.1| strong similarity to
            naringenin 3-dioxygenase [Arabidopsis thaliana]
          Length = 338

 Score =  456 bits (1174), Expect = e-126
 Identities = 225/340 (66%), Positives = 273/340 (80%), Gaps = 4/340 (1%)
 Frame = -2

Query: 1121 MEVAAGEPVRVQ-SLSQVPPQYIQPPETRPNNPHTSPTLSSAIPTIDLSIHRRATVIRRE 945
            ME A+   + V+ +L +VP QYIQPPE RPN  ++    S  IPT+DLS    A   R  
Sbjct: 1    METASRVELIVKGNLPEVPSQYIQPPEARPNLHYSGDAAS--IPTVDLSSSDSA---REA 55

Query: 944  IAGACRDWGAFHVINHGVPIELLNDMRNNGRAFFEDHDLQGKLKYSCTPDSPASEGYGSR 765
            I  ACRDWGAFHVINHGVPI LL+ MR+ G +FF+D  ++ KL+Y+C   S ASEGYGSR
Sbjct: 56   IGDACRDWGAFHVINHGVPIHLLDRMRSLGLSFFQDSPMEEKLRYACDSTSAASEGYGSR 115

Query: 764  MLVQS-NDTVLDWRDYFDHHTLPVSRRNPSNWPLSPPNYREVVVEYSDRMEALAQKLMRL 588
            ML+ + +D VLDWRDYFDHHT P SRRNPS+WP+ P +YR+VV EY D M+ LAQ L+ L
Sbjct: 116  MLLGAKDDVVLDWRDYFDHHTFPPSRRNPSHWPIHPSDYRQVVGEYGDEMKKLAQMLLGL 175

Query: 587  ISESLGLPTSCIEEAVGEFYQNITVSYYPPCPQPELTLGLQSHSDMGAITLLIQDDVGGL 408
            ISESLGLP S IEEAVGE YQNITVSYYPPCPQPELTLGLQSHSD GAITLLIQDDV GL
Sbjct: 176  ISESLGLPCSSIEEAVGEIYQNITVSYYPPCPQPELTLGLQSHSDFGAITLLIQDDVEGL 235

Query: 407  QVLKDGEWVTVHPVSDAIVVILADQTEVITNGQYRSAQHRAITNSSRARLSVATFHDPGK 228
            Q+ KD +W+TV P+SDAI++++ADQTE+ITNG+Y+SAQHRA+TN++RARLSVATFHDP K
Sbjct: 236  QLYKDAQWLTVPPISDAILILIADQTEIITNGRYKSAQHRAVTNANRARLSVATFHDPSK 295

Query: 227  TTKVSPV--IHPPRYKQVIYGDYVSSWYTKGPEGKRNIEA 114
            T +++PV  + PP YK+V+YG YVSSWY+KGPEGKRN++A
Sbjct: 296  TARIAPVSQLSPPSYKEVVYGQYVSSWYSKGPEGKRNLDA 335


>ref|XP_002868142.1| hypothetical protein ARALYDRAFT_355126 [Arabidopsis lyrata subsp.
            lyrata] gi|297313978|gb|EFH44401.1| hypothetical protein
            ARALYDRAFT_355126 [Arabidopsis lyrata subsp. lyrata]
          Length = 338

 Score =  455 bits (1171), Expect = e-126
 Identities = 225/340 (66%), Positives = 273/340 (80%), Gaps = 4/340 (1%)
 Frame = -2

Query: 1121 MEVAAGEPVRVQ-SLSQVPPQYIQPPETRPNNPHTSPTLSSAIPTIDLSIHRRATVIRRE 945
            ME A+   + V+ +L +VP QYIQPPE RP+  ++    S  IPT+DLS    A   R  
Sbjct: 1    METASRVELIVKGNLPEVPSQYIQPPEARPHLHYSGDAAS--IPTVDLSSSNSA---REA 55

Query: 944  IAGACRDWGAFHVINHGVPIELLNDMRNNGRAFFEDHDLQGKLKYSCTPDSPASEGYGSR 765
            I  ACR+WGAFHVINHGVPI LL+ MR+ G +FF+D  ++ KL+Y+C   S ASEGYGSR
Sbjct: 56   IGDACRNWGAFHVINHGVPIHLLDRMRSLGLSFFQDSPMEEKLRYACDSTSAASEGYGSR 115

Query: 764  MLVQS-NDTVLDWRDYFDHHTLPVSRRNPSNWPLSPPNYREVVVEYSDRMEALAQKLMRL 588
            ML+ + +D VLDWRDYFDHHT P+SRRNPS WP+ PP+YR+VV EY D M+ LAQ L+ L
Sbjct: 116  MLLGAKDDVVLDWRDYFDHHTFPLSRRNPSRWPIHPPDYRQVVGEYGDEMKKLAQMLLEL 175

Query: 587  ISESLGLPTSCIEEAVGEFYQNITVSYYPPCPQPELTLGLQSHSDMGAITLLIQDDVGGL 408
            ISESLGLP S IEEAVGE YQNITVSYYPPCPQPELTLGLQSHSDMGAITLLIQDDVGGL
Sbjct: 176  ISESLGLPGSSIEEAVGEIYQNITVSYYPPCPQPELTLGLQSHSDMGAITLLIQDDVGGL 235

Query: 407  QVLKDGEWVTVHPVSDAIVVILADQTEVITNGQYRSAQHRAITNSSRARLSVATFHDPGK 228
            Q+ KD +W+TV P S AI+V++ADQTE+ITNG Y+SAQHRA+TN+++ARLSVATFHDP K
Sbjct: 236  QLYKDAQWLTVPPNSHAILVLIADQTEIITNGLYKSAQHRAVTNANQARLSVATFHDPSK 295

Query: 227  TTKVSPV--IHPPRYKQVIYGDYVSSWYTKGPEGKRNIEA 114
            T +++PV  + PP YK+V+YG YVSSWY+KGPEGKRN++A
Sbjct: 296  TARIAPVSQLSPPSYKEVVYGQYVSSWYSKGPEGKRNLDA 335


>ref|XP_003537378.1| PREDICTED: probable 2-oxoglutarate/Fe(II)-dependent dioxygenase-like
            [Glycine max]
          Length = 441

 Score =  440 bits (1132), Expect = e-121
 Identities = 230/364 (63%), Positives = 269/364 (73%), Gaps = 28/364 (7%)
 Frame = -2

Query: 1121 MEVAAGEPV---RVQSLSQ-----VPPQYIQPPETRPNNPHTSPTLSSAIPTIDLSIH-- 972
            M+V + E +   RVQSL+Q     VPPQYIQPP+TRP       + S +IP IDL     
Sbjct: 75   MQVVSDEAIMTTRVQSLAQSGLSRVPPQYIQPPQTRPIRHAPPESDSESIPVIDLCSFDP 134

Query: 971  -RRATVIRREIAGACRDWGAFHVINHGVPIELLNDMRNNGRAFFEDHDLQGKLKYSCTPD 795
             +RA+  R  IA ACR WGAFHV NHGVP  LL  +R  G +FF D  +  KL+YSC+  
Sbjct: 135  TQRAST-RDSIARACRHWGAFHVTNHGVPPSLLASLRRAGLSFFSDTPMPDKLRYSCSA- 192

Query: 794  SPASEGYGSRMLVQSNDT----------VLDWRDYFDHHTLPVSRRNPSNWPLSPPNYRE 645
            + ASEGYGS+ML  +  T          VLDWRDYFDHHTLP+SRRNP+ WP SP +YRE
Sbjct: 193  AAASEGYGSKMLATTTTTTTSDQNGAVQVLDWRDYFDHHTLPLSRRNPTRWPESPSDYRE 252

Query: 644  VVVEYSDRMEALAQKLMRLISESLGLPTSCIEEAVGEFYQNITVSYYPPCPQPELTLGLQ 465
            +V  YSD M  LAQKL+ LISESLGL  SCIE+AVGEFYQNIT+SYYPPCP+P+LTLGLQ
Sbjct: 253  LVARYSDEMNVLAQKLLALISESLGLRASCIEDAVGEFYQNITISYYPPCPEPDLTLGLQ 312

Query: 464  SHSDMGAITLLIQDDVGGLQVLKDGE-WVTVHPVSDAIVVILADQTEVITNGQYRSAQHR 288
            SHSDMGAITLLIQDDVGGLQVLK  + WVTV P+SDA++V+LADQTE+ITNG+YRS +HR
Sbjct: 313  SHSDMGAITLLIQDDVGGLQVLKGSDKWVTVQPLSDAVLVLLADQTEIITNGKYRSCEHR 372

Query: 287  AITNSSRARLSVATFHDPGKTTKVSPVIH------PPRYKQVIYGDYVSSWYTKGPEGKR 126
            AITN  RARLSVATFHDP KT K+SP         P +Y+ V+YGDYVSSWYTKGP GKR
Sbjct: 373  AITNPDRARLSVATFHDPAKTAKISPASELINDSSPAKYRDVVYGDYVSSWYTKGPGGKR 432

Query: 125  NIEA 114
            NI+A
Sbjct: 433  NIDA 436


>ref|XP_003553156.1| PREDICTED: naringenin,2-oxoglutarate 3-dioxygenase-like [Glycine max]
          Length = 351

 Score =  439 bits (1128), Expect = e-121
 Identities = 232/348 (66%), Positives = 268/348 (77%), Gaps = 21/348 (6%)
 Frame = -2

Query: 1094 RVQSLSQ-----VPPQYIQPPETRPNNPHTSPTLSSAIPTIDLSIH---RRATVIRREIA 939
            RVQSL+Q     VPPQYIQPP+TRP   HT+P   S IP IDLS     +RA+  R  IA
Sbjct: 4    RVQSLAQSGLSRVPPQYIQPPQTRPVR-HTAPEPDS-IPVIDLSSFDPTQRAST-RDSIA 60

Query: 938  GACRDWGAFHVINHGVPIELLNDMRNNGRAFFEDHDLQGKLKYSCTPDSPASEGYGSRML 759
             ACR+WGAFHV NHGVP  LL  +R  G +FF D  +  KL+YSC+  + ASEGYGS+ML
Sbjct: 61   RACREWGAFHVTNHGVPPSLLASLRRAGLSFFSDTPIPDKLRYSCS--AAASEGYGSKML 118

Query: 758  VQS----NDTV--LDWRDYFDHHTLPVSRRNPSNWPLSPPNYREVVVEYSDRMEALAQKL 597
              +    ND V  LDWRDYFDHHTLP+SRRNP+ WP  P +YRE+V  YSD M+ LAQKL
Sbjct: 119  ATTTSDQNDAVQVLDWRDYFDHHTLPLSRRNPNRWPEFPADYRELVATYSDEMKILAQKL 178

Query: 596  MRLISESLGLPTSCIEEAVGEFYQNITVSYYPPCPQPELTLGLQSHSDMGAITLLIQDDV 417
            + LISESLGL  SCIE+AVGEFYQNIT+SYYPPCP+P+LTLGLQSHSDMGAITLLIQDDV
Sbjct: 179  LALISESLGLRASCIEDAVGEFYQNITISYYPPCPEPDLTLGLQSHSDMGAITLLIQDDV 238

Query: 416  GGLQVLKDG-EWVTVHPVSDAIVVILADQTEVITNGQYRSAQHRAITNSSRARLSVATFH 240
            GGLQVLK G +WVTV P+SDAI+V+LADQTE+ITNG+YRS +HRAITN  RARLSVATFH
Sbjct: 239  GGLQVLKGGNKWVTVQPLSDAILVLLADQTEIITNGKYRSCEHRAITNPDRARLSVATFH 298

Query: 239  DPGKTTKVSPVIH------PPRYKQVIYGDYVSSWYTKGPEGKRNIEA 114
            DP KT K+SP           +Y+ V+YGDYVSSWYTKGP GKRNI+A
Sbjct: 299  DPAKTVKISPASELINDSSLAKYRDVVYGDYVSSWYTKGPGGKRNIDA 346