BLASTX nr result
ID: Bupleurum21_contig00016013
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00016013
(1956 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002270360.1| PREDICTED: 4-coumarate--CoA ligase-like 6 [V... 707 0.0
ref|XP_002304364.1| 4-coumarate-coa ligase [Populus trichocarpa]... 700 0.0
ref|XP_003612659.1| 4-coumarate-CoA ligase [Medicago truncatula]... 695 0.0
ref|XP_003612661.1| 4-coumarate CoA ligase [Medicago truncatula]... 692 0.0
ref|XP_002304363.1| acyl:coa ligase [Populus trichocarpa] gi|222... 691 0.0
>ref|XP_002270360.1| PREDICTED: 4-coumarate--CoA ligase-like 6 [Vitis vinifera]
gi|296083381|emb|CBI23270.3| unnamed protein product
[Vitis vinifera]
Length = 571
Score = 707 bits (1825), Expect = 0.0
Identities = 356/561 (63%), Positives = 432/561 (77%), Gaps = 1/561 (0%)
Frame = -3
Query: 1891 SRTQETNVNKISNIQNPHDQPSWFSPQTGVYTSIYTPVNLPSDPFLDVVSFIFSRKHNGV 1712
S+ Q TN + S+ +P+ P WFSP+TG+Y+S Y + +PSDP LD+VSFIFS+ HNGV
Sbjct: 11 SQNQATNTQRASDNTHPNGHPHWFSPETGIYSSTYPSLPIPSDPHLDIVSFIFSKNHNGV 70
Query: 1711 SALIDSSSGFSISYQELYSLVRSVASGLQKIGVKQGDVVFILLPNSVYFPVILLGVVYMG 1532
SAL D SSGFSISY +L+ LV+S+ASGL ++G QGDV+ +LLPNS+YFPVI L V+ +G
Sbjct: 71 SALTDYSSGFSISYSQLHPLVKSMASGLHQMGFSQGDVILLLLPNSIYFPVIFLAVLSLG 130
Query: 1531 AIVSTMNPLSSLSELKKQTLDSHAKFVFTLLKNVDKLSALGVSAIAVPERFDFDSIPSEF 1352
I +TMNPLSSL+E++KQT+ FT+ + +KL ALGV I VPE F+S EF
Sbjct: 131 GIATTMNPLSSLTEIRKQTVGCSLSLAFTVPETSEKLGALGVPVIGVPENGSFNSKSKEF 190
Query: 1351 SCFYDLISSDSSLMTKPIIKQQDTAAIMHSSGTTGTSKGVVLTHMNFIAMVELFVRFEAS 1172
S F+ LIS D +P+I Q D AAIM+SSGTTG++K VVLTH NFIAMVE FVRFEAS
Sbjct: 191 STFHWLISGDPDCCPRPVINQHDVAAIMYSSGTTGSTKAVVLTHGNFIAMVETFVRFEAS 250
Query: 1171 QYQLPSWENVYLAVLPMFHIYGLSLFVMGLLSLGSSIVVMSRYNVDEMPTVIDRYGVTHF 992
Y+ ENVYLAVLPMFHIYGLSLFV GL+SLGS+IVVM +++ +EM IDRY VTHF
Sbjct: 251 LYENSGSENVYLAVLPMFHIYGLSLFVTGLISLGSAIVVMRKFDANEMVKAIDRYRVTHF 310
Query: 991 PVVPPLLAALTKAAHTVARSKLKSLKQVSCGAASLNTKSIQDFVRALPHVDFIQGYGMTE 812
PVVPP+L AL K+A SLKQV CGAA L KSIQ+FV+ L HVD IQGYGMTE
Sbjct: 311 PVVPPVLMALIKSARAAGAGCFGSLKQVCCGAAPLTQKSIQEFVQTLSHVDLIQGYGMTE 370
Query: 811 STAVATRGFNTESLHNYSSVGLLSPNMEAKIVDSLTGVFKGPGGMGELWLRGPSIMKGYL 632
STAV TRGFNT+ L NYSS+GLL+PNM AK+VD +G PG GELWL+GP IMKGYL
Sbjct: 371 STAVGTRGFNTKKLRNYSSIGLLAPNMRAKVVDLSSGSLLPPGNCGELWLQGPGIMKGYL 430
Query: 631 NNVEETLSTIDKEGWLHTGDICYFDNNGYLYLIDRLKEIIKYNGFQIAPAELEAILLSHP 452
N+ E T+STIDKEGWLHTGDI FD +GYLY+ R+KEIIKY GFQIAP +LE+IL+SHP
Sbjct: 431 NDEEATISTIDKEGWLHTGDIVSFDQDGYLYMFSRIKEIIKYKGFQIAPVDLESILISHP 490
Query: 451 EVADVAVTAGRDEAAGEVPVAFVLRRDGSRISEAALIDYVAKQVSPYKKVRKVVFTSSIP 272
E++D AV DE AGEVPVAFV++R GS +S+AA+I+YV KQV+PYKKVRKV+FT IP
Sbjct: 491 EISDAAVAGVGDEEAGEVPVAFVVKRPGSALSQAAIINYVEKQVAPYKKVRKVIFTHPIP 550
Query: 271 RSAAGKILRRELKS-FLPSKI 212
+SAAGKILRRELK F+ S+I
Sbjct: 551 KSAAGKILRRELKHLFMISRI 571
>ref|XP_002304364.1| 4-coumarate-coa ligase [Populus trichocarpa]
gi|222841796|gb|EEE79343.1| 4-coumarate-coa ligase
[Populus trichocarpa]
Length = 544
Score = 700 bits (1806), Expect = 0.0
Identities = 350/541 (64%), Positives = 428/541 (79%), Gaps = 1/541 (0%)
Frame = -3
Query: 1831 PSWFSPQTGVYTSIYTPVNLPSDPFLDVVSFIFSR-KHNGVSALIDSSSGFSISYQELYS 1655
P W+SP+TG+Y+S + P+ LP+DPFLDVVSFIFS HNG++ALIDSSSGFSISY ++
Sbjct: 14 PEWYSPETGIYSSKHPPIPLPADPFLDVVSFIFSHHNHNGLTALIDSSSGFSISYSKILP 73
Query: 1654 LVRSVASGLQKIGVKQGDVVFILLPNSVYFPVILLGVVYMGAIVSTMNPLSSLSELKKQT 1475
LV+SVASGL +G+KQGDVV +LLPNS++FP++ LGV+Y+G IVSTMNPLSS E+K++
Sbjct: 74 LVKSVASGLSNMGIKQGDVVLLLLPNSIHFPIVFLGVLYLGGIVSTMNPLSSELEVKQRI 133
Query: 1474 LDSHAKFVFTLLKNVDKLSALGVSAIAVPERFDFDSIPSEFSCFYDLISSDSSLMTKPII 1295
+D +A F L+ V K LG+ I VPE +FD + I S + KP+I
Sbjct: 134 VDCNACIAFVELEKVCKFQPLGIPVIGVPENVNFD----------EKIYSKGDVGVKPVI 183
Query: 1294 KQQDTAAIMHSSGTTGTSKGVVLTHMNFIAMVELFVRFEASQYQLPSWENVYLAVLPMFH 1115
+QQDTAAIM+SSGTT SKGVVLTH NFI+MVELFV+FEASQY+ S +NVYLAVLPMFH
Sbjct: 184 RQQDTAAIMYSSGTTAASKGVVLTHRNFISMVELFVKFEASQYEYSSTDNVYLAVLPMFH 243
Query: 1114 IYGLSLFVMGLLSLGSSIVVMSRYNVDEMPTVIDRYGVTHFPVVPPLLAALTKAAHTVAR 935
IYGLSLFV+GLLSLGSSIVVM +++V EM VIDRYGVTHFPVVPP+L ALT+ A V
Sbjct: 244 IYGLSLFVVGLLSLGSSIVVMRKFDVSEMVKVIDRYGVTHFPVVPPILTALTRTAKGVCG 303
Query: 934 SKLKSLKQVSCGAASLNTKSIQDFVRALPHVDFIQGYGMTESTAVATRGFNTESLHNYSS 755
+ LK LK VSCGAA L K+IQDFV LPHVDFIQGYG+TESTAV TRG NTE YSS
Sbjct: 304 NSLKCLKLVSCGAAPLFGKTIQDFVEVLPHVDFIQGYGLTESTAVGTRGLNTEKFQKYSS 363
Query: 754 VGLLSPNMEAKIVDSLTGVFKGPGGMGELWLRGPSIMKGYLNNVEETLSTIDKEGWLHTG 575
+GLL+PN+EAK+VD +TG PGG GELW+RGP +MK YLN E T TIDK+GWLHTG
Sbjct: 364 IGLLAPNIEAKVVDWITGALLPPGGSGELWIRGPGVMKEYLNGGEATALTIDKDGWLHTG 423
Query: 574 DICYFDNNGYLYLIDRLKEIIKYNGFQIAPAELEAILLSHPEVADVAVTAGRDEAAGEVP 395
D+ Y D++GYLY++DRLKEIIKY GFQIAPA+LEA+L+SH E+ D AV D+ GE+P
Sbjct: 424 DVVYADHDGYLYVVDRLKEIIKYKGFQIAPADLEAVLISHCEILDAAVIPVVDKECGEIP 483
Query: 394 VAFVLRRDGSRISEAALIDYVAKQVSPYKKVRKVVFTSSIPRSAAGKILRRELKSFLPSK 215
VAFV++R GS +++ A+I+YVA+QV+PYKKVRKV+FT SIP+SAAGKILRRELK L SK
Sbjct: 484 VAFVVKRQGSMLTQEAIINYVAEQVAPYKKVRKVIFTQSIPKSAAGKILRRELKCSLTSK 543
Query: 214 I 212
+
Sbjct: 544 L 544
>ref|XP_003612659.1| 4-coumarate-CoA ligase [Medicago truncatula]
gi|355513994|gb|AES95617.1| 4-coumarate-CoA ligase
[Medicago truncatula]
Length = 555
Score = 695 bits (1794), Expect = 0.0
Identities = 349/546 (63%), Positives = 428/546 (78%)
Frame = -3
Query: 1849 QNPHDQPSWFSPQTGVYTSIYTPVNLPSDPFLDVVSFIFSRKHNGVSALIDSSSGFSISY 1670
QN PSW+SP+TG+Y SI++P +LP+DPFLDVV FIFS H+GV ALIDS SG SISY
Sbjct: 11 QNTITHPSWYSPKTGIYNSIHSPRSLPTDPFLDVVCFIFSHPHDGVLALIDSLSGTSISY 70
Query: 1669 QELYSLVRSVASGLQKIGVKQGDVVFILLPNSVYFPVILLGVVYMGAIVSTMNPLSSLSE 1490
L +LV+S+ASGL K+GV QGDVV +LLPNS+Y+P+ LL V+Y+GA+V+ +NPLSS+ E
Sbjct: 71 SNLLTLVKSLASGLHKMGVSQGDVVLLLLPNSIYYPIALLSVMYLGAVVTPLNPLSSVDE 130
Query: 1489 LKKQTLDSHAKFVFTLLKNVDKLSALGVSAIAVPERFDFDSIPSEFSCFYDLISSDSSLM 1310
++K+ D F FT+ +NV KL LG+ I+VPE + D + FSCF++LIS + L
Sbjct: 131 IRKRVNDCGVSFAFTIPENVKKLEPLGIPIISVPEN-EKDLKHACFSCFFNLISGNFVLP 189
Query: 1309 TKPIIKQQDTAAIMHSSGTTGTSKGVVLTHMNFIAMVELFVRFEASQYQLPSWENVYLAV 1130
K +IKQ+DTAAI+ SSGTTG SKGVVLTH N IAMVEL RFEAS+Y+ S +NVYL V
Sbjct: 190 QKSVIKQEDTAAILFSSGTTGVSKGVVLTHRNLIAMVELSARFEASKYEYSSSKNVYLDV 249
Query: 1129 LPMFHIYGLSLFVMGLLSLGSSIVVMSRYNVDEMPTVIDRYGVTHFPVVPPLLAALTKAA 950
LPMFH+YGLSLF GLLSLGS+IVVM ++++DE VID+Y VTHF VVPP+L ALT A
Sbjct: 250 LPMFHLYGLSLFATGLLSLGSTIVVMRKFDIDETIRVIDKYNVTHFHVVPPILTALTAKA 309
Query: 949 HTVARSKLKSLKQVSCGAASLNTKSIQDFVRALPHVDFIQGYGMTESTAVATRGFNTESL 770
V SKL+SL+QV AA L TK+I DFV+A PHVDFIQGYGMTES V GFNTE
Sbjct: 310 KGVNGSKLQSLRQVGSSAAPLTTKAINDFVQAFPHVDFIQGYGMTESGTVGACGFNTEKF 369
Query: 769 HNYSSVGLLSPNMEAKIVDSLTGVFKGPGGMGELWLRGPSIMKGYLNNVEETLSTIDKEG 590
NYSS+GLL+PNMEAK+VD +G F PG GELWLRGPS MKGYLNN E T+ST+DK+G
Sbjct: 370 RNYSSLGLLAPNMEAKVVDWNSGAFLPPGRSGELWLRGPSTMKGYLNNEEATMSTVDKDG 429
Query: 589 WLHTGDICYFDNNGYLYLIDRLKEIIKYNGFQIAPAELEAILLSHPEVADVAVTAGRDEA 410
WLHTGDI YFD +GYLYL DRLKE IKY GFQI PA+LEA+L+ HPE+ADVAVT +DE
Sbjct: 430 WLHTGDIVYFDQDGYLYLSDRLKEFIKYKGFQIPPADLEAVLILHPEIADVAVTGAKDEE 489
Query: 409 AGEVPVAFVLRRDGSRISEAALIDYVAKQVSPYKKVRKVVFTSSIPRSAAGKILRRELKS 230
AGE+PVAFV+++ GS +S +ID+VAKQV+P+KKVRKVVFT IPRS GKILR++L++
Sbjct: 490 AGEIPVAFVVKKVGSVLSPKDVIDFVAKQVAPFKKVRKVVFTDKIPRSVTGKILRKQLRN 549
Query: 229 FLPSKI 212
LPSK+
Sbjct: 550 CLPSKL 555
>ref|XP_003612661.1| 4-coumarate CoA ligase [Medicago truncatula]
gi|355513996|gb|AES95619.1| 4-coumarate CoA ligase
[Medicago truncatula]
Length = 568
Score = 692 bits (1787), Expect = 0.0
Identities = 350/567 (61%), Positives = 437/567 (77%), Gaps = 12/567 (2%)
Frame = -3
Query: 1876 TNVNKISNIQNPHDQPSWFSPQTGVYTSIYTPVNLPSDPFLDVVSFIFSRKHNGVSALID 1697
TN N S + P W+SP+ G+Y+S+ +P NLP+DPFLDVVSFIFS H+GV ALID
Sbjct: 3 TNWNFFSIPNSTITYPHWYSPKPGIYSSLQSPRNLPTDPFLDVVSFIFSHPHDGVLALID 62
Query: 1696 SSSGFSISYQELYSLVRSVASGLQKIGVKQGDVVFILLPNSVYFPVILLGVVYMGAIVST 1517
SSSG SISY L +LV+S+ASGL K+GV QGDVV +LLPNS+Y+P++LL V+Y+GA+V+
Sbjct: 63 SSSGTSISYSNLLTLVKSLASGLHKMGVSQGDVVLLLLPNSIYYPIVLLSVMYLGAVVTP 122
Query: 1516 MNPLSSLSELKKQTLDSHAKFVFTLLKNVDKLSALGVSAIAVPERFDFDSIPSEFSCFYD 1337
+NP SS+ E++KQ + F FT+ +N+ KL +LG+ IAVPE + D FSCFY+
Sbjct: 123 LNPFSSVGEIRKQVKECRVSFSFTIPENIKKLESLGIPVIAVPEN-EMDLKHDCFSCFYN 181
Query: 1336 L------------ISSDSSLMTKPIIKQQDTAAIMHSSGTTGTSKGVVLTHMNFIAMVEL 1193
L I D L +P+IKQ+DTA I++SSGTTG SKGVVLTH N IAMVEL
Sbjct: 182 LTYGKFYSIKKNLIYGDFDLPQRPVIKQEDTAGILYSSGTTGVSKGVVLTHRNLIAMVEL 241
Query: 1192 FVRFEASQYQLPSWENVYLAVLPMFHIYGLSLFVMGLLSLGSSIVVMSRYNVDEMPTVID 1013
FVRFEASQY S + V+LAVLPMFH+YGL+LF GLLSLGS+IVV+ +Y++DE ID
Sbjct: 242 FVRFEASQYDYSSSKTVHLAVLPMFHLYGLALFATGLLSLGSTIVVIRKYDIDEAIRAID 301
Query: 1012 RYGVTHFPVVPPLLAALTKAAHTVARSKLKSLKQVSCGAASLNTKSIQDFVRALPHVDFI 833
+Y VTHF VVP +L+ALT A SKL+SL+ VSCGA L+ +I+DFV+A P+VDFI
Sbjct: 302 KYKVTHFHVVPMMLSALTAKAKDGNGSKLQSLRHVSCGAEPLSKGAIKDFVQAFPNVDFI 361
Query: 832 QGYGMTESTAVATRGFNTESLHNYSSVGLLSPNMEAKIVDSLTGVFKGPGGMGELWLRGP 653
QGYGMTES AV TRGFNT+ HNYSS+GLL+PN+EAK+VD +G F PG GELWLRGP
Sbjct: 362 QGYGMTESGAVGTRGFNTDKFHNYSSLGLLAPNIEAKVVDWKSGTFLPPGLSGELWLRGP 421
Query: 652 SIMKGYLNNVEETLSTIDKEGWLHTGDICYFDNNGYLYLIDRLKEIIKYNGFQIAPAELE 473
SIMKGYLNN E T+STIDK+GW+HTGDI YFD +GYLY+ RLK+IIKY GFQIAPA+LE
Sbjct: 422 SIMKGYLNNEEATMSTIDKDGWIHTGDIVYFDQDGYLYMSGRLKDIIKYKGFQIAPADLE 481
Query: 472 AILLSHPEVADVAVTAGRDEAAGEVPVAFVLRRDGSRISEAALIDYVAKQVSPYKKVRKV 293
A+L+SHPE+ D AVTAG+ + AGE+PVAFV+++ GS +S +IDYVAKQV+PYKKVRKV
Sbjct: 482 ALLISHPEIVDAAVTAGKVDVAGEIPVAFVVKKVGSVLSSQHVIDYVAKQVAPYKKVRKV 541
Query: 292 VFTSSIPRSAAGKILRRELKSFLPSKI 212
VFT IPRSA GKILR+EL++ L SK+
Sbjct: 542 VFTDKIPRSATGKILRKELRNCLTSKL 568
>ref|XP_002304363.1| acyl:coa ligase [Populus trichocarpa] gi|222841795|gb|EEE79342.1|
acyl:coa ligase [Populus trichocarpa]
Length = 557
Score = 691 bits (1782), Expect = 0.0
Identities = 350/554 (63%), Positives = 428/554 (77%), Gaps = 14/554 (2%)
Frame = -3
Query: 1831 PSWFSPQTGVYTSIYTPVNLPSDPFLDVVSFIFSR-KHNGVSALIDSSSGFSISYQELYS 1655
P W+SP+TG+Y+S + P+ LP+DPFLDVVSFIFS HNG++ALIDSSSGFSISY ++
Sbjct: 14 PEWYSPETGIYSSKHPPIPLPADPFLDVVSFIFSHHNHNGLTALIDSSSGFSISYSKILP 73
Query: 1654 LVRSVASGLQKIGVKQGDVVFILLPNSVYFPVILLGVVYMGAIVSTMNPLSSLSELKKQT 1475
LV+SVASGL +G+KQGDVV +LLPNS++FP++ LGV+Y+G IVSTMNPLSS E+K++
Sbjct: 74 LVKSVASGLSNMGIKQGDVVLLLLPNSIHFPIVFLGVLYLGGIVSTMNPLSSELEVKQRI 133
Query: 1474 LDSHAKFVFTLLKNVDKLSALGVSAIAVPERFDFDSIPSEFSCFYDLISSDSSLMTKPII 1295
+D +A F L+ V K LG+ I VPE +FD + I S + KP+I
Sbjct: 134 VDCNACIAFVELEKVCKFQPLGIPVIGVPENVNFD----------EKIYSKGDVGVKPVI 183
Query: 1294 KQQDTAAIMHSSGTTGTSKGVVLTHMNFIAMVELFVRFEASQYQLPSWENVYLAVLPMFH 1115
+QQDTAAIM+SSGTT SKGVVLTH NFI+MVELFV+FEASQY+ S +NVYLAVLPMFH
Sbjct: 184 RQQDTAAIMYSSGTTAASKGVVLTHRNFISMVELFVKFEASQYEYSSTDNVYLAVLPMFH 243
Query: 1114 IYGLSLFVMGLLSLGSSIVVMSRYNVDEMPTVIDRYGVTHFPVVPPLLAALTKAAHTVAR 935
IYGLSLFV+GLLSLGSSIVVM +++V EM VIDRYGVTHFPVVPP+L ALT+ A V
Sbjct: 244 IYGLSLFVVGLLSLGSSIVVMRKFDVSEMVKVIDRYGVTHFPVVPPILTALTRTAKGVCG 303
Query: 934 SKLKSLKQVSCGAASLNTKSIQDFVRALPHVDFIQGYGMTESTAVATRGFNTESLHNYSS 755
+ LK LK VSCGAA L K+IQDFV LPHVDFIQGYG+TESTAV TRG NTE YSS
Sbjct: 304 NSLKCLKLVSCGAAPLFGKTIQDFVEVLPHVDFIQGYGLTESTAVGTRGLNTEKFQKYSS 363
Query: 754 VGLLSPNMEAKIVDSLTGVFKGPGGMGELWLRGPSIMKGYLNNVEETLSTIDKEGWLHTG 575
+GLL+PN+EAK+VD +TG PGG GELW+RGP +MK YLN E T TIDK+GWLHTG
Sbjct: 364 IGLLAPNIEAKVVDWITGALLPPGGSGELWIRGPGVMKEYLNGGEATALTIDKDGWLHTG 423
Query: 574 DICYFDNNGYLYLIDRLKEIIKYNGF-------------QIAPAELEAILLSHPEVADVA 434
D+ Y D++GYLY++DRLKEIIKY GF QIAPA+LEA+L+SH E+ D A
Sbjct: 424 DVVYADHDGYLYVVDRLKEIIKYKGFQFVDLYVTILSLLQIAPADLEAVLISHCEILDAA 483
Query: 433 VTAGRDEAAGEVPVAFVLRRDGSRISEAALIDYVAKQVSPYKKVRKVVFTSSIPRSAAGK 254
V D+ GE+PVAFV++R GS +++ A+I+YVA+QV+PYKKVRKV+FT SIP+SAAGK
Sbjct: 484 VIPVVDKECGEIPVAFVVKRQGSMLTQEAIINYVAEQVAPYKKVRKVIFTQSIPKSAAGK 543
Query: 253 ILRRELKSFLPSKI 212
ILRRELK L SK+
Sbjct: 544 ILRRELKCSLTSKL 557